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The map label for this gene is nifS [H]

Identifier: 138896169

GI number: 138896169

Start: 2637748

End: 2638764

Strand: Direct

Name: nifS [H]

Synonym: GTNG_2532

Alternate gene names: 138896169

Gene position: 2637748-2638764 (Clockwise)

Preceding gene: 138896153

Following gene: 138896170

Centisome position: 74.3

GC content: 54.57

Gene sequence:

>1017_bases
TTGCTGACGCTATGCCGGCGTGAGCTTGCCGCTATGATCAACGGGGAAGCAGAAGGGATTTATTTCACAAGCGGCGGCAC
GGAGGCGAATGTGCTTGCCATTCGGTCGCTCATTGCCGCCCACCGCCACAAAGGGAATCATTTAATCACTACCGAAGTCG
AACACGCCTCACTTTATCATTTGTTTCAACAGCTTGAAACGGAAGGATATGCGGTGACGTATTTGCCCGTCGATCGATTC
GGTCGCATCCGTCTCGCTGATTTGGAACGGGCCATCACACCGCAGACGATCCTCGCTTCCATCCAGCATGCCAACTCGGA
AATCGGCACCATCCAGCCGCTTGCTGACATCGGTCGCCTGTTGCGAGCGCGCGGCATCCTTTTTCATAGCGACTGCGTGC
AAACTTTTGCTAAAATACAGCTAGACGTTAAGGCGATGGCGATTGACAGCGTATCGATTTCCGCTCATAAAGTGTACGGT
CCAAAAGGCGTCGGAGCCGTCTACATCGACCCGCACCTTCATTGGAAACCCGTTTTTCCAGGGACGACACACGAATCCGG
CTTCCGACCCGGAACAGTCAATGTGCCGGGGATCGTTGCCTTCCTCACTGCCGCTAAGCAGCTTTACGAACGGATGGAAG
GTGAACAGACACGATTTGAACAGCTGCGCCGCCGGCTGCTTGACGCCATAGCAGCCAAACAGCTGCCGATTGCCGTTGAA
GGCCATCCTAGCTGTCGACTTGCCCATATTGTCGGGTTGTCTGTTACCGGTTTTGAAGGGCAGCTCGTCATGCTGGAATG
CAACCGTGCCGGTATTGCCATTTCAACTGGCAGCGCCTGCCAAGTCGGATTGCAGGCGCCGTCGCGCACCATGCTTGCCG
TCGGCAAAACCCCAGAGGAGGCAAAGCAGTTCGTCCGTGTATCACTTGGTACGTCCACGCGGGAAATAGAGATCGATCAA
TTGATCTCCACATTAGAACAGTTTATCCGAACGCGAAAGGAGGTATGGACAAGGTGA

Upstream 100 bases:

>100_bases
ACCAATGTGCAAAGAAGCCATCGAGGCTTATGCTGAGGCAGCGGCCGTTTATTTTGGCAATGAAAGTAGCCTGCACGACG
TCGGCACGAAAGCGAAGCAA

Downstream 100 bases:

>100_bases
AAGAAGAAAAGAAAATTTTAGGAGAAGCGCGGCGCCAGCTCATTTTGCAATGGCTGAAAGAAAGCGAGACACCGCTCACC
GGGGCAGAATTGGCAGCAAA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MLTLCRRELAAMINGEAEGIYFTSGGTEANVLAIRSLIAAHRHKGNHLITTEVEHASLYHLFQQLETEGYAVTYLPVDRF
GRIRLADLERAITPQTILASIQHANSEIGTIQPLADIGRLLRARGILFHSDCVQTFAKIQLDVKAMAIDSVSISAHKVYG
PKGVGAVYIDPHLHWKPVFPGTTHESGFRPGTVNVPGIVAFLTAAKQLYERMEGEQTRFEQLRRRLLDAIAAKQLPIAVE
GHPSCRLAHIVGLSVTGFEGQLVMLECNRAGIAISTGSACQVGLQAPSRTMLAVGKTPEEAKQFVRVSLGTSTREIEIDQ
LISTLEQFIRTRKEVWTR

Sequences:

>Translated_338_residues
MLTLCRRELAAMINGEAEGIYFTSGGTEANVLAIRSLIAAHRHKGNHLITTEVEHASLYHLFQQLETEGYAVTYLPVDRF
GRIRLADLERAITPQTILASIQHANSEIGTIQPLADIGRLLRARGILFHSDCVQTFAKIQLDVKAMAIDSVSISAHKVYG
PKGVGAVYIDPHLHWKPVFPGTTHESGFRPGTVNVPGIVAFLTAAKQLYERMEGEQTRFEQLRRRLLDAIAAKQLPIAVE
GHPSCRLAHIVGLSVTGFEGQLVMLECNRAGIAISTGSACQVGLQAPSRTMLAVGKTPEEAKQFVRVSLGTSTREIEIDQ
LISTLEQFIRTRKEVWTR
>Mature_338_residues
MLTLCRRELAAMINGEAEGIYFTSGGTEANVLAIRSLIAAHRHKGNHLITTEVEHASLYHLFQQLETEGYAVTYLPVDRF
GRIRLADLERAITPQTILASIQHANSEIGTIQPLADIGRLLRARGILFHSDCVQTFAKIQLDVKAMAIDSVSISAHKVYG
PKGVGAVYIDPHLHWKPVFPGTTHESGFRPGTVNVPGIVAFLTAAKQLYERMEGEQTRFEQLRRRLLDAIAAKQLPIAVE
GHPSCRLAHIVGLSVTGFEGQLVMLECNRAGIAISTGSACQVGLQAPSRTMLAVGKTPEEAKQFVRVSLGTSTREIEIDQ
LISTLEQFIRTRKEVWTR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Seems to be required for NAD biosynthesis [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=333, Percent_Identity=37.8378378378378, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI156713448, Length=369, Percent_Identity=30.8943089430894, Blast_Score=152, Evalue=3e-37,
Organism=Escherichia coli, GI48994898, Length=330, Percent_Identity=36.6666666666667, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1787970, Length=345, Percent_Identity=24.9275362318841, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=326, Percent_Identity=36.8098159509202, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI17533177, Length=343, Percent_Identity=30.9037900874636, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319831, Length=337, Percent_Identity=34.1246290801187, Blast_Score=210, Evalue=3e-55,
Organism=Drosophila melanogaster, GI20129463, Length=337, Percent_Identity=36.2017804154303, Blast_Score=204, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 37194; Mature: 37194

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLCRRELAAMINGEAEGIYFTSGGTEANVLAIRSLIAAHRHKGNHLITTEVEHASLYH
CCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHH
LFQQLETEGYAVTYLPVDRFGRIRLADLERAITPQTILASIQHANSEIGTIQPLADIGRL
HHHHHCCCCEEEEEEECCCCCCEEEEHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHH
LRARGILFHSDCVQTFAKIQLDVKAMAIDSVSISAHKVYGPKGVGAVYIDPHLHWKPVFP
HHHCCCEEEHHHHHHHHHHEEEEEEEEEECEEEEEEEEECCCCCCEEEECCCCEEEECCC
GTTHESGFRPGTVNVPGIVAFLTAAKQLYERMEGEQTRFEQLRRRLLDAIAAKQLPIAVE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEE
GHPSCRLAHIVGLSVTGFEGQLVMLECNRAGIAISTGSACQVGLQAPSRTMLAVGKTPEE
CCCCEEEEEEEEEEEECCCCEEEEEEECCCCEEEECCCCEEEECCCCCCEEEEECCCHHH
AKQFVRVSLGTSTREIEIDQLISTLEQFIRTRKEVWTR
HHHHEEHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTLCRRELAAMINGEAEGIYFTSGGTEANVLAIRSLIAAHRHKGNHLITTEVEHASLYH
CCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHH
LFQQLETEGYAVTYLPVDRFGRIRLADLERAITPQTILASIQHANSEIGTIQPLADIGRL
HHHHHCCCCEEEEEEECCCCCCEEEEHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHH
LRARGILFHSDCVQTFAKIQLDVKAMAIDSVSISAHKVYGPKGVGAVYIDPHLHWKPVFP
HHHCCCEEEHHHHHHHHHHEEEEEEEEEECEEEEEEEEECCCCCCEEEECCCCEEEECCC
GTTHESGFRPGTVNVPGIVAFLTAAKQLYERMEGEQTRFEQLRRRLLDAIAAKQLPIAVE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEE
GHPSCRLAHIVGLSVTGFEGQLVMLECNRAGIAISTGSACQVGLQAPSRTMLAVGKTPEE
CCCCEEEEEEEEEEEECCCCEEEEEEECCCCEEEECCCCEEEECCCCCCEEEEECCCHHH
AKQFVRVSLGTSTREIEIDQLISTLEQFIRTRKEVWTR
HHHHEEHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]