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The map label for this gene is trmE

Identifier: 146300457

GI number: 146300457

Start: 3220036

End: 3221436

Strand: Reverse

Name: trmE

Synonym: Fjoh_2707

Alternate gene names: 146300457

Gene position: 3221436-3220036 (Counterclockwise)

Preceding gene: 146300468

Following gene: 146300456

Centisome position: 52.84

GC content: 33.55

Gene sequence:

>1401_bases
ATGATAAATCAAGATTCTATAGTTGCATTAGCTACTCCATCAGGAGCCGGAGCTATTGCTGTTATTCGTATTTCGGGCGC
TGAAGCTGTTTCAATAGGAAATTCTGTTTTTAAATCTATCAAAAACAAAGACTTAACGAAACAAAAAACGCATACTTTGC
ATTTGGGCCATATTGTTGACAACGGAAAAACACTTGATGAAGTTTTGGTTTCTGTTTTTAAAGGCCCTAATTCGTATACT
GGAGAAGATACAATAGAAATTTCATGCCACGGATCTACATATATTCAGCAGCAGATTATTCAATTATTATTGAGAAAAGG
CTGCAGAATGGCCGATGCCGGTGAATTTACTTTAAGAGCTTTCTTAAATGGGAAGTTAGATTTATCTCAGGCAGAAGCTG
TTGCAGATTTAATTTCATCTGATAATGAAGCTTCACACCACATTGCCATGCAGCAAATGCGAGGTGGTTTCAGCAATGAA
ATTGCCAAATTGCGAGAAGAGCTTTTAAATTTTGCTTCTTTGATTGAACTTGAATTGGACTTTGCAGAGGAAGATGTGGA
ATTTGCCGACAGAACTCAGTTTCATGAATTATTAAACAGAATTGAATTTGTTTTAAAACGTCTAATAGATTCATTTGCAG
TTGGAAATGTTATTAAAAACGGAATCCCGGTTGCCATTGTTGGTGAGCCGAATGTTGGTAAATCGACTTTATTAAATGCT
TTATTAAATGAAGAGCGAGCGATTGTTTCTGACATTGCCGGAACAACGCGCGATACAATTGAAGATGAGCTGGTTATTGG
AGGTATTGGTTTTAGATTTATTGATACAGCCGGAATACGCGAAACGAAAGATGTTGTAGAAAGTATTGGAATCAAGAAAA
CTTTCGAAAAAATAGATCAGGCACAAGTTGTTATTTATTTGTTTGATGGTTTAAAATTTAAAGCATCGAGTTCTGAATTT
GTTTCGGAAATTGAACAAATTAAAAACAAATATCCTTTAAAACCGCTTCTTATAGTGGTCAATAAAAAGGATATTTTATC
TGAAGATGAAGTTTTAAATATTACACAAAAGCTCGAAAACTTAAACGCGAAACTTCTATTAATTTCGGCAAAACAAAAAA
TTGGAGTTGAAGAATTAAAGAACGAATTATTATCATTTGTTAATACTGGAGCATTACGAAATAATGAAACAATTGTTACA
AATACAAGACATTATGACTCTTTACTGAAAGCTTTAGATGAAATACAAAAAGTAAAATTTGGTCTTGAAACCAATCTTTC
AAGTGACTTAATTGCACTTGATATTAAAGAAGCCTTATATCAATTCGGACTTATCACAGGTCAGGTTTCCAACGACGAAT
TACTAGGGAATATCTTTGCAAATTTCTGTATTGGCAAGTAA

Upstream 100 bases:

>100_bases
TAATTTTTTCTTAATAAAACACTATTTTGTGTAGAGAATCTGATCAAACTGCCGGTTATTAAGACTTTACAATTTTCTGC
TTTTTTCATACCTTTGCAGC

Downstream 100 bases:

>100_bases
AGGGACAAAATATCACAAAATAAAAACAAATAATCGAGTTAAGGTATTGATAAACAATGTTTTAACTCGATTTTTATATT
CGTTTTCTTTTGGATATTAG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MINQDSIVALATPSGAGAIAVIRISGAEAVSIGNSVFKSIKNKDLTKQKTHTLHLGHIVDNGKTLDEVLVSVFKGPNSYT
GEDTIEISCHGSTYIQQQIIQLLLRKGCRMADAGEFTLRAFLNGKLDLSQAEAVADLISSDNEASHHIAMQQMRGGFSNE
IAKLREELLNFASLIELELDFAEEDVEFADRTQFHELLNRIEFVLKRLIDSFAVGNVIKNGIPVAIVGEPNVGKSTLLNA
LLNEERAIVSDIAGTTRDTIEDELVIGGIGFRFIDTAGIRETKDVVESIGIKKTFEKIDQAQVVIYLFDGLKFKASSSEF
VSEIEQIKNKYPLKPLLIVVNKKDILSEDEVLNITQKLENLNAKLLLISAKQKIGVEELKNELLSFVNTGALRNNETIVT
NTRHYDSLLKALDEIQKVKFGLETNLSSDLIALDIKEALYQFGLITGQVSNDELLGNIFANFCIGK

Sequences:

>Translated_466_residues
MINQDSIVALATPSGAGAIAVIRISGAEAVSIGNSVFKSIKNKDLTKQKTHTLHLGHIVDNGKTLDEVLVSVFKGPNSYT
GEDTIEISCHGSTYIQQQIIQLLLRKGCRMADAGEFTLRAFLNGKLDLSQAEAVADLISSDNEASHHIAMQQMRGGFSNE
IAKLREELLNFASLIELELDFAEEDVEFADRTQFHELLNRIEFVLKRLIDSFAVGNVIKNGIPVAIVGEPNVGKSTLLNA
LLNEERAIVSDIAGTTRDTIEDELVIGGIGFRFIDTAGIRETKDVVESIGIKKTFEKIDQAQVVIYLFDGLKFKASSSEF
VSEIEQIKNKYPLKPLLIVVNKKDILSEDEVLNITQKLENLNAKLLLISAKQKIGVEELKNELLSFVNTGALRNNETIVT
NTRHYDSLLKALDEIQKVKFGLETNLSSDLIALDIKEALYQFGLITGQVSNDELLGNIFANFCIGK
>Mature_466_residues
MINQDSIVALATPSGAGAIAVIRISGAEAVSIGNSVFKSIKNKDLTKQKTHTLHLGHIVDNGKTLDEVLVSVFKGPNSYT
GEDTIEISCHGSTYIQQQIIQLLLRKGCRMADAGEFTLRAFLNGKLDLSQAEAVADLISSDNEASHHIAMQQMRGGFSNE
IAKLREELLNFASLIELELDFAEEDVEFADRTQFHELLNRIEFVLKRLIDSFAVGNVIKNGIPVAIVGEPNVGKSTLLNA
LLNEERAIVSDIAGTTRDTIEDELVIGGIGFRFIDTAGIRETKDVVESIGIKKTFEKIDQAQVVIYLFDGLKFKASSSEF
VSEIEQIKNKYPLKPLLIVVNKKDILSEDEVLNITQKLENLNAKLLLISAKQKIGVEELKNELLSFVNTGALRNNETIVT
NTRHYDSLLKALDEIQKVKFGLETNLSSDLIALDIKEALYQFGLITGQVSNDELLGNIFANFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=30.3418803418803, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=28.6, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=30.2375809935205, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI2367268, Length=471, Percent_Identity=36.0934182590234, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI87082120, Length=131, Percent_Identity=42.7480916030534, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17507259, Length=473, Percent_Identity=29.5983086680761, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=507, Percent_Identity=31.7554240631164, Blast_Score=190, Evalue=4e-49,
Organism=Drosophila melanogaster, GI45550813, Length=476, Percent_Identity=29.8319327731092, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_FLAJ1 (A5FGE0)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195048.1
- ProteinModelPortal:   A5FGE0
- SMR:   A5FGE0
- STRING:   A5FGE0
- GeneID:   5090503
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2707
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   FJOH376686:FJOH_2707-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51305; Mature: 51305

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 86-86 BINDING 125-125 BINDING 466-466

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINQDSIVALATPSGAGAIAVIRISGAEAVSIGNSVFKSIKNKDLTKQKTHTLHLGHIVD
CCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHCEEEEEEEEC
NGKTLDEVLVSVFKGPNSYTGEDTIEISCHGSTYIQQQIIQLLLRKGCRMADAGEFTLRA
CCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHH
FLNGKLDLSQAEAVADLISSDNEASHHIAMQQMRGGFSNEIAKLREELLNFASLIELELD
HHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
FAEEDVEFADRTQFHELLNRIEFVLKRLIDSFAVGNVIKNGIPVAIVGEPNVGKSTLLNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
LLNEERAIVSDIAGTTRDTIEDELVIGGIGFRFIDTAGIRETKDVVESIGIKKTFEKIDQ
HHHHHHHHHHHHCCCCHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHCCHHHHHHHHCC
AQVVIYLFDGLKFKASSSEFVSEIEQIKNKYPLKPLLIVVNKKDILSEDEVLNITQKLEN
CEEEEEEECCCEEECCCHHHHHHHHHHHHCCCCCEEEEEECCHHCCCCHHHHHHHHHHHC
LNAKLLLISAKQKIGVEELKNELLSFVNTGALRNNETIVTNTRHYDSLLKALDEIQKVKF
CCCEEEEEECHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHH
GLETNLSSDLIALDIKEALYQFGLITGQVSNDELLGNIFANFCIGK
CCCCCCCCCCEEHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINQDSIVALATPSGAGAIAVIRISGAEAVSIGNSVFKSIKNKDLTKQKTHTLHLGHIVD
CCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHCEEEEEEEEC
NGKTLDEVLVSVFKGPNSYTGEDTIEISCHGSTYIQQQIIQLLLRKGCRMADAGEFTLRA
CCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHH
FLNGKLDLSQAEAVADLISSDNEASHHIAMQQMRGGFSNEIAKLREELLNFASLIELELD
HHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
FAEEDVEFADRTQFHELLNRIEFVLKRLIDSFAVGNVIKNGIPVAIVGEPNVGKSTLLNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
LLNEERAIVSDIAGTTRDTIEDELVIGGIGFRFIDTAGIRETKDVVESIGIKKTFEKIDQ
HHHHHHHHHHHHCCCCHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHCCHHHHHHHHCC
AQVVIYLFDGLKFKASSSEFVSEIEQIKNKYPLKPLLIVVNKKDILSEDEVLNITQKLEN
CEEEEEEECCCEEECCCHHHHHHHHHHHHCCCCCEEEEEECCHHCCCCHHHHHHHHHHHC
LNAKLLLISAKQKIGVEELKNELLSFVNTGALRNNETIVTNTRHYDSLLKALDEIQKVKF
CCCEEEEEECHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHH
GLETNLSSDLIALDIKEALYQFGLITGQVSNDELLGNIFANFCIGK
CCCCCCCCCCEEHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA