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The map label for this gene is repA [H]

Identifier: 146284575

GI number: 146284575

Start: 77908

End: 78768

Strand: Direct

Name: repA [H]

Synonym: Ent638_4249

Alternate gene names: 146284575

Gene position: 77908-78768 (Clockwise)

Preceding gene: 146284573

Following gene: 146284576

Centisome position: 49.39

GC content: 41.81

Gene sequence:

>861_bases
ATGACTGATAACAATTTTATACCAGCTCAGATTACTAATGAAGGCGAAACTCATCAATTAACCGTCACAAGCAATTCATC
TGTCCAGCCGGCTATTTTGTTGCGTCTTGGGGTCTTTGTTCCTGCTAGTAGAGCAATACCTAAAGGGCTGAAGTCAGGTA
TAGATGTATCATCCTCATTCTCACAGTTGGAGTTTGCTCGTAAGGAAGGTTACGACGAAGTCAGGATCTTTGGAGAAAGG
CTAAACATATCAACAGATTTTAGTGTCTGGATGGGTATTATCGGCGCTTTTAGCAAATACGGTTTGAAATCAAATACGAT
AGAACTGCCTTTTACTGAGTTTGCTGCGTTGTGCCAGTACGATTCGCGTCAGTTTAACAAACGTTTGAGAGACACGATCT
TTGATGCACTGACCCGCATCGGTACTAAAACGGTTCAGTTTAATAGCAAGAAAACAGGCGACAAGTTCTTCACTCAACTT
CTCAAAAGTGGCAAGTACACAAAAGATACTGACACCATCACACTTCAAGCTGATGAGCGACTGCACGAACTCTATCAAAT
TGACTATAACATCCTGTTGCGTAAGCGTCCTTATCATATGCTGAAAGGCAAGGAAGTCGCTCAAACCCTTTATACCTACA
TTGCGAGCCTGCCCGATAACCCTGCCCCGATCAGATTTGATCGCATCATTGAACGACTTAACTTAACTTCTCCAGTTAAA
GAACAAAACCGGCTCATTAAGGCGGCTTTGAAGCAATTGCATGACATTGGCTATATCGAATATAGCATTGTGAAAGATGG
CCGGACTTTGACCGTGTTAATTCACAAACGCAATAAGAAACTTAAGGAATTACAACCTTAA

Upstream 100 bases:

>100_bases
GTCATAACATGTTTTGTGGTGTGTCGTAGCATGTATAATTACTAACTATTCACCTCTTGTGGATAACCCTGAATGGACTC
AACATAACTGGCGTAACTCA

Downstream 100 bases:

>100_bases
AAGGTGGGTCGTATTATGCTTACGATCCAGCAATAGTGGGTCGTAGCATGTGGTGAGGTGTGCTACAACATGATGTCTTC
ACTCAGTAACTTTCTGCACC

Product: initiator RepB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTDNNFIPAQITNEGETHQLTVTSNSSVQPAILLRLGVFVPASRAIPKGLKSGIDVSSSFSQLEFARKEGYDEVRIFGER
LNISTDFSVWMGIIGAFSKYGLKSNTIELPFTEFAALCQYDSRQFNKRLRDTIFDALTRIGTKTVQFNSKKTGDKFFTQL
LKSGKYTKDTDTITLQADERLHELYQIDYNILLRKRPYHMLKGKEVAQTLYTYIASLPDNPAPIRFDRIIERLNLTSPVK
EQNRLIKAALKQLHDIGYIEYSIVKDGRTLTVLIHKRNKKLKELQP

Sequences:

>Translated_286_residues
MTDNNFIPAQITNEGETHQLTVTSNSSVQPAILLRLGVFVPASRAIPKGLKSGIDVSSSFSQLEFARKEGYDEVRIFGER
LNISTDFSVWMGIIGAFSKYGLKSNTIELPFTEFAALCQYDSRQFNKRLRDTIFDALTRIGTKTVQFNSKKTGDKFFTQL
LKSGKYTKDTDTITLQADERLHELYQIDYNILLRKRPYHMLKGKEVAQTLYTYIASLPDNPAPIRFDRIIERLNLTSPVK
EQNRLIKAALKQLHDIGYIEYSIVKDGRTLTVLIHKRNKKLKELQP
>Mature_285_residues
TDNNFIPAQITNEGETHQLTVTSNSSVQPAILLRLGVFVPASRAIPKGLKSGIDVSSSFSQLEFARKEGYDEVRIFGERL
NISTDFSVWMGIIGAFSKYGLKSNTIELPFTEFAALCQYDSRQFNKRLRDTIFDALTRIGTKTVQFNSKKTGDKFFTQLL
KSGKYTKDTDTITLQADERLHELYQIDYNILLRKRPYHMLKGKEVAQTLYTYIASLPDNPAPIRFDRIIERLNLTSPVKE
QNRLIKAALKQLHDIGYIEYSIVKDGRTLTVLIHKRNKKLKELQP

Specific function: RepA is essential for origin function, autoregulates its own synthesis from the promoter, and, when overproduced, blocks origin function [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the initiator repB protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000525 [H]

Pfam domain/function: PF01051 Rep_3 [H]

EC number: NA

Molecular weight: Translated: 32716; Mature: 32585

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNNFIPAQITNEGETHQLTVTSNSSVQPAILLRLGVFVPASRAIPKGLKSGIDVSSSF
CCCCCCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHH
SQLEFARKEGYDEVRIFGERLNISTDFSVWMGIIGAFSKYGLKSNTIELPFTEFAALCQY
HHHHHHHHCCCHHHEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHH
DSRQFNKRLRDTIFDALTRIGTKTVQFNSKKTGDKFFTQLLKSGKYTKDTDTITLQADER
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEECHHH
LHELYQIDYNILLRKRPYHMLKGKEVAQTLYTYIASLPDNPAPIRFDRIIERLNLTSPVK
HHHHHHHCHHEEEECCCHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHH
EQNRLIKAALKQLHDIGYIEYSIVKDGRTLTVLIHKRNKKLKELQP
HHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCHHCCCC
>Mature Secondary Structure 
TDNNFIPAQITNEGETHQLTVTSNSSVQPAILLRLGVFVPASRAIPKGLKSGIDVSSSF
CCCCCCCEEECCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHH
SQLEFARKEGYDEVRIFGERLNISTDFSVWMGIIGAFSKYGLKSNTIELPFTEFAALCQY
HHHHHHHHCCCHHHEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHH
DSRQFNKRLRDTIFDALTRIGTKTVQFNSKKTGDKFFTQLLKSGKYTKDTDTITLQADER
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEECHHH
LHELYQIDYNILLRKRPYHMLKGKEVAQTLYTYIASLPDNPAPIRFDRIIERLNLTSPVK
HHHHHHHCHHEEEECCCHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHH
EQNRLIKAALKQLHDIGYIEYSIVKDGRTLTVLIHKRNKKLKELQP
HHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3949778; 2198259 [H]