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The map label for this gene is grpE

Identifier: 138896078

GI number: 138896078

Start: 2549141

End: 2549803

Strand: Reverse

Name: grpE

Synonym: GTNG_2441

Alternate gene names: 138896078

Gene position: 2549803-2549141 (Counterclockwise)

Preceding gene: 138896079

Following gene: 138896077

Centisome position: 71.82

GC content: 50.98

Gene sequence:

>663_bases
ATGGAACAAGGAGACAAACAAGCTACATACAATGAGCTGGAAACGGAACAGCCGGTTTCGAAAGAAGGGGCAGATTCGCA
ACCGGAAGACGAAGCCATTGGCGCCGCTTCGGAGCATGCAGATGCACAAGCCGGCGAGAATCCGGAGGCAGCTTCTACGA
CAGCTGATCCTGCCGAGCAAACGTCGGTTGAGGCGGAAGAACTGGCCAAAGCCAAGGCGCAAGTTGCTGAATTGGAAGAG
AAGTTAGCTGAGATGGAAAAGCGCTATCTTCGCCTGTACGCTGATTTTGAAAACTTCCGCCGCCGGGCACGTCAAGAGAT
GGAAGCGGCTGAAAAATACCGCGCCCAAAGTTTGGCGAGCGATTTGCTTCCTGTGCTCGACAACTTTGAGCGTGCGTTGA
AAATAGAGACGGAAAACGAACAAGCAAAATCGATTTTGCAAGGGGTAGAAATGGTGTACCGTTCGCTCCTTGACGCGTTA
AGAAAAGAAGGTGTCGAGGTGATTGAAGCGGTTGGCAAACCGTTTGATCCCCATTTACACCAAGCAGTTATGCAGACGGA
TGAAGGCGGCTATGAGCCGAATACGGTCGTGGAGGAGCTGCAAAAAGGCTATAAGTTAAAAGACCGCATTCTCCGTCCCG
CTATGGTAAAAGTGAGCCAATAA

Upstream 100 bases:

>100_bases
AGTAAGGATGGCCATATTGGTTAACGGATGGATGCGCGTCCATCCTTTCCTTGTGGCCATTGATACATAAATCGCAAACC
CTGATAGGGAGGTGAAGGCA

Downstream 100 bases:

>100_bases
TGGAAACGGAGGGCGATGAGCGATGAGCAAAATTATCGGCATTGACTTAGGAACGACAAACTCTTGCGTCGCTGTGTTGG
AAGGCGGCGAAGCGAAAGTC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MEQGDKQATYNELETEQPVSKEGADSQPEDEAIGAASEHADAQAGENPEAASTTADPAEQTSVEAEELAKAKAQVAELEE
KLAEMEKRYLRLYADFENFRRRARQEMEAAEKYRAQSLASDLLPVLDNFERALKIETENEQAKSILQGVEMVYRSLLDAL
RKEGVEVIEAVGKPFDPHLHQAVMQTDEGGYEPNTVVEELQKGYKLKDRILRPAMVKVSQ

Sequences:

>Translated_220_residues
MEQGDKQATYNELETEQPVSKEGADSQPEDEAIGAASEHADAQAGENPEAASTTADPAEQTSVEAEELAKAKAQVAELEE
KLAEMEKRYLRLYADFENFRRRARQEMEAAEKYRAQSLASDLLPVLDNFERALKIETENEQAKSILQGVEMVYRSLLDAL
RKEGVEVIEAVGKPFDPHLHQAVMQTDEGGYEPNTVVEELQKGYKLKDRILRPAMVKVSQ
>Mature_220_residues
MEQGDKQATYNELETEQPVSKEGADSQPEDEAIGAASEHADAQAGENPEAASTTADPAEQTSVEAEELAKAKAQVAELEE
KLAEMEKRYLRLYADFENFRRRARQEMEAAEKYRAQSLASDLLPVLDNFERALKIETENEQAKSILQGVEMVYRSLLDAL
RKEGVEVIEAVGKPFDPHLHQAVMQTDEGGYEPNTVVEELQKGYKLKDRILRPAMVKVSQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=149, Percent_Identity=36.9127516778523, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI40255109, Length=156, Percent_Identity=32.0512820512821, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1788967, Length=188, Percent_Identity=35.6382978723404, Blast_Score=118, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17552458, Length=150, Percent_Identity=31.3333333333333, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=160, Percent_Identity=33.75, Blast_Score=99, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24653432, Length=178, Percent_Identity=35.9550561797753, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_GEOTN (A4IR32)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001126531.1
- ProteinModelPortal:   A4IR32
- SMR:   A4IR32
- STRING:   A4IR32
- GeneID:   4967367
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_2441
- NMPDR:   fig|420246.5.peg.2347
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- PhylomeDB:   A4IR32
- ProtClustDB:   PRK14140
- BioCyc:   GTHE420246:GTNG_2441-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 24612; Mature: 24612

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQGDKQATYNELETEQPVSKEGADSQPEDEAIGAASEHADAQAGENPEAASTTADPAEQ
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCCCCCCCCHHH
TSVEAEELAKAKAQVAELEEKLAEMEKRYLRLYADFENFRRRARQEMEAAEKYRAQSLAS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLLPVLDNFERALKIETENEQAKSILQGVEMVYRSLLDALRKEGVEVIEAVGKPFDPHLH
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QAVMQTDEGGYEPNTVVEELQKGYKLKDRILRPAMVKVSQ
HHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEQGDKQATYNELETEQPVSKEGADSQPEDEAIGAASEHADAQAGENPEAASTTADPAEQ
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCCCCCCCCCCCCCHHH
TSVEAEELAKAKAQVAELEEKLAEMEKRYLRLYADFENFRRRARQEMEAAEKYRAQSLAS
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLLPVLDNFERALKIETENEQAKSILQGVEMVYRSLLDALRKEGVEVIEAVGKPFDPHLH
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
QAVMQTDEGGYEPNTVVEELQKGYKLKDRILRPAMVKVSQ
HHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA