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The map label for this gene is rlmN [H]

Identifier: 145295923

GI number: 145295923

Start: 2053462

End: 2054562

Strand: Reverse

Name: rlmN [H]

Synonym: cgR_1848

Alternate gene names: 145295923

Gene position: 2054562-2053462 (Counterclockwise)

Preceding gene: 145295925

Following gene: 145295922

Centisome position: 61.99

GC content: 54.41

Gene sequence:

>1101_bases
ATGGCTACACCTGTACCACTGGTTTTTAACGCACCTAAGCGTGGCATGCCCCCAACCCACTTTGCGGATCTTAATGATGA
GGCTCGCATTGAGGCTCTTAAAGAGCTCGGCTTGCCCAAATTCCGACTCAATCAGATCGCTCGACACTACTATGGTCGCC
TCGAGGCTGACCCACTCACTATGACGGACCTGCCTGAAGGTGCTCGCCAAGAAGTAAAAGATGCTCTTTTCCCAACGTTG
ATGTCTCCGCTGCGTGTCATTGAAACTGATGACGATACGACTCAGAAGACGTTATGGAAGCTCCATGACGGCACTTTGCT
TGAGTCTGTTCTCATGCGCTATTCAGATCGCTCCACGCTGTGTATTTCTTCGCAGGCTGGTTGCGGTATGGCGTGCCCAT
TCTGCGCAACTGGTCAGGGTGGTTTGGACCGTAACCTTTCGATCGGTGAGATCGTGGATCAGGTTCGTAATGCTGCTGCA
ACGATGCAGGCAGAGGGCGGTCGCCTGTCCAACATTGTGTTCATGGGAATGGGCGAGCCGCTTGCTAACTACAAGCGTGT
GGTGTCGGCTGTTCGTCAGATCACGCAGCCAAGCCCTGCGGGCTTCGGCATTTCCCAGCGCAGTGTGACTGTCTCCACCG
TGGGCCTCGCTCCAGCTATCAGGAAGCTTGCCGACGAAGAGATGTCCGTAACTTTGGCAGTTTCCTTGCACACTCCAGAC
GATGAGTTGCGTGACACTCTCGTGCCAGTCAACAACCGTTGGCCTGTCGCTGAGGTACTGGACGCTGCTCGTTACTACGC
AGATAAGTCTGGCCGTCGCGTCTCCATCGAGTATGCGCTCATTCGCGATGTGAATGACCAGGATTGGCGCGCAGATATGC
TGGGCGAGAAGCTGCATAAGGCTTTGGGCTCCCGTGTGCACGTCAACTTGATTCCATTGAACCCAACTCCTGGTTCTAAG
TGGGATGCTGCACCAAAGGCTCGTCAGGATGAGTTTGTGCGTCGTGTGATCGCCAAGGGTGTTCCATGCACTGTGCGTGA
TACCAAGGGACAAGAAATCGCTGCAGCTTGTGGACAGCTTGCTGCGGAGGAATCTGCTTAA

Upstream 100 bases:

>100_bases
TTGCTCATACAGCCATTGAACTCGTAGAATCACCGTTCCGCCACCCAAATCAGCCCAATAAGTTCAACAATTACATAACA
TCTGAAAGAATAGGGACATT

Downstream 100 bases:

>100_bases
GCCCTCAAATCTCAAAAGCCCGTTGTTTTTGCAAAAATGCGAAAACAACGGGCTTTTGTCGTTGGTGAGGTGCTCCTGAA
CATCTGTGCTTCAGCTGGTA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MATPVPLVFNAPKRGMPPTHFADLNDEARIEALKELGLPKFRLNQIARHYYGRLEADPLTMTDLPEGARQEVKDALFPTL
MSPLRVIETDDDTTQKTLWKLHDGTLLESVLMRYSDRSTLCISSQAGCGMACPFCATGQGGLDRNLSIGEIVDQVRNAAA
TMQAEGGRLSNIVFMGMGEPLANYKRVVSAVRQITQPSPAGFGISQRSVTVSTVGLAPAIRKLADEEMSVTLAVSLHTPD
DELRDTLVPVNNRWPVAEVLDAARYYADKSGRRVSIEYALIRDVNDQDWRADMLGEKLHKALGSRVHVNLIPLNPTPGSK
WDAAPKARQDEFVRRVIAKGVPCTVRDTKGQEIAAACGQLAAEESA

Sequences:

>Translated_366_residues
MATPVPLVFNAPKRGMPPTHFADLNDEARIEALKELGLPKFRLNQIARHYYGRLEADPLTMTDLPEGARQEVKDALFPTL
MSPLRVIETDDDTTQKTLWKLHDGTLLESVLMRYSDRSTLCISSQAGCGMACPFCATGQGGLDRNLSIGEIVDQVRNAAA
TMQAEGGRLSNIVFMGMGEPLANYKRVVSAVRQITQPSPAGFGISQRSVTVSTVGLAPAIRKLADEEMSVTLAVSLHTPD
DELRDTLVPVNNRWPVAEVLDAARYYADKSGRRVSIEYALIRDVNDQDWRADMLGEKLHKALGSRVHVNLIPLNPTPGSK
WDAAPKARQDEFVRRVIAKGVPCTVRDTKGQEIAAACGQLAAEESA
>Mature_365_residues
ATPVPLVFNAPKRGMPPTHFADLNDEARIEALKELGLPKFRLNQIARHYYGRLEADPLTMTDLPEGARQEVKDALFPTLM
SPLRVIETDDDTTQKTLWKLHDGTLLESVLMRYSDRSTLCISSQAGCGMACPFCATGQGGLDRNLSIGEIVDQVRNAAAT
MQAEGGRLSNIVFMGMGEPLANYKRVVSAVRQITQPSPAGFGISQRSVTVSTVGLAPAIRKLADEEMSVTLAVSLHTPDD
ELRDTLVPVNNRWPVAEVLDAARYYADKSGRRVSIEYALIRDVNDQDWRADMLGEKLHKALGSRVHVNLIPLNPTPGSKW
DAAPKARQDEFVRRVIAKGVPCTVRDTKGQEIAAACGQLAAEESA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=352, Percent_Identity=38.3522727272727, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40031; Mature: 39900

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPVPLVFNAPKRGMPPTHFADLNDEARIEALKELGLPKFRLNQIARHYYGRLEADPLT
CCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
MTDLPEGARQEVKDALFPTLMSPLRVIETDDDTTQKTLWKLHDGTLLESVLMRYSDRSTL
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEE
CISSQAGCGMACPFCATGQGGLDRNLSIGEIVDQVRNAAATMQAEGGRLSNIVFMGMGEP
EEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCH
LANYKRVVSAVRQITQPSPAGFGISQRSVTVSTVGLAPAIRKLADEEMSVTLAVSLHTPD
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCEEEEEEEEECCC
DELRDTLVPVNNRWPVAEVLDAARYYADKSGRRVSIEYALIRDVNDQDWRADMLGEKLHK
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHH
ALGSRVHVNLIPLNPTPGSKWDAAPKARQDEFVRRVIAKGVPCTVRDTKGQEIAAACGQL
HCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
AAEESA
HHCCCC
>Mature Secondary Structure 
ATPVPLVFNAPKRGMPPTHFADLNDEARIEALKELGLPKFRLNQIARHYYGRLEADPLT
CCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
MTDLPEGARQEVKDALFPTLMSPLRVIETDDDTTQKTLWKLHDGTLLESVLMRYSDRSTL
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEE
CISSQAGCGMACPFCATGQGGLDRNLSIGEIVDQVRNAAATMQAEGGRLSNIVFMGMGEP
EEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCH
LANYKRVVSAVRQITQPSPAGFGISQRSVTVSTVGLAPAIRKLADEEMSVTLAVSLHTPD
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCEEEEEEEEECCC
DELRDTLVPVNNRWPVAEVLDAARYYADKSGRRVSIEYALIRDVNDQDWRADMLGEKLHK
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHH
ALGSRVHVNLIPLNPTPGSKWDAAPKARQDEFVRRVIAKGVPCTVRDTKGQEIAAACGQL
HCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
AAEESA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]