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The map label for this gene is tnpR [H]

Identifier: 146284549

GI number: 146284549

Start: 33460

End: 34044

Strand: Reverse

Name: tnpR [H]

Synonym: Ent638_4223

Alternate gene names: 146284549

Gene position: 34044-33460 (Counterclockwise)

Preceding gene: 146284551

Following gene: 146284545

Centisome position: 21.58

GC content: 50.77

Gene sequence:

>585_bases
ATGTCACGTGTTTTTGCCTACTGTCGTGTCTCTACCCTGGAGCAGACAACCGAAAATCAGCGAAGAGAAATTGAGACGGC
GGGTTTCGCTGTTAAATCTCAGCGACTGATTGAAGAACACATCAGCGGCTCAGTTGCCGCCAGTGAGCGGCCAGGTTTTT
CTAAACTGCTCGACCGGATGCAAAATGGCGATGTGCTGATTGTTACCAAACTAGACCGACTGGGGCGCAATGCGATGGAT
ATCAGAAAAACGGTGGAACAGCTGGCTGCTTCAGATATTCGCGTTCACTGTCTCGCTCTGGGCGGAGTGGATCTGACCAG
CCCGGCAGGAAGAATGACCATGCAGGTCATCTCTGCTGTGGCTGAATTTGAAAGAGATTTGCTGCTTGAGCGTACGCACT
CTGGTATTGCGCGAGCAAAAGCAGCCGGAAAGCGGTTTGGACGTCCTTCAGTACTGAATGAAGAACAGCAACTTACGGTG
ATTGTACGGATTAACGCCGGGATCAGTATCAGCACCATTGCGCGGGAATTTAATACCACCCGACAAACCATACTTCGGGT
AAAGTCAGGGCATCAACAGTCTTGA

Upstream 100 bases:

>100_bases
GGTTTCGTACACCTATCATCAATGCTGTCCGAAAGTCAGTGAATTTACTATCGTACATGCGTTATAATTCGTACATCCTT
TTCGTACAGAAAATATCACT

Downstream 100 bases:

>100_bases
ATATATACCTCTATCGACTGTGAAGGCAGGGACCGCATAAGGAATAGGATTTATGCGATCCGCATATTTTTTCTTACATC
ATTTGAACAGTAGGTCTGAT

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MSRVFAYCRVSTLEQTTENQRREIETAGFAVKSQRLIEEHISGSVAASERPGFSKLLDRMQNGDVLIVTKLDRLGRNAMD
IRKTVEQLAASDIRVHCLALGGVDLTSPAGRMTMQVISAVAEFERDLLLERTHSGIARAKAAGKRFGRPSVLNEEQQLTV
IVRINAGISISTIAREFNTTRQTILRVKSGHQQS

Sequences:

>Translated_194_residues
MSRVFAYCRVSTLEQTTENQRREIETAGFAVKSQRLIEEHISGSVAASERPGFSKLLDRMQNGDVLIVTKLDRLGRNAMD
IRKTVEQLAASDIRVHCLALGGVDLTSPAGRMTMQVISAVAEFERDLLLERTHSGIARAKAAGKRFGRPSVLNEEQQLTV
IVRINAGISISTIAREFNTTRQTILRVKSGHQQS
>Mature_193_residues
SRVFAYCRVSTLEQTTENQRREIETAGFAVKSQRLIEEHISGSVAASERPGFSKLLDRMQNGDVLIVTKLDRLGRNAMDI
RKTVEQLAASDIRVHCLALGGVDLTSPAGRMTMQVISAVAEFERDLLLERTHSGIARAKAAGKRFGRPSVLNEEQQLTVI
VRINAGISISTIAREFNTTRQTILRVKSGHQQS

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787638, Length=192, Percent_Identity=76.0416666666667, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1787827, Length=192, Percent_Identity=75.5208333333333, Blast_Score=303, Evalue=5e-84,
Organism=Escherichia coli, GI1787404, Length=185, Percent_Identity=36.7567567567568, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21476; Mature: 21345

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVFAYCRVSTLEQTTENQRREIETAGFAVKSQRLIEEHISGSVAASERPGFSKLLDRM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QNGDVLIVTKLDRLGRNAMDIRKTVEQLAASDIRVHCLALGGVDLTSPAGRMTMQVISAV
CCCCEEEEECHHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
AEFERDLLLERTHSGIARAKAAGKRFGRPSVLNEEQQLTVIVRINAGISISTIAREFNTT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
RQTILRVKSGHQQS
HHHHHHHHCCCCCC
>Mature Secondary Structure 
SRVFAYCRVSTLEQTTENQRREIETAGFAVKSQRLIEEHISGSVAASERPGFSKLLDRM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QNGDVLIVTKLDRLGRNAMDIRKTVEQLAASDIRVHCLALGGVDLTSPAGRMTMQVISAV
CCCCEEEEECHHHHCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
AEFERDLLLERTHSGIARAKAAGKRFGRPSVLNEEQQLTVIVRINAGISISTIAREFNTT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
RQTILRVKSGHQQS
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3027041 [H]