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The map label for this gene is cbiF [H]

Identifier: 138895343

GI number: 138895343

Start: 1777674

End: 1778447

Strand: Reverse

Name: cbiF [H]

Synonym: GTNG_1687

Alternate gene names: 138895343

Gene position: 1778447-1777674 (Counterclockwise)

Preceding gene: 138895344

Following gene: 138895342

Centisome position: 50.09

GC content: 58.79

Gene sequence:

>774_bases
GTGAAGCTGTATATCATCGGAGCGGGGCCGGGAGCGCCGGATTTGATCACCGTTCGAGGAGCGGAGCTGTTGGCCGCGGC
GGACGCCATTTTTTACACCGACTCGTTAGTGAGCGAAGAACTGATTGAGCGTTACCGGAAGCCGGAGGCAGAGGTGTTTC
ATACAGCCGGCATGCATTTGGAACAAATGGTGGCGATGATGGCCGACAAGGTGAGGCAAGGCCGGCTCGTCGTCCGCGTG
CATACGGGTGATCCATCGATTTACGGGGCGACGCTCGAGCAAATCGCCTTATTGAAAGGAGAAGGAATTGAAGTCGAAAT
CGTCCCCGGTGTCAGTTCGGCGTTTGCGGCCGCGGCCGCGGTGCAGGCGGAGCTCACCGTCCCCGAATTGACGCAGACGG
TCATTTTCACCCGCGCCGAGGGGCGGACGCCGGTGCCGCCGCGTGAGAAATTGCAGGAGCTCGCCCAGCATCATTGCACC
CTTGTCCTCTTTTTAAGCGCGACGCTTGCCAAAAAAGTAAGCGCGGCGCTTCTTGAAGCGGGTTGGAGCGGTGATACGCC
TGTCGCGATCGTCTATAAGGCGACATGGCCGGATGAAGTCGTGATTCGTTCCACCGTTGCCACAATGGCTGACGATATGC
GGCGCCATGGTGTGACGAAACACGCGATCATGTTGGCCGGTTGGGCGCTCGATCCCCATATTCATGAGCGCGGCTACCGT
TCGAAGCTGTACGACCGCACGTTTACGCACGGATATCGAAAGGGGGAGAAGTAA

Upstream 100 bases:

>100_bases
CAGTGGTGACGAAAGTGACGTCAAGGGAAGAAGTCATTTGGGACGTTGAGCGGCTCGAAGGGGCAGAACTCGAATATTTG
ACATTGCTGGTGGTGAGAAA

Downstream 100 bases:

>100_bases
GGTGGGAGTTGCTGCCGCGAACAACAAAAGACACGGCGCCTATGCTGTCGTGGCCATTACGAAACACGGGGTCGAGATCG
CCCGACGCCTTGGAGGCGCT

Product: precorrin-4 methylase

Products: 2S-adenosyl-L-homocysteine; precorrin; sirohydrochlorin; NADH; Proton; siroheme [C]

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKLYIIGAGPGAPDLITVRGAELLAAADAIFYTDSLVSEELIERYRKPEAEVFHTAGMHLEQMVAMMADKVRQGRLVVRV
HTGDPSIYGATLEQIALLKGEGIEVEIVPGVSSAFAAAAAVQAELTVPELTQTVIFTRAEGRTPVPPREKLQELAQHHCT
LVLFLSATLAKKVSAALLEAGWSGDTPVAIVYKATWPDEVVIRSTVATMADDMRRHGVTKHAIMLAGWALDPHIHERGYR
SKLYDRTFTHGYRKGEK

Sequences:

>Translated_257_residues
MKLYIIGAGPGAPDLITVRGAELLAAADAIFYTDSLVSEELIERYRKPEAEVFHTAGMHLEQMVAMMADKVRQGRLVVRV
HTGDPSIYGATLEQIALLKGEGIEVEIVPGVSSAFAAAAAVQAELTVPELTQTVIFTRAEGRTPVPPREKLQELAQHHCT
LVLFLSATLAKKVSAALLEAGWSGDTPVAIVYKATWPDEVVIRSTVATMADDMRRHGVTKHAIMLAGWALDPHIHERGYR
SKLYDRTFTHGYRKGEK
>Mature_257_residues
MKLYIIGAGPGAPDLITVRGAELLAAADAIFYTDSLVSEELIERYRKPEAEVFHTAGMHLEQMVAMMADKVRQGRLVVRV
HTGDPSIYGATLEQIALLKGEGIEVEIVPGVSSAFAAAAAVQAELTVPELTQTVIFTRAEGRTPVPPREKLQELAQHHCT
LVLFLSATLAKKVSAALLEAGWSGDTPVAIVYKATWPDEVVIRSTVATMADDMRRHGVTKHAIMLAGWALDPHIHERGYR
SKLYDRTFTHGYRKGEK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=215, Percent_Identity=29.7674418604651, Blast_Score=94, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6322922, Length=239, Percent_Identity=25.9414225941423, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.107; 1.3.1.76; 4.99.1.4 [C]

Molecular weight: Translated: 28110; Mature: 28110

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYIIGAGPGAPDLITVRGAELLAAADAIFYTDSLVSEELIERYRKPEAEVFHTAGMHL
CEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCH
EQMVAMMADKVRQGRLVVRVHTGDPSIYGATLEQIALLKGEGIEVEIVPGVSSAFAAAAA
HHHHHHHHHHHHCCEEEEEEECCCCCEEHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
VQAELTVPELTQTVIFTRAEGRTPVPPREKLQELAQHHCTLVLFLSATLAKKVSAALLEA
HHHCCCCHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWSGDTPVAIVYKATWPDEVVIRSTVATMADDMRRHGVTKHAIMLAGWALDPHIHERGYR
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCCHHHHCCHH
SKLYDRTFTHGYRKGEK
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKLYIIGAGPGAPDLITVRGAELLAAADAIFYTDSLVSEELIERYRKPEAEVFHTAGMHL
CEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCH
EQMVAMMADKVRQGRLVVRVHTGDPSIYGATLEQIALLKGEGIEVEIVPGVSSAFAAAAA
HHHHHHHHHHHHCCEEEEEEECCCCCEEHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
VQAELTVPELTQTVIFTRAEGRTPVPPREKLQELAQHHCTLVLFLSATLAKKVSAALLEA
HHHCCCCHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWSGDTPVAIVYKATWPDEVVIRSTVATMADDMRRHGVTKHAIMLAGWALDPHIHERGYR
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCCCCHHHHCCHH
SKLYDRTFTHGYRKGEK
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2S-Adenosyl-L-methionine; uroporphyrin III; dihydrosirohydrochlorin; NAD; sirohydrochlorin; Fe (II) [C]

Specific reaction: 2S-Adenosyl-L-methionine + uroporphyrin III = 2S-adenosyl-L-homocysteine + precorrin dihydrosirohydrochlorin + NAD = sirohydrochlorin + NADH sirohydrochlorin + Fe (II) = 2 Proton + siroheme [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9742225; 9660189 [H]