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The map label for this gene is def1 [H]

Identifier: 138894692

GI number: 138894692

Start: 1093595

End: 1094068

Strand: Direct

Name: def1 [H]

Synonym: GTNG_1024

Alternate gene names: 138894692

Gene position: 1093595-1094068 (Clockwise)

Preceding gene: 138894691

Following gene: 138894693

Centisome position: 30.8

GC content: 53.59

Gene sequence:

>474_bases
TTGTCTGTATTGCCGATTGTCACCTATCCTGCTCCAGTTTTGGAACAACCGTGCGCGCGGGTGACGGTCTTTGACCGCCA
GCTCGGCCGATTGCTTGACGATATGTATGAGACGATGATTGCCGCTGATGGAGTTGGCTTGGCCGCCCCGCAAGTTGGCA
TTGCCAAACAGGTTGCTGTCGTCGATATCGGCGATGAGCATGGCCGGATTGAGCTTATCAATCCAGTTATCATCGAAGCG
CGCGGTGAACAAATTGATGTTGAAGGCTGCTTGAGCTTTCCTGGTCTGTTTGGCGAAGTGCCGCGCGCCAATTATGTGAA
AGTGCGGGCGCAAAACCGGCGCGGCCGTCTGTTTACACTCTCTGCAACCGGCTTTTTAGCGCGCGCCTTGCAGCACGAAA
TCGATCACTTGCACGGCGTGTTGTTTACATCGAAAGTGATCCGTTACTGCGATCTTGAAGAATTGGAAGGGTAG

Upstream 100 bases:

>100_bases
GCGGTCATCGATCGTTATCAAGCGGATGCCACCCACGGTGGGGTGGCGATTACCGTCGATACGAACCCGTATATGATGAT
GTGAAAATGGAGGAATGACG

Downstream 100 bases:

>100_bases
ATGCTGATGACGAACATTGTCTTTATGGGAACGCCTGATTTTGCGGTGCCGGTTTTACGGCAGCTGCTTGATGACGGGTA
TCGGGTTGTTGCCGTTGTTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MSVLPIVTYPAPVLEQPCARVTVFDRQLGRLLDDMYETMIAADGVGLAAPQVGIAKQVAVVDIGDEHGRIELINPVIIEA
RGEQIDVEGCLSFPGLFGEVPRANYVKVRAQNRRGRLFTLSATGFLARALQHEIDHLHGVLFTSKVIRYCDLEELEG

Sequences:

>Translated_157_residues
MSVLPIVTYPAPVLEQPCARVTVFDRQLGRLLDDMYETMIAADGVGLAAPQVGIAKQVAVVDIGDEHGRIELINPVIIEA
RGEQIDVEGCLSFPGLFGEVPRANYVKVRAQNRRGRLFTLSATGFLARALQHEIDHLHGVLFTSKVIRYCDLEELEG
>Mature_156_residues
SVLPIVTYPAPVLEQPCARVTVFDRQLGRLLDDMYETMIAADGVGLAAPQVGIAKQVAVVDIGDEHGRIELINPVIIEAR
GEQIDVEGCLSFPGLFGEVPRANYVKVRAQNRRGRLFTLSATGFLARALQHEIDHLHGVLFTSKVIRYCDLEELEG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=143, Percent_Identity=44.7552447552448, Blast_Score=117, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=30.379746835443, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 17268; Mature: 17137

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLPIVTYPAPVLEQPCARVTVFDRQLGRLLDDMYETMIAADGVGLAAPQVGIAKQVAV
CCCCCEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
VDIGDEHGRIELINPVIIEARGEQIDVEGCLSFPGLFGEVPRANYVKVRAQNRRGRLFTL
EEECCCCCEEEEECEEEEEECCCEECHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEEEE
SATGFLARALQHEIDHLHGVLFTSKVIRYCDLEELEG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
SVLPIVTYPAPVLEQPCARVTVFDRQLGRLLDDMYETMIAADGVGLAAPQVGIAKQVAV
CCCCEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
VDIGDEHGRIELINPVIIEARGEQIDVEGCLSFPGLFGEVPRANYVKVRAQNRRGRLFTL
EEECCCCCEEEEECEEEEEECCCEECHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEEEE
SATGFLARALQHEIDHLHGVLFTSKVIRYCDLEELEG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]