Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 146300193

GI number: 146300193

Start: 2923793

End: 2924449

Strand: Reverse

Name: nth [H]

Synonym: Fjoh_2438

Alternate gene names: 146300193

Gene position: 2924449-2923793 (Counterclockwise)

Preceding gene: 146300196

Following gene: 146300184

Centisome position: 47.97

GC content: 37.29

Gene sequence:

>657_bases
ATGAATAAAGAAGCCCGCGTACAATTTGTTATAAATACCTTAAAAGAACTCTACCCTACTATACCTGTTCCGCTTGACCA
TAAAGATCCATATACTTTATTAATTGCCGTATTGCTTTCTGCTCAATGCACAGATGTACGTGTGAACCAGATTACACCTC
TTTTGTTTGCAAAAGCAGATAACCCGTATGACATGGTGAAGATGTCGATTGAAGAAATTAAGGAAATTATTCGTCCGTGT
GGTTTATCGCCAATGAAATCAAAAGGAATTCATGGTTTATCTGAAATATTAATTGAAAAACATAATGGAGAAGTACCGCA
GAGTTTTGAAGCGCTTGAAGAATTACCAGCTGTAGGACACAAAACAGCAAGTGTTGTAATGTCTCAGGCTTTTGGCGTTC
CGGCATTTCCTGTAGATACACATATTCATAGATTGATGCACAGATGGAATTTGTCGAACGGAAAAAGTGTAGCTCAGACT
GAAAAAGATGCTAAACGATTATTCCCAAGAGATTTATGGAACGATTTGCATTTACAGATCATTTGGTATGGCCGAGAATA
TTCTCCTGCCCGTGGATGGAGTCTTGAAAAAGATATTATTACCAGAACTGTCGGCAAAAAATCAATATTAGAAGAAGCTG
CTAAAAAGAAAGTCTAG

Upstream 100 bases:

>100_bases
TCCTGATGAATCTACCCATTGTAAATTACCCATATTTTATATTATTACTAAACTGCTCAAGTAAAATTTATATTACTTTT
ACGACTTTAAAATTACGGAA

Downstream 100 bases:

>100_bases
CAGCTTCTGTTTTTTTTACATCCCGTTTTTTAACGCATTGTATTCATTTGTCATATCAAGTGATCGATATACGCCTAAAA
GTGTTTTTGTAGCTTCTTCA

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MNKEARVQFVINTLKELYPTIPVPLDHKDPYTLLIAVLLSAQCTDVRVNQITPLLFAKADNPYDMVKMSIEEIKEIIRPC
GLSPMKSKGIHGLSEILIEKHNGEVPQSFEALEELPAVGHKTASVVMSQAFGVPAFPVDTHIHRLMHRWNLSNGKSVAQT
EKDAKRLFPRDLWNDLHLQIIWYGREYSPARGWSLEKDIITRTVGKKSILEEAAKKKV

Sequences:

>Translated_218_residues
MNKEARVQFVINTLKELYPTIPVPLDHKDPYTLLIAVLLSAQCTDVRVNQITPLLFAKADNPYDMVKMSIEEIKEIIRPC
GLSPMKSKGIHGLSEILIEKHNGEVPQSFEALEELPAVGHKTASVVMSQAFGVPAFPVDTHIHRLMHRWNLSNGKSVAQT
EKDAKRLFPRDLWNDLHLQIIWYGREYSPARGWSLEKDIITRTVGKKSILEEAAKKKV
>Mature_218_residues
MNKEARVQFVINTLKELYPTIPVPLDHKDPYTLLIAVLLSAQCTDVRVNQITPLLFAKADNPYDMVKMSIEEIKEIIRPC
GLSPMKSKGIHGLSEILIEKHNGEVPQSFEALEELPAVGHKTASVVMSQAFGVPAFPVDTHIHRLMHRWNLSNGKSVAQT
EKDAKRLFPRDLWNDLHLQIIWYGREYSPARGWSLEKDIITRTVGKKSILEEAAKKKV

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=159, Percent_Identity=29.559748427673, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1787920, Length=191, Percent_Identity=42.4083769633508, Blast_Score=146, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17554540, Length=163, Percent_Identity=25.7668711656442, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=24.5989304812834, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=27.1186440677966, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24715; Mature: 24715

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEARVQFVINTLKELYPTIPVPLDHKDPYTLLIAVLLSAQCTDVRVNQITPLLFAKAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHCEEEEECCC
NPYDMVKMSIEEIKEIIRPCGLSPMKSKGIHGLSEILIEKHNGEVPQSFEALEELPAVGH
CHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
KTASVVMSQAFGVPAFPVDTHIHRLMHRWNLSNGKSVAQTEKDAKRLFPRDLWNDLHLQI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHCCCEEEE
IWYGREYSPARGWSLEKDIITRTVGKKSILEEAAKKKV
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKEARVQFVINTLKELYPTIPVPLDHKDPYTLLIAVLLSAQCTDVRVNQITPLLFAKAD
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHCEEEEECCC
NPYDMVKMSIEEIKEIIRPCGLSPMKSKGIHGLSEILIEKHNGEVPQSFEALEELPAVGH
CHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
KTASVVMSQAFGVPAFPVDTHIHRLMHRWNLSNGKSVAQTEKDAKRLFPRDLWNDLHLQI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHCCCEEEE
IWYGREYSPARGWSLEKDIITRTVGKKSILEEAAKKKV
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]