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The map label for this gene is glpF [H]

Identifier: 138894879

GI number: 138894879

Start: 1282935

End: 1283759

Strand: Direct

Name: glpF [H]

Synonym: GTNG_1215

Alternate gene names: 138894879

Gene position: 1282935-1283759 (Clockwise)

Preceding gene: 138894878

Following gene: 138894880

Centisome position: 36.14

GC content: 55.76

Gene sequence:

>825_bases
ATGTCACCATTTTTAGGAGAACTCGTCGGAACGGCGCTGCTCATCATTTTCGGATCCGGGGTGTGCGCCGGCGTGAACTT
AAAGAAATCGTATGCCGCGAATTCCGGTTGGATCGTGATTACGATGGGGTGGGGGCTAGCGGTCGCGATCGCAGCGTATG
CCGTCGGTCAATATAGCGGCGCTCATCTCAATCCGGCCTTAACCGTGGCGTTGGCCATAAGCGGCGACTTCCCATGGGAC
GATGTACTGATGTATGTCATTGCTCAGGTACATGGGGCGATGATTGGGGCGGCTGTTGTTTATCTCCACTATTGGCCGCA
TTGGAAAGAAACGGATGATCCTAGTATTAAGCTCGGTGTATTTGCTACTAGTCCGGCCGTGCCGAATGTGTCTGCTAACT
TGCTGAGCGAAATGATCGGTACGTTTGTGCTCGTCCTAGGGATTTTAGCCATTGGTGCCAACCGATTCGCCGATGGCCTC
AACCCGTTTATTGTCGGCTTTCTCATTATCGCCATCGGCTTGTCGCTCGGCGGTACGACCGGGTATGCCATCAACCCGGC
ACGTGATTTCGGACCGCGCCTCGCCCACTTTTTGTTGCCGATCCCGGGAAAAGGACCATCAAATTGGTCGTACGCCTGGG
TGCCGATTGTCGGGCCGCTGCTTGGCGGTGCGCTTGGCAGTCTAGTATATAAAGCGTTGTTTTTAGGAAAGACAAGTGCG
GCGCTATGGGGCGTGCTCATTGCCGCGGCGGCGGTGCTGGCTGGAGCTGGCATGATGAAAGGAAAAGGGGCGGGCTCGGC
GGTAGCGGATCGCCGTTCAGTTTGA

Upstream 100 bases:

>100_bases
GTGGGTCTTTGTTTTTTTGCCCAACGTGCAACCTTCCGGAAGGAGCACTGAAGCAGGAAATGGCAGAGGAAATCACGAAT
GAAAAGGGGGAAGAGACCAA

Downstream 100 bases:

>100_bases
TAGGAGGCCCGGTGTTTGCGGCGGTGTCATTGAGCTGAAAAAATCAAGTAGGAGAGGAGAATGTTCATGACAGATCAATA
TATTTTAGCGATTGACCAAG

Product: glycerol facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSPFLGELVGTALLIIFGSGVCAGVNLKKSYAANSGWIVITMGWGLAVAIAAYAVGQYSGAHLNPALTVALAISGDFPWD
DVLMYVIAQVHGAMIGAAVVYLHYWPHWKETDDPSIKLGVFATSPAVPNVSANLLSEMIGTFVLVLGILAIGANRFADGL
NPFIVGFLIIAIGLSLGGTTGYAINPARDFGPRLAHFLLPIPGKGPSNWSYAWVPIVGPLLGGALGSLVYKALFLGKTSA
ALWGVLIAAAAVLAGAGMMKGKGAGSAVADRRSV

Sequences:

>Translated_274_residues
MSPFLGELVGTALLIIFGSGVCAGVNLKKSYAANSGWIVITMGWGLAVAIAAYAVGQYSGAHLNPALTVALAISGDFPWD
DVLMYVIAQVHGAMIGAAVVYLHYWPHWKETDDPSIKLGVFATSPAVPNVSANLLSEMIGTFVLVLGILAIGANRFADGL
NPFIVGFLIIAIGLSLGGTTGYAINPARDFGPRLAHFLLPIPGKGPSNWSYAWVPIVGPLLGGALGSLVYKALFLGKTSA
ALWGVLIAAAAVLAGAGMMKGKGAGSAVADRRSV
>Mature_273_residues
SPFLGELVGTALLIIFGSGVCAGVNLKKSYAANSGWIVITMGWGLAVAIAAYAVGQYSGAHLNPALTVALAISGDFPWDD
VLMYVIAQVHGAMIGAAVVYLHYWPHWKETDDPSIKLGVFATSPAVPNVSANLLSEMIGTFVLVLGILAIGANRFADGLN
PFIVGFLIIAIGLSLGGTTGYAINPARDFGPRLAHFLLPIPGKGPSNWSYAWVPIVGPLLGGALGSLVYKALFLGKTSAA
LWGVLIAAAAVLAGAGMMKGKGAGSAVADRRSV

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=244, Percent_Identity=37.7049180327869, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI22538420, Length=245, Percent_Identity=35.1020408163265, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI157266307, Length=242, Percent_Identity=36.3636363636364, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4502187, Length=250, Percent_Identity=34.4, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI37694062, Length=240, Percent_Identity=32.0833333333333, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4502181, Length=207, Percent_Identity=31.8840579710145, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4755125, Length=206, Percent_Identity=32.0388349514563, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI6912506, Length=209, Percent_Identity=32.0574162679426, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4502179, Length=232, Percent_Identity=32.7586206896552, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI310133356, Length=183, Percent_Identity=30.0546448087432, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI310114181, Length=183, Percent_Identity=30.0546448087432, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI45446752, Length=220, Percent_Identity=27.7272727272727, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI86792455, Length=279, Percent_Identity=29.7491039426523, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790362, Length=249, Percent_Identity=36.5461847389558, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787101, Length=165, Percent_Identity=31.5151515151515, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71994009, Length=245, Percent_Identity=33.0612244897959, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17544068, Length=248, Percent_Identity=35.4838709677419, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71992966, Length=249, Percent_Identity=33.7349397590361, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17533613, Length=239, Percent_Identity=30.9623430962343, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32564052, Length=239, Percent_Identity=30.9623430962343, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531431, Length=240, Percent_Identity=32.0833333333333, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17531429, Length=240, Percent_Identity=32.0833333333333, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71992961, Length=237, Percent_Identity=32.0675105485232, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558372, Length=235, Percent_Identity=27.2340425531915, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71993722, Length=191, Percent_Identity=30.3664921465969, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=204, Percent_Identity=34.8039215686275, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322985, Length=306, Percent_Identity=28.4313725490196, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652747, Length=236, Percent_Identity=33.8983050847458, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45551084, Length=236, Percent_Identity=33.8983050847458, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24762342, Length=252, Percent_Identity=30.952380952381, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24762344, Length=249, Percent_Identity=30.5220883534137, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24762346, Length=249, Percent_Identity=30.5220883534137, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24762348, Length=249, Percent_Identity=30.5220883534137, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI20130305, Length=249, Percent_Identity=30.5220883534137, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922828, Length=229, Percent_Identity=28.3842794759825, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28176; Mature: 28045

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPFLGELVGTALLIIFGSGVCAGVNLKKSYAANSGWIVITMGWGLAVAIAAYAVGQYSG
CCCHHHHHHHHHHHHHHCCCCEECCCCHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPALTVALAISGDFPWDDVLMYVIAQVHGAMIGAAVVYLHYWPHWKETDDPSIKLGV
CCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
FATSPAVPNVSANLLSEMIGTFVLVLGILAIGANRFADGLNPFIVGFLIIAIGLSLGGTT
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
GYAINPARDFGPRLAHFLLPIPGKGPSNWSYAWVPIVGPLLGGALGSLVYKALFLGKTSA
CCEECCHHHHHHHHHHHHEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHH
ALWGVLIAAAAVLAGAGMMKGKGAGSAVADRRSV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
SPFLGELVGTALLIIFGSGVCAGVNLKKSYAANSGWIVITMGWGLAVAIAAYAVGQYSG
CCHHHHHHHHHHHHHHCCCCEECCCCHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPALTVALAISGDFPWDDVLMYVIAQVHGAMIGAAVVYLHYWPHWKETDDPSIKLGV
CCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
FATSPAVPNVSANLLSEMIGTFVLVLGILAIGANRFADGLNPFIVGFLIIAIGLSLGGTT
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
GYAINPARDFGPRLAHFLLPIPGKGPSNWSYAWVPIVGPLLGGALGSLVYKALFLGKTSA
CCEECCHHHHHHHHHHHHEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHH
ALWGVLIAAAAVLAGAGMMKGKGAGSAVADRRSV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]