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The map label for this gene is hisF [H]

Identifier: 145296056

GI number: 145296056

Start: 2175943

End: 2176719

Strand: Reverse

Name: hisF [H]

Synonym: cgR_1978

Alternate gene names: 145296056

Gene position: 2176719-2175943 (Counterclockwise)

Preceding gene: 145296057

Following gene: 145296055

Centisome position: 65.68

GC content: 56.76

Gene sequence:

>777_bases
ATGGGCGTGGCAATTCGAGTTATTCCTTGCCTGGACGTGGACAACGGCCGGGTTGTTAAAGGCGTGAACTTTGAAAACCT
CCGCGATGCTGGCGATCCTGTGGAGTTGGCAAAGCGCTATGACGAGGAAGGGGCAGATGAGCTGACCTTCCTGGATGTCA
CCGCCTCGAAGCATGGTCGCGGCACCATGCTGGATGTTGTTCGACGCACCGCTGATCAGGTGTTCATCCCTCTGACTGTC
GGTGGCGGCGTGCGTAGCGAAGAAGATGTTGATCAATTGCTGCGCGCTGGCGCCGACAAGGTTTCGGTGAACACATCTGC
GATTGCCCGTCCAGAATTGCTGTCAGAGCTGTCCAAGCGTTTTGGTGCTCAGTGCATCGTGTTGTCTGTGGATGCCAGGC
GCGTTCCTGAAGGTGGAACTCCTCAGCCATCTGGTTTTGAAGTCACCACCCACGGCGGTTCCAAGTCCGCAGAACTTGAT
GCAATCGAGTGGGCAAAGCGCGGCGAAGAGCTGGGCGTTGGCGAAATTCTGCTCAACTCCATGGACGGCGACGGCACCAA
AAACGGCTTTGACCTAGAGCTGCTGGAAAAAGTTCGCGCAGCCGTATCCATTCCTGTAATCGCCTCCGGCGGCGCTGGCA
AGGCGGAGCATTTCCCACCAGCTGTTGCAGCTGGCGCCAACGCAGTGCTTGCCGCGACCATTTTCCACTTCCGCGAAGTA
ACCATCGCCGAAGTAAAGGGAGCCATTAAAGATGCAGGATTTGAGGTGCGGAAATGA

Upstream 100 bases:

>100_bases
AAAAGGGCTCACGATGTGCTGTTAAGTAAGATTGAAAAAGTTCGGTTGATGCATGCAGATGCAGGTAATGACCAGTCGTT
AAATGAGGAGTACAAGTAAA

Downstream 100 bases:

>100_bases
GTGACAATCCACAAGAGTATGAGCTGGATTGGGACGTCGAAAAGCGATTAAAGCTTAACGACGCCGGCCTGGTGCCGGCA
ATCGTCCAGGCCGACGGGAC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MGVAIRVIPCLDVDNGRVVKGVNFENLRDAGDPVELAKRYDEEGADELTFLDVTASKHGRGTMLDVVRRTADQVFIPLTV
GGGVRSEEDVDQLLRAGADKVSVNTSAIARPELLSELSKRFGAQCIVLSVDARRVPEGGTPQPSGFEVTTHGGSKSAELD
AIEWAKRGEELGVGEILLNSMDGDGTKNGFDLELLEKVRAAVSIPVIASGGAGKAEHFPPAVAAGANAVLAATIFHFREV
TIAEVKGAIKDAGFEVRK

Sequences:

>Translated_258_residues
MGVAIRVIPCLDVDNGRVVKGVNFENLRDAGDPVELAKRYDEEGADELTFLDVTASKHGRGTMLDVVRRTADQVFIPLTV
GGGVRSEEDVDQLLRAGADKVSVNTSAIARPELLSELSKRFGAQCIVLSVDARRVPEGGTPQPSGFEVTTHGGSKSAELD
AIEWAKRGEELGVGEILLNSMDGDGTKNGFDLELLEKVRAAVSIPVIASGGAGKAEHFPPAVAAGANAVLAATIFHFREV
TIAEVKGAIKDAGFEVRK
>Mature_257_residues
GVAIRVIPCLDVDNGRVVKGVNFENLRDAGDPVELAKRYDEEGADELTFLDVTASKHGRGTMLDVVRRTADQVFIPLTVG
GGVRSEEDVDQLLRAGADKVSVNTSAIARPELLSELSKRFGAQCIVLSVDARRVPEGGTPQPSGFEVTTHGGSKSAELDA
IEWAKRGEELGVGEILLNSMDGDGTKNGFDLELLEKVRAAVSIPVIASGGAGKAEHFPPAVAAGANAVLAATIFHFREVT
IAEVKGAIKDAGFEVRK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=45, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI87082028, Length=250, Percent_Identity=28.4, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=39.0476190476191, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27245; Mature: 27114

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVAIRVIPCLDVDNGRVVKGVNFENLRDAGDPVELAKRYDEEGADELTFLDVTASKHGR
CCEEEEEEEEEECCCCCEEECCCHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCC
GTMLDVVRRTADQVFIPLTVGGGVRSEEDVDQLLRAGADKVSVNTSAIARPELLSELSKR
CHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHCCHHHHHHHHHH
FGAQCIVLSVDARRVPEGGTPQPSGFEVTTHGGSKSAELDAIEWAKRGEELGVGEILLNS
HCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCC
MDGDGTKNGFDLELLEKVRAAVSIPVIASGGAGKAEHFPPAVAAGANAVLAATIFHFREV
CCCCCCCCCCCHHHHHHHHHHEECCEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
TIAEVKGAIKDAGFEVRK
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GVAIRVIPCLDVDNGRVVKGVNFENLRDAGDPVELAKRYDEEGADELTFLDVTASKHGR
CEEEEEEEEEECCCCCEEECCCHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCC
GTMLDVVRRTADQVFIPLTVGGGVRSEEDVDQLLRAGADKVSVNTSAIARPELLSELSKR
CHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHCCHHHHHHHHHH
FGAQCIVLSVDARRVPEGGTPQPSGFEVTTHGGSKSAELDAIEWAKRGEELGVGEILLNS
HCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCC
MDGDGTKNGFDLELLEKVRAAVSIPVIASGGAGKAEHFPPAVAAGANAVLAATIFHFREV
CCCCCCCCCCCHHHHHHHHHHEECCEEECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHH
TIAEVKGAIKDAGFEVRK
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]