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The map label for this gene is prfB [H]

Identifier: 146300308

GI number: 146300308

Start: 3036191

End: 3036955

Strand: Reverse

Name: prfB [H]

Synonym: Fjoh_2553

Alternate gene names: 146300308

Gene position: 3036955-3036191 (Counterclockwise)

Preceding gene: 146300310

Following gene: 146300307

Centisome position: 49.81

GC content: 39.87

Gene sequence:

>765_bases
ATGCTTTCTGACGAAGGTGACAGTTTAAGTGCAGTAGTTCAAATTACGGCCGGAGCCGGAGGAACAGAAAGTTGTGACTG
GGCCGGAATGCTGATGCGTATGTACATGATGTGGGGAGAAAGTTATGGTTACAAAATAAAAGAACTTAACTTTCAGGAAG
GTGATGTAGCAGGAATTAAAACTGTAACTTTAGAGTTCGAAGGCGATTATTCTTTTGGGTATTTAAAAGGCGAAAACGGA
GTTCACCGTCTGGTTAGAATTTCTCCTTTTGATAGTAATGCTAAACGTCATACTTCATTCGTGTCTGTATATGTTTATCC
GCTTGTTGATGACAGTATCGAAATTGACATTAATCCTGCCGATATTGAAATTACAACGTCACGATCAAGTGGTGCCGGAG
GACAGAACGTAAATAAAGTTGAAACAAAAGTTCAATTAGTACATAAACCAACCGGAATCCAGATTCAGTGTTCTGAGACA
AGATCACAACAGGACAACAGACAGCGTGCTATGCAGATGCTTCGTTCTCAATTGTATGAAATTGAGTTAAAGAAACAACA
AGCTCAACGTGCCGATATTGAAGCCGGAAAAATGAAAATCGAATGGGGTTCGCAGATTCGTAACTACGTTATGCAGCCTT
ATAAATTAGTAAAAGACGTTCGTACAGGTTATGAAACCAGCGATGTCGACGGCGTTATGAATGGAAATATAGATGCATTC
CTGAAAGCCTATTTAATGATGATGGGACAGAAGGAAGAGGAATAG

Upstream 100 bases:

>100_bases
TGCTTATGATTTTTATAAAGAAGGTGAGCTTTCTACAGAAGAATTAGACGAACAATACAATGTAACTCAGTCTCATATAG
AAAACATCGAATTCAAAAAC

Downstream 100 bases:

>100_bases
TATTCAGTCGCAGTCTCAGTTTTCAATAAACTGAGACTGTACTGAGACTGAAAACTGAGACTGAAAACTAAAAAAAATAG
TTATGATAAAATGGGCAGAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLSDEGDSLSAVVQITAGAGGTESCDWAGMLMRMYMMWGESYGYKIKELNFQEGDVAGIKTVTLEFEGDYSFGYLKGENG
VHRLVRISPFDSNAKRHTSFVSVYVYPLVDDSIEIDINPADIEITTSRSSGAGGQNVNKVETKVQLVHKPTGIQIQCSET
RSQQDNRQRAMQMLRSQLYEIELKKQQAQRADIEAGKMKIEWGSQIRNYVMQPYKLVKDVRTGYETSDVDGVMNGNIDAF
LKAYLMMMGQKEEE

Sequences:

>Translated_254_residues
MLSDEGDSLSAVVQITAGAGGTESCDWAGMLMRMYMMWGESYGYKIKELNFQEGDVAGIKTVTLEFEGDYSFGYLKGENG
VHRLVRISPFDSNAKRHTSFVSVYVYPLVDDSIEIDINPADIEITTSRSSGAGGQNVNKVETKVQLVHKPTGIQIQCSET
RSQQDNRQRAMQMLRSQLYEIELKKQQAQRADIEAGKMKIEWGSQIRNYVMQPYKLVKDVRTGYETSDVDGVMNGNIDAF
LKAYLMMMGQKEEE
>Mature_254_residues
MLSDEGDSLSAVVQITAGAGGTESCDWAGMLMRMYMMWGESYGYKIKELNFQEGDVAGIKTVTLEFEGDYSFGYLKGENG
VHRLVRISPFDSNAKRHTSFVSVYVYPLVDDSIEIDINPADIEITTSRSSGAGGQNVNKVETKVQLVHKPTGIQIQCSET
RSQQDNRQRAMQMLRSQLYEIELKKQQAQRADIEAGKMKIEWGSQIRNYVMQPYKLVKDVRTGYETSDVDGVMNGNIDAF
LKAYLMMMGQKEEE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=239, Percent_Identity=31.3807531380753, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI34577120, Length=240, Percent_Identity=32.0833333333333, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=31.5436241610738, Blast_Score=95, Evalue=5e-20,
Organism=Escherichia coli, GI2367172, Length=245, Percent_Identity=51.4285714285714, Blast_Score=274, Evalue=5e-75,
Organism=Escherichia coli, GI1787462, Length=247, Percent_Identity=34.8178137651822, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17542784, Length=219, Percent_Identity=29.6803652968037, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321295, Length=243, Percent_Identity=28.3950617283951, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19921226, Length=244, Percent_Identity=35.2459016393443, Blast_Score=141, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 28593; Mature: 28593

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDEGDSLSAVVQITAGAGGTESCDWAGMLMRMYMMWGESYGYKIKELNFQEGDVAGIK
CCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEE
TVTLEFEGDYSFGYLKGENGVHRLVRISPFDSNAKRHTSFVSVYVYPLVDDSIEIDINPA
EEEEEEECCCEEEEEECCCCCEEEEEECCCCCCCHHHEEEEEEEEEEEECCCEEEEECCC
DIEITTSRSSGAGGQNVNKVETKVQLVHKPTGIQIQCSETRSQQDNRQRAMQMLRSQLYE
CEEEEECCCCCCCCCCCHHHHHEEEEEECCCCEEEEECCHHCCHHHHHHHHHHHHHHHHH
IELKKQQAQRADIEAGKMKIEWGSQIRNYVMQPYKLVKDVRTGYETSDVDGVMNGNIDAF
HHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LKAYLMMMGQKEEE
HHHHHHHHCCCCCC
>Mature Secondary Structure
MLSDEGDSLSAVVQITAGAGGTESCDWAGMLMRMYMMWGESYGYKIKELNFQEGDVAGIK
CCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEE
TVTLEFEGDYSFGYLKGENGVHRLVRISPFDSNAKRHTSFVSVYVYPLVDDSIEIDINPA
EEEEEEECCCEEEEEECCCCCEEEEEECCCCCCCHHHEEEEEEEEEEEECCCEEEEECCC
DIEITTSRSSGAGGQNVNKVETKVQLVHKPTGIQIQCSETRSQQDNRQRAMQMLRSQLYE
CEEEEECCCCCCCCCCCHHHHHEEEEEECCCCEEEEECCHHCCHHHHHHHHHHHHHHHHH
IELKKQQAQRADIEAGKMKIEWGSQIRNYVMQPYKLVKDVRTGYETSDVDGVMNGNIDAF
HHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LKAYLMMMGQKEEE
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]