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The map label for this gene is lpxD1 [H]

Identifier: 146300306

GI number: 146300306

Start: 3034329

End: 3035327

Strand: Reverse

Name: lpxD1 [H]

Synonym: Fjoh_2551

Alternate gene names: 146300306

Gene position: 3035327-3034329 (Counterclockwise)

Preceding gene: 146300307

Following gene: 146300301

Centisome position: 49.78

GC content: 38.74

Gene sequence:

>999_bases
ATGAGATCCTATTCAATTCAAGAAATTAATGAAGTAATTAATGGCGTAATTTACGGCACTACTTCGCAGAACATTACCGC
AACTGAGCAATTAGAAAAAGCTACAACTTCAGAGATTTCTTTTATAGGAAATAAAAAATACGAAAAATACTGGTCGACAT
CAAATGCTTCAATTGCAGTTGTAAACGAGGATATTTCGATTGAACCTGGTGAAAATCGCGCTTTTATTAAGGTTAAAAAT
GCAGATTTGGCGATGTCACAAATCCTTGCTCTTTTTGCTCCTCCTACACCAATATTCCATAACAATATACACAAAACCGC
CACTATAGACGAAACAGCTGTAATTGGTGAAGGTGCTAAAATTGGTGCGGGATGTTATATTGGACCTAAAGTTGAAATTG
GTGCTAATGCAACGATTTACCCAAACGTTACCATTTTGGATGAATGTACTATTGGAAAAAACACCATTATCTGGTCAGGA
AGCGTTGTACGTGAGCGTTGTCATATTGGAAGTGATTGTATTATTCACCCAAATGCTACAATTGGAGCCGATGGTTTTGG
ATTTCGTCCTTGTACTGAAAAAGGTTTAGTAAAAATTCCTCAAATTGGAAACGTAATCATTGGAAACGGAGTTGAAATCG
GAGCCAATTCCTGCGTTGACCGTGGAAAATTCAGTTCGACCGTTTTAGGCGACGGCTGTAAAATTGACAATTTAGTACAA
ATTGGACATAACAGTAAATTAGGCCGATTCTGTATTATGGCTGGAAACAGTGGTTTAGCAGGTTCTGTAACTTTAGGAAA
CGGAGTTATTATTGGCGGAAGCGCTTCAATAAAAGACCATACCACAATTGGTGACGGAGCTGTAATTGGCGCCGGATCTG
GTGTTACTGGAGATGTTCCTGCCGGAAAAACAATGCTGGGTTATCCTGCTGTTGAAGCTCGTGATGCATTAAAACAATGG
GCAATGCTAAAACGAATGGTTTGCGCTCCTAAAAAATAA

Upstream 100 bases:

>100_bases
TTTTAAATATTTATTAAGAAACATAAAAAATTTATTAGGTAATTAAAATCTAATTTGTTTTATTTGGCAAAAATTAACCC
TAAGTCGATTAAAATTGTCA

Downstream 100 bases:

>100_bases
TCTGCAATTCTAAATATTTTTAAACACATAGAAACATAGATTTTGTTTTCGCAAAAGGCAATTTCATTTGCATTAACAAA
TATAGCCTATGTGTAAATGA

Product: UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MRSYSIQEINEVINGVIYGTTSQNITATEQLEKATTSEISFIGNKKYEKYWSTSNASIAVVNEDISIEPGENRAFIKVKN
ADLAMSQILALFAPPTPIFHNNIHKTATIDETAVIGEGAKIGAGCYIGPKVEIGANATIYPNVTILDECTIGKNTIIWSG
SVVRERCHIGSDCIIHPNATIGADGFGFRPCTEKGLVKIPQIGNVIIGNGVEIGANSCVDRGKFSSTVLGDGCKIDNLVQ
IGHNSKLGRFCIMAGNSGLAGSVTLGNGVIIGGSASIKDHTTIGDGAVIGAGSGVTGDVPAGKTMLGYPAVEARDALKQW
AMLKRMVCAPKK

Sequences:

>Translated_332_residues
MRSYSIQEINEVINGVIYGTTSQNITATEQLEKATTSEISFIGNKKYEKYWSTSNASIAVVNEDISIEPGENRAFIKVKN
ADLAMSQILALFAPPTPIFHNNIHKTATIDETAVIGEGAKIGAGCYIGPKVEIGANATIYPNVTILDECTIGKNTIIWSG
SVVRERCHIGSDCIIHPNATIGADGFGFRPCTEKGLVKIPQIGNVIIGNGVEIGANSCVDRGKFSSTVLGDGCKIDNLVQ
IGHNSKLGRFCIMAGNSGLAGSVTLGNGVIIGGSASIKDHTTIGDGAVIGAGSGVTGDVPAGKTMLGYPAVEARDALKQW
AMLKRMVCAPKK
>Mature_332_residues
MRSYSIQEINEVINGVIYGTTSQNITATEQLEKATTSEISFIGNKKYEKYWSTSNASIAVVNEDISIEPGENRAFIKVKN
ADLAMSQILALFAPPTPIFHNNIHKTATIDETAVIGEGAKIGAGCYIGPKVEIGANATIYPNVTILDECTIGKNTIIWSG
SVVRERCHIGSDCIIHPNATIGADGFGFRPCTEKGLVKIPQIGNVIIGNGVEIGANSCVDRGKFSSTVLGDGCKIDNLVQ
IGHNSKLGRFCIMAGNSGLAGSVTLGNGVIIGGSASIKDHTTIGDGAVIGAGSGVTGDVPAGKTMLGYPAVEARDALKQW
AMLKRMVCAPKK

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786376, Length=286, Percent_Identity=34.6153846153846, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1786378, Length=205, Percent_Identity=33.6585365853659, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573 [H]

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 34827; Mature: 34827

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSYSIQEINEVINGVIYGTTSQNITATEQLEKATTSEISFIGNKKYEKYWSTSNASIAV
CCCCCHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHEEEECCCCHHHHCCCCCCEEEE
VNEDISIEPGENRAFIKVKNADLAMSQILALFAPPTPIFHNNIHKTATIDETAVIGEGAK
EECCEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHCCCCCCEEECCCEEEECCCCE
IGAGCYIGPKVEIGANATIYPNVTILDECTIGKNTIIWSGSVVRERCHIGSDCIIHPNAT
ECCCEEECCEEEECCCCEECCCEEEEEECCCCCCEEEECCHHHHHHHCCCCCEEECCCCE
IGADGFGFRPCTEKGLVKIPQIGNVIIGNGVEIGANSCVDRGKFSSTVLGDGCKIDNLVQ
ECCCCCCCCCCCCCCCEECCCCCCEEEECCEEECCCCCCCCCCCCCEEECCCCCCCCEEE
IGHNSKLGRFCIMAGNSGLAGSVTLGNGVIIGGSASIKDHTTIGDGAVIGAGSGVTGDVP
ECCCCCCCEEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCC
AGKTMLGYPAVEARDALKQWAMLKRMVCAPKK
CCCEEECCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRSYSIQEINEVINGVIYGTTSQNITATEQLEKATTSEISFIGNKKYEKYWSTSNASIAV
CCCCCHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHEEEECCCCHHHHCCCCCCEEEE
VNEDISIEPGENRAFIKVKNADLAMSQILALFAPPTPIFHNNIHKTATIDETAVIGEGAK
EECCEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHCCCCCCEEECCCEEEECCCCE
IGAGCYIGPKVEIGANATIYPNVTILDECTIGKNTIIWSGSVVRERCHIGSDCIIHPNAT
ECCCEEECCEEEECCCCEECCCEEEEEECCCCCCEEEECCHHHHHHHCCCCCEEECCCCE
IGADGFGFRPCTEKGLVKIPQIGNVIIGNGVEIGANSCVDRGKFSSTVLGDGCKIDNLVQ
ECCCCCCCCCCCCCCCEECCCCCCEEEECCEEECCCCCCCCCCCCCEEECCCCCCCCEEE
IGHNSKLGRFCIMAGNSGLAGSVTLGNGVIIGGSASIKDHTTIGDGAVIGAGSGVTGDVP
ECCCCCCCEEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCC
AGKTMLGYPAVEARDALKQWAMLKRMVCAPKK
CCCEEECCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA