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The map label for this gene is crr [H]

Identifier: 138897007

GI number: 138897007

Start: 3485223

End: 3485717

Strand: Reverse

Name: crr [H]

Synonym: GTNG_3378

Alternate gene names: 138897007

Gene position: 3485717-3485223 (Counterclockwise)

Preceding gene: 138897008

Following gene: 138897006

Centisome position: 98.18

GC content: 52.93

Gene sequence:

>495_bases
TTGTTCAAAAAATGGTTTGGCAAAAAACAGTCAGAGGAAGAAACGATTGTTGCGCCGCTGAGTGGGACGGTCGTGCCACT
TGAGGACGTGCCTGATCCGGTGTTTGCGCAAAAAATGATGGGCGACGGTATCGCCATTGATCCAACGGACGGGGACGTTG
TCGCTCCAGTGGATGGCGAAGTCATTCAGCTTTTTCCGACGAAACATGCCGTTGGCTTGCGCTCGGAAGCAGGGGTTGAG
CTGCTTATTCATGTCGGCATCGATACGGTGGCGATGAACGGAGAAGGGTTTACCGCCCATGTACAGGCCGGCGACCGGGT
GAAACGAGGCGAGCGGCTCCTTTCTGTTGACTTCTCGCTCGTTCGTGAAAAGGCGAAAAGCGCCATTACGCCGATCATCA
TTACGAACGGCGACGCGCTTGCTACCCTTGAGAAAAAGGCTGAAGGCTCGGCGACAAAAGGGGAGACCGTCTTATTTCAT
GTAAAAATGAAATAA

Upstream 100 bases:

>100_bases
ATGCTTGACCCGTCAGAGCAGCAAGCGGCGCGGTTTGCAGTTTCCTTCCATTCGGGTATACTTGTTGTCGGAAGAACTGA
CAAAGAAAGGATGACATACG

Downstream 100 bases:

>100_bases
CATTCACCGCCCGGTTTTCCTCACATCAGGATAACCAGCCGGTTGGAATTGGCACTCACGGGTATCTTCATATGGATATC
GAGAAATGGGAACGCTAAAA

Product: PTS system glucose-specific IIA

Products: Protein Histidine; Sugar Phosphate.; glucose-6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MFKKWFGKKQSEEETIVAPLSGTVVPLEDVPDPVFAQKMMGDGIAIDPTDGDVVAPVDGEVIQLFPTKHAVGLRSEAGVE
LLIHVGIDTVAMNGEGFTAHVQAGDRVKRGERLLSVDFSLVREKAKSAITPIIITNGDALATLEKKAEGSATKGETVLFH
VKMK

Sequences:

>Translated_164_residues
MFKKWFGKKQSEEETIVAPLSGTVVPLEDVPDPVFAQKMMGDGIAIDPTDGDVVAPVDGEVIQLFPTKHAVGLRSEAGVE
LLIHVGIDTVAMNGEGFTAHVQAGDRVKRGERLLSVDFSLVREKAKSAITPIIITNGDALATLEKKAEGSATKGETVLFH
VKMK
>Mature_164_residues
MFKKWFGKKQSEEETIVAPLSGTVVPLEDVPDPVFAQKMMGDGIAIDPTDGDVVAPVDGEVIQLFPTKHAVGLRSEAGVE
LLIHVGIDTVAMNGEGFTAHVQAGDRVKRGERLLSVDFSLVREKAKSAITPIIITNGDALATLEKKAEGSATKGETVLFH
VKMK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788757, Length=155, Percent_Identity=49.6774193548387, Blast_Score=148, Evalue=2e-37,
Organism=Escherichia coli, GI1790159, Length=147, Percent_Identity=43.5374149659864, Blast_Score=130, Evalue=3e-32,
Organism=Escherichia coli, GI1786894, Length=147, Percent_Identity=41.4965986394558, Blast_Score=129, Evalue=7e-32,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 17550; Mature: 17550

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKWFGKKQSEEETIVAPLSGTVVPLEDVPDPVFAQKMMGDGIAIDPTDGDVVAPVDGE
CCCCCCCCCCCCCCEEEEECCCCEEECCCCCCHHHHHHHHCCCEEECCCCCCEEECCCCC
VIQLFPTKHAVGLRSEAGVELLIHVGIDTVAMNGEGFTAHVQAGDRVKRGERLLSVDFSL
EEEEECCCCCCCCCCCCCEEEEEEECCEEEEECCCCEEEEECCCCCHHCCCEEEEHHHHH
VREKAKSAITPIIITNGDALATLEKKAEGSATKGETVLFHVKMK
HHHHHHHCCCCEEEECCCEEEEEECCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MFKKWFGKKQSEEETIVAPLSGTVVPLEDVPDPVFAQKMMGDGIAIDPTDGDVVAPVDGE
CCCCCCCCCCCCCCEEEEECCCCEEECCCCCCHHHHHHHHCCCEEECCCCCCEEECCCCC
VIQLFPTKHAVGLRSEAGVELLIHVGIDTVAMNGEGFTAHVQAGDRVKRGERLLSVDFSL
EEEEECCCCCCCCCCCCCEEEEEEECCEEEEECCCCEEEEECCCCCHHCCCEEEEHHHHH
VREKAKSAITPIIITNGDALATLEKKAEGSATKGETVLFHVKMK
HHHHHHHCCCCEEEECCCEEEEEECCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar; phosphoenolpyruvate; beta-D-glucose [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm] [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytop

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]