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The map label for this gene is ilvA [H]

Identifier: 145296088

GI number: 145296088

Start: 2208859

End: 2210130

Strand: Reverse

Name: ilvA [H]

Synonym: cgR_2010

Alternate gene names: 145296088

Gene position: 2210130-2208859 (Counterclockwise)

Preceding gene: 145296090

Following gene: 145296086

Centisome position: 66.69

GC content: 55.03

Gene sequence:

>1272_bases
ATGGCTAGCGGAGCGGAGCTGATTCGTGCCGCCGACATTCAAACGGCGCAGGCACGAATTTCCTCCGTCATTGCACCAAC
TCCATTGCAGTATTGCCCTCGTCTTTCTGAGGAAACCGGAGCGGAAATCTACCTTAAGCGTGAGGATCTGCAGGATGTTC
GTTCCTACAAGATCCGCGGTGCGCTGAACTCTGGAGCGCAGCTCACTCAGGAGCAGCGTGATGCAGGTATCGTTGCCGCA
TCTGCAGGTAACCATGCCCAGGGCGTGGCCTATGTGTGCAAGTCCTTGGGCGTTCAGGGACGCATCTATGTTCCTGTGCA
GACTCCAAAGCAAAAGCGTGACCGCATCATGGTTCATGGTGGAGAGTTTGTCTCCTTGGTGGTCACCGGCAACAACTTTG
ATGAAGCATCGGCTGCAGCGCATGAAGATGCAGAGCGCACCGGCGCAACGCTGATCGAGCCATTTGATGCTCGCAACACC
GTCATTGGTCAGGGCACCGTGGCTGCTGAGATCTTGTCGCAGCTGACTTCCATGGGCAAGAGCGCTGATCATGTGATGGT
GCCAGTAGGTGGCGGCGGTCTACTGGCGGGTGTGGTCAGCTACATGGCTGATATGGCACCACGCACTGCGATCGTTGGAA
TTGAGCCTGCGGGAGCAGCATCCATGCAGGCTGCATTGCACAATGGTGGACCAATCACTTTGGAGACTGTTGATCCCTTT
GTGGACGGCGCAGCAGTCAAACGTGTCGGAGATCTCAACTACACCATCGTGGAGAAGAACTTGGGTCGCGTACACATGAT
GAGTGCCACCGAGGGCGCTGTGTGTACTGAGATGCTTGATCTCTACCAAAACGAAGGCATCATCGCGGAGCCTGCCGGTG
CACTGTCTATCGCAGGGTTGAAGGAAATGTCCTTCGCGCCTGGTTCTGTCGTGGTGTGCATCATCTCTGGTGGCAACAAC
GATGTGCTGCGTTATGCGGAAATCGCTGAGCGCTCCTTGGTACACCGCGGTTTGAAGCATTACTTCTTGGTGAACTTCCC
GCAAAAGCCTGGTCAGTTGCGTCACTTCCTGGAAGATATCCTGGGACCGGATGATGACATCACGCTGTTTGAGTACCTCA
AGCGCAACAACCGTGAGACCGGTACTGCGTTGGTGGGTATTCACTTGAGTGAAGCATCAGGATTGGATTCTTTGCTGGAA
CGTATGGAGGAATCGGCAATTGATTCCCGTCGCCTCGAGCCGGGAACCCCTGAGTACGAATACTTGACCTAA

Upstream 100 bases:

>100_bases
TTGGAAATAAAACCTATGCCAAAGTAGGTGCAATTCTAGGAGAGGATTACACTAGTCAACCATGAGTGAAACATACGTGT
CTGAGAAAAGTCCAGGAGTG

Downstream 100 bases:

>100_bases
ACATAGCTGAAGGCCACCTCAATCGAGGTGGCCTTTTTCTAGTTTCGGGTCAGGATCGCAAAGCCCCACGGCTGAAGGGT
TGTGGAGGTGTCGGTGACAG

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MASGAELIRAADIQTAQARISSVIAPTPLQYCPRLSEETGAEIYLKREDLQDVRSYKIRGALNSGAQLTQEQRDAGIVAA
SAGNHAQGVAYVCKSLGVQGRIYVPVQTPKQKRDRIMVHGGEFVSLVVTGNNFDEASAAAHEDAERTGATLIEPFDARNT
VIGQGTVAAEILSQLTSMGKSADHVMVPVGGGGLLAGVVSYMADMAPRTAIVGIEPAGAASMQAALHNGGPITLETVDPF
VDGAAVKRVGDLNYTIVEKNLGRVHMMSATEGAVCTEMLDLYQNEGIIAEPAGALSIAGLKEMSFAPGSVVVCIISGGNN
DVLRYAEIAERSLVHRGLKHYFLVNFPQKPGQLRHFLEDILGPDDDITLFEYLKRNNRETGTALVGIHLSEASGLDSLLE
RMEESAIDSRRLEPGTPEYEYLT

Sequences:

>Translated_423_residues
MASGAELIRAADIQTAQARISSVIAPTPLQYCPRLSEETGAEIYLKREDLQDVRSYKIRGALNSGAQLTQEQRDAGIVAA
SAGNHAQGVAYVCKSLGVQGRIYVPVQTPKQKRDRIMVHGGEFVSLVVTGNNFDEASAAAHEDAERTGATLIEPFDARNT
VIGQGTVAAEILSQLTSMGKSADHVMVPVGGGGLLAGVVSYMADMAPRTAIVGIEPAGAASMQAALHNGGPITLETVDPF
VDGAAVKRVGDLNYTIVEKNLGRVHMMSATEGAVCTEMLDLYQNEGIIAEPAGALSIAGLKEMSFAPGSVVVCIISGGNN
DVLRYAEIAERSLVHRGLKHYFLVNFPQKPGQLRHFLEDILGPDDDITLFEYLKRNNRETGTALVGIHLSEASGLDSLLE
RMEESAIDSRRLEPGTPEYEYLT
>Mature_422_residues
ASGAELIRAADIQTAQARISSVIAPTPLQYCPRLSEETGAEIYLKREDLQDVRSYKIRGALNSGAQLTQEQRDAGIVAAS
AGNHAQGVAYVCKSLGVQGRIYVPVQTPKQKRDRIMVHGGEFVSLVVTGNNFDEASAAAHEDAERTGATLIEPFDARNTV
IGQGTVAAEILSQLTSMGKSADHVMVPVGGGGLLAGVVSYMADMAPRTAIVGIEPAGAASMQAALHNGGPITLETVDPFV
DGAAVKRVGDLNYTIVEKNLGRVHMMSATEGAVCTEMLDLYQNEGIIAEPAGALSIAGLKEMSFAPGSVVVCIISGGNND
VLRYAEIAERSLVHRGLKHYFLVNFPQKPGQLRHFLEDILGPDDDITLFEYLKRNNRETGTALVGIHLSEASGLDSLLER
MEESAIDSRRLEPGTPEYEYLT

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=335, Percent_Identity=27.7611940298507, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1790207, Length=392, Percent_Identity=36.2244897959184, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789505, Length=321, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1789235, Length=207, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71991565, Length=346, Percent_Identity=29.7687861271676, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17537387, Length=381, Percent_Identity=30.1837270341207, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17508781, Length=283, Percent_Identity=29.3286219081272, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320930, Length=376, Percent_Identity=35.3723404255319, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6322631, Length=320, Percent_Identity=31.875, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6319788, Length=236, Percent_Identity=27.5423728813559, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21355833, Length=345, Percent_Identity=31.0144927536232, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645328, Length=228, Percent_Identity=30.7017543859649, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 45162; Mature: 45031

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGAELIRAADIQTAQARISSVIAPTPLQYCPRLSEETGAEIYLKREDLQDVRSYKIRG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEEHHHHHHHHHEEECC
ALNSGAQLTQEQRDAGIVAASAGNHAQGVAYVCKSLGVQGRIYVPVQTPKQKRDRIMVHG
CCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHCCCEEEEEC
GEFVSLVVTGNNFDEASAAAHEDAERTGATLIEPFDARNTVIGQGTVAAEILSQLTSMGK
CCEEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCCCCEEEECCHHHHHHHHHHHHCCC
SADHVMVPVGGGGLLAGVVSYMADMAPRTAIVGIEPAGAASMQAALHNGGPITLETVDPF
CCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCH
VDGAAVKRVGDLNYTIVEKNLGRVHMMSATEGAVCTEMLDLYQNEGIIAEPAGALSIAGL
HCCHHHHHHCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEECCH
KEMSFAPGSVVVCIISGGNNDVLRYAEIAERSLVHRGLKHYFLVNFPQKPGQLRHFLEDI
HHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
LGPDDDITLFEYLKRNNRETGTALVGIHLSEASGLDSLLERMEESAIDSRRLEPGTPEYE
CCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
YLT
CCC
>Mature Secondary Structure 
ASGAELIRAADIQTAQARISSVIAPTPLQYCPRLSEETGAEIYLKREDLQDVRSYKIRG
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEEHHHHHHHHHEEECC
ALNSGAQLTQEQRDAGIVAASAGNHAQGVAYVCKSLGVQGRIYVPVQTPKQKRDRIMVHG
CCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHCCCEEEEEC
GEFVSLVVTGNNFDEASAAAHEDAERTGATLIEPFDARNTVIGQGTVAAEILSQLTSMGK
CCEEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCCCCEEEECCHHHHHHHHHHHHCCC
SADHVMVPVGGGGLLAGVVSYMADMAPRTAIVGIEPAGAASMQAALHNGGPITLETVDPF
CCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCH
VDGAAVKRVGDLNYTIVEKNLGRVHMMSATEGAVCTEMLDLYQNEGIIAEPAGALSIAGL
HCCHHHHHHCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEECCH
KEMSFAPGSVVVCIISGGNNDVLRYAEIAERSLVHRGLKHYFLVNFPQKPGQLRHFLEDI
HHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHH
LGPDDDITLFEYLKRNNRETGTALVGIHLSEASGLDSLLERMEESAIDSRRLEPGTPEYE
CCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
YLT
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1459955; 12948626 [H]