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The map label for this gene is rpfC [H]

Identifier: 146284529

GI number: 146284529

Start: 6810

End: 8372

Strand: Reverse

Name: rpfC [H]

Synonym: Ent638_4203

Alternate gene names: 146284529

Gene position: 8372-6810 (Counterclockwise)

Preceding gene: 146284530

Following gene: NA

Centisome position: 5.31

GC content: 41.01

Gene sequence:

>1563_bases
ATGGCACTAAGTTATGTCAGTATGATTGCTCTATTCTTTGGCGAATACACTTGGGATTATCTGTTTATTGCATTGTACTA
TTTGGGTTTTCTTTTAACATCAATCCTTTTAAGGTTCATCATAAAGCGTAATCCTGGTATTTTCCTTGTACGCCGTATGT
TTACCATGATTCACGATTATGTGGCCATTTCGGTTGGCCTGGCTGTAGGAGAAGAAAAGACACTCCCAATATACGCTGTA
ATGGTATGGGTGACATTGGGTTATGGCGTACGTTATGGTGCTTTCTATCTCCTTGCTGCGACAATCATGTCGTTCATTTC
TTTATCAGTAATAGTGTTCTACAACGATTACTGGTCTAACCATCTCTATATGGTACTGGCCTTATTGCTGACCACTGTCG
TTATCCCAGTATACGCTAAAACACTTCTTACACAGGTACGCCAGGCGTCCACCCGAGCCCTTAAGGCAACACACACAAAA
GCCCAGCTACTAGCCCAAGTCAGCCACGATCTTCGGCAACCCATTCACGCCATCGGAATCTATATTACCTGCCTCCGAGA
AGAAAATTTAAACCCCAGCGGGCTTAAAATGGTCGATAACATCGATCGTTCTCTGGTTAGTGTGTCACATATATTTCATT
CAATGCTCAATTTTTACACCCTGGATAGCGGGGGGATCAATCTGAAACCTGAAGTGTTCAGATTACGTAATATGCTTGAA
GAATGCATTAAGGAACATACTGTTGCCGCACATGAAGCGGGAAGTCAGATACGACTGGATGTAGAAGAGTGTTGGGTCAA
ATCTGATGTCTCCATACTTATAATAATTATCAATAATCTGATCAGTAATGCCGTTAAATATGGGGAAGGTAAACTGATAT
CTCTGCGTAGTTTCCATAAGAATGGAAGTATAATGCTAGCTATATGTGATAAAGGTTCAGGCATAGAAGAGAAACATCTT
CTGCATATTTTTGATGAGTTTTTCCGCATCAAAAAAAACAGAGATAAAGATCTTGAGGGTATTGGTCTTGGTTTGTCCAT
AGTAAAACGTGCCTGCTCCCTTGCAGGTTTAGATATCAATCTTGTCTCCCGTGTTGGTCTAGGGACCTGTGCTGTTATTT
CAAATCTAAAGATTGCCAGTACCCCAGTTGTCCAAGATAAACCGCAAGAAAAACTACTCATGCAGCTTTCCGGGACTCGG
GTGTTTCTTATTGAGGATAACCCCGAAATGCTGGATGCCACACAGATGCTACTTCAGCGTTGGGGATGTGAAGTCCTACC
ACTAGAATCACAGGATCCAACACTACTGGATACCCAATGTGAATGCGATGTTATCATTGCGGATTATGATCTGGGCACAA
AAATTTCAGGTATTGAATATATACAGGCAATTCGAAATATGAAAGCAGAACAAATCCCTGCACTGCTCATCACAGGCCAT
GATGTCACTAATATTAGTATCTTTGTTAAGGATCTCAATATTGAGATAGTTTCCAAGCCTATTAAACCCGTGGAACTAAG
AAGTGTACTAACGGATATCGTAAGAAAAACAAAAGCGATTTAA

Upstream 100 bases:

>100_bases
ATCCTCACTGGTGAAGGCAATGCCATTTAGAGAATATGAAATTCAAAGCCAGCACATTTCAGAATTAAACCAGGCAACAT
TGCGGGTGGTCATCGCCATA

Downstream 100 bases:

>100_bases
ACTACCAGAAATAGGCATCCATTTTCTCAAATATATTTTAAATCTGGATGTAACTTCTTGGATTCACATGGTTTTCCATC
CACAAATCATTTATTTTGCA

Product: integral membrane sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MALSYVSMIALFFGEYTWDYLFIALYYLGFLLTSILLRFIIKRNPGIFLVRRMFTMIHDYVAISVGLAVGEEKTLPIYAV
MVWVTLGYGVRYGAFYLLAATIMSFISLSVIVFYNDYWSNHLYMVLALLLTTVVIPVYAKTLLTQVRQASTRALKATHTK
AQLLAQVSHDLRQPIHAIGIYITCLREENLNPSGLKMVDNIDRSLVSVSHIFHSMLNFYTLDSGGINLKPEVFRLRNMLE
ECIKEHTVAAHEAGSQIRLDVEECWVKSDVSILIIIINNLISNAVKYGEGKLISLRSFHKNGSIMLAICDKGSGIEEKHL
LHIFDEFFRIKKNRDKDLEGIGLGLSIVKRACSLAGLDINLVSRVGLGTCAVISNLKIASTPVVQDKPQEKLLMQLSGTR
VFLIEDNPEMLDATQMLLQRWGCEVLPLESQDPTLLDTQCECDVIIADYDLGTKISGIEYIQAIRNMKAEQIPALLITGH
DVTNISIFVKDLNIEIVSKPIKPVELRSVLTDIVRKTKAI

Sequences:

>Translated_520_residues
MALSYVSMIALFFGEYTWDYLFIALYYLGFLLTSILLRFIIKRNPGIFLVRRMFTMIHDYVAISVGLAVGEEKTLPIYAV
MVWVTLGYGVRYGAFYLLAATIMSFISLSVIVFYNDYWSNHLYMVLALLLTTVVIPVYAKTLLTQVRQASTRALKATHTK
AQLLAQVSHDLRQPIHAIGIYITCLREENLNPSGLKMVDNIDRSLVSVSHIFHSMLNFYTLDSGGINLKPEVFRLRNMLE
ECIKEHTVAAHEAGSQIRLDVEECWVKSDVSILIIIINNLISNAVKYGEGKLISLRSFHKNGSIMLAICDKGSGIEEKHL
LHIFDEFFRIKKNRDKDLEGIGLGLSIVKRACSLAGLDINLVSRVGLGTCAVISNLKIASTPVVQDKPQEKLLMQLSGTR
VFLIEDNPEMLDATQMLLQRWGCEVLPLESQDPTLLDTQCECDVIIADYDLGTKISGIEYIQAIRNMKAEQIPALLITGH
DVTNISIFVKDLNIEIVSKPIKPVELRSVLTDIVRKTKAI
>Mature_519_residues
ALSYVSMIALFFGEYTWDYLFIALYYLGFLLTSILLRFIIKRNPGIFLVRRMFTMIHDYVAISVGLAVGEEKTLPIYAVM
VWVTLGYGVRYGAFYLLAATIMSFISLSVIVFYNDYWSNHLYMVLALLLTTVVIPVYAKTLLTQVRQASTRALKATHTKA
QLLAQVSHDLRQPIHAIGIYITCLREENLNPSGLKMVDNIDRSLVSVSHIFHSMLNFYTLDSGGINLKPEVFRLRNMLEE
CIKEHTVAAHEAGSQIRLDVEECWVKSDVSILIIIINNLISNAVKYGEGKLISLRSFHKNGSIMLAICDKGSGIEEKHLL
HIFDEFFRIKKNRDKDLEGIGLGLSIVKRACSLAGLDINLVSRVGLGTCAVISNLKIASTPVVQDKPQEKLLMQLSGTRV
FLIEDNPEMLDATQMLLQRWGCEVLPLESQDPTLLDTQCECDVIIADYDLGTKISGIEYIQAIRNMKAEQIPALLITGHD
VTNISIFVKDLNIEIVSKPIKPVELRSVLTDIVRKTKAI

Specific function: Involved in the positive regulation of synthesis of extracellular enzymes and polysaccharide, and hence in pathogenicity. Can restore function to the non-pathogenic mutant 8237 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI87081816, Length=353, Percent_Identity=27.4787535410765, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI145693157, Length=327, Percent_Identity=26.605504587156, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789149, Length=235, Percent_Identity=29.7872340425532, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994928, Length=259, Percent_Identity=25.0965250965251, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=27.0161290322581, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=27.0386266094421, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786600, Length=239, Percent_Identity=25.9414225941423, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1788549, Length=236, Percent_Identity=26.271186440678, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082128, Length=215, Percent_Identity=24.1860465116279, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786783, Length=227, Percent_Identity=22.4669603524229, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58437; Mature: 58305

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSYVSMIALFFGEYTWDYLFIALYYLGFLLTSILLRFIIKRNPGIFLVRRMFTMIHDY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VAISVGLAVGEEKTLPIYAVMVWVTLGYGVRYGAFYLLAATIMSFISLSVIVFYNDYWSN
HHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEECHHCC
HLYMVLALLLTTVVIPVYAKTLLTQVRQASTRALKATHTKAQLLAQVSHDLRQPIHAIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YITCLREENLNPSGLKMVDNIDRSLVSVSHIFHSMLNFYTLDSGGINLKPEVFRLRNMLE
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHH
ECIKEHTVAAHEAGSQIRLDVEECWVKSDVSILIIIINNLISNAVKYGEGKLISLRSFHK
HHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEECC
NGSIMLAICDKGSGIEEKHLLHIFDEFFRIKKNRDKDLEGIGLGLSIVKRACSLAGLDIN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
LVSRVGLGTCAVISNLKIASTPVVQDKPQEKLLMQLSGTRVFLIEDNPEMLDATQMLLQR
HHHHCCCCHHHHHCCCEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
WGCEVLPLESQDPTLLDTQCECDVIIADYDLGTKISGIEYIQAIRNMKAEQIPALLITGH
CCCCEEECCCCCCCEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHCCEEEEECC
DVTNISIFVKDLNIEIVSKPIKPVELRSVLTDIVRKTKAI
CCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALSYVSMIALFFGEYTWDYLFIALYYLGFLLTSILLRFIIKRNPGIFLVRRMFTMIHDY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VAISVGLAVGEEKTLPIYAVMVWVTLGYGVRYGAFYLLAATIMSFISLSVIVFYNDYWSN
HHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEECHHCC
HLYMVLALLLTTVVIPVYAKTLLTQVRQASTRALKATHTKAQLLAQVSHDLRQPIHAIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YITCLREENLNPSGLKMVDNIDRSLVSVSHIFHSMLNFYTLDSGGINLKPEVFRLRNMLE
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHH
ECIKEHTVAAHEAGSQIRLDVEECWVKSDVSILIIIINNLISNAVKYGEGKLISLRSFHK
HHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEECC
NGSIMLAICDKGSGIEEKHLLHIFDEFFRIKKNRDKDLEGIGLGLSIVKRACSLAGLDIN
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
LVSRVGLGTCAVISNLKIASTPVVQDKPQEKLLMQLSGTRVFLIEDNPEMLDATQMLLQR
HHHHCCCCHHHHHCCCEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHH
WGCEVLPLESQDPTLLDTQCECDVIIADYDLGTKISGIEYIQAIRNMKAEQIPALLITGH
CCCCEEECCCCCCCEECCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHCCEEEEECC
DVTNISIFVKDLNIEIVSKPIKPVELRSVLTDIVRKTKAI
CCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1645442 [H]