Click here to switch to the map view.

The map label for this gene is pyk [H]

Identifier: 138896608

GI number: 138896608

Start: 3067178

End: 3069013

Strand: Reverse

Name: pyk [H]

Synonym: GTNG_2971

Alternate gene names: 138896608

Gene position: 3069013-3067178 (Counterclockwise)

Preceding gene: 138896610

Following gene: 138896607

Centisome position: 86.44

GC content: 49.78

Gene sequence:

>1836_bases
ATGCACGACAAACGGGATTTAGGTGCCCGATTAGCCGCCTTTTACCACGCGTTGGTCGAATGGGTCGCACAAAAACGAAA
ACATTTTCCACTTTCATCCCGTGAAGAAAGCCGGGATCAGTTGCTTGCTTATTTATATATGAACGAGCATATGCCGTTAG
ATTTAATGGAGGCACTTGAAGAAGAGGGCTTGCTGCTCGCTGGGCAAGAATCTCATCTCATCGCGGCCATGGAACAGTTT
TTGACTTATTTGCGCACGCCGTTTGATCGTAGGGAAGAGATCCGAAAGGTGAAAAGAAGAGAAGCGAAACGGCTGCTTGA
TGAACAGGTAGAAGCCATGTTTGGTCCACGGGGCCATTCCCGGCGGACACGCATCATGGTGACGATGGACGAAGCGTGGA
TCGATCATCCGGACATCGTGGAGCGGTTGCTTTTGTACGGCATGGACATTGCCCGTATTAATTGCGCCTACGGTTCGCCG
GAAACATGGGAGACGTTGGCCGCTGTCATCCGTCAGGCGGAAGAGCGTTTGCAACAACAACTGTTGGGAAAGCGCTGCCG
CATCTACATGGATTTACCGGGCCCGAAGATTCGGGTCGACCGTCTTGCCGTGAATACAGGACCGGTGAAGTTGGCAGTAC
AGAAAAATGAATATGGAGAGCCGGTTCAACCGTTGGTCGGTATCATTTCTTTCGACTCTTCCAACCTGTTTTCCCCGCTT
CCTCATGATGTTTCGTTCATCTTGGAGTTGACGGCTGAAGAGGAAGCAGTTGTGGAACAAGGGGACGAACTGTTTTTCAA
TGATGTGCGTGGGAAAAAACGAAAATTGAAGGTGATGGAACAGATAGGACCGTCTTGTTTTAAAGTTCTGCTTTCGCGAA
CGGCCTATGTGCAAAATGGGATGAAATTGTGGGGCGGGTCGGTATCGTTTACTCTTTCTTCCACGTTGTTCATTCCGATG
AAAGCACTTGTCAAAACTGGAACGACACTCTACATTTATTTCGATGAAGCCCAATTTGCGGCTGCAAAGGCCGAACAAGG
CGTAAAGATGACAACCACATTGGCGAAAGCATGGCGCAACGTGCGGGCGGGAGATCGGTTGTATTTAAACGATGGCAAAA
TTTTAGCCAGGGTCGTCAAGGTTCATGAATGGTGCATCGAGGCAAAAGTCATTGCCGATGGAGGAAAGCAGAAAACGATC
AAGCAAGGGACGGGCATTCATCTTCCTGACTCGTTCATCCAGTTGACCGTTCCGCGGCTTACCGACCGCGATCTTGAATT
GATTCCGTTCATCGCTAAGCATGCCGATATGGTCGGACTGTCATTTGTCCATACACCTTATGACTTGCGGAAGCTTTATC
GCTTGCTTTCAAAGCACGGGGCTTCTTCGCTCCCGGTGATTGCGAAAATTGAAACGAGGAAGGCGCTCCAAAACTTAGCC
CGCATTTTGCTTGAAGGGTTAAAGCTCCCGGCGTTCGGTGTGATGATCGCCCGCGGTGACTTAGCGCTTGAAGTCGGATT
TGAACATATGGCCGTCACCCAGCAAGAGATTTTGGCGCTATGCCGGGCGGCTCATGTTCCGGTCATTTGGGCGACGCAAG
TGCTTGAACAGCTAGCGAAAAAGGGGATGCCAGCGCGTGCGGAAATCTCGGATGTATCGTTAGGGAAGCAGGCGCAATGT
GTCATGTTGAATAAAGGAGCTCATATTGTTGAAGCTGTGCGGATGTTGGCTTCTTTATTGGAAAATGAAGAAGTGCAAAG
CCTACATCGGTGGGAAGCAGTGACACATGACAAGGAGCTGCCAAACTTATTCGCTTTGTTGAACGAAGAGGAGTAA

Upstream 100 bases:

>100_bases
AAAACCGGCGAATGACGTCGGTTTTTTTTCTGTCCTGATTTTTGGCGGCGGCGAATATATAGAAATAAAACCGCATTTTT
CACGAGGGTTGGTGCCACGA

Downstream 100 bases:

>100_bases
GAGGCGGGTACAGGCTTTCTCTTACTTGTATGTAAACTTATATAAATTAAAGATTAACAAAATGAGTGGTATCGTCAAAA
TAAAAACCAGCTGCAAAGGG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MHDKRDLGARLAAFYHALVEWVAQKRKHFPLSSREESRDQLLAYLYMNEHMPLDLMEALEEEGLLLAGQESHLIAAMEQF
LTYLRTPFDRREEIRKVKRREAKRLLDEQVEAMFGPRGHSRRTRIMVTMDEAWIDHPDIVERLLLYGMDIARINCAYGSP
ETWETLAAVIRQAEERLQQQLLGKRCRIYMDLPGPKIRVDRLAVNTGPVKLAVQKNEYGEPVQPLVGIISFDSSNLFSPL
PHDVSFILELTAEEEAVVEQGDELFFNDVRGKKRKLKVMEQIGPSCFKVLLSRTAYVQNGMKLWGGSVSFTLSSTLFIPM
KALVKTGTTLYIYFDEAQFAAAKAEQGVKMTTTLAKAWRNVRAGDRLYLNDGKILARVVKVHEWCIEAKVIADGGKQKTI
KQGTGIHLPDSFIQLTVPRLTDRDLELIPFIAKHADMVGLSFVHTPYDLRKLYRLLSKHGASSLPVIAKIETRKALQNLA
RILLEGLKLPAFGVMIARGDLALEVGFEHMAVTQQEILALCRAAHVPVIWATQVLEQLAKKGMPARAEISDVSLGKQAQC
VMLNKGAHIVEAVRMLASLLENEEVQSLHRWEAVTHDKELPNLFALLNEEE

Sequences:

>Translated_611_residues
MHDKRDLGARLAAFYHALVEWVAQKRKHFPLSSREESRDQLLAYLYMNEHMPLDLMEALEEEGLLLAGQESHLIAAMEQF
LTYLRTPFDRREEIRKVKRREAKRLLDEQVEAMFGPRGHSRRTRIMVTMDEAWIDHPDIVERLLLYGMDIARINCAYGSP
ETWETLAAVIRQAEERLQQQLLGKRCRIYMDLPGPKIRVDRLAVNTGPVKLAVQKNEYGEPVQPLVGIISFDSSNLFSPL
PHDVSFILELTAEEEAVVEQGDELFFNDVRGKKRKLKVMEQIGPSCFKVLLSRTAYVQNGMKLWGGSVSFTLSSTLFIPM
KALVKTGTTLYIYFDEAQFAAAKAEQGVKMTTTLAKAWRNVRAGDRLYLNDGKILARVVKVHEWCIEAKVIADGGKQKTI
KQGTGIHLPDSFIQLTVPRLTDRDLELIPFIAKHADMVGLSFVHTPYDLRKLYRLLSKHGASSLPVIAKIETRKALQNLA
RILLEGLKLPAFGVMIARGDLALEVGFEHMAVTQQEILALCRAAHVPVIWATQVLEQLAKKGMPARAEISDVSLGKQAQC
VMLNKGAHIVEAVRMLASLLENEEVQSLHRWEAVTHDKELPNLFALLNEEE
>Mature_611_residues
MHDKRDLGARLAAFYHALVEWVAQKRKHFPLSSREESRDQLLAYLYMNEHMPLDLMEALEEEGLLLAGQESHLIAAMEQF
LTYLRTPFDRREEIRKVKRREAKRLLDEQVEAMFGPRGHSRRTRIMVTMDEAWIDHPDIVERLLLYGMDIARINCAYGSP
ETWETLAAVIRQAEERLQQQLLGKRCRIYMDLPGPKIRVDRLAVNTGPVKLAVQKNEYGEPVQPLVGIISFDSSNLFSPL
PHDVSFILELTAEEEAVVEQGDELFFNDVRGKKRKLKVMEQIGPSCFKVLLSRTAYVQNGMKLWGGSVSFTLSSTLFIPM
KALVKTGTTLYIYFDEAQFAAAKAEQGVKMTTTLAKAWRNVRAGDRLYLNDGKILARVVKVHEWCIEAKVIADGGKQKTI
KQGTGIHLPDSFIQLTVPRLTDRDLELIPFIAKHADMVGLSFVHTPYDLRKLYRLLSKHGASSLPVIAKIETRKALQNLA
RILLEGLKLPAFGVMIARGDLALEVGFEHMAVTQQEILALCRAAHVPVIWATQVLEQLAKKGMPARAEISDVSLGKQAQC
VMLNKGAHIVEAVRMLASLLENEEVQSLHRWEAVTHDKELPNLFALLNEEE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=274, Percent_Identity=29.9270072992701, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI33286420, Length=274, Percent_Identity=29.9270072992701, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI33286418, Length=259, Percent_Identity=30.5019305019305, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI32967597, Length=239, Percent_Identity=32.6359832635983, Blast_Score=101, Evalue=3e-21,
Organism=Homo sapiens, GI10835121, Length=239, Percent_Identity=32.6359832635983, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310128732, Length=210, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI310128730, Length=210, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1787965, Length=333, Percent_Identity=25.8258258258258, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788160, Length=197, Percent_Identity=28.9340101522843, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17544584, Length=246, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17506829, Length=234, Percent_Identity=30.7692307692308, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71984406, Length=234, Percent_Identity=30.7692307692308, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71984413, Length=234, Percent_Identity=30.7692307692308, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17506831, Length=234, Percent_Identity=30.7692307692308, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324923, Length=255, Percent_Identity=29.8039215686275, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319279, Length=225, Percent_Identity=32.8888888888889, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571814, Length=237, Percent_Identity=32.0675105485232, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648964, Length=237, Percent_Identity=32.0675105485232, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648966, Length=227, Percent_Identity=32.5991189427313, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24581235, Length=207, Percent_Identity=28.0193236714976, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24646914, Length=186, Percent_Identity=28.494623655914, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 69155; Mature: 69155

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDKRDLGARLAAFYHALVEWVAQKRKHFPLSSREESRDQLLAYLYMNEHMPLDLMEALE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
EEGLLLAGQESHLIAAMEQFLTYLRTPFDRREEIRKVKRREAKRLLDEQVEAMFGPRGHS
HCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RRTRIMVTMDEAWIDHPDIVERLLLYGMDIARINCAYGSPETWETLAAVIRQAEERLQQQ
CCEEEEEEECHHHCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LLGKRCRIYMDLPGPKIRVDRLAVNTGPVKLAVQKNEYGEPVQPLVGIISFDSSNLFSPL
HHCCCCEEEEECCCCEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHEECCCCCCCCC
PHDVSFILELTAEEEAVVEQGDELFFNDVRGKKRKLKVMEQIGPSCFKVLLSRTAYVQNG
CCCHHHHEEECCCHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCC
MKLWGGSVSFTLSSTLFIPMKALVKTGTTLYIYFDEAQFAAAKAEQGVKMTTTLAKAWRN
HHHCCCCEEEEEHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHC
VRAGDRLYLNDGKILARVVKVHEWCIEAKVIADGGKQKTIKQGTGIHLPDSFIQLTVPRL
CCCCCEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHCCCCCCCCCHHHEEEECCC
TDRDLELIPFIAKHADMVGLSFVHTPYDLRKLYRLLSKHGASSLPVIAKIETRKALQNLA
CCCCCHHHHHHHHHCCHHCHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
RILLEGLKLPAFGVMIARGDLALEVGFEHMAVTQQEILALCRAAHVPVIWATQVLEQLAK
HHHHHHCCCCHHEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KGMPARAEISDVSLGKQAQCVMLNKGAHIVEAVRMLASLLENEEVQSLHRWEAVTHDKEL
CCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHC
PNLFALLNEEE
CHHHHHHCCCC
>Mature Secondary Structure
MHDKRDLGARLAAFYHALVEWVAQKRKHFPLSSREESRDQLLAYLYMNEHMPLDLMEALE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
EEGLLLAGQESHLIAAMEQFLTYLRTPFDRREEIRKVKRREAKRLLDEQVEAMFGPRGHS
HCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RRTRIMVTMDEAWIDHPDIVERLLLYGMDIARINCAYGSPETWETLAAVIRQAEERLQQQ
CCEEEEEEECHHHCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LLGKRCRIYMDLPGPKIRVDRLAVNTGPVKLAVQKNEYGEPVQPLVGIISFDSSNLFSPL
HHCCCCEEEEECCCCEEEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHEECCCCCCCCC
PHDVSFILELTAEEEAVVEQGDELFFNDVRGKKRKLKVMEQIGPSCFKVLLSRTAYVQNG
CCCHHHHEEECCCHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCC
MKLWGGSVSFTLSSTLFIPMKALVKTGTTLYIYFDEAQFAAAKAEQGVKMTTTLAKAWRN
HHHCCCCEEEEEHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHC
VRAGDRLYLNDGKILARVVKVHEWCIEAKVIADGGKQKTIKQGTGIHLPDSFIQLTVPRL
CCCCCEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCHHHCCCCCCCCCHHHEEEECCC
TDRDLELIPFIAKHADMVGLSFVHTPYDLRKLYRLLSKHGASSLPVIAKIETRKALQNLA
CCCCCHHHHHHHHHCCHHCHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
RILLEGLKLPAFGVMIARGDLALEVGFEHMAVTQQEILALCRAAHVPVIWATQVLEQLAK
HHHHHHCCCCHHEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KGMPARAEISDVSLGKQAQCVMLNKGAHIVEAVRMLASLLENEEVQSLHRWEAVTHDKEL
CCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHC
PNLFALLNEEE
CHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]