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The map label for this gene is ppnK

Identifier: 145299906

GI number: 145299906

Start: 3232112

End: 3232996

Strand: Direct

Name: ppnK

Synonym: ASA_2998

Alternate gene names: 145299906

Gene position: 3232112-3232996 (Clockwise)

Preceding gene: 145299897

Following gene: 145299907

Centisome position: 68.73

GC content: 60.23

Gene sequence:

>885_bases
ATGGATTCTCCGTTCAAAACCATTGCCCTGATTGGCAAACCCCACCACGAAGGGGCCAACCAGACCCTGACCGGCTTGCA
TCAGTATCTCACTACCCGTGGTTTCAAGGTGCTGGTCGAAAGCCGGGTCGCCCACACCCTCGGCATCATGGATGAAAACG
TGATGGATCTGGTCCAGCTCGGTGAGCAGGCGGACTTGGCCATCGTCGTCGGCGGGGACGGTAACATGCTGGGGGCGGCC
CGAGTGCTGTCACGGTTCGATGTGGCGGTGATCGGTGTCAACCGGGGCAATCTCGGCTTTTTGACTGACCTGTCGCCGCA
GGATTATCTGCTGCCGCTGGAACAGGTGCTTTCCGGCCACTACAAGAGTGAACACCGCTTCTTGCTGGAAGCCGCCGTCT
ATCGTCACGGGGAGCGCAAATCGAGCAACCTGGCGGTGAACGAAGCCGTGCTGCACCCGGGCAAGATAGCCCACATGATC
GAGTTCGAGGTCTATATCGATGGCAGCTTCATGTACAGCCAGCGCTCGGACGGCATCATAGTCGCCACCCCGACCGGCTC
CACCGCCTATTCGCTATCGGCGGGCGGCGCCATCCTCACTCCCAAGCTCAACGCCATCACGCTGGTGCCCATGTTCCCAC
ACACCCTGAGCAGCCGCCCCATAGTGCTGGATGCGGACAGCGAGGTGCGCCTGCTGGTCTCACCGGACAATCAGGATGAT
GCCATGCAGGTGAGCTGCGACGGTCAGGTGACACTGGCGGTACACCCGGGGGACGAGATCCTCATAAAAAAGAGCAAGCA
CAAGCTGCACCTGGTGCATCCGCTCGATTACAGCTACTTCCACGTGCTGCGCAACAAGCTCGGCTGGGGCAGCAAGCTGT
TCTAA

Upstream 100 bases:

>100_bases
CTTTCCTGGTACGATGGCGCGAGCAGAACGCTGGCAGCAGACAAATCCCCCACAAAGCGGGGTATACTGCCCCCAATACT
CATCCCAGACCCGACAGACT

Downstream 100 bases:

>100_bases
GCCTCTTTTAACGTCCCCTGCTCATCCTGTTGCGGGCCGACTCTTTACTGTATGGAAAACCAGTATATACTGGATGCATA
CACAGTAATGTTGGCCCATA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDSPFKTIALIGKPHHEGANQTLTGLHQYLTTRGFKVLVESRVAHTLGIMDENVMDLVQLGEQADLAIVVGGDGNMLGAA
RVLSRFDVAVIGVNRGNLGFLTDLSPQDYLLPLEQVLSGHYKSEHRFLLEAAVYRHGERKSSNLAVNEAVLHPGKIAHMI
EFEVYIDGSFMYSQRSDGIIVATPTGSTAYSLSAGGAILTPKLNAITLVPMFPHTLSSRPIVLDADSEVRLLVSPDNQDD
AMQVSCDGQVTLAVHPGDEILIKKSKHKLHLVHPLDYSYFHVLRNKLGWGSKLF

Sequences:

>Translated_294_residues
MDSPFKTIALIGKPHHEGANQTLTGLHQYLTTRGFKVLVESRVAHTLGIMDENVMDLVQLGEQADLAIVVGGDGNMLGAA
RVLSRFDVAVIGVNRGNLGFLTDLSPQDYLLPLEQVLSGHYKSEHRFLLEAAVYRHGERKSSNLAVNEAVLHPGKIAHMI
EFEVYIDGSFMYSQRSDGIIVATPTGSTAYSLSAGGAILTPKLNAITLVPMFPHTLSSRPIVLDADSEVRLLVSPDNQDD
AMQVSCDGQVTLAVHPGDEILIKKSKHKLHLVHPLDYSYFHVLRNKLGWGSKLF
>Mature_294_residues
MDSPFKTIALIGKPHHEGANQTLTGLHQYLTTRGFKVLVESRVAHTLGIMDENVMDLVQLGEQADLAIVVGGDGNMLGAA
RVLSRFDVAVIGVNRGNLGFLTDLSPQDYLLPLEQVLSGHYKSEHRFLLEAAVYRHGERKSSNLAVNEAVLHPGKIAHMI
EFEVYIDGSFMYSQRSDGIIVATPTGSTAYSLSAGGAILTPKLNAITLVPMFPHTLSSRPIVLDADSEVRLLVSPDNQDD
AMQVSCDGQVTLAVHPGDEILIKKSKHKLHLVHPLDYSYFHVLRNKLGWGSKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=327, Percent_Identity=27.5229357798165, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1788968, Length=294, Percent_Identity=56.1224489795918, Blast_Score=349, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI71999610, Length=150, Percent_Identity=32.6666666666667, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320794, Length=243, Percent_Identity=31.6872427983539, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322509, Length=241, Percent_Identity=32.3651452282158, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325068, Length=210, Percent_Identity=35.7142857142857, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI28573828, Length=253, Percent_Identity=30.4347826086957, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28573826, Length=253, Percent_Identity=30.4347826086957, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28573830, Length=253, Percent_Identity=30.4347826086957, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28573832, Length=253, Percent_Identity=30.4347826086957, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161077047, Length=253, Percent_Identity=30.4347826086957, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281363321, Length=278, Percent_Identity=26.978417266187, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20129957, Length=278, Percent_Identity=26.978417266187, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281363323, Length=278, Percent_Identity=26.978417266187, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653424, Length=278, Percent_Identity=26.978417266187, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653422, Length=278, Percent_Identity=26.978417266187, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_AERS4 (A4SQ27)

Other databases:

- EMBL:   CP000644
- RefSeq:   YP_001142747.1
- ProteinModelPortal:   A4SQ27
- SMR:   A4SQ27
- STRING:   A4SQ27
- GeneID:   4995764
- GenomeReviews:   CP000644_GR
- KEGG:   asa:ASA_2998
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   FESFMID
- PhylomeDB:   A4SQ27
- ProtClustDB:   PRK03378
- BioCyc:   ASAL382245:ASA_2998-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32155; Mature: 32155

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSPFKTIALIGKPHHEGANQTLTGLHQYLTTRGFKVLVESRVAHTLGIMDENVMDLVQL
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHEEHHHHHHHHHHCCCCHHHHHHHC
GEQADLAIVVGGDGNMLGAARVLSRFDVAVIGVNRGNLGFLTDLSPQDYLLPLEQVLSGH
CCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHCCC
YKSEHRFLLEAAVYRHGERKSSNLAVNEAVLHPGKIAHMIEFEVYIDGSFMYSQRSDGII
CCCCHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCEEEEEEEEEEECCEEEECCCCCCEE
VATPTGSTAYSLSAGGAILTPKLNAITLVPMFPHTLSSRPIVLDADSEVRLLVSPDNQDD
EEECCCCCEEEECCCCEEECCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCC
AMQVSCDGQVTLAVHPGDEILIKKSKHKLHLVHPLDYSYFHVLRNKLGWGSKLF
EEEEEECCEEEEEECCCCEEEEEECCCEEEEEECCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDSPFKTIALIGKPHHEGANQTLTGLHQYLTTRGFKVLVESRVAHTLGIMDENVMDLVQL
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHEEHHHHHHHHHHCCCCHHHHHHHC
GEQADLAIVVGGDGNMLGAARVLSRFDVAVIGVNRGNLGFLTDLSPQDYLLPLEQVLSGH
CCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHCCC
YKSEHRFLLEAAVYRHGERKSSNLAVNEAVLHPGKIAHMIEFEVYIDGSFMYSQRSDGII
CCCCHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCCEEEEEEEEEEECCEEEECCCCCCEE
VATPTGSTAYSLSAGGAILTPKLNAITLVPMFPHTLSSRPIVLDADSEVRLLVSPDNQDD
EEECCCCCEEEECCCCEEECCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCC
AMQVSCDGQVTLAVHPGDEILIKKSKHKLHLVHPLDYSYFHVLRNKLGWGSKLF
EEEEEECCEEEEEECCCCEEEEEECCCEEEEEECCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA