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The map label for this gene is tnpA [H]

Identifier: 138895716

GI number: 138895716

Start: 2201942

End: 2203081

Strand: Direct

Name: tnpA [H]

Synonym: GTNG_2072

Alternate gene names: 138895716

Gene position: 2201942-2203081 (Clockwise)

Preceding gene: 138895714

Following gene: 138895717

Centisome position: 62.02

GC content: 37.11

Gene sequence:

>1140_bases
ATGAGGGTGCAAGAAGTTCTCTTAGAGAATAACATCAGGAGATATATATTAGTGGATGAAGAAGGATTTCCTGTGATTCC
TGTGGTGAAGTATCTGAAGTATTTAGATACGACAGGGAAAAGCAGAAATACACTGAAGACATACTGTTATGCGTTAAAGC
AGTATTTTGTGTTTTTACAGGAAAAAAAGAAGGATTACAGGGAAGTTCGTTTAGAGGACTTGGTGGAATTTGTAGTTTGG
TTGAGGAATCCATATGAAAGTGCAAAGGTAACTTCCCTCCAACCTGTGAAAGCAAAGAAAACAGAAAAGACGATTAATTT
GACGATTACAGCCGTCACCAATTTTTATGATTATTTGTATCGGAACGAGGAAATCCAAAACGATATGGTTGATAAGCTAC
TGAAACAAGTGTTTACAGGAGGAAGAACCAGATACAAGGATTTTCTGTATCATGTCAATAAAAATAAGCCTTCTAACCGT
CATATTCTGAAGGTAAAAGAGCCGAGAAAAAAGCTGAAAGTTTTAACGAAAGAAGAAGTACAACAATTGATAGAAGCAAC
CACCAACATCCGAGATAGACTGTTAATTCAATTGCTTTTTGAAACAGGATTGCGGATTGGTGAAGCACTTTCCTTGTTTA
TAGAGGATTTTATTTTTGACCATAAAAATGGACATCGGATTCGTTTAGTTAATCGAGGGGAACTGGAAAACGGCGCAAAA
CTAAAAACAGGAGAACGGGAAATTTATGTTTCCCAAGCCTTAATGGATTTGTTGGATGATTACTTGTATGAGGTAGTGGA
TGAATTAAACTTAAATACGAACTTTGTGTTTGTCAAACTTCGAGGCAAGAATGTAGGACAACCTATGACCTATTGCGATG
TAGAAGCCTTATTTAAGCGATTGAGAAAGAAAACAGGAATTCATGTTCACCCTCACCTTTTACGCCATACACATGCCACC
ATGTACTACCAACAAACGAAAGACATTAAACAAGTACAAGAACGATTAGGGCATTCCCAAATTCAAACCACAATGAACTT
GTACCTCCATCCTTCGGATGAAGAAATTCGTGAGAATTGGGAAAAGGCTCAACATGCGTTTCATATCACAAAAAAATCTG
GAAAGGATTCTGGAGAATGA

Upstream 100 bases:

>100_bases
ATGTTTTTATTATGAGGAGGAGGACGTAGCCCACAGCGGGAGGCGTAGCGAGGTCTTTAGACCGAGCGTAAAAGAGAAAG
ACAATAGGTGGTGAAGGACT

Downstream 100 bases:

>100_bases
GTATTCAACAGCACCATATCCATGATGAATTAGGCTTTCATGAACGATTACAGCAAGAGTTATCGACCTATACAGAAAAA
ATCATTCACGAAGATGGCTC

Product: transposition regulatory protein TnpA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRVQEVLLENNIRRYILVDEEGFPVIPVVKYLKYLDTTGKSRNTLKTYCYALKQYFVFLQEKKKDYREVRLEDLVEFVVW
LRNPYESAKVTSLQPVKAKKTEKTINLTITAVTNFYDYLYRNEEIQNDMVDKLLKQVFTGGRTRYKDFLYHVNKNKPSNR
HILKVKEPRKKLKVLTKEEVQQLIEATTNIRDRLLIQLLFETGLRIGEALSLFIEDFIFDHKNGHRIRLVNRGELENGAK
LKTGEREIYVSQALMDLLDDYLYEVVDELNLNTNFVFVKLRGKNVGQPMTYCDVEALFKRLRKKTGIHVHPHLLRHTHAT
MYYQQTKDIKQVQERLGHSQIQTTMNLYLHPSDEEIRENWEKAQHAFHITKKSGKDSGE

Sequences:

>Translated_379_residues
MRVQEVLLENNIRRYILVDEEGFPVIPVVKYLKYLDTTGKSRNTLKTYCYALKQYFVFLQEKKKDYREVRLEDLVEFVVW
LRNPYESAKVTSLQPVKAKKTEKTINLTITAVTNFYDYLYRNEEIQNDMVDKLLKQVFTGGRTRYKDFLYHVNKNKPSNR
HILKVKEPRKKLKVLTKEEVQQLIEATTNIRDRLLIQLLFETGLRIGEALSLFIEDFIFDHKNGHRIRLVNRGELENGAK
LKTGEREIYVSQALMDLLDDYLYEVVDELNLNTNFVFVKLRGKNVGQPMTYCDVEALFKRLRKKTGIHVHPHLLRHTHAT
MYYQQTKDIKQVQERLGHSQIQTTMNLYLHPSDEEIRENWEKAQHAFHITKKSGKDSGE
>Mature_379_residues
MRVQEVLLENNIRRYILVDEEGFPVIPVVKYLKYLDTTGKSRNTLKTYCYALKQYFVFLQEKKKDYREVRLEDLVEFVVW
LRNPYESAKVTSLQPVKAKKTEKTINLTITAVTNFYDYLYRNEEIQNDMVDKLLKQVFTGGRTRYKDFLYHVNKNKPSNR
HILKVKEPRKKLKVLTKEEVQQLIEATTNIRDRLLIQLLFETGLRIGEALSLFIEDFIFDHKNGHRIRLVNRGELENGAK
LKTGEREIYVSQALMDLLDDYLYEVVDELNLNTNFVFVKLRGKNVGQPMTYCDVEALFKRLRKKTGIHVHPHLLRHTHAT
MYYQQTKDIKQVQERLGHSQIQTTMNLYLHPSDEEIRENWEKAQHAFHITKKSGKDSGE

Specific function: One of three proteins encoded by transposon Tn554 required for its transposition [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1790244, Length=253, Percent_Identity=24.1106719367589, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109
- InterPro:   IPR004107 [H]

Pfam domain/function: PF02899 Phage_integr_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 44903; Mature: 44903

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVQEVLLENNIRRYILVDEEGFPVIPVVKYLKYLDTTGKSRNTLKTYCYALKQYFVFLQ
CCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EKKKDYREVRLEDLVEFVVWLRNPYESAKVTSLQPVKAKKTEKTINLTITAVTNFYDYLY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHH
RNEEIQNDMVDKLLKQVFTGGRTRYKDFLYHVNKNKPSNRHILKVKEPRKKLKVLTKEEV
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHH
QQLIEATTNIRDRLLIQLLFETGLRIGEALSLFIEDFIFDHKNGHRIRLVNRGELENGAK
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
LKTGEREIYVSQALMDLLDDYLYEVVDELNLNTNFVFVKLRGKNVGQPMTYCDVEALFKR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
LRKKTGIHVHPHLLRHTHATMYYQQTKDIKQVQERLGHSQIQTTMNLYLHPSDEEIRENW
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEECCCHHHHHHHH
EKAQHAFHITKKSGKDSGE
HHHHHHEEEECCCCCCCCC
>Mature Secondary Structure
MRVQEVLLENNIRRYILVDEEGFPVIPVVKYLKYLDTTGKSRNTLKTYCYALKQYFVFLQ
CCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EKKKDYREVRLEDLVEFVVWLRNPYESAKVTSLQPVKAKKTEKTINLTITAVTNFYDYLY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHH
RNEEIQNDMVDKLLKQVFTGGRTRYKDFLYHVNKNKPSNRHILKVKEPRKKLKVLTKEEV
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHH
QQLIEATTNIRDRLLIQLLFETGLRIGEALSLFIEDFIFDHKNGHRIRLVNRGELENGAK
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
LKTGEREIYVSQALMDLLDDYLYEVVDELNLNTNFVFVKLRGKNVGQPMTYCDVEALFKR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
LRKKTGIHVHPHLLRHTHATMYYQQTKDIKQVQERLGHSQIQTTMNLYLHPSDEEIRENW
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEECCCHHHHHHHH
EKAQHAFHITKKSGKDSGE
HHHHHHEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]