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The map label for this gene is leuB [H]

Identifier: 145300083

GI number: 145300083

Start: 3446902

End: 3447909

Strand: Direct

Name: leuB [H]

Synonym: ASA_3183

Alternate gene names: 145300083

Gene position: 3446902-3447909 (Clockwise)

Preceding gene: 145300082

Following gene: 145300084

Centisome position: 73.3

GC content: 59.42

Gene sequence:

>1008_bases
ATGTCAAAAAGAAAGATTACCGTCATCCCGGGCGACGGCATTGGCCCCAGCATCATCGAATCAGCCATCCAGATCCTGAC
CCACGCCGGTTGTGACTTCGAATACGAGTATGCGGATGCCGGTCTGGTCGCGCTGGAGAAGCACGGCGAGCTGCTGCCCC
AGGCAACCCTGGATCTGATCGAGAAGAACAAGGTCAGCCTGAAAGGCCCGCTCACCACCCCGGTTGGCGGCGGCTTCACC
TCCATCAACGTCTCCCTGCGCAAAAAATTCAACCTCTACGCCAACGTCCGCCCCGTCATCTCCTTCAAGGGTACCAAGAG
TCGTTACGAGAACATCGATATCATCACAGTGCGCGAGAACACCGAAGGGATGTACTCGGGCGCCGGCCAGAAGCGCAGCG
ATGACAACAATAGCGCCGAGGCGATGAGCATCGTTACCCGCGACGGGGCAGAACGCATCGTCAAGTTTGCCTTCGAACTG
GCCCGCCAGGAAGGACGCAAGAAAGTGACCATCATCCACAAGGCCAACATTCTCAAGTCCACCTCTGGCCTGTTTCTGGA
AGTGGCCCGCGATGTCGCCGCTCGCTACCCGGATATCCAGAGCGAAGAGATGATTGTCGATGCCGCCTGCATGAACCTGG
TGATGTACCCGGAGCGGTTCGATGTCATGGTCACCACCAACCTGTTTGGCGACATCCTCTCGGATCTGTGCGCCGGTCTG
GTGGGCGGTCTGGGCATGGCACCTGGTGCCAACATCGGCGATGGAGCCGCCATCTTCGAAGCTGTGCATGGCTCGGCGCC
GGATATCGCAGGCAAGAACATCGCCAACCCGACCTCGGTGATCCTCGCCGCCATCCAGATGCTGGAATACCTGGGCATGC
AGGACAAGGCTGAGCGCATCCGTGAGGCTGTGCGCGCCACCATCGAATCCGGTGATCGCGTGACCCGGGATCTCGGGGGG
ACCGCCTCTACCAGCGAGTTCACCCAATCCATCATAGACCGTTTGTAA

Upstream 100 bases:

>100_bases
AAAAACAGATAGAATGTAAAGAAACATGGAGGGTTAGTTGTATAAATGGTATAAAACGGCCGAATCCTCCCCCTCTGTTC
CCTAGAAGTGGATACCTCTC

Downstream 100 bases:

>100_bases
TTCATCTTAATTCATTGATTCAAAAGGGGAGCTCAGGCTCCCCTTGTTTTATCTCGCGGCACGCTACCCGCCTAGTCAAT
TGGCTACTATGTTCAATGCA

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSKRKITVIPGDGIGPSIIESAIQILTHAGCDFEYEYADAGLVALEKHGELLPQATLDLIEKNKVSLKGPLTTPVGGGFT
SINVSLRKKFNLYANVRPVISFKGTKSRYENIDIITVRENTEGMYSGAGQKRSDDNNSAEAMSIVTRDGAERIVKFAFEL
ARQEGRKKVTIIHKANILKSTSGLFLEVARDVAARYPDIQSEEMIVDAACMNLVMYPERFDVMVTTNLFGDILSDLCAGL
VGGLGMAPGANIGDGAAIFEAVHGSAPDIAGKNIANPTSVILAAIQMLEYLGMQDKAERIREAVRATIESGDRVTRDLGG
TASTSEFTQSIIDRL

Sequences:

>Translated_335_residues
MSKRKITVIPGDGIGPSIIESAIQILTHAGCDFEYEYADAGLVALEKHGELLPQATLDLIEKNKVSLKGPLTTPVGGGFT
SINVSLRKKFNLYANVRPVISFKGTKSRYENIDIITVRENTEGMYSGAGQKRSDDNNSAEAMSIVTRDGAERIVKFAFEL
ARQEGRKKVTIIHKANILKSTSGLFLEVARDVAARYPDIQSEEMIVDAACMNLVMYPERFDVMVTTNLFGDILSDLCAGL
VGGLGMAPGANIGDGAAIFEAVHGSAPDIAGKNIANPTSVILAAIQMLEYLGMQDKAERIREAVRATIESGDRVTRDLGG
TASTSEFTQSIIDRL
>Mature_334_residues
SKRKITVIPGDGIGPSIIESAIQILTHAGCDFEYEYADAGLVALEKHGELLPQATLDLIEKNKVSLKGPLTTPVGGGFTS
INVSLRKKFNLYANVRPVISFKGTKSRYENIDIITVRENTEGMYSGAGQKRSDDNNSAEAMSIVTRDGAERIVKFAFELA
RQEGRKKVTIIHKANILKSTSGLFLEVARDVAARYPDIQSEEMIVDAACMNLVMYPERFDVMVTTNLFGDILSDLCAGLV
GGLGMAPGANIGDGAAIFEAVHGSAPDIAGKNIANPTSVILAAIQMLEYLGMQDKAERIREAVRATIESGDRVTRDLGGT
ASTSEFTQSIIDRL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=333, Percent_Identity=46.8468468468468, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI4758582, Length=336, Percent_Identity=42.5595238095238, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI28178821, Length=338, Percent_Identity=40.5325443786982, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI28178838, Length=311, Percent_Identity=43.0868167202572, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI28178816, Length=338, Percent_Identity=40.2366863905325, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI28178819, Length=198, Percent_Identity=46.4646464646465, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1788101, Length=357, Percent_Identity=35.8543417366947, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081683, Length=360, Percent_Identity=37.5, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1787381, Length=393, Percent_Identity=33.3333333333333, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71986051, Length=332, Percent_Identity=47.5903614457831, Blast_Score=328, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI25144293, Length=338, Percent_Identity=42.8994082840237, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI17550882, Length=339, Percent_Identity=40.7079646017699, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17505779, Length=337, Percent_Identity=40.9495548961424, Blast_Score=237, Evalue=8e-63,
Organism=Saccharomyces cerevisiae, GI6324709, Length=337, Percent_Identity=44.213649851632, Blast_Score=293, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6324291, Length=335, Percent_Identity=41.1940298507463, Blast_Score=249, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6322097, Length=348, Percent_Identity=38.2183908045977, Blast_Score=215, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6319830, Length=361, Percent_Identity=29.6398891966759, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24643270, Length=332, Percent_Identity=47.5903614457831, Blast_Score=340, Evalue=9e-94,
Organism=Drosophila melanogaster, GI24643268, Length=332, Percent_Identity=47.5903614457831, Blast_Score=340, Evalue=9e-94,
Organism=Drosophila melanogaster, GI24661184, Length=338, Percent_Identity=43.1952662721894, Blast_Score=301, Evalue=3e-82,
Organism=Drosophila melanogaster, GI161078635, Length=336, Percent_Identity=40.1785714285714, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI161078637, Length=336, Percent_Identity=40.1785714285714, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI161078633, Length=336, Percent_Identity=40.1785714285714, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24650122, Length=336, Percent_Identity=40.1785714285714, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI161078639, Length=331, Percent_Identity=40.4833836858006, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI281362242, Length=339, Percent_Identity=39.8230088495575, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24648872, Length=339, Percent_Identity=39.8230088495575, Blast_Score=218, Evalue=4e-57,
Organism=Drosophila melanogaster, GI20130355, Length=332, Percent_Identity=29.5180722891566, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI45550436, Length=249, Percent_Identity=25.7028112449799, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36104; Mature: 35973

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRKITVIPGDGIGPSIIESAIQILTHAGCDFEYEYADAGLVALEKHGELLPQATLDLI
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEHHCCCCCHHHHHHHH
EKNKVSLKGPLTTPVGGGFTSINVSLRKKFNLYANVRPVISFKGTKSRYENIDIITVREN
HCCCEEECCCCCCCCCCCEEEEEEEEEEHEEEEECCEEEEEECCCCHHHCCEEEEEEECC
TEGMYSGAGQKRSDDNNSAEAMSIVTRDGAERIVKFAFELARQEGRKKVTIIHKANILKS
CCCHHCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHC
TSGLFLEVARDVAARYPDIQSEEMIVDAACMNLVMYPERFDVMVTTNLFGDILSDLCAGL
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHH
VGGLGMAPGANIGDGAAIFEAVHGSAPDIAGKNIANPTSVILAAIQMLEYLGMQDKAERI
HCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
REAVRATIESGDRVTRDLGGTASTSEFTQSIIDRL
HHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKRKITVIPGDGIGPSIIESAIQILTHAGCDFEYEYADAGLVALEKHGELLPQATLDLI
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEHHCCCCCHHHHHHHH
EKNKVSLKGPLTTPVGGGFTSINVSLRKKFNLYANVRPVISFKGTKSRYENIDIITVREN
HCCCEEECCCCCCCCCCCEEEEEEEEEEHEEEEECCEEEEEECCCCHHHCCEEEEEEECC
TEGMYSGAGQKRSDDNNSAEAMSIVTRDGAERIVKFAFELARQEGRKKVTIIHKANILKS
CCCHHCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHC
TSGLFLEVARDVAARYPDIQSEEMIVDAACMNLVMYPERFDVMVTTNLFGDILSDLCAGL
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHH
VGGLGMAPGANIGDGAAIFEAVHGSAPDIAGKNIANPTSVILAAIQMLEYLGMQDKAERI
HCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
REAVRATIESGDRVTRDLGGTASTSEFTQSIIDRL
HHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]