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The map label for this gene is folD

Identifier: 145294816

GI number: 145294816

Start: 842118

End: 842972

Strand: Direct

Name: folD

Synonym: cgR_0763

Alternate gene names: 145294816

Gene position: 842118-842972 (Clockwise)

Preceding gene: 145294814

Following gene: 145294817

Centisome position: 25.41

GC content: 57.08

Gene sequence:

>855_bases
GTGACTGCAATCAAACTTGATGGAAACTTATACCGCGGGGAAATTTTCGCCGACTTGGAACAGCGCGTTGCTGCGTTGAA
GGAGAAAGGGATTGTGCCGGGGCTTGCCACCGTGCTGGTGGGTGATGACCCAGCGAGCCACTCTTACGTGAAGATGAAGC
ATCGTGACTGTGAGCAGATTGGTGTGAACTCGATCCGTAAGGATCTGCCTGCTGATGTCACGCAGGAAGAGCTTTTCGCT
GTCATCGATGAACTGAACAACGATGATTCTTGCACTGGTTACATTGTGCAGCTTCCTTTGCCTAAGCACTTGGATGAAAA
CGCTGTGCTGGAGCGCATTGATCCAGCTAAGGATGCTGATGGCCTGCACCCTGTAAACCTGGGCAAGCTTGTGCTCAACG
AGCCAGCTCCACTGCCATGCACCCCGAATGGTTCCATCAGTTTGTTGCGTCGTTTCGGCGTTGAGCTTGATGGCGCGAAG
GTTGTTGTCATTGGCCGTGGCGTCACCGTTGGTCGCCCAATTGGCCTGATGCTGACCCGCCGTTCCGAGAACTCCACCGT
CACCTTGTGCCACACTGGCACGAAGGATCTGGCTGCGGAGACCCGTGCGGCTGACGTCATCATTGCTGCAGCTGGTCAGC
CGCACATGCTGACCGCAGACATGGTCAAGCCAGGCGCAGCGGTGCTCGATGTCGGCGTCTCCCGCAAGGACGGCAAGTTG
CTTGGCGACGTCCACCCCGACGTGTGGGAAGTCGCCGGCGCGGTCTCACCAAACCCTGGAGGAGTCGGTCCTCTGACCCG
TGCATTCTTGGTGCACAATGTTGTCGAGCGCGCTGAAAAGCTGGCTGGACTCTAA

Upstream 100 bases:

>100_bases
TGTTGGGAGGGATGACAGGATTGTCGAAGATAACGTGAAGTGGGTGTTCCGGCATGTGTTTGATTGTAAGGCCTTGGAAA
AGGGTGGAATAATAGCGGGC

Downstream 100 bases:

>100_bases
AAACAGATGACTAATCCCGGCAAAAAGCACATCAGTGAAGAGCAACTTCTGGCGAATCCCCATGACGGTGGATTAGCCCC
ATCGAAGTTTCCCATCAAGG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTAIKLDGNLYRGEIFADLEQRVAALKEKGIVPGLATVLVGDDPASHSYVKMKHRDCEQIGVNSIRKDLPADVTQEELFA
VIDELNNDDSCTGYIVQLPLPKHLDENAVLERIDPAKDADGLHPVNLGKLVLNEPAPLPCTPNGSISLLRRFGVELDGAK
VVVIGRGVTVGRPIGLMLTRRSENSTVTLCHTGTKDLAAETRAADVIIAAAGQPHMLTADMVKPGAAVLDVGVSRKDGKL
LGDVHPDVWEVAGAVSPNPGGVGPLTRAFLVHNVVERAEKLAGL

Sequences:

>Translated_284_residues
MTAIKLDGNLYRGEIFADLEQRVAALKEKGIVPGLATVLVGDDPASHSYVKMKHRDCEQIGVNSIRKDLPADVTQEELFA
VIDELNNDDSCTGYIVQLPLPKHLDENAVLERIDPAKDADGLHPVNLGKLVLNEPAPLPCTPNGSISLLRRFGVELDGAK
VVVIGRGVTVGRPIGLMLTRRSENSTVTLCHTGTKDLAAETRAADVIIAAAGQPHMLTADMVKPGAAVLDVGVSRKDGKL
LGDVHPDVWEVAGAVSPNPGGVGPLTRAFLVHNVVERAEKLAGL
>Mature_283_residues
TAIKLDGNLYRGEIFADLEQRVAALKEKGIVPGLATVLVGDDPASHSYVKMKHRDCEQIGVNSIRKDLPADVTQEELFAV
IDELNNDDSCTGYIVQLPLPKHLDENAVLERIDPAKDADGLHPVNLGKLVLNEPAPLPCTPNGSISLLRRFGVELDGAKV
VVIGRGVTVGRPIGLMLTRRSENSTVTLCHTGTKDLAAETRAADVIIAAAGQPHMLTADMVKPGAAVLDVGVSRKDGKLL
GDVHPDVWEVAGAVSPNPGGVGPLTRAFLVHNVVERAEKLAGL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=264, Percent_Identity=42.8030303030303, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI94721354, Length=288, Percent_Identity=41.3194444444444, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=37.1134020618557, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI36796743, Length=231, Percent_Identity=26.8398268398268, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=42.2939068100358, Blast_Score=211, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17568735, Length=294, Percent_Identity=36.734693877551, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319558, Length=274, Percent_Identity=41.970802919708, Blast_Score=202, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6321643, Length=295, Percent_Identity=36.271186440678, Blast_Score=176, Evalue=3e-45,
Organism=Drosophila melanogaster, GI17136816, Length=291, Percent_Identity=41.5807560137457, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17136818, Length=291, Percent_Identity=41.5807560137457, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=38.0622837370242, Blast_Score=187, Evalue=8e-48,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=38.0622837370242, Blast_Score=187, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=38.0622837370242, Blast_Score=187, Evalue=8e-48,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=38.0622837370242, Blast_Score=187, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_CORGB (A4QBY8)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001137637.1
- ProteinModelPortal:   A4QBY8
- SMR:   A4QBY8
- STRING:   A4QBY8
- GeneID:   4992810
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_0763
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   PVSCTPR
- ProtClustDB:   PRK14193
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30150; Mature: 30019

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIKLDGNLYRGEIFADLEQRVAALKEKGIVPGLATVLVGDDPASHSYVKMKHRDCEQI
CEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCCCHHHH
GVNSIRKDLPADVTQEELFAVIDELNNDDSCTGYIVQLPLPKHLDENAVLERIDPAKDAD
CHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCC
GLHPVNLGKLVLNEPAPLPCTPNGSISLLRRFGVELDGAKVVVIGRGVTVGRPIGLMLTR
CCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEE
RSENSTVTLCHTGTKDLAAETRAADVIIAAAGQPHMLTADMVKPGAAVLDVGVSRKDGKL
CCCCCEEEEEECCCHHHHHHHHHCEEEEEECCCCCEEEHHHCCCCCEEEEECCCCCCCCE
LGDVHPDVWEVAGAVSPNPGGVGPLTRAFLVHNVVERAEKLAGL
ECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAIKLDGNLYRGEIFADLEQRVAALKEKGIVPGLATVLVGDDPASHSYVKMKHRDCEQI
EEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCCCHHHH
GVNSIRKDLPADVTQEELFAVIDELNNDDSCTGYIVQLPLPKHLDENAVLERIDPAKDAD
CHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCC
GLHPVNLGKLVLNEPAPLPCTPNGSISLLRRFGVELDGAKVVVIGRGVTVGRPIGLMLTR
CCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEE
RSENSTVTLCHTGTKDLAAETRAADVIIAAAGQPHMLTADMVKPGAAVLDVGVSRKDGKL
CCCCCEEEEEECCCHHHHHHHHHCEEEEEECCCCCEEEHHHCCCCCEEEEECCCCCCCCE
LGDVHPDVWEVAGAVSPNPGGVGPLTRAFLVHNVVERAEKLAGL
ECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA