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The map label for this gene is recA [H]

Identifier: 145295860

GI number: 145295860

Start: 1983061

End: 1984191

Strand: Reverse

Name: recA [H]

Synonym: cgR_1785

Alternate gene names: 145295860

Gene position: 1984191-1983061 (Counterclockwise)

Preceding gene: 145295861

Following gene: 145295859

Centisome position: 59.87

GC content: 53.4

Gene sequence:

>1131_bases
ATGGCTCCCAAGAAGACAGCAACAAAGGCAACTGCCGCCAAGGGGAATGATCGTCAGAAGGCACTTGATGCCGCACTAGC
CCTGATTGAGAAGGATTTCGGTAAAGGCGCTGTCATGCGTCTGGGTGATGAGAATCGTCCGCCAATCCAGACCATCTCAT
CTGGTAACACCGCGATTGATATCGCCTTGGGTATCGGTGGATTCCCACGTGGTCGAATCGTTGAGGTGTATGGCCCAGAA
TCATCAGGTAAAACCACCGTTGCACTGCACGCTATTGCGCAGGCACAAAAGGCCGGCGGCATCGCTGCATTCATTGACGC
CGAGCACGCGTTGGATCCAGATTATGCTCGCAAGCTTGGTGTAGATACTGATGCGCTTCTGGTTTCGCAGCCAGACACTG
GTGAGCAAGCACTAGAAATCGCCGACATGCTGGTTCGTTCCGGCGCAATCGACATCATCGTGATTGACTCGGTGGCTGCG
CTGACACCAAAGGCTGAAATTGAAGGCGAAATGGGCGATAGCCACGTTGGTCTTCAGGCCCGCCTCATGAGCCAGGCGCT
TCGTAAGATGACAGGTGCGCTGTACAACTCGGGTACCACCGCGATCTTCATTAACCAGCTGCGTGAAAAGATCGGTGTGA
TGTTCGGTTCCCCAGAAACCACCACCGGTGGTAAGGCCCTGAAGTTCTACGCATCTGTTCGTTGTGACATTCGACGAATC
CAGACTCTGAAGGACGGACAGGATGCCATTGGTAACCGCACCCGCTTGAAGGTCGTTAAGAACAAGGTCTCCCCACCGTT
CAAGATCGCTGAATTCGACATCATGTACGGCGAAGGCATCTCCCGTGAATCCTCCGTCATTGACTTGGCAGTGGACAACG
GCATTGTGAAGAAGTCAGGTTCCTGGTTCACCTACGAGGGTGAACAGCTTGGTCAAGGTAAGGAAAAGGTGCGTCTTTCC
CTCAAGGAGAACCCTGAACTCACCGATGAGCTGGAAGATAAGATCTTCAAGAAGCTGGGAGTAGGCAAGTACGCTGCAGC
CTCAGATGAACTCACCGACGATCCAGTAGAGCTCGTGCCTAACGTTGACTTCGATGATGAAGCCGACACCGAAGCAGACG
CTGAAGACTAA

Upstream 100 bases:

>100_bases
AGGGGTCACTGCTGCAGTGCTGTTTTAGTCAGTGCTGAAGTGATGCAACCGTAGGAATTTTCGTCCACATCAACGTCAAT
AAAACTAAGAGGAATTAAAA

Downstream 100 bases:

>100_bases
GCACCAGTTTTAACAAAGCAGGGACAATCCACACACTTAAACCATGATGTGGCTTGTTCCTGCTTTTTCGTCAACGAAGG
GCAACAACGCGATGGATATC

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAPKKTATKATAAKGNDRQKALDAALALIEKDFGKGAVMRLGDENRPPIQTISSGNTAIDIALGIGGFPRGRIVEVYGPE
SSGKTTVALHAIAQAQKAGGIAAFIDAEHALDPDYARKLGVDTDALLVSQPDTGEQALEIADMLVRSGAIDIIVIDSVAA
LTPKAEIEGEMGDSHVGLQARLMSQALRKMTGALYNSGTTAIFINQLREKIGVMFGSPETTTGGKALKFYASVRCDIRRI
QTLKDGQDAIGNRTRLKVVKNKVSPPFKIAEFDIMYGEGISRESSVIDLAVDNGIVKKSGSWFTYEGEQLGQGKEKVRLS
LKENPELTDELEDKIFKKLGVGKYAAASDELTDDPVELVPNVDFDDEADTEADAED

Sequences:

>Translated_376_residues
MAPKKTATKATAAKGNDRQKALDAALALIEKDFGKGAVMRLGDENRPPIQTISSGNTAIDIALGIGGFPRGRIVEVYGPE
SSGKTTVALHAIAQAQKAGGIAAFIDAEHALDPDYARKLGVDTDALLVSQPDTGEQALEIADMLVRSGAIDIIVIDSVAA
LTPKAEIEGEMGDSHVGLQARLMSQALRKMTGALYNSGTTAIFINQLREKIGVMFGSPETTTGGKALKFYASVRCDIRRI
QTLKDGQDAIGNRTRLKVVKNKVSPPFKIAEFDIMYGEGISRESSVIDLAVDNGIVKKSGSWFTYEGEQLGQGKEKVRLS
LKENPELTDELEDKIFKKLGVGKYAAASDELTDDPVELVPNVDFDDEADTEADAED
>Mature_375_residues
APKKTATKATAAKGNDRQKALDAALALIEKDFGKGAVMRLGDENRPPIQTISSGNTAIDIALGIGGFPRGRIVEVYGPES
SGKTTVALHAIAQAQKAGGIAAFIDAEHALDPDYARKLGVDTDALLVSQPDTGEQALEIADMLVRSGAIDIIVIDSVAAL
TPKAEIEGEMGDSHVGLQARLMSQALRKMTGALYNSGTTAIFINQLREKIGVMFGSPETTTGGKALKFYASVRCDIRRIQ
TLKDGQDAIGNRTRLKVVKNKVSPPFKIAEFDIMYGEGISRESSVIDLAVDNGIVKKSGSWFTYEGEQLGQGKEKVRLSL
KENPELTDELEDKIFKKLGVGKYAAASDELTDDPVELVPNVDFDDEADTEADAED

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=319, Percent_Identity=64.2633228840125, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 40228; Mature: 40097

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPKKTATKATAAKGNDRQKALDAALALIEKDFGKGAVMRLGDENRPPIQTISSGNTAID
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCEEE
IALGIGGFPRGRIVEVYGPESSGKTTVALHAIAQAQKAGGIAAFIDAEHALDPDYARKLG
EEEECCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHC
VDTDALLVSQPDTGEQALEIADMLVRSGAIDIIVIDSVAALTPKAEIEGEMGDSHVGLQA
CCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHH
RLMSQALRKMTGALYNSGTTAIFINQLREKIGVMFGSPETTTGGKALKFYASVRCDIRRI
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCEEECCCCCCCCCCEEEEEHHHHHHHHHH
QTLKDGQDAIGNRTRLKVVKNKVSPPFKIAEFDIMYGEGISRESSVIDLAVDNGIVKKSG
HHHHCCHHHHCCCCEEEHHHHCCCCCEEEEEEEEEECCCCCCCCCEEEEEECCCEEECCC
SWFTYEGEQLGQGKEKVRLSLKENPELTDELEDKIFKKLGVGKYAAASDELTDDPVELVP
CEEEECCHHHCCCHHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCC
NVDFDDEADTEADAED
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
APKKTATKATAAKGNDRQKALDAALALIEKDFGKGAVMRLGDENRPPIQTISSGNTAID
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCEEE
IALGIGGFPRGRIVEVYGPESSGKTTVALHAIAQAQKAGGIAAFIDAEHALDPDYARKLG
EEEECCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHC
VDTDALLVSQPDTGEQALEIADMLVRSGAIDIIVIDSVAALTPKAEIEGEMGDSHVGLQA
CCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCCCCCHHH
RLMSQALRKMTGALYNSGTTAIFINQLREKIGVMFGSPETTTGGKALKFYASVRCDIRRI
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCEEECCCCCCCCCCEEEEEHHHHHHHHHH
QTLKDGQDAIGNRTRLKVVKNKVSPPFKIAEFDIMYGEGISRESSVIDLAVDNGIVKKSG
HHHHCCHHHHCCCCEEEHHHHCCCCCEEEEEEEEEECCCCCCCCCEEEEEECCCEEECCC
SWFTYEGEQLGQGKEKVRLSLKENPELTDELEDKIFKKLGVGKYAAASDELTDDPVELVP
CEEEECCHHHCCCHHHEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCC
NVDFDDEADTEADAED
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]