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The map label for this gene is trmD [H]

Identifier: 138894722

GI number: 138894722

Start: 1121893

End: 1122630

Strand: Direct

Name: trmD [H]

Synonym: GTNG_1054

Alternate gene names: 138894722

Gene position: 1121893-1122630 (Clockwise)

Preceding gene: 138894721

Following gene: 138894723

Centisome position: 31.6

GC content: 51.49

Gene sequence:

>738_bases
ATGATGCGCATCGATATTTTAACGCTGTTTCCCGACATGTTTCCCGGCGTGCTGAATGAGTCGATTTTAAAGAAGGCGCA
AGAAAAGGGAGCAGTGAACATCCGTCTCGTTAACTTCCGCGAATTTGCCGACAACAAGCATAAAACGGTCGATGATTATC
CATACGGCGGCGGTGCCGGCATGGTATTGAAGCCGCAGCCGATTTTTGATGCGGTGGAGCATGTGACGGCGGAGTCGCCA
GGCGCGCGCATCATTTTGCTTTGCCCGCAAGGTGAGCGCTACACGCAAAAAAAGGCGGAGGAGCTCGCCAAAGAAGAACA
CTTGGTGCTTATTTGCGGCCATTATGAGGGTTATGACGAACGAATTCGCCAATATTTAGTGACCGACGAAATTTCAATTG
GTGATTACATTTTAACCGGTGGAGAGCTCGGCGCCATGGTGATTGTCGACAGCGTTGTTCGCCTCCTTCCTGGCGTTCTC
GGCAACGAGGCATCACCGGTTGATGACTCATTTAGTTCTGGACTGCTTGAGTACCCACAATACACAAGGCCGGCTGATTT
TCGCGGCATGAAAGTGCCGGATGTGTTGCTGTCAGGAAACCATCAGCTCATCGCCGAATGGCGCGAGAAGGAATCGCTTC
GCCGGACGGTGCAAAAACGTCCGGATTTGCTTGTCGATTATCCGCTCACCGAGAAGCAAAAGCAATGGCTGAAAGAGTGG
GAGCAAGAGCGAGAATAA

Upstream 100 bases:

>100_bases
ACGGCACCGACGCACTCATTCCGTATATTGACGATGTCGTCATGCACGTCGACCCTGAACGGAAGATAATCATCATCCGG
CCGATGGAAGGGCTGCTTGA

Downstream 100 bases:

>100_bases
CTATTGCTTGCAGTCGATGTTTATGCTATCATATTCTTTGTGTTGCTTAAGCGGTATGCTTAAGGATGGAAAACGATGTT
CCGCTGCAATGAATGAGACG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MMRIDILTLFPDMFPGVLNESILKKAQEKGAVNIRLVNFREFADNKHKTVDDYPYGGGAGMVLKPQPIFDAVEHVTAESP
GARIILLCPQGERYTQKKAEELAKEEHLVLICGHYEGYDERIRQYLVTDEISIGDYILTGGELGAMVIVDSVVRLLPGVL
GNEASPVDDSFSSGLLEYPQYTRPADFRGMKVPDVLLSGNHQLIAEWREKESLRRTVQKRPDLLVDYPLTEKQKQWLKEW
EQERE

Sequences:

>Translated_245_residues
MMRIDILTLFPDMFPGVLNESILKKAQEKGAVNIRLVNFREFADNKHKTVDDYPYGGGAGMVLKPQPIFDAVEHVTAESP
GARIILLCPQGERYTQKKAEELAKEEHLVLICGHYEGYDERIRQYLVTDEISIGDYILTGGELGAMVIVDSVVRLLPGVL
GNEASPVDDSFSSGLLEYPQYTRPADFRGMKVPDVLLSGNHQLIAEWREKESLRRTVQKRPDLLVDYPLTEKQKQWLKEW
EQERE
>Mature_245_residues
MMRIDILTLFPDMFPGVLNESILKKAQEKGAVNIRLVNFREFADNKHKTVDDYPYGGGAGMVLKPQPIFDAVEHVTAESP
GARIILLCPQGERYTQKKAEELAKEEHLVLICGHYEGYDERIRQYLVTDEISIGDYILTGGELGAMVIVDSVVRLLPGVL
GNEASPVDDSFSSGLLEYPQYTRPADFRGMKVPDVLLSGNHQLIAEWREKESLRRTVQKRPDLLVDYPLTEKQKQWLKEW
EQERE

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=243, Percent_Identity=47.7366255144033, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27889; Mature: 27889

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIDILTLFPDMFPGVLNESILKKAQEKGAVNIRLVNFREFADNKHKTVDDYPYGGGAG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCC
MVLKPQPIFDAVEHVTAESPGARIILLCPQGERYTQKKAEELAKEEHLVLICGHYEGYDE
EEECCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
RIRQYLVTDEISIGDYILTGGELGAMVIVDSVVRLLPGVLGNEASPVDDSFSSGLLEYPQ
HHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCC
YTRPADFRGMKVPDVLLSGNHQLIAEWREKESLRRTVQKRPDLLVDYPLTEKQKQWLKEW
CCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
EQERE
HHHCC
>Mature Secondary Structure
MMRIDILTLFPDMFPGVLNESILKKAQEKGAVNIRLVNFREFADNKHKTVDDYPYGGGAG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCC
MVLKPQPIFDAVEHVTAESPGARIILLCPQGERYTQKKAEELAKEEHLVLICGHYEGYDE
EEECCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
RIRQYLVTDEISIGDYILTGGELGAMVIVDSVVRLLPGVLGNEASPVDDSFSSGLLEYPQ
HHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCC
YTRPADFRGMKVPDVLLSGNHQLIAEWREKESLRRTVQKRPDLLVDYPLTEKQKQWLKEW
CCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHH
EQERE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA