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The map label for this gene is thiM

Identifier: 138895022

GI number: 138895022

Start: 1448602

End: 1449411

Strand: Direct

Name: thiM

Synonym: GTNG_1360

Alternate gene names: 138895022

Gene position: 1448602-1449411 (Clockwise)

Preceding gene: 138895021

Following gene: 138895023

Centisome position: 40.8

GC content: 58.15

Gene sequence:

>810_bases
ATGGGCAACTGGCACGAATGGGCGGAACTGCTCGAGCGGGTGCGAGGGACGAAACCGCTCGTCCATAATATTACGAATGT
CGTCGTGACGAATTTTACGGCGAACGGGCTGTTGGCGCTCGGCGCTTCGCCGGTGATGGCGTACGCGAAAGAGGAAGTGG
CCGAGATGGCGAAGCTTGCAGGAGCGCTTGTGTTAAATATTGGAACGTTGAATGCAACGGAAGTCGAGGCGATGCTTATT
GCCGGCCGGGCGGCTAATGAGGCCGGGGTACCGGTGATTTTCGATCCGGTCGGTGCCGGGGCGACCAGCTACCGAACAGA
GACGGCGCACCGCATCGCCGAACAGATTCAACTCGCGGTTGTGCGCGGCAATGCGGCGGAAATCGCCAATATGATCGGTG
AATCGTGGATGATTAAAGGAGTCGACGCCGGTGAAGGAAGCGGGGATGTGGTCGCTTTGGCCAAACGGGCGGCAGCCAAG
CTCGGCACCGTCGTGGCAATTACCGGAAAAGAGGATGTCGTCACAGATGGACAGGCAGCATATTTGATTCACAACGGCCA
TCCGCTGTTGACAAAAGTGACCGGGGCAGGTTGTTTGTTAACGTCCGTCGTTGGAGCGTTTGCGGCTGTTGAGCAGGATG
CTGTGAAGGCAGCGGTTGCGGCGTTAACCTATTACGGAGTCGCAGCTGAACAGGCAGCCGCCGAAGCCGGACAACGCGGC
CCAGGCAGTTTTCAGGTGGCGTTTTTGGACGCCTTGGCTCGCATTGGCGTTGATGATGTGAAGCGTGAGGGACGGGTTGA
ACAACGGTAA

Upstream 100 bases:

>100_bases
GATGAATGGGACAAATTCATCATTCTGTAAGACGGAAGTATGGCGGTCGTACCTAACACCGCCAAGGCAGAGGCATCGAT
AGGAAAGGGGAGAATCGGAG

Downstream 100 bases:

>100_bases
AAGGGGAGGAGAACCATGACGGTGTATAAAGCGTTGACGATTGCTGGGTCGGACAGCGGCGGTGGCGCCGGCATTCAAGC
GGATTTAAAAACATTTCAAG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MGNWHEWAELLERVRGTKPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMAKLAGALVLNIGTLNATEVEAMLI
AGRAANEAGVPVIFDPVGAGATSYRTETAHRIAEQIQLAVVRGNAAEIANMIGESWMIKGVDAGEGSGDVVALAKRAAAK
LGTVVAITGKEDVVTDGQAAYLIHNGHPLLTKVTGAGCLLTSVVGAFAAVEQDAVKAAVAALTYYGVAAEQAAAEAGQRG
PGSFQVAFLDALARIGVDDVKREGRVEQR

Sequences:

>Translated_269_residues
MGNWHEWAELLERVRGTKPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMAKLAGALVLNIGTLNATEVEAMLI
AGRAANEAGVPVIFDPVGAGATSYRTETAHRIAEQIQLAVVRGNAAEIANMIGESWMIKGVDAGEGSGDVVALAKRAAAK
LGTVVAITGKEDVVTDGQAAYLIHNGHPLLTKVTGAGCLLTSVVGAFAAVEQDAVKAAVAALTYYGVAAEQAAAEAGQRG
PGSFQVAFLDALARIGVDDVKREGRVEQR
>Mature_268_residues
GNWHEWAELLERVRGTKPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMAKLAGALVLNIGTLNATEVEAMLIA
GRAANEAGVPVIFDPVGAGATSYRTETAHRIAEQIQLAVVRGNAAEIANMIGESWMIKGVDAGEGSGDVVALAKRAAAKL
GTVVAITGKEDVVTDGQAAYLIHNGHPLLTKVTGAGCLLTSVVGAFAAVEQDAVKAAVAALTYYGVAAEQAAAEAGQRGP
GSFQVAFLDALARIGVDDVKREGRVEQR

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=248, Percent_Identity=40.3225806451613, Blast_Score=164, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6325042, Length=274, Percent_Identity=31.7518248175182, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_GEOTN (A4IN26)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001125475.1
- ProteinModelPortal:   A4IN26
- SMR:   A4IN26
- STRING:   A4IN26
- GeneID:   4966299
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_1360
- NMPDR:   fig|420246.5.peg.1319
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- PhylomeDB:   A4IN26
- ProtClustDB:   PRK09355
- BioCyc:   GTHE420246:GTNG_1360-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27781; Mature: 27650

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: BINDING 46-46 BINDING 122-122 BINDING 168-168 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNWHEWAELLERVRGTKPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMAKLA
CCCHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHH
GALVLNIGTLNATEVEAMLIAGRAANEAGVPVIFDPVGAGATSYRTETAHRIAEQIQLAV
HHHEEEECCCCHHHHHHHEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHEEEE
VRGNAAEIANMIGESWMIKGVDAGEGSGDVVALAKRAAAKLGTVVAITGKEDVVTDGQAA
EECCHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCEEECCCEE
YLIHNGHPLLTKVTGAGCLLTSVVGAFAAVEQDAVKAAVAALTYYGVAAEQAAAEAGQRG
EEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PGSFQVAFLDALARIGVDDVKREGRVEQR
CCCEEHHHHHHHHHCCHHHHHHCCCCCCC
>Mature Secondary Structure 
GNWHEWAELLERVRGTKPLVHNITNVVVTNFTANGLLALGASPVMAYAKEEVAEMAKLA
CCHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHH
GALVLNIGTLNATEVEAMLIAGRAANEAGVPVIFDPVGAGATSYRTETAHRIAEQIQLAV
HHHEEEECCCCHHHHHHHEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHEEEE
VRGNAAEIANMIGESWMIKGVDAGEGSGDVVALAKRAAAKLGTVVAITGKEDVVTDGQAA
EECCHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCEEECCCEE
YLIHNGHPLLTKVTGAGCLLTSVVGAFAAVEQDAVKAAVAALTYYGVAAEQAAAEAGQRG
EEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PGSFQVAFLDALARIGVDDVKREGRVEQR
CCCEEHHHHHHHHHCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA