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The map label for this gene is ddl

Identifier: 146300373

GI number: 146300373

Start: 3113809

End: 3114780

Strand: Reverse

Name: ddl

Synonym: Fjoh_2618

Alternate gene names: 146300373

Gene position: 3114780-3113809 (Counterclockwise)

Preceding gene: 146300376

Following gene: 146300372

Centisome position: 51.09

GC content: 37.04

Gene sequence:

>972_bases
ATGAAAAACATTGCCATCATCATGGGCGGATATTCAAGCGAATACAAAATTTCGCTTATCAGCGGAAACGTTGTGTACCA
ATATCTTGACAAAACAAAATACAACGGATTCCGAATTCATATCTTTAAAGAGAAATGGGTTTATGTAGACGCAAATGACG
CCGAATTTCCAATTGACAGAAACGATTTTTCTGTAACGGTAAACGGCGAAAAAATTACTTTCGACTGCGTTTTCAATGCC
ATTCACGGAACTCCGGGCGAAGATGGATTAATGCAGGCTTATTTTGAATTAATTGGATTGCCGCAGTCTTCATGCGATTA
TTACCAATCGGCATTAACATTTAATAAAAGAGATTTATTATCGGTTTTAAAACCATACGGAATCAAAACGGCAATTTCTT
ATTACTTAAACAAAGGCGACGAAATCAATACTGCCGAAATCGTTAAAAAAGTTGGGCTTCCTTGTTTCGTAAAACCAAAC
AAAGCGGGTTCTAGTTTCGGAATTTCTAAAGTAAAATCTGAGGCCGAACTTCCAATTGCAATTGAAGTTGCGTATAAAGA
AGATAACGAAATCATCATCGAAAGTTTCCTTGACGGAACTGAAGTTTCTGTCGGCGTAATCAATTACAAAGGCGAAATCA
AAGTATTGCCAATTACAGAAATTGTTTCAGACAATGATTTCTTCGATTACGAGGCAAAATACGAAGGAAAATCACAAGAA
ATAACACCAGCGAGAATCTCAGACGAGCTGACTCAAAAAGTGGGAGAAACTGCAAAACGTGCTTACGAAGTTTTAAAAAT
GAAAGGATTCTCACGAAGCGAATTCATCATTGTAGACAACGAACCGTATATGCTTGAAATGAACACTATTCCGGGTTTAA
CAACCGAAAGTTTGATTCCGCAGCAAGCAAAAGCAGCCGGAATTTCTCTGGAAGATTTATTTACAAATGCTATTGAATTA
TCGCTTGCATAA

Upstream 100 bases:

>100_bases
ACACAGATATAAACCGATTGAAAAAAAACAATTTGCGGCGATTTGTGTAATGCGCAGAAAAAATAAACAAATTAACGATT
AAACAAATAAATAAAAGTCA

Downstream 100 bases:

>100_bases
AGGTTCTAAGATACTAAGGTGCTAAGGTTATAAGATCTTAGCACCTTATTTTTTAAACCTATAAATATCAGACTAAACAA
AGTAAAGTCAAAACCTTAGA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKNIAIIMGGYSSEYKISLISGNVVYQYLDKTKYNGFRIHIFKEKWVYVDANDAEFPIDRNDFSVTVNGEKITFDCVFNA
IHGTPGEDGLMQAYFELIGLPQSSCDYYQSALTFNKRDLLSVLKPYGIKTAISYYLNKGDEINTAEIVKKVGLPCFVKPN
KAGSSFGISKVKSEAELPIAIEVAYKEDNEIIIESFLDGTEVSVGVINYKGEIKVLPITEIVSDNDFFDYEAKYEGKSQE
ITPARISDELTQKVGETAKRAYEVLKMKGFSRSEFIIVDNEPYMLEMNTIPGLTTESLIPQQAKAAGISLEDLFTNAIEL
SLA

Sequences:

>Translated_323_residues
MKNIAIIMGGYSSEYKISLISGNVVYQYLDKTKYNGFRIHIFKEKWVYVDANDAEFPIDRNDFSVTVNGEKITFDCVFNA
IHGTPGEDGLMQAYFELIGLPQSSCDYYQSALTFNKRDLLSVLKPYGIKTAISYYLNKGDEINTAEIVKKVGLPCFVKPN
KAGSSFGISKVKSEAELPIAIEVAYKEDNEIIIESFLDGTEVSVGVINYKGEIKVLPITEIVSDNDFFDYEAKYEGKSQE
ITPARISDELTQKVGETAKRAYEVLKMKGFSRSEFIIVDNEPYMLEMNTIPGLTTESLIPQQAKAAGISLEDLFTNAIEL
SLA
>Mature_323_residues
MKNIAIIMGGYSSEYKISLISGNVVYQYLDKTKYNGFRIHIFKEKWVYVDANDAEFPIDRNDFSVTVNGEKITFDCVFNA
IHGTPGEDGLMQAYFELIGLPQSSCDYYQSALTFNKRDLLSVLKPYGIKTAISYYLNKGDEINTAEIVKKVGLPCFVKPN
KAGSSFGISKVKSEAELPIAIEVAYKEDNEIIIESFLDGTEVSVGVINYKGEIKVLPITEIVSDNDFFDYEAKYEGKSQE
ITPARISDELTQKVGETAKRAYEVLKMKGFSRSEFIIVDNEPYMLEMNTIPGLTTESLIPQQAKAAGISLEDLFTNAIEL
SLA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=347, Percent_Identity=31.4121037463977, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1786280, Length=327, Percent_Identity=33.0275229357798, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_FLAJ1 (A5FGN3)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194964.1
- ProteinModelPortal:   A5FGN3
- SMR:   A5FGN3
- STRING:   A5FGN3
- GeneID:   5093513
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2618
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   VPYTGSD
- ProtClustDB:   PRK01966
- BioCyc:   FJOH376686:FJOH_2618-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 36085; Mature: 36085

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIAIIMGGYSSEYKISLISGNVVYQYLDKTKYNGFRIHIFKEKWVYVDANDAEFPIDR
CCCEEEEECCCCCCEEEEEEECCEEEEEHHCCCCCCEEEEEEEEEEEEEECCCCCCCCCC
NDFSVTVNGEKITFDCVFNAIHGTPGEDGLMQAYFELIGLPQSSCDYYQSALTFNKRDLL
CCEEEEECCCEEEEEEEEEHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHH
SVLKPYGIKTAISYYLNKGDEINTAEIVKKVGLPCFVKPNKAGSSFGISKVKSEAELPIA
HHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCEEECCCCCCCCCCHHHHCCCCCCCEE
IEVAYKEDNEIIIESFLDGTEVSVGVINYKGEIKVLPITEIVSDNDFFDYEAKYEGKSQE
EEEEEECCCEEEEEECCCCCEEEEEEEEECCCEEEEEEHHHHCCCCCEEEEEECCCCCCC
ITPARISDELTQKVGETAKRAYEVLKMKGFSRSEFIIVDNEPYMLEMNTIPGLTTESLIP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCCCCHHHCCC
QQAKAAGISLEDLFTNAIELSLA
CHHHHCCCCHHHHHCCCEEEEEC
>Mature Secondary Structure
MKNIAIIMGGYSSEYKISLISGNVVYQYLDKTKYNGFRIHIFKEKWVYVDANDAEFPIDR
CCCEEEEECCCCCCEEEEEEECCEEEEEHHCCCCCCEEEEEEEEEEEEEECCCCCCCCCC
NDFSVTVNGEKITFDCVFNAIHGTPGEDGLMQAYFELIGLPQSSCDYYQSALTFNKRDLL
CCEEEEECCCEEEEEEEEEHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHH
SVLKPYGIKTAISYYLNKGDEINTAEIVKKVGLPCFVKPNKAGSSFGISKVKSEAELPIA
HHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCEEECCCCCCCCCCHHHHCCCCCCCEE
IEVAYKEDNEIIIESFLDGTEVSVGVINYKGEIKVLPITEIVSDNDFFDYEAKYEGKSQE
EEEEEECCCEEEEEECCCCCEEEEEEEEECCCEEEEEEHHHHCCCCCEEEEEECCCCCCC
ITPARISDELTQKVGETAKRAYEVLKMKGFSRSEFIIVDNEPYMLEMNTIPGLTTESLIP
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCCCCHHHCCC
QQAKAAGISLEDLFTNAIELSLA
CHHHHCCCCHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA