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The map label for this gene is pit [H]

Identifier: 138896534

GI number: 138896534

Start: 3003992

End: 3004990

Strand: Reverse

Name: pit [H]

Synonym: GTNG_2897

Alternate gene names: 138896534

Gene position: 3004990-3003992 (Counterclockwise)

Preceding gene: 138896535

Following gene: 138896532

Centisome position: 84.64

GC content: 50.45

Gene sequence:

>999_bases
ATGGATATGATCCTTGTCTTGACGGTATTAATTGTCGTTTTTGCGCTGGCGTTTGACTTTATTAACGGCTTCCATGACAC
AGCCAACGCCATCGCCACTTCTGTATCGACAAGAGCGCTCACGCCGCGCCGCGCGATCTTTTTAGCTGCTACGATGAACT
TCATTGGTGCCTTGACATTTACGGGCGTGGCCAAAACGATTACGAAAGACATCGTCGATCCATTTGCCTTAGAAAACGGA
CCGGTCATTATTTTAGCCGCCCTCACGTCAGCGATCGCTTGGAACTTGATTACTTGGTATTACGGCATTCCGAGCAGCTC
CTCGCACGCGCTGATCGGTTCGATTGCCGGAGCGGCGATTTCCGCGGCTGGGTTTGGGATTTTAAACTACAGCGGATTTT
TGAAAATTTTACAATCGCTCATCTTTTCGCCGTTCTTGGCGTTAGCAGTCGGCTTTGTCGTTATGACGATTTTTCGCATT
ATCTTTAAAAACGCGAACTTATACAGCACGACGAGAGGTTTTCGGCTGTTTCAAATCGTAACCGCCTCATTGCAGGCGTA
TACGCATGGGACGAACGACGCCCAAAAAGCGATGGGGATCATTACGATGGCATTGATCGCCGGCGGATATCACGCGACGA
CCGACATCCCTGAGTGGGTGCGCATTTCCGCTGCTACAGCGATGGGGCTTGGGACAGCCGTCGGTGGATGGAAAATCATT
AAAACGGTCGGCGGAAAGATCATGAAAATCCGCCCGATTAACGGGGCAGCAGCCGACTTATCATCCGCACTCATCATTTT
TTCAGCAACCACGTTCCATCTGCCGGTTAGTACGACGCACGTCATTTCCTCGGCGATTATGGGGGTTGGTGCCGCTCAGC
GGGTGAAAGGAGTCAAATGGGGCGTCGCCCGCCGGATTGTGCTCACATGGATCATCACATTGCCGATTTCGGCGTTAATT
GCTGCTTTTCTATACCAAGTGTTCAATTTGTTTTTCTAA

Upstream 100 bases:

>100_bases
TTTATGAAATGCTTGAAGAGATTGCCGATAGCTGTGAAGATGTCGCCAATACGCTTGAAACGATCATCATGCGCAACGCA
TAAAAGGGGACGTTGTTTTC

Downstream 100 bases:

>100_bases
CGAGAGATGCGCCTGCTATAAACAGGCGCGTTTTTTTATGCGGCGGGCCGGCTTGCGGAAACGGATCGGCCAATCGCCCG
ACAAAGACGTGGGGCGATCA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDMILVLTVLIVVFALAFDFINGFHDTANAIATSVSTRALTPRRAIFLAATMNFIGALTFTGVAKTITKDIVDPFALENG
PVIILAALTSAIAWNLITWYYGIPSSSSHALIGSIAGAAISAAGFGILNYSGFLKILQSLIFSPFLALAVGFVVMTIFRI
IFKNANLYSTTRGFRLFQIVTASLQAYTHGTNDAQKAMGIITMALIAGGYHATTDIPEWVRISAATAMGLGTAVGGWKII
KTVGGKIMKIRPINGAAADLSSALIIFSATTFHLPVSTTHVISSAIMGVGAAQRVKGVKWGVARRIVLTWIITLPISALI
AAFLYQVFNLFF

Sequences:

>Translated_332_residues
MDMILVLTVLIVVFALAFDFINGFHDTANAIATSVSTRALTPRRAIFLAATMNFIGALTFTGVAKTITKDIVDPFALENG
PVIILAALTSAIAWNLITWYYGIPSSSSHALIGSIAGAAISAAGFGILNYSGFLKILQSLIFSPFLALAVGFVVMTIFRI
IFKNANLYSTTRGFRLFQIVTASLQAYTHGTNDAQKAMGIITMALIAGGYHATTDIPEWVRISAATAMGLGTAVGGWKII
KTVGGKIMKIRPINGAAADLSSALIIFSATTFHLPVSTTHVISSAIMGVGAAQRVKGVKWGVARRIVLTWIITLPISALI
AAFLYQVFNLFF
>Mature_332_residues
MDMILVLTVLIVVFALAFDFINGFHDTANAIATSVSTRALTPRRAIFLAATMNFIGALTFTGVAKTITKDIVDPFALENG
PVIILAALTSAIAWNLITWYYGIPSSSSHALIGSIAGAAISAAGFGILNYSGFLKILQSLIFSPFLALAVGFVVMTIFRI
IFKNANLYSTTRGFRLFQIVTASLQAYTHGTNDAQKAMGIITMALIAGGYHATTDIPEWVRISAATAMGLGTAVGGWKII
KTVGGKIMKIRPINGAAADLSSALIIFSATTFHLPVSTTHVISSAIMGVGAAQRVKGVKWGVARRIVLTWIITLPISALI
AAFLYQVFNLFF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=159, Percent_Identity=31.4465408805031, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=31.5789473684211, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1789360, Length=217, Percent_Identity=32.258064516129, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=33.6405529953917, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17539280, Length=164, Percent_Identity=33.5365853658537, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25146401, Length=164, Percent_Identity=32.9268292682927, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=157, Percent_Identity=33.1210191082803, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566716, Length=173, Percent_Identity=28.3236994219653, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536725, Length=156, Percent_Identity=26.9230769230769, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=155, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356511, Length=183, Percent_Identity=26.775956284153, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35313; Mature: 35313

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMILVLTVLIVVFALAFDFINGFHDTANAIATSVSTRALTPRRAIFLAATMNFIGALTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVDPFALENGPVIILAALTSAIAWNLITWYYGIPSSSSHALIGSIAGAAI
HHHHHHHHHHHCCHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
SAAGFGILNYSGFLKILQSLIFSPFLALAVGFVVMTIFRIIFKNANLYSTTRGFRLFQIV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHH
TASLQAYTHGTNDAQKAMGIITMALIAGGYHATTDIPEWVRISAATAMGLGTAVGGWKII
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHCHHHHH
KTVGGKIMKIRPINGAAADLSSALIIFSATTFHLPVSTTHVISSAIMGVGAAQRVKGVKW
HHHCCCEEEEEECCCCHHHHHCEEEEEEEEEEEECCHHHHHHHHHHHCCCHHHHHCCCHH
GVARRIVLTWIITLPISALIAAFLYQVFNLFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDMILVLTVLIVVFALAFDFINGFHDTANAIATSVSTRALTPRRAIFLAATMNFIGALTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVDPFALENGPVIILAALTSAIAWNLITWYYGIPSSSSHALIGSIAGAAI
HHHHHHHHHHHCCHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
SAAGFGILNYSGFLKILQSLIFSPFLALAVGFVVMTIFRIIFKNANLYSTTRGFRLFQIV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHH
TASLQAYTHGTNDAQKAMGIITMALIAGGYHATTDIPEWVRISAATAMGLGTAVGGWKII
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHCHHHHH
KTVGGKIMKIRPINGAAADLSSALIIFSATTFHLPVSTTHVISSAIMGVGAAQRVKGVKW
HHHCCCEEEEEECCCCHHHHHCEEEEEEEEEEEECCHHHHHHHHHHHCCCHHHHHCCCHH
GVARRIVLTWIITLPISALIAAFLYQVFNLFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]