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The map label for this gene is tsf [H]

Identifier: 145295928

GI number: 145295928

Start: 2058011

End: 2058838

Strand: Reverse

Name: tsf [H]

Synonym: cgR_1853

Alternate gene names: 145295928

Gene position: 2058838-2058011 (Counterclockwise)

Preceding gene: 145295929

Following gene: 145295927

Centisome position: 62.12

GC content: 57.0

Gene sequence:

>828_bases
ATGGCGAACTACACCGCTGCGGATGTTAAGAAGCTCCGCGAACTCACCGGTTCCGGCATGCTCGATTGCAAGAAGGCTCT
GGAGGAGTCCGCTGGCGACTTCGACAAGGCTGTTGAGATCCTGCGCGTCAAGGGCGCAAAGGACGTCGGAAAGCGTGCAG
AGCGTAACGCTACCGAGGGTCTCGTTGCAGTTTCTGGCAACACCATGATCGAGGTCAACTCTGAGACCGACTTCGTCGCA
AAGAACTCTGAGTTCAAGGAATTCGCTGCAAACGTTGCAGACGCAGCAGCAGCTGCAAAGGCTAACTCCCAGGAAGAGCT
CGCAGCAGTTGACGTGGACGGAAAGACCGCAGACGCAGCTCTGCAGGAGTTCTCCGCAAAGATCGGCGAGAAGCTTGAGC
TTCGCCGCGCAGTTACCCTCGAGGGCGACAAGACCGCTGTTTACCTCCACCAGCGTTCCGCTGACCTGCCACCAGCAGTT
GGCGTTTTGGTTGCTTTCACCGGTGAAGGTGAAGCAGCTGAGGCAGCTGCACGTCAGGCTGCAATGCAGATTGCTGCTCT
GAAGGCTTCTTACCTCACCCGTGAGGACGTTCCTGCAGAGATCATCGAGAAGGAGCGCTCCATCGCTGAGCAGATCACTC
GCGAAGAGGGCAAGCCAGAGCAGGCTATCCCTAAGATCGTTGAGGGTCGTCTGAACGGCTTCTACAAGGAGAACGTTCTT
CTTGAGCAGTCTTCCGTAGCTGACAGCAAGAAGACCGTTAAGGCTCTTCTCGACGAGGCTGGCGTTACCGTCACCTCTTT
CGCTCGCTTCGAGGTCGGCCAGGCTTAA

Upstream 100 bases:

>100_bases
ACCTGTACGATCACTTTTTAGACGGGCGGGTAGGGCTACTGTGCCCTAACCTAAGCTTGTAAAGCATTAATTATCCATAC
ATAAGGAGGATCGCCCCGTA

Downstream 100 bases:

>100_bases
GGCCACTTGAAGGTTGTGGGTGGGTGTCTGTTGATCCCCACCTCGACTTTTTAAGTTTTGAAAAAGACTTAAACACTTTA
CAAGGTTGCCCCGCGCATCG

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MANYTAADVKKLRELTGSGMLDCKKALEESAGDFDKAVEILRVKGAKDVGKRAERNATEGLVAVSGNTMIEVNSETDFVA
KNSEFKEFAANVADAAAAAKANSQEELAAVDVDGKTADAALQEFSAKIGEKLELRRAVTLEGDKTAVYLHQRSADLPPAV
GVLVAFTGEGEAAEAAARQAAMQIAALKASYLTREDVPAEIIEKERSIAEQITREEGKPEQAIPKIVEGRLNGFYKENVL
LEQSSVADSKKTVKALLDEAGVTVTSFARFEVGQA

Sequences:

>Translated_275_residues
MANYTAADVKKLRELTGSGMLDCKKALEESAGDFDKAVEILRVKGAKDVGKRAERNATEGLVAVSGNTMIEVNSETDFVA
KNSEFKEFAANVADAAAAAKANSQEELAAVDVDGKTADAALQEFSAKIGEKLELRRAVTLEGDKTAVYLHQRSADLPPAV
GVLVAFTGEGEAAEAAARQAAMQIAALKASYLTREDVPAEIIEKERSIAEQITREEGKPEQAIPKIVEGRLNGFYKENVL
LEQSSVADSKKTVKALLDEAGVTVTSFARFEVGQA
>Mature_274_residues
ANYTAADVKKLRELTGSGMLDCKKALEESAGDFDKAVEILRVKGAKDVGKRAERNATEGLVAVSGNTMIEVNSETDFVAK
NSEFKEFAANVADAAAAAKANSQEELAAVDVDGKTADAALQEFSAKIGEKLELRRAVTLEGDKTAVYLHQRSADLPPAVG
VLVAFTGEGEAAEAAARQAAMQIAALKASYLTREDVPAEIIEKERSIAEQITREEGKPEQAIPKIVEGRLNGFYKENVLL
EQSSVADSKKTVKALLDEAGVTVTSFARFEVGQA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Escherichia coli, GI1786366, Length=278, Percent_Identity=42.0863309352518, Blast_Score=177, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17561440, Length=294, Percent_Identity=25.8503401360544, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19921466, Length=291, Percent_Identity=28.8659793814433, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 29294; Mature: 29163

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANYTAADVKKLRELTGSGMLDCKKALEESAGDFDKAVEILRVKGAKDVGKRAERNATEG
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCHHCC
LVAVSGNTMIEVNSETDFVAKNSEFKEFAANVADAAAAAKANSQEELAAVDVDGKTADAA
EEEECCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCHHHHH
LQEFSAKIGEKLELRRAVTLEGDKTAVYLHQRSADLPPAVGVLVAFTGEGEAAEAAARQA
HHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHH
AMQIAALKASYLTREDVPAEIIEKERSIAEQITREEGKPEQAIPKIVEGRLNGFYKENVL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHCEE
LEQSSVADSKKTVKALLDEAGVTVTSFARFEVGQA
EEHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCC
>Mature Secondary Structure 
ANYTAADVKKLRELTGSGMLDCKKALEESAGDFDKAVEILRVKGAKDVGKRAERNATEG
CCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCHHCC
LVAVSGNTMIEVNSETDFVAKNSEFKEFAANVADAAAAAKANSQEELAAVDVDGKTADAA
EEEECCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCHHHHH
LQEFSAKIGEKLELRRAVTLEGDKTAVYLHQRSADLPPAVGVLVAFTGEGEAAEAAARQA
HHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHH
AMQIAALKASYLTREDVPAEIIEKERSIAEQITREEGKPEQAIPKIVEGRLNGFYKENVL
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHCEE
LEQSSVADSKKTVKALLDEAGVTVTSFARFEVGQA
EEHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]