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The map label for this gene is leuA [H]

Identifier: 138896224

GI number: 138896224

Start: 2695746

End: 2697293

Strand: Reverse

Name: leuA [H]

Synonym: GTNG_2587

Alternate gene names: 138896224

Gene position: 2697293-2695746 (Counterclockwise)

Preceding gene: 138896225

Following gene: 138896223

Centisome position: 75.97

GC content: 50.71

Gene sequence:

>1548_bases
GTGCGAAAAATTAAGTTTTTTGATACGACCTTGCGCGACGGGGAACAATCGGCTGGTGTCAACTTGAACTTGCAAGAAAA
ATTGGAGATCGCCCGCCAGCTCGAGCGTCTGCAAGTCGACATTATCGAGGCGGGCTTCCCCGCCTCGTCCAAAGGCGATT
TTGAAGCAGTCAAACAAATCGCTGAGACGGTGAGAACATGTTCGGTCACCGGGCTGTCGCGCTCGGTCCGAAGTGATATT
GACGCAGCGTGGGAGGCATTAAAAGGCGGCGCTGAACCGCGGTTGCATCTGTTCATCGCCACTTCGCCGATCCATATGGT
GCACAAGTTGCGGATGACTCCGGAGCAAGTGATTGAAGCGGCGGTGGAAGCGGTGAAATATGCCAAACGCTTCTTCCCGA
TCGTTCAATGGTCGGCAGAAGATGCATGTCGGAGCGAGCTTCCGTTTTTAGCCAAAATCGTGACCGAAGTGATCAAGGCC
GGCGCTTCAGTGATCAATATCCCGGATACGGTCGGCTATATTACACCAAAAGAGTATGGGGAGATCTTCCTCTATTTACG
AAATAATGTTCCGAATATTGAAAACATTTCTTTGTCCGCCCATTGTCATGACGACTTAGGCATGGCGGTCATCAACTCAC
TGTCGGCCATCGAACATGGGGCGACGCAAGTTGAATGCACGATCAACGGCATCGGTGAGCGGGCCGGCAACGCGGCGCTT
GAGGAGATTGCCGTCGCCCTTCACATTCGCAAAGACTATTACCAAGTGGAAACGCGTCTCAATTTGCAAGAAATTAAGCG
CACGAGCAATCTAGTCAGCAAGCTGACCGGCATGGTCGTTCCGCCGAACAAAGCAGTTGTCGGCAAAAATGCTTTTGCTC
ACGAATCAGGCATCCATCAAGACGGCGTTTTAAAAGAAAAAACGACGTATGAAATCATCTCGCCAGAGCTCGTCGGTGTG
TCGTCGAATTCGATGGTACTTGGCAAACATTCCGGGCGCCATGCGCTCCGCAACCGGGTCGAAGAGCTCGGCTATACGCT
ATCAGAGGAAGAGATCAACCAGCTGTTCGTCCGCTTTAAAGAGTTGGCAGACAAAAAGAAAGATGTTACGGACGACGATT
TGATCGCTTTAATTTTCGAAGAGAAGTTCGACCATTTCAAAGACTTCTACCAATTGTCATCGATTCAAGTGCAATACGGG
ACAAATCAAATCCCGACAGCAGTTGTTGTACTGAAAGATGGGAAAGGAAACACCATTCAAGAAGCAGCGACTGGAGCTGG
CAGCGTCGAGGCGTTGTACAACACATTGGAGCGCTGTTTCCAAACGGCGGTGACGCTGTTAGATTACCGGATTGAATCGG
TCGGAGGCGGCCGTGATGCGCTAGCACAAGTGTTTGTCAAAGTGCGCGTCAACGATGTGGAAACGAGCGGCCGCGGAACG
GCGCAAGATGTGTTGGAAGCTTCAGCTAAAGCGTACATTAACGCGATGAACCGCATGTTTATGATCGAAGCGATGCGTGC
TGAAAACGAAAAAGTGACAACACCATAA

Upstream 100 bases:

>100_bases
AACCAGCGCGAAAATGAGCATTTGATCGAGGTCGTCGGACGCGAATTGCGGAGCATGATGCCGTTTGTGAAGGCAAAACA
AAAAGAGGTGGTGGTGCCAG

Downstream 100 bases:

>100_bases
AACGGGGGGATCGAGCATGGGAAACTATCGGATTGCCGTCTTGCCGGGAGATGGTATCGGCAAAGAAGTGACGTCTGGAG
CGGTGGAAGTATTAAAAGCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MRKIKFFDTTLRDGEQSAGVNLNLQEKLEIARQLERLQVDIIEAGFPASSKGDFEAVKQIAETVRTCSVTGLSRSVRSDI
DAAWEALKGGAEPRLHLFIATSPIHMVHKLRMTPEQVIEAAVEAVKYAKRFFPIVQWSAEDACRSELPFLAKIVTEVIKA
GASVINIPDTVGYITPKEYGEIFLYLRNNVPNIENISLSAHCHDDLGMAVINSLSAIEHGATQVECTINGIGERAGNAAL
EEIAVALHIRKDYYQVETRLNLQEIKRTSNLVSKLTGMVVPPNKAVVGKNAFAHESGIHQDGVLKEKTTYEIISPELVGV
SSNSMVLGKHSGRHALRNRVEELGYTLSEEEINQLFVRFKELADKKKDVTDDDLIALIFEEKFDHFKDFYQLSSIQVQYG
TNQIPTAVVVLKDGKGNTIQEAATGAGSVEALYNTLERCFQTAVTLLDYRIESVGGGRDALAQVFVKVRVNDVETSGRGT
AQDVLEASAKAYINAMNRMFMIEAMRAENEKVTTP

Sequences:

>Translated_515_residues
MRKIKFFDTTLRDGEQSAGVNLNLQEKLEIARQLERLQVDIIEAGFPASSKGDFEAVKQIAETVRTCSVTGLSRSVRSDI
DAAWEALKGGAEPRLHLFIATSPIHMVHKLRMTPEQVIEAAVEAVKYAKRFFPIVQWSAEDACRSELPFLAKIVTEVIKA
GASVINIPDTVGYITPKEYGEIFLYLRNNVPNIENISLSAHCHDDLGMAVINSLSAIEHGATQVECTINGIGERAGNAAL
EEIAVALHIRKDYYQVETRLNLQEIKRTSNLVSKLTGMVVPPNKAVVGKNAFAHESGIHQDGVLKEKTTYEIISPELVGV
SSNSMVLGKHSGRHALRNRVEELGYTLSEEEINQLFVRFKELADKKKDVTDDDLIALIFEEKFDHFKDFYQLSSIQVQYG
TNQIPTAVVVLKDGKGNTIQEAATGAGSVEALYNTLERCFQTAVTLLDYRIESVGGGRDALAQVFVKVRVNDVETSGRGT
AQDVLEASAKAYINAMNRMFMIEAMRAENEKVTTP
>Mature_515_residues
MRKIKFFDTTLRDGEQSAGVNLNLQEKLEIARQLERLQVDIIEAGFPASSKGDFEAVKQIAETVRTCSVTGLSRSVRSDI
DAAWEALKGGAEPRLHLFIATSPIHMVHKLRMTPEQVIEAAVEAVKYAKRFFPIVQWSAEDACRSELPFLAKIVTEVIKA
GASVINIPDTVGYITPKEYGEIFLYLRNNVPNIENISLSAHCHDDLGMAVINSLSAIEHGATQVECTINGIGERAGNAAL
EEIAVALHIRKDYYQVETRLNLQEIKRTSNLVSKLTGMVVPPNKAVVGKNAFAHESGIHQDGVLKEKTTYEIISPELVGV
SSNSMVLGKHSGRHALRNRVEELGYTLSEEEINQLFVRFKELADKKKDVTDDDLIALIFEEKFDHFKDFYQLSSIQVQYG
TNQIPTAVVVLKDGKGNTIQEAATGAGSVEALYNTLERCFQTAVTLLDYRIESVGGGRDALAQVFVKVRVNDVETSGRGT
AQDVLEASAKAYINAMNRMFMIEAMRAENEKVTTP

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=499, Percent_Identity=47.2945891783567, Blast_Score=444, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320071, Length=375, Percent_Identity=28.5333333333333, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320019, Length=375, Percent_Identity=28.5333333333333, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324225, Length=542, Percent_Identity=24.9077490774908, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324682, Length=543, Percent_Identity=24.3093922651934, Blast_Score=124, Evalue=5e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56917; Mature: 56917

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIKFFDTTLRDGEQSAGVNLNLQEKLEIARQLERLQVDIIEAGFPASSKGDFEAVKQI
CCCEEEECHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AETVRTCSVTGLSRSVRSDIDAAWEALKGGAEPRLHLFIATSPIHMVHKLRMTPEQVIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCHHHHHHH
AVEAVKYAKRFFPIVQWSAEDACRSELPFLAKIVTEVIKAGASVINIPDTVGYITPKEYG
HHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCC
EIFLYLRNNVPNIENISLSAHCHDDLGMAVINSLSAIEHGATQVECTINGIGERAGNAAL
CEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHH
EEIAVALHIRKDYYQVETRLNLQEIKRTSNLVSKLTGMVVPPNKAVVGKNAFAHESGIHQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEECCCCCEECCCCHHHCCCCCC
DGVLKEKTTYEIISPELVGVSSNSMVLGKHSGRHALRNRVEELGYTLSEEEINQLFVRFK
CCCCCCCCCCEEECCEEEECCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ELADKKKDVTDDDLIALIFEEKFDHFKDFYQLSSIQVQYGTNQIPTAVVVLKDGKGNTIQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCEEEEEEECCCCCCHH
EAATGAGSVEALYNTLERCFQTAVTLLDYRIESVGGGRDALAQVFVKVRVNDVETSGRGT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCCC
AQDVLEASAKAYINAMNRMFMIEAMRAENEKVTTP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRKIKFFDTTLRDGEQSAGVNLNLQEKLEIARQLERLQVDIIEAGFPASSKGDFEAVKQI
CCCEEEECHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AETVRTCSVTGLSRSVRSDIDAAWEALKGGAEPRLHLFIATSPIHMVHKLRMTPEQVIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCHHHHHHH
AVEAVKYAKRFFPIVQWSAEDACRSELPFLAKIVTEVIKAGASVINIPDTVGYITPKEYG
HHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCC
EIFLYLRNNVPNIENISLSAHCHDDLGMAVINSLSAIEHGATQVECTINGIGERAGNAAL
CEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHH
EEIAVALHIRKDYYQVETRLNLQEIKRTSNLVSKLTGMVVPPNKAVVGKNAFAHESGIHQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCEEECCCCCEECCCCHHHCCCCCC
DGVLKEKTTYEIISPELVGVSSNSMVLGKHSGRHALRNRVEELGYTLSEEEINQLFVRFK
CCCCCCCCCCEEECCEEEECCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ELADKKKDVTDDDLIALIFEEKFDHFKDFYQLSSIQVQYGTNQIPTAVVVLKDGKGNTIQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCEEEEEEECCCCCCHH
EAATGAGSVEALYNTLERCFQTAVTLLDYRIESVGGGRDALAQVFVKVRVNDVETSGRGT
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCCC
AQDVLEASAKAYINAMNRMFMIEAMRAENEKVTTP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA