Click here to switch to the map view.

The map label for this gene is insK [C]

Identifier: 146284646

GI number: 146284646

Start: 151435

End: 152229

Strand: Reverse

Name: insK [C]

Synonym: Ent638_4320

Alternate gene names: 146284646

Gene position: 152229-151435 (Counterclockwise)

Preceding gene: 146284647

Following gene: 146284641

Centisome position: 96.5

GC content: 51.57

Gene sequence:

>795_bases
ATGAGTTTACGCCAGGCCTGCAGGACGTTATCGCTGAGCAGGACGGTATATTTTTATCAGCCTGATACCCGGCGTGATGA
ACCGGTGATCCAGGCGCTGACTGAACTGGCAGAACGCTATCCGCGCTACGGTTTTAAGAAGCTTTTTCAGGTGCTTCGCA
GGCACGGTAATGCCTGGAACCATAAACGCGTTCACCGGATTTACTGCCTGCTGAAACTGAATTTTCGTCGTAAGGGTAAA
CAACGTCTGCCGGTGCGCAACCCGACCCCGCTGGCAACACCGCAAGCGTTAAACCAGAGCTGGTCGATTGATTTTATGCA
CGATGCACTCGTCTGCGGCAGACGCTTTCGAACCTTCAACGTGGTGGATGATTTTAACCGTGAGGTGCTGGCGATAGAAA
TCGACCTGAATATTCCAGCTCAGCGTGTCGTCCGGGTACTGGACAGGATAGTAGCAAACCGCGGATATCCTCTGAAGATG
CGGATGGATAACGGGCCAGAACTGGTCTCACTGGCACTGGCGCAGTGGGCGGAAGAACACGGGGTGATGCTGGACTTTAT
CAAACCGGGTAAGCCGACCCAGAATGCCTTTATCGAACGGTTTAACCGGACGTACCGGACAGAAATACTGGATTTTTACC
TGTTCAGAACCCTGAATGAAGCACGGGAAATTACAGAGCGCTGGCTGGCTGAATATAACAGTGAGCGACCTCATGAATCC
CTGAATAACCTGACGCCGGAAGAATACAGATTGATGGCCGAAAACACGGAAATCTCAAAAAGTGCGTGGAACTAA

Upstream 100 bases:

>100_bases
TCAGTCTGGAATGCCGGGCGTTGAAAGATGTTATCGAAAAAAAGCTTTAAAACCAGCGATAAAGCGTGAACTCGTCCGCT
ATCTGACGGCGCATTTTGCC

Downstream 100 bases:

>100_bases
AACGGGTGTGCTTACAGCTCCGGCATCAGCCAGACGAGGAGAGAACTGATAGCCCAGCAACCAGAAAAGACCGAAGATCA
GGTCACTGGAACCTGCCGTA

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSLRQACRTLSLSRTVYFYQPDTRRDEPVIQALTELAERYPRYGFKKLFQVLRRHGNAWNHKRVHRIYCLLKLNFRRKGK
QRLPVRNPTPLATPQALNQSWSIDFMHDALVCGRRFRTFNVVDDFNREVLAIEIDLNIPAQRVVRVLDRIVANRGYPLKM
RMDNGPELVSLALAQWAEEHGVMLDFIKPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEAREITERWLAEYNSERPHES
LNNLTPEEYRLMAENTEISKSAWN

Sequences:

>Translated_264_residues
MSLRQACRTLSLSRTVYFYQPDTRRDEPVIQALTELAERYPRYGFKKLFQVLRRHGNAWNHKRVHRIYCLLKLNFRRKGK
QRLPVRNPTPLATPQALNQSWSIDFMHDALVCGRRFRTFNVVDDFNREVLAIEIDLNIPAQRVVRVLDRIVANRGYPLKM
RMDNGPELVSLALAQWAEEHGVMLDFIKPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEAREITERWLAEYNSERPHES
LNNLTPEEYRLMAENTEISKSAWN
>Mature_263_residues
SLRQACRTLSLSRTVYFYQPDTRRDEPVIQALTELAERYPRYGFKKLFQVLRRHGNAWNHKRVHRIYCLLKLNFRRKGKQ
RLPVRNPTPLATPQALNQSWSIDFMHDALVCGRRFRTFNVVDDFNREVLAIEIDLNIPAQRVVRVLDRIVANRGYPLKMR
MDNGPELVSLALAQWAEEHGVMLDFIKPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEAREITERWLAEYNSERPHESL
NNLTPEEYRLMAENTEISKSAWN

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=267, Percent_Identity=26.2172284644195, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786462, Length=239, Percent_Identity=26.7782426778243, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31385; Mature: 31254

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRQACRTLSLSRTVYFYQPDTRRDEPVIQALTELAERYPRYGFKKLFQVLRRHGNAWN
CCHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
HKRVHRIYCLLKLNFRRKGKQRLPVRNPTPLATPQALNQSWSIDFMHDALVCGRRFRTFN
HHHHHHHHHHEEECHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEC
VVDDFNREVLAIEIDLNIPAQRVVRVLDRIVANRGYPLKMRMDNGPELVSLALAQWAEEH
HHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHC
GVMLDFIKPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEAREITERWLAEYNSERPHES
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LNNLTPEEYRLMAENTEISKSAWN
HHCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SLRQACRTLSLSRTVYFYQPDTRRDEPVIQALTELAERYPRYGFKKLFQVLRRHGNAWN
CHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCC
HKRVHRIYCLLKLNFRRKGKQRLPVRNPTPLATPQALNQSWSIDFMHDALVCGRRFRTFN
HHHHHHHHHHEEECHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEC
VVDDFNREVLAIEIDLNIPAQRVVRVLDRIVANRGYPLKMRMDNGPELVSLALAQWAEEH
HHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHC
GVMLDFIKPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEAREITERWLAEYNSERPHES
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
LNNLTPEEYRLMAENTEISKSAWN
HHCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]