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The map label for this gene is hlyB [H]

Identifier: 145299813

GI number: 145299813

Start: 3120543

End: 3122165

Strand: Direct

Name: hlyB [H]

Synonym: ASA_2895

Alternate gene names: 145299813

Gene position: 3120543-3122165 (Clockwise)

Preceding gene: 145299811

Following gene: 145299814

Centisome position: 66.36

GC content: 62.85

Gene sequence:

>1623_bases
ATGTCGTTGAGTAACCTGTCCATCCGTCTTCAAGTGCTTGTTCCACTCCTGCTCGCCAGCCTCTTGATGGTCCTGATGGC
CGGGATCAGCAAGCAGGAGATAGACAGCGCCATTGCGGACATGAACCAGACCACCACCAGTGTGACGCGCCACAAGGACG
ACATCGCTGCCCTGATCCACGCCACCTACCGGATGCGCACCAACGCCATCTATGGCCTGTACGATCCGGCGCGCTTCGCC
CAGTTGCCAAACGATCTCAATGCGGCGGAACAGGAAATAGGGACCATCTTGTCCCGACTGGTGATCTCAGGTGCCGAGCA
GGACAACAGCCAGGTGGCCGCCAGCCTGCAGGCTTATCTCGGCCACACCCGCAACAACATGCTGCCGCTGCTGGCCCAGA
AACATCAGGGAGGTGGCGATGCCGCCGCCTACGAGCAGTCACGGCTGCGTTTTCGTGAGCTGGGGGAGCAGTTGATCAAG
CAGATCGATACCATGTCAGCGCGCATCAACCAGCTGATCCAGACCGAAATTGCCGAGGAAGAGGCGCACTACAACGCCAC
CCTGTTGCGAACCCTGCTGATGATGAGTGCCACCCTGATAGCGGCGCTGCTCGGTGGCTGGTGGTTGTCCGGCCAGATAG
TCCGCCCCATAGGCCAGTTGCAGGAGGTGATGCGCGCCGTCGCCCAGGGGCGCTTCAACGTGCGGGCCAACGCCGATGGC
AGCAACGAACTGAGCCAGCTGGCCAAAGATATCAACCAGACACTGACGCAGCTTGGCAGCACAATCCACACCCTGACCGG
TATCAGCGGCAACGTGGCCTCTGCCGCCACCGAGCTCGCCGCCGTCATGACCCAGTCCGAGACCAATGCCCAGCAGCAGC
GCAGCGAGACAGAACAGGTGGCCTCCGCCGTGACCGAGCTGGCCAGCACGGCCGACAACGTCAACCACAACGCCGCCCTG
GCAGATGGGCTGGCGCGGGATGCCAATAGCCGGGTCGAACAGGGGCTGGCTCTGTTCGCGGAGAGTATCGCTGCCAACAG
CCGCATGGCGGGCAGCCTGGAAAGCGCCGCCGCCGTGGTGGCGCGCCTCAAGTCCCAGTCCGAGCAGATCGGCAAGGTGA
TCGAAGTGATCCAGAGCATCTCGGAGCAGACCAACCTGCTGGCCCTGAATGCCGCCATCGAGGCTGCTCGCGCCGGCGAA
ACCGGTCGCGGCTTCGCGGTCGTGGCCGACGAGGTACGCCTGCTGGCGGCCCGCACGCAGGACTCCACCAAGGAGATCCA
GACCATCATAGAGCAGTTGCAGAGCCAGTCCCAATCCGCCAACAACGGCATGCAGGAGACCCTCACCATCCTTGAGCACA
ACCAGCAGCTCTCCGGCGAGGTGCAGGCGCTGCTCGGTGGCATCACGGAGGCGGTCAACCAGATCAATGGCGCCAATGCC
CAGGTGGCCACCGCCGCCGAAGAGCAGTCGTGCGTCACCGCCGACATCAACCGCAACATCACCAATATTTACGAAATAGT
GAACCAGAGTGTGGCCGGCATCAGCCAGAGTGCCGCCGCCAGTCACGAACTGTCACAGCTCGCCGAGCTGCAGCGCAAAC
AGCTGGCCCAGTTCCAGTTGTGA

Upstream 100 bases:

>100_bases
CTTTTCGTTCAGCATGATTTCAGCAATAAAAACCCTGTGACAATAGTTGTCGCGCCTGTGTAATATGCCGCCTCACTCCT
CTTTTGGGTTAGGACCTGCC

Downstream 100 bases:

>100_bases
CGTCCGCTTGCGCGATCGACACAAAGGCCCCGCTTGCGGGGCCTTTGTGTTAATGAATGGACACATTTGTTATTGTTTTA
AACGGCTTTTTTATAATGTG

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSLSNLSIRLQVLVPLLLASLLMVLMAGISKQEIDSAIADMNQTTTSVTRHKDDIAALIHATYRMRTNAIYGLYDPARFA
QLPNDLNAAEQEIGTILSRLVISGAEQDNSQVAASLQAYLGHTRNNMLPLLAQKHQGGGDAAAYEQSRLRFRELGEQLIK
QIDTMSARINQLIQTEIAEEEAHYNATLLRTLLMMSATLIAALLGGWWLSGQIVRPIGQLQEVMRAVAQGRFNVRANADG
SNELSQLAKDINQTLTQLGSTIHTLTGISGNVASAATELAAVMTQSETNAQQQRSETEQVASAVTELASTADNVNHNAAL
ADGLARDANSRVEQGLALFAESIAANSRMAGSLESAAAVVARLKSQSEQIGKVIEVIQSISEQTNLLALNAAIEAARAGE
TGRGFAVVADEVRLLAARTQDSTKEIQTIIEQLQSQSQSANNGMQETLTILEHNQQLSGEVQALLGGITEAVNQINGANA
QVATAAEEQSCVTADINRNITNIYEIVNQSVAGISQSAAASHELSQLAELQRKQLAQFQL

Sequences:

>Translated_540_residues
MSLSNLSIRLQVLVPLLLASLLMVLMAGISKQEIDSAIADMNQTTTSVTRHKDDIAALIHATYRMRTNAIYGLYDPARFA
QLPNDLNAAEQEIGTILSRLVISGAEQDNSQVAASLQAYLGHTRNNMLPLLAQKHQGGGDAAAYEQSRLRFRELGEQLIK
QIDTMSARINQLIQTEIAEEEAHYNATLLRTLLMMSATLIAALLGGWWLSGQIVRPIGQLQEVMRAVAQGRFNVRANADG
SNELSQLAKDINQTLTQLGSTIHTLTGISGNVASAATELAAVMTQSETNAQQQRSETEQVASAVTELASTADNVNHNAAL
ADGLARDANSRVEQGLALFAESIAANSRMAGSLESAAAVVARLKSQSEQIGKVIEVIQSISEQTNLLALNAAIEAARAGE
TGRGFAVVADEVRLLAARTQDSTKEIQTIIEQLQSQSQSANNGMQETLTILEHNQQLSGEVQALLGGITEAVNQINGANA
QVATAAEEQSCVTADINRNITNIYEIVNQSVAGISQSAAASHELSQLAELQRKQLAQFQL
>Mature_539_residues
SLSNLSIRLQVLVPLLLASLLMVLMAGISKQEIDSAIADMNQTTTSVTRHKDDIAALIHATYRMRTNAIYGLYDPARFAQ
LPNDLNAAEQEIGTILSRLVISGAEQDNSQVAASLQAYLGHTRNNMLPLLAQKHQGGGDAAAYEQSRLRFRELGEQLIKQ
IDTMSARINQLIQTEIAEEEAHYNATLLRTLLMMSATLIAALLGGWWLSGQIVRPIGQLQEVMRAVAQGRFNVRANADGS
NELSQLAKDINQTLTQLGSTIHTLTGISGNVASAATELAAVMTQSETNAQQQRSETEQVASAVTELASTADNVNHNAALA
DGLARDANSRVEQGLALFAESIAANSRMAGSLESAAAVVARLKSQSEQIGKVIEVIQSISEQTNLLALNAAIEAARAGET
GRGFAVVADEVRLLAARTQDSTKEIQTIIEQLQSQSQSANNGMQETLTILEHNQQLSGEVQALLGGITEAVNQINGANAQ
VATAAEEQSCVTADINRNITNIYEIVNQSVAGISQSAAASHELSQLAELQRKQLAQFQL

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=424, Percent_Identity=26.8867924528302, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1788194, Length=374, Percent_Identity=26.7379679144385, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI2367378, Length=315, Percent_Identity=30.1587301587302, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1788195, Length=285, Percent_Identity=29.8245614035088, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789453, Length=343, Percent_Identity=28.2798833819242, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 57807; Mature: 57676

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSNLSIRLQVLVPLLLASLLMVLMAGISKQEIDSAIADMNQTTTSVTRHKDDIAALIH
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATYRMRTNAIYGLYDPARFAQLPNDLNAAEQEIGTILSRLVISGAEQDNSQVAASLQAYL
HHHHHHHCCEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
GHTRNNMLPLLAQKHQGGGDAAAYEQSRLRFRELGEQLIKQIDTMSARINQLIQTEIAEE
HHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAHYNATLLRTLLMMSATLIAALLGGWWLSGQIVRPIGQLQEVMRAVAQGRFNVRANADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
SNELSQLAKDINQTLTQLGSTIHTLTGISGNVASAATELAAVMTQSETNAQQQRSETEQV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ASAVTELASTADNVNHNAALADGLARDANSRVEQGLALFAESIAANSRMAGSLESAAAVV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHH
ARLKSQSEQIGKVIEVIQSISEQTNLLALNAAIEAARAGETGRGFAVVADEVRLLAARTQ
HHHHHHHHHHHHHHHHHHHHHHHHCEEHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHCC
DSTKEIQTIIEQLQSQSQSANNGMQETLTILEHNQQLSGEVQALLGGITEAVNQINGANA
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
QVATAAEEQSCVTADINRNITNIYEIVNQSVAGISQSAAASHELSQLAELQRKQLAQFQL
CEEEHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLSNLSIRLQVLVPLLLASLLMVLMAGISKQEIDSAIADMNQTTTSVTRHKDDIAALIH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATYRMRTNAIYGLYDPARFAQLPNDLNAAEQEIGTILSRLVISGAEQDNSQVAASLQAYL
HHHHHHHCCEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
GHTRNNMLPLLAQKHQGGGDAAAYEQSRLRFRELGEQLIKQIDTMSARINQLIQTEIAEE
HHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAHYNATLLRTLLMMSATLIAALLGGWWLSGQIVRPIGQLQEVMRAVAQGRFNVRANADG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
SNELSQLAKDINQTLTQLGSTIHTLTGISGNVASAATELAAVMTQSETNAQQQRSETEQV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ASAVTELASTADNVNHNAALADGLARDANSRVEQGLALFAESIAANSRMAGSLESAAAVV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHH
ARLKSQSEQIGKVIEVIQSISEQTNLLALNAAIEAARAGETGRGFAVVADEVRLLAARTQ
HHHHHHHHHHHHHHHHHHHHHHHHCEEHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHCC
DSTKEIQTIIEQLQSQSQSANNGMQETLTILEHNQQLSGEVQALLGGITEAVNQINGANA
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCC
QVATAAEEQSCVTADINRNITNIYEIVNQSVAGISQSAAASHELSQLAELQRKQLAQFQL
CEEEHHHHCCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]