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The map label for this gene is mccA [H]

Identifier: 138896111

GI number: 138896111

Start: 2578390

End: 2579319

Strand: Reverse

Name: mccA [H]

Synonym: GTNG_2474

Alternate gene names: 138896111

Gene position: 2579319-2578390 (Counterclockwise)

Preceding gene: 138896112

Following gene: 138896110

Centisome position: 72.65

GC content: 52.58

Gene sequence:

>930_bases
ATGCAGGTAGCAAAAAGCATCCATGAGCTGATCGGTCATACACCAGTCATCGAAATTACGCGTTTTCCTTTGCCCGAAGG
GGTTCGTCTATTTGCAAAACTCGAGTACTTTAATCCAGGAGGAAGCATTAAAGACCGGCTCGGACAGGAGCTTTTGCGTG
AAGCGTTGGAATCGGGAAAGCTGAAAGAAGGCGGCACGATTATCGAACCGACCGCCGGCAATACTGGTATTGGTTTGGCG
TTAGCGGCGATCGGCAAAAATGTGAATGTCATTTTTTGCGTGCCGGAAAAATTCAGCATCGAAAAGCAGCAACTGATGAA
AGCGCTCGGAGCCCAAATCGTCCATACGCCGACTGAAGAGGGGATGGAAGGGGCGATCCGCAAAGCTGAAGAGCTTGCCC
GCACGATCCCGGGAGCGTATTGCCCGCAGCAGTTTCAAAATCCGGCCAATCCCAACACGTATTACAAGACACTTGGACCG
GAGTTGTGGGATGATCTGGACGGGCAAATCGATATTTTTGTCGCCGGAGCCGGTTCCGGCGGTACGTTTATGGGGACGGC
GACGTTTTTAAAAGAAAAAAATCCGGCCATTAAAACGGTCATTGTCGAACCAGAAGGCTCGATTTTAGGCGGCGGTGAGC
CCGGTCCACATCGGACGGAGGGAATTGGCATGGAGTTTTTGCCGCCGTTTATGGATCCGGATTATTTTGACGCCATCTAT
ACGATTCGCGATGATGACGCATTCCGCCGCGTGAGCGAACTGGCGCGGCAAGAAGGGCTTTTGGTCGGCAGCTCATCCGG
CGCCGCCTTTCATGCCGCCTTGCTTGAGGCTGAGAAGGCGAAGCCGGGGACAAACATCGTCGTCATTTTCCCTGACGGGA
GCGAGCGGTATTTAAGCAAAAACATTTACGAAGGTGGAGGATCCTCATGA

Upstream 100 bases:

>100_bases
GTCGATGTTGACGGTGGTCCAAAGCAAGGCTAAACAGTGAAAAGCGTAAAGGCGCCGCTGTCTCGCTGCCAAATGGACGT
GAAGAAACGGAGTGACTCAT

Downstream 100 bases:

>100_bases
GACGAAAAACGTTGCTCATTCATGGCGGCATTCCGGGTGACCCGCATACAGGGGCGGTGTCGGTGCCGATTTATCAAGTG
AGTACGTACAAGCAAGAAGA

Product: O-acetylserine lyase

Products: L-cysteine; acetate

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MQVAKSIHELIGHTPVIEITRFPLPEGVRLFAKLEYFNPGGSIKDRLGQELLREALESGKLKEGGTIIEPTAGNTGIGLA
LAAIGKNVNVIFCVPEKFSIEKQQLMKALGAQIVHTPTEEGMEGAIRKAEELARTIPGAYCPQQFQNPANPNTYYKTLGP
ELWDDLDGQIDIFVAGAGSGGTFMGTATFLKEKNPAIKTVIVEPEGSILGGGEPGPHRTEGIGMEFLPPFMDPDYFDAIY
TIRDDDAFRRVSELARQEGLLVGSSSGAAFHAALLEAEKAKPGTNIVVIFPDGSERYLSKNIYEGGGSS

Sequences:

>Translated_309_residues
MQVAKSIHELIGHTPVIEITRFPLPEGVRLFAKLEYFNPGGSIKDRLGQELLREALESGKLKEGGTIIEPTAGNTGIGLA
LAAIGKNVNVIFCVPEKFSIEKQQLMKALGAQIVHTPTEEGMEGAIRKAEELARTIPGAYCPQQFQNPANPNTYYKTLGP
ELWDDLDGQIDIFVAGAGSGGTFMGTATFLKEKNPAIKTVIVEPEGSILGGGEPGPHRTEGIGMEFLPPFMDPDYFDAIY
TIRDDDAFRRVSELARQEGLLVGSSSGAAFHAALLEAEKAKPGTNIVVIFPDGSERYLSKNIYEGGGSS
>Mature_309_residues
MQVAKSIHELIGHTPVIEITRFPLPEGVRLFAKLEYFNPGGSIKDRLGQELLREALESGKLKEGGTIIEPTAGNTGIGLA
LAAIGKNVNVIFCVPEKFSIEKQQLMKALGAQIVHTPTEEGMEGAIRKAEELARTIPGAYCPQQFQNPANPNTYYKTLGP
ELWDDLDGQIDIFVAGAGSGGTFMGTATFLKEKNPAIKTVIVEPEGSILGGGEPGPHRTEGIGMEFLPPFMDPDYFDAIY
TIRDDDAFRRVSELARQEGLLVGSSSGAAFHAALLEAEKAKPGTNIVVIFPDGSERYLSKNIYEGGGSS

Specific function: Catalyzes the conversion of O-acetylserine and homocysteine to cystathionine [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=310, Percent_Identity=39.6774193548387, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI295821200, Length=310, Percent_Identity=39.6774193548387, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI4557415, Length=310, Percent_Identity=39.6774193548387, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI1788754, Length=304, Percent_Identity=42.7631578947368, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI2367138, Length=298, Percent_Identity=36.5771812080537, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=40.2684563758389, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=38.3333333333333, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI115535073, Length=299, Percent_Identity=41.8060200668896, Blast_Score=207, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=38.9830508474576, Blast_Score=207, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17534315, Length=314, Percent_Identity=39.4904458598726, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17561720, Length=298, Percent_Identity=35.2348993288591, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25147552, Length=312, Percent_Identity=38.1410256410256, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=39.1509433962264, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321594, Length=323, Percent_Identity=40.5572755417957, Blast_Score=209, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6321449, Length=326, Percent_Identity=33.1288343558282, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24643623, Length=310, Percent_Identity=41.2903225806452, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI20129101, Length=310, Percent_Identity=41.2903225806452, Blast_Score=212, Evalue=2e-55,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47

Molecular weight: Translated: 33221; Mature: 33221

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVAKSIHELIGHTPVIEITRFPLPEGVRLFAKLEYFNPGGSIKDRLGQELLREALESGK
CCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
LKEGGTIIEPTAGNTGIGLALAAIGKNVNVIFCVPEKFSIEKQQLMKALGAQIVHTPTEE
CCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCEEECCCHH
GMEGAIRKAEELARTIPGAYCPQQFQNPANPNTYYKTLGPELWDDLDGQIDIFVAGAGSG
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHCCHHHHHCCCCEEEEEEEECCCC
GTFMGTATFLKEKNPAIKTVIVEPEGSILGGGEPGPHRTEGIGMEFLPPFMDPDYFDAIY
CCEEEHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCHHCCCCCCCCCHHCEEE
TIRDDDAFRRVSELARQEGLLVGSSSGAAFHAALLEAEKAKPGTNIVVIFPDGSERYLSK
EEECCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHH
NIYEGGGSS
HHCCCCCCC
>Mature Secondary Structure
MQVAKSIHELIGHTPVIEITRFPLPEGVRLFAKLEYFNPGGSIKDRLGQELLREALESGK
CCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
LKEGGTIIEPTAGNTGIGLALAAIGKNVNVIFCVPEKFSIEKQQLMKALGAQIVHTPTEE
CCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCCEEECCCHH
GMEGAIRKAEELARTIPGAYCPQQFQNPANPNTYYKTLGPELWDDLDGQIDIFVAGAGSG
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHCCHHHHHCCCCEEEEEEEECCCC
GTFMGTATFLKEKNPAIKTVIVEPEGSILGGGEPGPHRTEGIGMEFLPPFMDPDYFDAIY
CCEEEHHHHHHCCCCCEEEEEECCCCCEECCCCCCCCCCCCCCHHCCCCCCCCCHHCEEE
TIRDDDAFRRVSELARQEGLLVGSSSGAAFHAALLEAEKAKPGTNIVVIFPDGSERYLSK
EEECCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHH
NIYEGGGSS
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.7 {L-Ser}} 3.6 {S2-}} 0.1 {S2-}} 0.12 {acetyl-CoA}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} [C]

Substrates: O3-acetyl-L-serine; hydrogen sulfide

Specific reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]