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The map label for this gene is priA [H]

Identifier: 138894691

GI number: 138894691

Start: 1091173

End: 1093578

Strand: Direct

Name: priA [H]

Synonym: GTNG_1023

Alternate gene names: 138894691

Gene position: 1091173-1093578 (Clockwise)

Preceding gene: 138894690

Following gene: 138894692

Centisome position: 30.73

GC content: 55.69

Gene sequence:

>2406_bases
ATGAAGGTTGCATCGGTCATTGTCGATGTCCCGACGCGGCAAACAGACCGCCCGTTCGACTATTTGATTCCTGAGCGCTG
GCGCGGCATCATCCAGCCGGGAATGCGGGTAACGGTGCCGTTCGGAGCGCGGCGTCTGCAAGGGTTTGTCATTGGAGTAA
AAGATCACTCTGAGATCAAGCAGTTAAAGCCGATTGAACATGTACTTGATGTCGTGCCAGTATTAAATGAAGAACTGCTT
GACCTCGGCCGCTATTTAACGGAAACGACGCTCTGTTTTGCCATTTCGGCGTATCAAGCGATGTTGCCGGCGGCGATGCG
GGCGAAGTATGAAAAGCTGCTCCGCCTAACGGATGAACGAAAGCGCGAGGCGCTGCCGGACGACTTAAAGCCGCTGTTTG
CCAACCGAGTAGAAGTCGCTTGGAAAGACGTCGAAACGGCCGGCTTATGGCGGGCGGCACAAAGGGCAGTGCAGCAAGGC
GTGCTTGAAGCGGTCTACGAAGTGAAAGAAAAAGCTGGGAAGAAAACGGTGAAGCATGTTGTTCTTGCTGTTCCAGCAGA
GCAGGCGAAAGACGCGCTGGCAGCGCTTCCGGCAAGGCAAAAAGACGTGCTTTCGTTTTTGCTTGAAAAAGAGGAAGCGG
TGCCGGTCGCCAAGCTACAAGCGGCGCTTGGTGTTTCCTCTGCACCGCTTAAAGCGCTCGTCGGCAAAGGGCTGATTGCG
ATGAGCGATGTTGAAGTTTATCGCGATCCGTACGAACATCGTACGTTTCAGCCAAGCGAACCGCCGACGCTCACCCAAGC
GCAGACCAAGGCGTTGGCGGCAATTACCGCTTCGGTGCGCGCTAGGGAGCACCGTACGTTTTTGCTTTACGGCGTCACCG
GCAGTGGGAAAACGGAAGTGTATATGCAGGCGATTGAAGAAGTGTTGCGCCAAGGGAAAGAGGCGATCGTTCTCGTGCCG
GAAATCTCGCTGACGCCGCAAATGGTCGAGCGCTTTAAAGGCCGGTTCGGCCCGAAAGTAGCGGTGCTCCACAGCGGGCT
GTCAGTCGGCGAAAAATATGATGAATGGCGGAAAATCCATCGGAAAGAGGTACAGCTTGTTGTCGGTGCGCGTTCCGCAG
TATTTGCCCCGTTTGAAAACTTAGGGATGATCATTATCGACGAGGAACATGAGACGAGCTATAAGCAAGAAGAAATGCCG
CGCTACCATGCGCGGGATGTTGCCATTTACCGCGCCCGCCGCCACCGCTGTCCGGTCGTGCTCGGCAGCGCCACTCCATC
GCTTGAAACGTTCGCACGTGCCGTCAAAGGGGTGTATGAGCTGCTTGAACTGCCGGAGCGGATTGCTGATCGCGGTATGC
CGGATGTCCATATTGTCGATATGCGCGAGGAATTGCGCAGCGGCAATCGTTCGATGTTTTCACGGCGATTGCTTGATGAG
CTGCGTCTTCGACTGGAACGCGGCGAGCAGGCGGTATTGTTTTTAAATCGACGCGGCTATTCGACGTTTGTCATGTGCCG
CGGCTGTGGCTATGTCATCTGTTGTCCGCATTGCGACATTTCGCTTACGTATCATCGCGCGGGAGAGAGGATGAAATGCC
ATTATTGCGGTTACGAAGAGCCGCTAGTTCCTCGCTGTCCATCGTGCGGAAGCGAACATATCCGCTTTTTCGGCACTGGC
ACGCAAAAAGTGGAAGAAGAGTTGGTCAAGCTGTTGCCGAAGGCGCGCATCATCCGCATGGATGTCGATACGACGGGGCG
GAAAGGGGCGCATGAAGAGCTGCTCTCCCGCTTTGCTGCCAAAGAGGCGGATATTTTGCTCGGCACGCAAATGATCGCCA
AGGGGCTTGATTTTCCTGACGTGACGCTCGTCGGTGTGCTTGCGGCCGATACGATGCTTCATCTGCCCGATTTTCGCGCC
GCAGAGAAAACATTTCAGCTGCTGACGCAAGTGAGCGGGCGCGCCGGACGGCATGAGCTTCCCGGTGAAGTCGTCATCCA
GACGTATACGCCGGATCACTACAGCATTGAGCTCGCCGCCCGCCACGATTATCGCGCCTTTTACCGACGGGAGATGACGC
TGCGCAAAGCGCATGGCTATCCGCCATACTACTATTTGACGCTCATTACCGTCGCTCACGAGGAAGCGCCGGCCGCCGCC
AAAGCGGCCGAAAAAATCGCTGCCTATTTGCGCAAACAGCTGTCGAATGAAGCGGTCGTCCTCGGCCCGGTCGCCTCACC
GATCGCTCGCCTTCATGATAGATATCGTTACCAATGCATGATAAAATACAAACGGGAACCGAACATAACGGCGGCGCTGA
AAGCGGTCATCGATCGTTATCAAGCGGATGCCACCCACGGTGGGGTGGCGATTACCGTCGATACGAACCCGTATATGATG
ATGTGA

Upstream 100 bases:

>100_bases
TCCGCCGCGACGGCGCCACTCGTCCGTTGCCGCTCATGACGAAACGAGAGGCCGCTGAGGAAATTTTAAAAGAAATTCAC
GCGTATACCGAGGGGATTCG

Downstream 100 bases:

>100_bases
AAATGGAGGAATGACGTTGTCTGTATTGCCGATTGTCACCTATCCTGCTCCAGTTTTGGAACAACCGTGCGCGCGGGTGA
CGGTCTTTGACCGCCAGCTC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKVASVIVDVPTRQTDRPFDYLIPERWRGIIQPGMRVTVPFGARRLQGFVIGVKDHSEIKQLKPIEHVLDVVPVLNEELL
DLGRYLTETTLCFAISAYQAMLPAAMRAKYEKLLRLTDERKREALPDDLKPLFANRVEVAWKDVETAGLWRAAQRAVQQG
VLEAVYEVKEKAGKKTVKHVVLAVPAEQAKDALAALPARQKDVLSFLLEKEEAVPVAKLQAALGVSSAPLKALVGKGLIA
MSDVEVYRDPYEHRTFQPSEPPTLTQAQTKALAAITASVRAREHRTFLLYGVTGSGKTEVYMQAIEEVLRQGKEAIVLVP
EISLTPQMVERFKGRFGPKVAVLHSGLSVGEKYDEWRKIHRKEVQLVVGARSAVFAPFENLGMIIIDEEHETSYKQEEMP
RYHARDVAIYRARRHRCPVVLGSATPSLETFARAVKGVYELLELPERIADRGMPDVHIVDMREELRSGNRSMFSRRLLDE
LRLRLERGEQAVLFLNRRGYSTFVMCRGCGYVICCPHCDISLTYHRAGERMKCHYCGYEEPLVPRCPSCGSEHIRFFGTG
TQKVEEELVKLLPKARIIRMDVDTTGRKGAHEELLSRFAAKEADILLGTQMIAKGLDFPDVTLVGVLAADTMLHLPDFRA
AEKTFQLLTQVSGRAGRHELPGEVVIQTYTPDHYSIELAARHDYRAFYRREMTLRKAHGYPPYYYLTLITVAHEEAPAAA
KAAEKIAAYLRKQLSNEAVVLGPVASPIARLHDRYRYQCMIKYKREPNITAALKAVIDRYQADATHGGVAITVDTNPYMM
M

Sequences:

>Translated_801_residues
MKVASVIVDVPTRQTDRPFDYLIPERWRGIIQPGMRVTVPFGARRLQGFVIGVKDHSEIKQLKPIEHVLDVVPVLNEELL
DLGRYLTETTLCFAISAYQAMLPAAMRAKYEKLLRLTDERKREALPDDLKPLFANRVEVAWKDVETAGLWRAAQRAVQQG
VLEAVYEVKEKAGKKTVKHVVLAVPAEQAKDALAALPARQKDVLSFLLEKEEAVPVAKLQAALGVSSAPLKALVGKGLIA
MSDVEVYRDPYEHRTFQPSEPPTLTQAQTKALAAITASVRAREHRTFLLYGVTGSGKTEVYMQAIEEVLRQGKEAIVLVP
EISLTPQMVERFKGRFGPKVAVLHSGLSVGEKYDEWRKIHRKEVQLVVGARSAVFAPFENLGMIIIDEEHETSYKQEEMP
RYHARDVAIYRARRHRCPVVLGSATPSLETFARAVKGVYELLELPERIADRGMPDVHIVDMREELRSGNRSMFSRRLLDE
LRLRLERGEQAVLFLNRRGYSTFVMCRGCGYVICCPHCDISLTYHRAGERMKCHYCGYEEPLVPRCPSCGSEHIRFFGTG
TQKVEEELVKLLPKARIIRMDVDTTGRKGAHEELLSRFAAKEADILLGTQMIAKGLDFPDVTLVGVLAADTMLHLPDFRA
AEKTFQLLTQVSGRAGRHELPGEVVIQTYTPDHYSIELAARHDYRAFYRREMTLRKAHGYPPYYYLTLITVAHEEAPAAA
KAAEKIAAYLRKQLSNEAVVLGPVASPIARLHDRYRYQCMIKYKREPNITAALKAVIDRYQADATHGGVAITVDTNPYMM
M
>Mature_801_residues
MKVASVIVDVPTRQTDRPFDYLIPERWRGIIQPGMRVTVPFGARRLQGFVIGVKDHSEIKQLKPIEHVLDVVPVLNEELL
DLGRYLTETTLCFAISAYQAMLPAAMRAKYEKLLRLTDERKREALPDDLKPLFANRVEVAWKDVETAGLWRAAQRAVQQG
VLEAVYEVKEKAGKKTVKHVVLAVPAEQAKDALAALPARQKDVLSFLLEKEEAVPVAKLQAALGVSSAPLKALVGKGLIA
MSDVEVYRDPYEHRTFQPSEPPTLTQAQTKALAAITASVRAREHRTFLLYGVTGSGKTEVYMQAIEEVLRQGKEAIVLVP
EISLTPQMVERFKGRFGPKVAVLHSGLSVGEKYDEWRKIHRKEVQLVVGARSAVFAPFENLGMIIIDEEHETSYKQEEMP
RYHARDVAIYRARRHRCPVVLGSATPSLETFARAVKGVYELLELPERIADRGMPDVHIVDMREELRSGNRSMFSRRLLDE
LRLRLERGEQAVLFLNRRGYSTFVMCRGCGYVICCPHCDISLTYHRAGERMKCHYCGYEEPLVPRCPSCGSEHIRFFGTG
TQKVEEELVKLLPKARIIRMDVDTTGRKGAHEELLSRFAAKEADILLGTQMIAKGLDFPDVTLVGVLAADTMLHLPDFRA
AEKTFQLLTQVSGRAGRHELPGEVVIQTYTPDHYSIELAARHDYRAFYRREMTLRKAHGYPPYYYLTLITVAHEEAPAAA
KAAEKIAAYLRKQLSNEAVVLGPVASPIARLHDRYRYQCMIKYKREPNITAALKAVIDRYQADATHGGVAITVDTNPYMM
M

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=548, Percent_Identity=40.6934306569343, Blast_Score=407, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 90279; Mature: 90279

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVASVIVDVPTRQTDRPFDYLIPERWRGIIQPGMRVTVPFGARRLQGFVIGVKDHSEIK
CCHHEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCEECEEEEEECCHHHHH
QLKPIEHVLDVVPVLNEELLDLGRYLTETTLCFAISAYQAMLPAAMRAKYEKLLRLTDER
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KREALPDDLKPLFANRVEVAWKDVETAGLWRAAQRAVQQGVLEAVYEVKEKAGKKTVKHV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAVPAEQAKDALAALPARQKDVLSFLLEKEEAVPVAKLQAALGVSSAPLKALVGKGLIA
HEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCEE
MSDVEVYRDPYEHRTFQPSEPPTLTQAQTKALAAITASVRAREHRTFLLYGVTGSGKTEV
EECHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
YMQAIEEVLRQGKEAIVLVPEISLTPQMVERFKGRFGPKVAVLHSGLSVGEKYDEWRKIH
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
RKEVQLVVGARSAVFAPFENLGMIIIDEEHETSYKQEEMPRYHARDVAIYRARRHRCPVV
HHHHHEEEECCHHHCCCHHHCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCEE
LGSATPSLETFARAVKGVYELLELPERIADRGMPDVHIVDMREELRSGNRSMFSRRLLDE
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCHHHHHHHHHHH
LRLRLERGEQAVLFLNRRGYSTFVMCRGCGYVICCPHCDISLTYHRAGERMKCHYCGYEE
HHHHHHCCCEEEEEEECCCCEEEEEECCCCEEEECCCCCEEEEEECCCCCEEEEECCCCC
PLVPRCPSCGSEHIRFFGTGTQKVEEELVKLLPKARIIRMDVDTTGRKGAHEELLSRFAA
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHH
KEADILLGTQMIAKGLDFPDVTLVGVLAADTMLHLPDFRAAEKTFQLLTQVSGRAGRHEL
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
PGEVVIQTYTPDHYSIELAARHDYRAFYRREMTLRKAHGYPPYYYLTLITVAHEEAPAAA
CCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCCHHH
KAAEKIAAYLRKQLSNEAVVLGPVASPIARLHDRYRYQCMIKYKREPNITAALKAVIDRY
HHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHH
QADATHGGVAITVDTNPYMMM
HCCCCCCCEEEEECCCCCEEC
>Mature Secondary Structure
MKVASVIVDVPTRQTDRPFDYLIPERWRGIIQPGMRVTVPFGARRLQGFVIGVKDHSEIK
CCHHEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCEECEEEEEECCHHHHH
QLKPIEHVLDVVPVLNEELLDLGRYLTETTLCFAISAYQAMLPAAMRAKYEKLLRLTDER
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KREALPDDLKPLFANRVEVAWKDVETAGLWRAAQRAVQQGVLEAVYEVKEKAGKKTVKHV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAVPAEQAKDALAALPARQKDVLSFLLEKEEAVPVAKLQAALGVSSAPLKALVGKGLIA
HEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCEE
MSDVEVYRDPYEHRTFQPSEPPTLTQAQTKALAAITASVRAREHRTFLLYGVTGSGKTEV
EECHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
YMQAIEEVLRQGKEAIVLVPEISLTPQMVERFKGRFGPKVAVLHSGLSVGEKYDEWRKIH
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
RKEVQLVVGARSAVFAPFENLGMIIIDEEHETSYKQEEMPRYHARDVAIYRARRHRCPVV
HHHHHEEEECCHHHCCCHHHCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCEE
LGSATPSLETFARAVKGVYELLELPERIADRGMPDVHIVDMREELRSGNRSMFSRRLLDE
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCHHHHHHHHHHH
LRLRLERGEQAVLFLNRRGYSTFVMCRGCGYVICCPHCDISLTYHRAGERMKCHYCGYEE
HHHHHHCCCEEEEEEECCCCEEEEEECCCCEEEECCCCCEEEEEECCCCCEEEEECCCCC
PLVPRCPSCGSEHIRFFGTGTQKVEEELVKLLPKARIIRMDVDTTGRKGAHEELLSRFAA
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHH
KEADILLGTQMIAKGLDFPDVTLVGVLAADTMLHLPDFRAAEKTFQLLTQVSGRAGRHEL
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCC
PGEVVIQTYTPDHYSIELAARHDYRAFYRREMTLRKAHGYPPYYYLTLITVAHEEAPAAA
CCCEEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCCHHH
KAAEKIAAYLRKQLSNEAVVLGPVASPIARLHDRYRYQCMIKYKREPNITAALKAVIDRY
HHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHH
QADATHGGVAITVDTNPYMMM
HCCCCCCCEEEEECCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]