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The map label for this gene is gltD [H]

Identifier: 145294309

GI number: 145294309

Start: 289481

End: 291001

Strand: Direct

Name: gltD [H]

Synonym: cgR_0264

Alternate gene names: 145294309

Gene position: 289481-291001 (Clockwise)

Preceding gene: 145294308

Following gene: 145294316

Centisome position: 8.73

GC content: 60.22

Gene sequence:

>1521_bases
ATGGCCGACCCACAAGGATTCATCAAATACTCCCGACGCGAGCCTGCACACCGCCCGGTCCCGCTGCGCCTCATGGACTA
CTCCGAGGTCTACGAAAAGGCACCGGCAGGTCAGATCGAGGAACAGGCTGCCCGCTGCATGGATTGCGGTGTCCCGTTCT
GCCACGAAGGCTGCCCACTGGGCAACATCATCCCTGAGTGGAATGATCTGGTACGCCAAGGTCGGTGGAAGGAAGCCTAC
GATCGCCTGCACGCGACCAACAATTTCCCCGAGTTCACCGGCCGTTTGTGCCCCGCACCCTGCGAAGGCGCTTGTGTGCT
CGGCATCAACGATGATTCTGTCACCATCAAAAACGTTGAGCTGGAAATCGTCGAAAAGGCATTCCGCGAAGGCTGGGTGC
AACCAGTAATCCCATCCATGTCCACCGGCCTATCCGTCGCCGTCGTTGGTTCCGGCCCCGCAGGTCTAGCCGCCGCACAG
CAGCTCACCCGCGCAGGCCACAGCGTTACCGTCTTTGAACGCGATGACCGCCTCGGCGGCCTCATGCGCTACGGCGTGCC
AGAATACAAAATGGAAAACCGCTGGATCGACCGCCGCATCGAGCAAATGGAAGCAGAGGGCACAACTTTCCAGGTGGGCA
CCTCGCCCCGCGCCGCTGAACTAGCGCTTTTCGACGCGATCCTCCTCGCAACCGGCACCCCAGTGGCCCGCGAACTCTCA
GTTCCAGGCGACGATCTCAACGGCATCCATGCGGCAATGGATTACCTCACCGCCCAAAACCGCATCAACGAAGGCGACGG
TGACGTTTCTCCCATCAACGCCAAAGGTAAGAAAGTTGTCATCATCGGTGGCGGCGACACCGGCACCGACTGCTTCGGTA
CCGCACTGCGCCAAGGTGCAGAATCAGTCACCCAATTTGATATCCGCCCCCGCGCACCTTTCCAACGTGCCGATTCCACC
CCATGGCCGATGTACCCAAACCTCTTCCGCACCGCAACGGCTCACGAAGAAGGCGAATACATCATCACCGGCGACGAATC
AGCCGATGAAATTGCAGCCCTAGGCCTTGCCGAACGTGCCGCAGGCTCCACGCTTGGTGAACGTAAATTCGCCGTCAACA
CCGTGGAATTCCACGGCAACAACGGCCACGTCACCGGACTCACCGGCAACCAAATCCGCGTTGTCAACGGCAAACGTGAA
CCAATCGAAGGCACCGAATTCCCCTTCGAAGCAGACCTCGTTCTCGTCGCACTCGGATTCACCGGCGCGGAACAAGGCGG
ATTGGCACACGAACTAGGCGTAGGTTTCGACGACCGCGGCCGCATCCTCCGCGACTCCGAATACCGCAGCCCCACCAACT
CTCGCGTTTACATCGCAGGCGATAACGGCCGTGGCCAGTCCCTGATTGTATGGGCAATTGCTGAAGGCCGCGCATGCGCC
GCAGCTATCGACGCCGACCTCATGGGTGAAACTGCACTTCCAGTAGCAGTTGCACCACAGGACGTGCCGCTGGCTGTCTA
G

Upstream 100 bases:

>100_bases
AAATCATGCCGCGCGATTTCCAAAAAGTACTCAACATCATCGAAACGGCCCACGCTGAGGGCCAAGACCCAGCAATCAAG
ATCATGGAGGCAGTGAGCTA

Downstream 100 bases:

>100_bases
GAGGATTGGCGTCAAAAACCACTCGAAACTGCAGTTTCGAGTGGTTTTGCGTATTCAGAATTTAAGGAAATGCTTCAAAA
CTATCCATATTTTTGAAGCG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MADPQGFIKYSRREPAHRPVPLRLMDYSEVYEKAPAGQIEEQAARCMDCGVPFCHEGCPLGNIIPEWNDLVRQGRWKEAY
DRLHATNNFPEFTGRLCPAPCEGACVLGINDDSVTIKNVELEIVEKAFREGWVQPVIPSMSTGLSVAVVGSGPAGLAAAQ
QLTRAGHSVTVFERDDRLGGLMRYGVPEYKMENRWIDRRIEQMEAEGTTFQVGTSPRAAELALFDAILLATGTPVARELS
VPGDDLNGIHAAMDYLTAQNRINEGDGDVSPINAKGKKVVIIGGGDTGTDCFGTALRQGAESVTQFDIRPRAPFQRADST
PWPMYPNLFRTATAHEEGEYIITGDESADEIAALGLAERAAGSTLGERKFAVNTVEFHGNNGHVTGLTGNQIRVVNGKRE
PIEGTEFPFEADLVLVALGFTGAEQGGLAHELGVGFDDRGRILRDSEYRSPTNSRVYIAGDNGRGQSLIVWAIAEGRACA
AAIDADLMGETALPVAVAPQDVPLAV

Sequences:

>Translated_506_residues
MADPQGFIKYSRREPAHRPVPLRLMDYSEVYEKAPAGQIEEQAARCMDCGVPFCHEGCPLGNIIPEWNDLVRQGRWKEAY
DRLHATNNFPEFTGRLCPAPCEGACVLGINDDSVTIKNVELEIVEKAFREGWVQPVIPSMSTGLSVAVVGSGPAGLAAAQ
QLTRAGHSVTVFERDDRLGGLMRYGVPEYKMENRWIDRRIEQMEAEGTTFQVGTSPRAAELALFDAILLATGTPVARELS
VPGDDLNGIHAAMDYLTAQNRINEGDGDVSPINAKGKKVVIIGGGDTGTDCFGTALRQGAESVTQFDIRPRAPFQRADST
PWPMYPNLFRTATAHEEGEYIITGDESADEIAALGLAERAAGSTLGERKFAVNTVEFHGNNGHVTGLTGNQIRVVNGKRE
PIEGTEFPFEADLVLVALGFTGAEQGGLAHELGVGFDDRGRILRDSEYRSPTNSRVYIAGDNGRGQSLIVWAIAEGRACA
AAIDADLMGETALPVAVAPQDVPLAV
>Mature_505_residues
ADPQGFIKYSRREPAHRPVPLRLMDYSEVYEKAPAGQIEEQAARCMDCGVPFCHEGCPLGNIIPEWNDLVRQGRWKEAYD
RLHATNNFPEFTGRLCPAPCEGACVLGINDDSVTIKNVELEIVEKAFREGWVQPVIPSMSTGLSVAVVGSGPAGLAAAQQ
LTRAGHSVTVFERDDRLGGLMRYGVPEYKMENRWIDRRIEQMEAEGTTFQVGTSPRAAELALFDAILLATGTPVARELSV
PGDDLNGIHAAMDYLTAQNRINEGDGDVSPINAKGKKVVIIGGGDTGTDCFGTALRQGAESVTQFDIRPRAPFQRADSTP
WPMYPNLFRTATAHEEGEYIITGDESADEIAALGLAERAAGSTLGERKFAVNTVEFHGNNGHVTGLTGNQIRVVNGKREP
IEGTEFPFEADLVLVALGFTGAEQGGLAHELGVGFDDRGRILRDSEYRSPTNSRVYIAGDNGRGQSLIVWAIAEGRACAA
AIDADLMGETALPVAVAPQDVPLAV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=298, Percent_Identity=24.496644295302, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1789606, Length=498, Percent_Identity=33.9357429718875, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1788811, Length=494, Percent_Identity=33.4008097165992, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87082180, Length=481, Percent_Identity=30.1455301455301, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1789243, Length=289, Percent_Identity=31.1418685121107, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788468, Length=272, Percent_Identity=27.2058823529412, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1789463, Length=210, Percent_Identity=30, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17570289, Length=509, Percent_Identity=43.2220039292731, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71984108, Length=291, Percent_Identity=22.680412371134, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=507, Percent_Identity=41.6173570019724, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI28574881, Length=505, Percent_Identity=46.1386138613861, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665539, Length=505, Percent_Identity=46.1386138613861, Blast_Score=440, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665547, Length=505, Percent_Identity=46.1386138613861, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24665543, Length=505, Percent_Identity=46.1386138613861, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24640763, Length=329, Percent_Identity=25.531914893617, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI18858217, Length=329, Percent_Identity=25.531914893617, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54565; Mature: 54434

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPQGFIKYSRREPAHRPVPLRLMDYSEVYEKAPAGQIEEQAARCMDCGVPFCHEGCPL
CCCCHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCCC
GNIIPEWNDLVRQGRWKEAYDRLHATNNFPEFTGRLCPAPCEGACVLGINDDSVTIKNVE
CCCCCCHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEECCCCEEEEECH
LEIVEKAFREGWVQPVIPSMSTGLSVAVVGSGPAGLAAAQQLTRAGHSVTVFERDDRLGG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
LMRYGVPEYKMENRWIDRRIEQMEAEGTTFQVGTSPRAAELALFDAILLATGTPVARELS
HHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEC
VPGDDLNGIHAAMDYLTAQNRINEGDGDVSPINAKGKKVVIIGGGDTGTDCFGTALRQGA
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
ESVTQFDIRPRAPFQRADSTPWPMYPNLFRTATAHEEGEYIITGDESADEIAALGLAERA
HHHHEECCCCCCCHHCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHH
AGSTLGERKFAVNTVEFHGNNGHVTGLTGNQIRVVNGKREPIEGTEFPFEADLVLVALGF
CCCCCCCCEEEEEEEEEECCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEC
TGAEQGGLAHELGVGFDDRGRILRDSEYRSPTNSRVYIAGDNGRGQSLIVWAIAEGRACA
CCCCCCCCHHHHCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCEEE
AAIDADLMGETALPVAVAPQDVPLAV
EHHCHHHCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
ADPQGFIKYSRREPAHRPVPLRLMDYSEVYEKAPAGQIEEQAARCMDCGVPFCHEGCPL
CCCHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCCC
GNIIPEWNDLVRQGRWKEAYDRLHATNNFPEFTGRLCPAPCEGACVLGINDDSVTIKNVE
CCCCCCHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEECCCCEEEEECH
LEIVEKAFREGWVQPVIPSMSTGLSVAVVGSGPAGLAAAQQLTRAGHSVTVFERDDRLGG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCC
LMRYGVPEYKMENRWIDRRIEQMEAEGTTFQVGTSPRAAELALFDAILLATGTPVARELS
HHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEC
VPGDDLNGIHAAMDYLTAQNRINEGDGDVSPINAKGKKVVIIGGGDTGTDCFGTALRQGA
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
ESVTQFDIRPRAPFQRADSTPWPMYPNLFRTATAHEEGEYIITGDESADEIAALGLAERA
HHHHEECCCCCCCHHCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHH
AGSTLGERKFAVNTVEFHGNNGHVTGLTGNQIRVVNGKREPIEGTEFPFEADLVLVALGF
CCCCCCCCEEEEEEEEEECCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCEEEEEEEC
TGAEQGGLAHELGVGFDDRGRILRDSEYRSPTNSRVYIAGDNGRGQSLIVWAIAEGRACA
CCCCCCCCHHHHCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCEEE
AAIDADLMGETALPVAVAPQDVPLAV
EHHCHHHCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]