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The map label for this gene is ptsI [H]

Identifier: 145295838

GI number: 145295838

Start: 1958695

End: 1960401

Strand: Reverse

Name: ptsI [H]

Synonym: cgR_1763

Alternate gene names: 145295838

Gene position: 1960401-1958695 (Counterclockwise)

Preceding gene: 145295843

Following gene: 145295834

Centisome position: 59.15

GC content: 58.7

Gene sequence:

>1707_bases
GTGGCTACTGTGGCTGATGTGAATCAAGACACTGTACTGAAGGGCACCGGCGTTGTCGGTGGAGTCCGTTATGCAAGCGC
GGTGTGGATTACCCCACGCCCCGAACTACCCCAAGCAGGCGAAGTCGTCGCCGAAGAAAACCGTGAAGCAGAGCAGGAGC
GTTTCGACGCCGCTGCAGCCACAGTCTCTTCTCGTTTGCTTGAGCGCTCCGAAGCTGCTGAAGGACCAGCAGCTGAGGTG
CTTAAAGCTACTGCTGGCATGGTCAATGACCGTGGCTGGCGTAAGGCTGTCATCAAGGGTGTCAAGGGTGGTCACCCTGC
GGAATACGCCGTGGTTGCAGCAACAACCAAGTTCATCTCCATGTTCGAAGCCGCAGGCGGCCTGATCGCAGAGCGCACCA
CAGACTTGCGAGACATCCGCGACCGCGTCATCGCAGAACTTCGTGGCGATGAAGAGCCAGGTCTGCCAGCTGTTTCCGGA
CAGGTCATTCTCTTTGCAGATGACCTCTCCCCAGCAGACACCGCGGCACTAGACACAGATCTCTTTGTGGGACTTGTCAC
TGAGCTGGGTGGCCCAACAAGCCACACCGCGATCATCGCGCGCCAGCTCAACGTCCCTTGCATCGTCGCTACCGGCGCCG
GCATCAAGGACATCAAGTCCGGCGAAAAGGTGCTTATCGACGGCAGCCTCGGCACCATTGACCGCAACGCGGACGAAGCT
GAAGCAACCAAGCTCGTCTCCGAGTCCCTCGAGCGCGCTGCTCGCATCGCCGAGTGGAAGGGTCCTGCACAAACCAAGGA
CGGCTACCGCGTTCAGCTGTTGGCCAACGTCCAAGACGGCAACTCTGCACAGCAGGCTGCACAGACCGAAGCAGAAGGCA
TCGGCCTGTTCCGCACCGAACTGTGCTTCCTTTCCGCCACCGAAGAGCCAAGCGTTGATGAGCAGGCTGCGGTCTACTCA
AAGGTGCTTGAAGCATTCCAAGAGTCCAAGGTTGTTGTCCGCTCCCTCGACGCAGGTTCTGACAAGCCAGTTCCATTCGC
ATCGATGGCTGATGAGATGAACCCAGCACTGGGTGTTCGTGGCCTGCGTATCGCACGTGGACAGGTTGATCTGCTGACTC
GCCAGCTCGACGCAATTGCGAAGGCCAGCGAAGAACTCGGCCGTGGCGACGACGCCCCAACCTGGGTTATGGCTCCAATG
GTGGCTACCGCTTATGAAGCAAAGTGGTTTGCTGACATGTGCCGTGAGCGTGGCCTAATCGCCGGCGCCATGATCGAAGT
TCCAGCAGCATCCCTGATGGCAGACAAGATCATGCCTCACCTGGACTTTGTTTCCATCGGTACCAACGACCTGACCCAGT
ACACCATGGCAGCGGACCGCATGTCTCCTGAGCTTGCCTACCTGACCGATCCTTGGCAGCCAGCAGTCCTGCGCCTGATC
AAGCACACCTGTGACGAAGGTGCTCGCTTTAATACCCCAGTCGGTGTTTGTGGTGAAGCAGCAGCAGACCCACTGTTGGC
AACTGTCCTCACCGGTCTTGGCGTGAACTCCCTGTCCGCAGCATCCACTGCTCTCGCAGCAGTCGGTGCAAAGCTGTCAG
AGGTCACCCTGGAAACCTGTAAGAAGGCAGCAGAAGCAGCACTTGACGCTGAAGGCGCAACTGAAGCACGCGATGCTGTA
CGCGCAGTGATCGACGCAGCAGTCTAA

Upstream 100 bases:

>100_bases
GATATGTGTTTGTTTGTCAATATCCAAATGTTTGAATAGTTGCACAACTGTTGATTTTGTGGTGATCTTGAGGAAATTAA
CTCAATGATTGTGAGGATGG

Downstream 100 bases:

>100_bases
ACCACTGTTGAGCTAAAAAAGCCTCAAATTCCTGTGTGGGAATTTGAGGCTTTTTGCGTGGTCTAAAGCGATTTGATGAC
CACGTGCTCCGCCCGAAGCT

Product: hypothetical protein

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MATVADVNQDTVLKGTGVVGGVRYASAVWITPRPELPQAGEVVAEENREAEQERFDAAAATVSSRLLERSEAAEGPAAEV
LKATAGMVNDRGWRKAVIKGVKGGHPAEYAVVAATTKFISMFEAAGGLIAERTTDLRDIRDRVIAELRGDEEPGLPAVSG
QVILFADDLSPADTAALDTDLFVGLVTELGGPTSHTAIIARQLNVPCIVATGAGIKDIKSGEKVLIDGSLGTIDRNADEA
EATKLVSESLERAARIAEWKGPAQTKDGYRVQLLANVQDGNSAQQAAQTEAEGIGLFRTELCFLSATEEPSVDEQAAVYS
KVLEAFQESKVVVRSLDAGSDKPVPFASMADEMNPALGVRGLRIARGQVDLLTRQLDAIAKASEELGRGDDAPTWVMAPM
VATAYEAKWFADMCRERGLIAGAMIEVPAASLMADKIMPHLDFVSIGTNDLTQYTMAADRMSPELAYLTDPWQPAVLRLI
KHTCDEGARFNTPVGVCGEAAADPLLATVLTGLGVNSLSAASTALAAVGAKLSEVTLETCKKAAEAALDAEGATEARDAV
RAVIDAAV

Sequences:

>Translated_568_residues
MATVADVNQDTVLKGTGVVGGVRYASAVWITPRPELPQAGEVVAEENREAEQERFDAAAATVSSRLLERSEAAEGPAAEV
LKATAGMVNDRGWRKAVIKGVKGGHPAEYAVVAATTKFISMFEAAGGLIAERTTDLRDIRDRVIAELRGDEEPGLPAVSG
QVILFADDLSPADTAALDTDLFVGLVTELGGPTSHTAIIARQLNVPCIVATGAGIKDIKSGEKVLIDGSLGTIDRNADEA
EATKLVSESLERAARIAEWKGPAQTKDGYRVQLLANVQDGNSAQQAAQTEAEGIGLFRTELCFLSATEEPSVDEQAAVYS
KVLEAFQESKVVVRSLDAGSDKPVPFASMADEMNPALGVRGLRIARGQVDLLTRQLDAIAKASEELGRGDDAPTWVMAPM
VATAYEAKWFADMCRERGLIAGAMIEVPAASLMADKIMPHLDFVSIGTNDLTQYTMAADRMSPELAYLTDPWQPAVLRLI
KHTCDEGARFNTPVGVCGEAAADPLLATVLTGLGVNSLSAASTALAAVGAKLSEVTLETCKKAAEAALDAEGATEARDAV
RAVIDAAV
>Mature_567_residues
ATVADVNQDTVLKGTGVVGGVRYASAVWITPRPELPQAGEVVAEENREAEQERFDAAAATVSSRLLERSEAAEGPAAEVL
KATAGMVNDRGWRKAVIKGVKGGHPAEYAVVAATTKFISMFEAAGGLIAERTTDLRDIRDRVIAELRGDEEPGLPAVSGQ
VILFADDLSPADTAALDTDLFVGLVTELGGPTSHTAIIARQLNVPCIVATGAGIKDIKSGEKVLIDGSLGTIDRNADEAE
ATKLVSESLERAARIAEWKGPAQTKDGYRVQLLANVQDGNSAQQAAQTEAEGIGLFRTELCFLSATEEPSVDEQAAVYSK
VLEAFQESKVVVRSLDAGSDKPVPFASMADEMNPALGVRGLRIARGQVDLLTRQLDAIAKASEELGRGDDAPTWVMAPMV
ATAYEAKWFADMCRERGLIAGAMIEVPAASLMADKIMPHLDFVSIGTNDLTQYTMAADRMSPELAYLTDPWQPAVLRLIK
HTCDEGARFNTPVGVCGEAAADPLLATVLTGLGVNSLSAASTALAAVGAKLSEVTLETCKKAAEAALDAEGATEARDAVR
AVIDAAV

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=437, Percent_Identity=34.5537757437071, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1789193, Length=539, Percent_Identity=31.1688311688312, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI48994992, Length=563, Percent_Identity=28.5968028419183, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1788726, Length=529, Percent_Identity=27.5992438563327, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1787994, Length=399, Percent_Identity=24.812030075188, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 59712; Mature: 59581

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVADVNQDTVLKGTGVVGGVRYASAVWITPRPELPQAGEVVAEENREAEQERFDAAAA
CCCCCCCCCCCEEECCCCCCCEEHHEEEEECCCCCCCCCCCHHHHHCHHHHHHHHHHHHH
TVSSRLLERSEAAEGPAAEVLKATAGMVNDRGWRKAVIKGVKGGHPAEYAVVAATTKFIS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHH
MFEAAGGLIAERTTDLRDIRDRVIAELRGDEEPGLPAVSGQVILFADDLSPADTAALDTD
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
LFVGLVTELGGPTSHTAIIARQLNVPCIVATGAGIKDIKSGEKVLIDGSLGTIDRNADEA
HHHHHHHHCCCCCCHHHHEEEECCCCEEEECCCCHHHHCCCCEEEEECCCCCCCCCCCHH
EATKLVSESLERAARIAEWKGPAQTKDGYRVQLLANVQDGNSAQQAAQTEAEGIGLFRTE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHH
LCFLSATEEPSVDEQAAVYSKVLEAFQESKVVVRSLDAGSDKPVPFASMADEMNPALGVR
EEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHCCHHHCCC
GLRIARGQVDLLTRQLDAIAKASEELGRGDDAPTWVMAPMVATAYEAKWFADMCRERGLI
CEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
AGAMIEVPAASLMADKIMPHLDFVSIGTNDLTQYTMAADRMSPELAYLTDPWQPAVLRLI
EEHHHHCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
KHTCDEGARFNTPVGVCGEAAADPLLATVLTGLGVNSLSAASTALAAVGAKLSEVTLETC
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KKAAEAALDAEGATEARDAVRAVIDAAV
HHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATVADVNQDTVLKGTGVVGGVRYASAVWITPRPELPQAGEVVAEENREAEQERFDAAAA
CCCCCCCCCCEEECCCCCCCEEHHEEEEECCCCCCCCCCCHHHHHCHHHHHHHHHHHHH
TVSSRLLERSEAAEGPAAEVLKATAGMVNDRGWRKAVIKGVKGGHPAEYAVVAATTKFIS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHH
MFEAAGGLIAERTTDLRDIRDRVIAELRGDEEPGLPAVSGQVILFADDLSPADTAALDTD
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
LFVGLVTELGGPTSHTAIIARQLNVPCIVATGAGIKDIKSGEKVLIDGSLGTIDRNADEA
HHHHHHHHCCCCCCHHHHEEEECCCCEEEECCCCHHHHCCCCEEEEECCCCCCCCCCCHH
EATKLVSESLERAARIAEWKGPAQTKDGYRVQLLANVQDGNSAQQAAQTEAEGIGLFRTE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHH
LCFLSATEEPSVDEQAAVYSKVLEAFQESKVVVRSLDAGSDKPVPFASMADEMNPALGVR
EEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHCCHHHCCC
GLRIARGQVDLLTRQLDAIAKASEELGRGDDAPTWVMAPMVATAYEAKWFADMCRERGLI
CEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
AGAMIEVPAASLMADKIMPHLDFVSIGTNDLTQYTMAADRMSPELAYLTDPWQPAVLRLI
EEHHHHCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
KHTCDEGARFNTPVGVCGEAAADPLLATVLTGLGVNSLSAASTALAAVGAKLSEVTLETC
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KKAAEAALDAEGATEARDAVRAVIDAAV
HHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]