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The map label for this gene is ywkE [H]

Identifier: 138896953

GI number: 138896953

Start: 3428074

End: 3428940

Strand: Reverse

Name: ywkE [H]

Synonym: GTNG_3322

Alternate gene names: 138896953

Gene position: 3428940-3428074 (Counterclockwise)

Preceding gene: 138896954

Following gene: 138896952

Centisome position: 96.58

GC content: 55.94

Gene sequence:

>867_bases
ATGCGTCGTAACGTACATGAGGTCCTCACCTGGGCTTCTTCTTTTTTGCATGCTCATGGAAAGGAAGAGCGGGCGGCCGA
GTGGCTATTGTGTCACCATTTGGGCACTGACAGAGCCGGGTTGTTCGCCCGTTGGCGTGAGCCGGTTGAGGAAACGGTGT
ATGAACGATTCGCAGCCGATGTGCGCCGCCATGCCGTAGACCATGTGCCGATTCAATATTTGATCGGCCATGAATGGTTT
TACGGCCGACGGTTCCTTGTAAACCGCCATGTGTTAATCCCGCGACCGGAGACAGAAGAGCTTGTGCTTGGTGTCTTAGA
GCGTCTTCCTCGTCTGTTTGAGGGACGGGAGCGGATTGATGTCGTCGATGTAGGCACTGGCAGCGGGGCGATTGCCGTGA
CGTTGGCGCTTGAGAATCGCACGCTTTCAGTGACCGCGACTGATATTTCCGAAGAAGCGCTCACAGTCGCCCGGGAAAAT
GCCGAGCGGCTTGGGGCTCGCATTTCGTTTTTGCGCGGTGACTTATTGCAGCCGCTCATTGAGCAAGGGCAGACAGTCGA
TGTTGTCGTTTCAAATCCGCCGTACATTCCCGAGACTGATGCCGCTACACTCTCTCCGGTCGTGAAAGACTATGAGCCGC
ACACGGCACTTTTTGGCGGCCGTGACGGGCTTGATTTTTACCGCCGCTTCGCTCGCGACTTGCCGCTTGTGCTCGGTACC
CCAGCGCTTGCCGCGTTTGAAGTCGGCGCCGGACAAGGAGAAGCGGTCGCCTCGCTCTTAGCAGCGGCGTTTCCTGAGGC
TGAGATCGAAGTGGCTTTTGACTTGAACGGCAAAGACCGAATGGTCTATATGACAAGAAAAAATTAG

Upstream 100 bases:

>100_bases
ACGATTCAAAAGCTTGACCTCGTGTTAGACGGGCAGCTCGATGAAATTATCGAGGCGCTCATTTTAGACGACCAGTCGAA
AAAACTGGAGCAAGCGAACG

Downstream 100 bases:

>100_bases
ATTCATAGGTTTAAAATGAGGCGGCGCCGTCCACAATGAATGTTGAAAGGACGGTGCCGATAATGGTGATCAGGGGAAAT
TCCATCGCTGTATGTTTATA

Product: HemK family modification methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRRNVHEVLTWASSFLHAHGKEERAAEWLLCHHLGTDRAGLFARWREPVEETVYERFAADVRRHAVDHVPIQYLIGHEWF
YGRRFLVNRHVLIPRPETEELVLGVLERLPRLFEGRERIDVVDVGTGSGAIAVTLALENRTLSVTATDISEEALTVAREN
AERLGARISFLRGDLLQPLIEQGQTVDVVVSNPPYIPETDAATLSPVVKDYEPHTALFGGRDGLDFYRRFARDLPLVLGT
PALAAFEVGAGQGEAVASLLAAAFPEAEIEVAFDLNGKDRMVYMTRKN

Sequences:

>Translated_288_residues
MRRNVHEVLTWASSFLHAHGKEERAAEWLLCHHLGTDRAGLFARWREPVEETVYERFAADVRRHAVDHVPIQYLIGHEWF
YGRRFLVNRHVLIPRPETEELVLGVLERLPRLFEGRERIDVVDVGTGSGAIAVTLALENRTLSVTATDISEEALTVAREN
AERLGARISFLRGDLLQPLIEQGQTVDVVVSNPPYIPETDAATLSPVVKDYEPHTALFGGRDGLDFYRRFARDLPLVLGT
PALAAFEVGAGQGEAVASLLAAAFPEAEIEVAFDLNGKDRMVYMTRKN
>Mature_288_residues
MRRNVHEVLTWASSFLHAHGKEERAAEWLLCHHLGTDRAGLFARWREPVEETVYERFAADVRRHAVDHVPIQYLIGHEWF
YGRRFLVNRHVLIPRPETEELVLGVLERLPRLFEGRERIDVVDVGTGSGAIAVTLALENRTLSVTATDISEEALTVAREN
AERLGARISFLRGDLLQPLIEQGQTVDVVVSNPPYIPETDAATLSPVVKDYEPHTALFGGRDGLDFYRRFARDLPLVLGT
PALAAFEVGAGQGEAVASLLAAAFPEAEIEVAFDLNGKDRMVYMTRKN

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=280, Percent_Identity=29.6428571428571, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1787463, Length=246, Percent_Identity=33.739837398374, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082085, Length=239, Percent_Identity=30.5439330543933, Blast_Score=92, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324266, Length=162, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24582226, Length=167, Percent_Identity=33.5329341317365, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 32229; Mature: 32229

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNVHEVLTWASSFLHAHGKEERAAEWLLCHHLGTDRAGLFARWREPVEETVYERFAAD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VRRHAVDHVPIQYLIGHEWFYGRRFLVNRHVLIPRPETEELVLGVLERLPRLFEGRERID
HHHHHHCCCCHHHHHCCCHHHCCEEEEECEEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
VVDVGTGSGAIAVTLALENRTLSVTATDISEEALTVARENAERLGARISFLRGDLLQPLI
EEEECCCCCEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGQTVDVVVSNPPYIPETDAATLSPVVKDYEPHTALFGGRDGLDFYRRFARDLPLVLGT
HCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCEEECC
PALAAFEVGAGQGEAVASLLAAAFPEAEIEVAFDLNGKDRMVYMTRKN
CCHHEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECC
>Mature Secondary Structure
MRRNVHEVLTWASSFLHAHGKEERAAEWLLCHHLGTDRAGLFARWREPVEETVYERFAAD
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VRRHAVDHVPIQYLIGHEWFYGRRFLVNRHVLIPRPETEELVLGVLERLPRLFEGRERID
HHHHHHCCCCHHHHHCCCHHHCCEEEEECEEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
VVDVGTGSGAIAVTLALENRTLSVTATDISEEALTVARENAERLGARISFLRGDLLQPLI
EEEECCCCCEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQGQTVDVVVSNPPYIPETDAATLSPVVKDYEPHTALFGGRDGLDFYRRFARDLPLVLGT
HCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCEEECC
PALAAFEVGAGQGEAVASLLAAAFPEAEIEVAFDLNGKDRMVYMTRKN
CCHHEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]