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The map label for this gene is topA [H]

Identifier: 138894732

GI number: 138894732

Start: 1130665

End: 1132740

Strand: Direct

Name: topA [H]

Synonym: GTNG_1064

Alternate gene names: 138894732

Gene position: 1130665-1132740 (Clockwise)

Preceding gene: 138894731

Following gene: 138894733

Centisome position: 31.85

GC content: 50.63

Gene sequence:

>2076_bases
ATGTCAGACTACTTGGTTATCGTCGAATCGCCAACGAAAGCGAAGACGATCGAACGATACTTAGGGAAAAAATATACGGT
CAAAGCTTCGATGGGCCACGTTCGCGATTTGCCAAAAAGCCAAATGGGCGTTGACATAAATGACGGCTATACGCCGAAAT
ATATTACGATCCGCGGCAAAGGCCAAGTGATTAAAGAGTTGAAAACGGCAGCCAAAAAGGCGAAAAAAGTGTTTCTCGCT
GCCGACCCGGACCGTGAAGGGGAAGCAATCGCCTGGCATTTGGCCAACATGCTCGATCTCGATATTCACTCTGATTGCCG
CGTCGTCTTTCATGAGATTACAAAAGACGCCATTAAACAATCATTCCAGCAACCGCGCTCAATCAATATGAATCTCGTCG
ATGCCCAGCAAGCGCGGCGCGTGCTTGACCGTCTCGTCGGCTATAACATCAGCCCCCTCTTGTGGAAAAAAGTGAAAAAA
GGGCTGAGTGCCGGCCGCGTCCAATCAGTCGCGCTGCGTCTCATTATCGATCGGGAAAAAGAAATCCGCCAATTTCAACC
GGAAGAATATTGGACGATTCAAGCGAAATTTCAAAAAGGGAAAGAGGCGTTTGCCGCCTCGTTTTACGGCGTTGACGGGG
AAAAGCTGGAATTAAAAACGGAAGCGGACGTAAAAGAAGTGCTCGCCCGTTTAAACGGGCGAGCGTTTACGGTGAAAACG
GTGACAAAGCGCGAGCGCCGGCGCAGCCCGGTGCCACCGTTTACAACGTCATCGCTTCAGCAGGAAGCAGCGCGCAAGTT
GAATTTCCGCACAAAAAAGACGATGATGGTCGCCCAGCAGCTGTACGAAGGGATTGATCTCGGCGGAGAAGGGACAGTCG
GGTTGATAACGTATATGCGTACCGACTCGACGCGCATTGCCGAAACAGCGCAACAGGAAGCAGCGGCGTATATCGAAGCG
ACGTTCGGCGCCCAGTACGTCCATCAAGAAAAGCGGAAGGAAAAGAAAAGCACGAACGCCCAAGATGCTCACGAAGCCAT
TCGCCCGACCTCGGCATTTCGCGATCCGGATAAAGTGAAGCCATATTTGACGCGCGACCAGTTCCGGTTGTATAAACTCA
TTTGGGAACGGTTTATCGCCAGCCAAATGGCGGCGGCTGTACTGGACACGATGAGTGTTGAACTTGAAAATAATGGGGTG
CTGTTCCGCGCCAGCGGTTCGAAGGTAAAGTTCCCAGGGTTTATGAAAGTATACATCGAGGGGACGGACGACCAAACGGA
AGAACAAGATCGTCTGCTTCCTGATCTTGAGGAAGGAGAGGCGGTCGAAAGTGAAATGATCGAGCCGAAGCAGCATTTCA
CCCAGCCGCCGCCGCGCTATACAGAAGCGCGCCTTGTTAAAACGTTAGAAGAGCTCGGCATCGGCCGTCCGTCCACCTAC
GCGCCGACGCTTGATACGATTCAGAAACGCAACTACGTTGTTCTTGAAAACAAGCGGTTCGTCCCGACAGAGCTTGGTGA
AATCGTCGTCGAGCTTATTTTAGAATTTTTTCCAGAAATTATTGATGTCGAGTTTACGGCTAAAATGGAAAAAGAGCTTG
ATGAAATAGAAGAAGGAAAAGTAGAGTGGATCAAGGTTGTGGATGAATTTTACCGCGAGTTTGAGAAGCGGCTAAAAGTG
GCCGAAAAAGAAATGCGCGAAGTGGAAATTAAAGATGAGCCAGCCGGCATTGACTGCGACGTGTGCGGCAGTCCGATGGT
GTACAAAATGGGTCGCTTTGGTAAATTTATCGCTTGCTCGAACTTTCCGGAATGCCGCCATACGAAACCGATTGTGAAAG
AAATCGGCGTCAAATGCCCGAAATGCCATGAAGGGAATATTGTCGAGCGCAACAGCAAGCGAAAACGGGTGTTTTACGGC
TGCGATCGCTTCCCGGAATGCGACTTTGTTTCGTGGGATAAACCGCTTGCGCGCCCGTGTCCAAAGTGCGGAGGGCTGCT
TGTCGAGAAAAAGCTGAAAAAGGGTGTGCAAGTCCAATGCACGGCGTGCGACTATGAGGAACAACCAGAAGCTTGA

Upstream 100 bases:

>100_bases
CGCTGAATTCCCGTACGTATAATGATCATGTTTGACAAATACGGCAATAATCATTAATAATAATGAAGATTTCAATTTAC
TTCCTAGGGGTGGAAGATGG

Downstream 100 bases:

>100_bases
CCACGCATCGGATACAATTCAGAATTTTATAAAAAATAGTTGCGCGATGCAGAGTCATATGGTACGATGAAGGAGCCGTT
GCGAGGTGAGGCGATGAAAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MSDYLVIVESPTKAKTIERYLGKKYTVKASMGHVRDLPKSQMGVDINDGYTPKYITIRGKGQVIKELKTAAKKAKKVFLA
ADPDREGEAIAWHLANMLDLDIHSDCRVVFHEITKDAIKQSFQQPRSINMNLVDAQQARRVLDRLVGYNISPLLWKKVKK
GLSAGRVQSVALRLIIDREKEIRQFQPEEYWTIQAKFQKGKEAFAASFYGVDGEKLELKTEADVKEVLARLNGRAFTVKT
VTKRERRRSPVPPFTTSSLQQEAARKLNFRTKKTMMVAQQLYEGIDLGGEGTVGLITYMRTDSTRIAETAQQEAAAYIEA
TFGAQYVHQEKRKEKKSTNAQDAHEAIRPTSAFRDPDKVKPYLTRDQFRLYKLIWERFIASQMAAAVLDTMSVELENNGV
LFRASGSKVKFPGFMKVYIEGTDDQTEEQDRLLPDLEEGEAVESEMIEPKQHFTQPPPRYTEARLVKTLEELGIGRPSTY
APTLDTIQKRNYVVLENKRFVPTELGEIVVELILEFFPEIIDVEFTAKMEKELDEIEEGKVEWIKVVDEFYREFEKRLKV
AEKEMREVEIKDEPAGIDCDVCGSPMVYKMGRFGKFIACSNFPECRHTKPIVKEIGVKCPKCHEGNIVERNSKRKRVFYG
CDRFPECDFVSWDKPLARPCPKCGGLLVEKKLKKGVQVQCTACDYEEQPEA

Sequences:

>Translated_691_residues
MSDYLVIVESPTKAKTIERYLGKKYTVKASMGHVRDLPKSQMGVDINDGYTPKYITIRGKGQVIKELKTAAKKAKKVFLA
ADPDREGEAIAWHLANMLDLDIHSDCRVVFHEITKDAIKQSFQQPRSINMNLVDAQQARRVLDRLVGYNISPLLWKKVKK
GLSAGRVQSVALRLIIDREKEIRQFQPEEYWTIQAKFQKGKEAFAASFYGVDGEKLELKTEADVKEVLARLNGRAFTVKT
VTKRERRRSPVPPFTTSSLQQEAARKLNFRTKKTMMVAQQLYEGIDLGGEGTVGLITYMRTDSTRIAETAQQEAAAYIEA
TFGAQYVHQEKRKEKKSTNAQDAHEAIRPTSAFRDPDKVKPYLTRDQFRLYKLIWERFIASQMAAAVLDTMSVELENNGV
LFRASGSKVKFPGFMKVYIEGTDDQTEEQDRLLPDLEEGEAVESEMIEPKQHFTQPPPRYTEARLVKTLEELGIGRPSTY
APTLDTIQKRNYVVLENKRFVPTELGEIVVELILEFFPEIIDVEFTAKMEKELDEIEEGKVEWIKVVDEFYREFEKRLKV
AEKEMREVEIKDEPAGIDCDVCGSPMVYKMGRFGKFIACSNFPECRHTKPIVKEIGVKCPKCHEGNIVERNSKRKRVFYG
CDRFPECDFVSWDKPLARPCPKCGGLLVEKKLKKGVQVQCTACDYEEQPEA
>Mature_690_residues
SDYLVIVESPTKAKTIERYLGKKYTVKASMGHVRDLPKSQMGVDINDGYTPKYITIRGKGQVIKELKTAAKKAKKVFLAA
DPDREGEAIAWHLANMLDLDIHSDCRVVFHEITKDAIKQSFQQPRSINMNLVDAQQARRVLDRLVGYNISPLLWKKVKKG
LSAGRVQSVALRLIIDREKEIRQFQPEEYWTIQAKFQKGKEAFAASFYGVDGEKLELKTEADVKEVLARLNGRAFTVKTV
TKRERRRSPVPPFTTSSLQQEAARKLNFRTKKTMMVAQQLYEGIDLGGEGTVGLITYMRTDSTRIAETAQQEAAAYIEAT
FGAQYVHQEKRKEKKSTNAQDAHEAIRPTSAFRDPDKVKPYLTRDQFRLYKLIWERFIASQMAAAVLDTMSVELENNGVL
FRASGSKVKFPGFMKVYIEGTDDQTEEQDRLLPDLEEGEAVESEMIEPKQHFTQPPPRYTEARLVKTLEELGIGRPSTYA
PTLDTIQKRNYVVLENKRFVPTELGEIVVELILEFFPEIIDVEFTAKMEKELDEIEEGKVEWIKVVDEFYREFEKRLKVA
EKEMREVEIKDEPAGIDCDVCGSPMVYKMGRFGKFIACSNFPECRHTKPIVKEIGVKCPKCHEGNIVERNSKRKRVFYGC
DRFPECDFVSWDKPLARPCPKCGGLLVEKKLKKGVQVQCTACDYEEQPEA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=663, Percent_Identity=24.4343891402715, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI10835218, Length=584, Percent_Identity=24.3150684931507, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1787529, Length=756, Percent_Identity=38.0952380952381, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI48994931, Length=107, Percent_Identity=45.7943925233645, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788061, Length=544, Percent_Identity=23.8970588235294, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=568, Percent_Identity=26.2323943661972, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=541, Percent_Identity=24.9537892791128, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=578, Percent_Identity=25.0865051903114, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24585251, Length=652, Percent_Identity=23.159509202454, Blast_Score=128, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640096, Length=525, Percent_Identity=23.2380952380952, Blast_Score=111, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79043; Mature: 78912

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYLVIVESPTKAKTIERYLGKKYTVKASMGHVRDLPKSQMGVDINDGYTPKYITIRGK
CCCEEEEEECCCHHHHHHHHHCCEEEEEECCCHHHCCCHHHCCCCCCCCCCCCEEEEECC
GQVIKELKTAAKKAKKVFLAADPDREGEAIAWHLANMLDLDIHSDCRVVFHEITKDAIKQ
HHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SFQQPRSINMNLVDAQQARRVLDRLVGYNISPLLWKKVKKGLSAGRVQSVALRLIIDREK
HHCCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
EIRQFQPEEYWTIQAKFQKGKEAFAASFYGVDGEKLELKTEADVKEVLARLNGRAFTVKT
HHHHCCCCCEEEEEEHHHCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEH
VTKRERRRSPVPPFTTSSLQQEAARKLNFRTKKTMMVAQQLYEGIDLGGEGTVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
TDSTRIAETAQQEAAAYIEATFGAQYVHQEKRKEKKSTNAQDAHEAIRPTSAFRDPDKVK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCCCHHHCC
PYLTRDQFRLYKLIWERFIASQMAAAVLDTMSVELENNGVLFRASGSKVKFPGFMKVYIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCEEECCCEEEEEEE
GTDDQTEEQDRLLPDLEEGEAVESEMIEPKQHFTQPPPRYTEARLVKTLEELGIGRPSTY
CCCCCCCHHHHCCCCCCCCCHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
APTLDTIQKRNYVVLENKRFVPTELGEIVVELILEFFPEIIDVEFTAKMEKELDEIEEGK
CCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
VEWIKVVDEFYREFEKRLKVAEKEMREVEIKDEPAGIDCDVCGSPMVYKMGRFGKFIACS
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHCCCCCEEEEC
NFPECRHTKPIVKEIGVKCPKCHEGNIVERNSKRKRVFYGCDRFPECDFVSWDKPLARPC
CCCCCCCCCHHHHHHCCCCCCCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCCHHCCC
PKCGGLLVEKKLKKGVQVQCTACDYEEQPEA
CHHCCHHHHHHHHCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
SDYLVIVESPTKAKTIERYLGKKYTVKASMGHVRDLPKSQMGVDINDGYTPKYITIRGK
CCEEEEEECCCHHHHHHHHHCCEEEEEECCCHHHCCCHHHCCCCCCCCCCCCEEEEECC
GQVIKELKTAAKKAKKVFLAADPDREGEAIAWHLANMLDLDIHSDCRVVFHEITKDAIKQ
HHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SFQQPRSINMNLVDAQQARRVLDRLVGYNISPLLWKKVKKGLSAGRVQSVALRLIIDREK
HHCCCCCCCCEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
EIRQFQPEEYWTIQAKFQKGKEAFAASFYGVDGEKLELKTEADVKEVLARLNGRAFTVKT
HHHHCCCCCEEEEEEHHHCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEEH
VTKRERRRSPVPPFTTSSLQQEAARKLNFRTKKTMMVAQQLYEGIDLGGEGTVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
TDSTRIAETAQQEAAAYIEATFGAQYVHQEKRKEKKSTNAQDAHEAIRPTSAFRDPDKVK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHCCCCHHHCC
PYLTRDQFRLYKLIWERFIASQMAAAVLDTMSVELENNGVLFRASGSKVKFPGFMKVYIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCEEECCCEEEEEEE
GTDDQTEEQDRLLPDLEEGEAVESEMIEPKQHFTQPPPRYTEARLVKTLEELGIGRPSTY
CCCCCCCHHHHCCCCCCCCCHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
APTLDTIQKRNYVVLENKRFVPTELGEIVVELILEFFPEIIDVEFTAKMEKELDEIEEGK
CCCHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
VEWIKVVDEFYREFEKRLKVAEKEMREVEIKDEPAGIDCDVCGSPMVYKMGRFGKFIACS
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHCCCCCEEEEC
NFPECRHTKPIVKEIGVKCPKCHEGNIVERNSKRKRVFYGCDRFPECDFVSWDKPLARPC
CCCCCCCCCHHHHHHCCCCCCCCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCCHHCCC
PKCGGLLVEKKLKKGVQVQCTACDYEEQPEA
CHHCCHHHHHHHHCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]