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The map label for this gene is smpB [H]

Identifier: 145299836

GI number: 145299836

Start: 3145330

End: 3145818

Strand: Direct

Name: smpB [H]

Synonym: ASA_2922

Alternate gene names: 145299836

Gene position: 3145330-3145818 (Clockwise)

Preceding gene: 145299833

Following gene: 145299837

Centisome position: 66.89

GC content: 56.85

Gene sequence:

>489_bases
ATGAGCAAAAAGAACAGTAAAAACAAAGCCGGGTCCAGCACCATTGCACTCAACAGAACCGCCCGTCACGAGTACTTCAT
CGAAGAACGTGTGGAAGCCGGTCTGTCCCTGCAAGGGTGGGAAGTCAAATCCCTGCGGGCGGGCAAGGCCAATATCAGCG
AAGCCTATGTCATCTTCATGCAAGGCGAAGCCTTCCTGTTCGGCTCCACCTTCTTGCCACTGAATGCCGCCTCCAGCCAC
GTGGTGTGTGACCCGACTCGCACCCGCAAACTCCTGCTGAGCCGCCATGAGCTCGACAAGCTGGAGAGCCTGACTGCCCG
TCAGGGCTACACCATAGTGCCGCTGGCACTTTACTGGAAAGAGTGCTGGGTCAAGGTCGAGATCGGCCTGGTAAAAGGCA
AGAAAGAACACGACAAGCGGGAAGACACCAAGGCCCGCGAATGGGATCGGGAGAAAGCCCGCATCATGAAGAACAAGCAT
CGCGGCTGA

Upstream 100 bases:

>100_bases
GAAACTGCTCACAAAGCCGCCACTTGAGCGGTTGCGCGTTTTACAGCCGCCGCCACCCACGTATAATGCGCCTTCCTAAT
CATTGCCAAAGGCGCCCGTC

Downstream 100 bases:

>100_bases
TTGTGCGCCGCCAGCGCTTGCGCGGCGTCCAATAAACCAGCAATCGACCGGCCAAGGCCGGTCGATTGCTATCAACGCCT
TGCCATGACAGGATTTTTGC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MSKKNSKNKAGSSTIALNRTARHEYFIEERVEAGLSLQGWEVKSLRAGKANISEAYVIFMQGEAFLFGSTFLPLNAASSH
VVCDPTRTRKLLLSRHELDKLESLTARQGYTIVPLALYWKECWVKVEIGLVKGKKEHDKREDTKAREWDREKARIMKNKH
RG

Sequences:

>Translated_162_residues
MSKKNSKNKAGSSTIALNRTARHEYFIEERVEAGLSLQGWEVKSLRAGKANISEAYVIFMQGEAFLFGSTFLPLNAASSH
VVCDPTRTRKLLLSRHELDKLESLTARQGYTIVPLALYWKECWVKVEIGLVKGKKEHDKREDTKAREWDREKARIMKNKH
RG
>Mature_161_residues
SKKNSKNKAGSSTIALNRTARHEYFIEERVEAGLSLQGWEVKSLRAGKANISEAYVIFMQGEAFLFGSTFLPLNAASSHV
VCDPTRTRKLLLSRHELDKLESLTARQGYTIVPLALYWKECWVKVEIGLVKGKKEHDKREDTKAREWDREKARIMKNKHR
G

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=161, Percent_Identity=67.0807453416149, Blast_Score=227, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18562; Mature: 18430

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKNSKNKAGSSTIALNRTARHEYFIEERVEAGLSLQGWEVKSLRAGKANISEAYVIFM
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEEEEEE
QGEAFLFGSTFLPLNAASSHVVCDPTRTRKLLLSRHELDKLESLTARQGYTIVPLALYWK
CCCEEEECCEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
ECWVKVEIGLVKGKKEHDKREDTKAREWDREKARIMKNKHRG
HCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKKNSKNKAGSSTIALNRTARHEYFIEERVEAGLSLQGWEVKSLRAGKANISEAYVIFM
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEEEEEE
QGEAFLFGSTFLPLNAASSHVVCDPTRTRKLLLSRHELDKLESLTARQGYTIVPLALYWK
CCCEEEECCEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
ECWVKVEIGLVKGKKEHDKREDTKAREWDREKARIMKNKHRG
HCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA