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The map label for this gene is hpaC [H]

Identifier: 138896610

GI number: 138896610

Start: 3070147

End: 3070626

Strand: Reverse

Name: hpaC [H]

Synonym: GTNG_2973

Alternate gene names: 138896610

Gene position: 3070626-3070147 (Counterclockwise)

Preceding gene: 138896611

Following gene: 138896608

Centisome position: 86.49

GC content: 51.46

Gene sequence:

>480_bases
ATGGGAGAACCGATGGATGATCGTACATTTCGCAACGCGATGGGGAAATTCGCAACTGGTGTGACGGTCGTGACGACCGA
ATTTCAAGGGGAAGTGAAAGGGATGACGGCGAACGCGTTTATGTCCGTTTCGCTTGACCCGAAGCTGGTGGTCGTCTCGA
TTGGCCATAAAGCGCGGATGCATGACATCGTCAAGCAAACCGGGAAGTTTGCGGTCAATCTTTTGACACGCGACCAAGAA
GACTTGTCGCGTTTGTTTGCGGGTCAGCTGAAGGAAGAACGGGATGTGGCGTTTGAATGGGTGGACGGCCACCCGATTTT
GCCGGATGCGATGGCGAATGTGTTATGCCGTGTGCACAGCACGTATGTCGCCGGCGACCATACGCTTTATTTCGGCGAAG
TGACCCATATTGTGATGAAAGATGAACCAGGCGATCCGCTCTTATTTTTTGAAGGAAAATATCGAAGCATTGGCCAATAA

Upstream 100 bases:

>100_bases
CACAGTGATTTTTCATCGGCCTGTTGGGAGCAGCAAACGTCAAGCTGTATGAGCGTTGCTTTATCACAAAAGAGAACAAC
GAGATGATGAAAAGGGAAGG

Downstream 100 bases:

>100_bases
GGATGAGGCAGGTGTCTGGCAGCTAGCCGAGGCATCTGTTATTCGGTTCATATGAGAGTAAAGGGTGTCCCAAAATAACG
GGACACCCTTTTTCATCGTC

Product: flavin reductase

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MGEPMDDRTFRNAMGKFATGVTVVTTEFQGEVKGMTANAFMSVSLDPKLVVVSIGHKARMHDIVKQTGKFAVNLLTRDQE
DLSRLFAGQLKEERDVAFEWVDGHPILPDAMANVLCRVHSTYVAGDHTLYFGEVTHIVMKDEPGDPLLFFEGKYRSIGQ

Sequences:

>Translated_159_residues
MGEPMDDRTFRNAMGKFATGVTVVTTEFQGEVKGMTANAFMSVSLDPKLVVVSIGHKARMHDIVKQTGKFAVNLLTRDQE
DLSRLFAGQLKEERDVAFEWVDGHPILPDAMANVLCRVHSTYVAGDHTLYFGEVTHIVMKDEPGDPLLFFEGKYRSIGQ
>Mature_158_residues
GEPMDDRTFRNAMGKFATGVTVVTTEFQGEVKGMTANAFMSVSLDPKLVVVSIGHKARMHDIVKQTGKFAVNLLTRDQED
LSRLFAGQLKEERDVAFEWVDGHPILPDAMANVLCRVHSTYVAGDHTLYFGEVTHIVMKDEPGDPLLFFEGKYRSIGQ

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=157, Percent_Identity=29.9363057324841, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17721; Mature: 17589

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEPMDDRTFRNAMGKFATGVTVVTTEFQGEVKGMTANAFMSVSLDPKLVVVSIGHKARM
CCCCCCHHHHHHHHHHHHCCEEEEEEECCCEECCEEECEEEEEECCCEEEEEECCCHHHH
HDIVKQTGKFAVNLLTRDQEDLSRLFAGQLKEERDVAFEWVDGHPILPDAMANVLCRVHS
HHHHHHHHHEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
TYVAGDHTLYFGEVTHIVMKDEPGDPLLFFEGKYRSIGQ
EEECCCCEEEEEEEEEEEEECCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
GEPMDDRTFRNAMGKFATGVTVVTTEFQGEVKGMTANAFMSVSLDPKLVVVSIGHKARM
CCCCCHHHHHHHHHHHHCCEEEEEEECCCEECCEEECEEEEEECCCEEEEEECCCHHHH
HDIVKQTGKFAVNLLTRDQEDLSRLFAGQLKEERDVAFEWVDGHPILPDAMANVLCRVHS
HHHHHHHHHEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
TYVAGDHTLYFGEVTHIVMKDEPGDPLLFFEGKYRSIGQ
EEECCCCEEEEEEEEEEEEECCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]