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The map label for this gene is pbpB [H]

Identifier: 138894648

GI number: 138894648

Start: 1042746

End: 1044956

Strand: Direct

Name: pbpB [H]

Synonym: GTNG_0978

Alternate gene names: 138894648

Gene position: 1042746-1044956 (Clockwise)

Preceding gene: 138894647

Following gene: 138894649

Centisome position: 29.37

GC content: 50.88

Gene sequence:

>2211_bases
ATGGAAATGAAAAAACATTCCAACACCCATCGAGGAGCAGGGATTTTGTTTATCGTTTTCAGCCTGCTCTTTTTTCTGCT
CTTTGCCCGTTTTATCCAACTGCAATGGACCGGAAAAGCGGACGGTCATGTGTTAGCCGCGAAAGCTGAACAGCAGCATA
AACAAAAGCGAGTGATCGAAGCGAAGCGCGGGACGATTTTCGATCGCAACGGCACCATTTTGGCGCAGGATGTACCTTCA
TATACGGTCGTGGCCATTTTGGATCCAGAGATGACGACCAACCCGGAACGTCCGCGTCATGTTGTCGATCCGGAAACAAC
GGCGAAAAAGCTTGCGCCGTTGCTTGGTATGGATGTCAAGGACATGGAGCGCATTTTAACGAAAAAGGCCAAGCAGGTCG
AGTTCGGCTCCTACGGCCGCAACATCAGCTTTGAACTCAAGCGAAAAATTGAAGCGCTCGATTTGCCGGGCATTGCCTTC
ATCCGTGATACAAATCGGTTTTATCCGAATGGCACATTTGCTTCGCACGTGATCGGCTACGCACAAAAAAATGCGGAAAA
CGAAACAGTGGGCAAAATGGGTGTGGAAAAATGGCTTGATCGATATTTGCGCGAAACAGACGGCTATGTGTCATTTGCTG
GTGATGTGAACGGCTTTCGCCTGCCGGAGGCAAAAGAACGTATCGTCAAGCCAGACAACGGGGCGAATGTCTATTTGACG
ATCGATGGAAAAATCCAAACATTTTTAGAAGACGCCATGAATAACGTTGAGAAGCAGTACAAGCCGAAGAAAATGATTGG
TATCGTCGCTGATCCGAAAACAGGGAAAATTTTGGCTATGGCGACCCGTCCGAGTTTTGATCCGAATAAGCGCGACATTA
CGAACTTTTTGAATGATGCCATTTCCTATCCGTACGAGCCAGGGTCGACGATGAAGGTGTTTACGCTCGCGGCGGCTATT
AATGAAGGAGTATATAACGGCAAGGAAACGTTTCGTTCCGGGGCGTACAAAGTTGGGCCAAACACTGTTCGCGACCACAA
CGATGTTGGCTGGGGAACAATTACATTTGACGAAGGAGTGCAGCGTTCATCGAACGTCGCTTTTTCCATTTTAGTGAAAG
AAAAGCTCGGTGAAGATCGATTTTTGCAATATTTGCATCGCTTCCGCTTCGACCAGAAAACCGGCATCGATTTGCCGAAC
GAGTCTGTTGGGCAAATCCGCTATCGCTATCCGATTGAACGAATAACGACAGGATTTGGCCAAGGGACGTCGGTGACGCC
GATTCAGCAAATTCAAGCAGCAACGGCGATCGCTAATGATGGAAAAATGATGAAGCCATACATCATTGAAAAAGTGGTTG
ATCCGGACAACGGCAAAGTCGTTCTTGATCATGAGCCGACTGTAGCCGGTGAACCGATCACTGCGGAGACGGCGCGCCAA
GTGCGCGAGATTTTAGAGACGGTTGTCTCGTCGGAACACGGTACCGGCCGTCCGTACCAGATCGATGGTTACCGTGTCGC
AGGCAAAACAGGGACGGCGCAAATTCCGTCGGCCGCCGGCGGCTACATGACGGGCCGGGAAAACTATATTTTTTCGTTTT
TAGGTATGGCGCCGGCTGACGATCCACGGCTTATCATGTATGTAGCCGTTCAGCAGCCGCGGCTTGATGTAACGGAAACC
GGGGCAGCGCCGGTGTCGCAAATTTTTACGACGGTGATGAAAAGCAGTTTGCAATATTTGCATATCGAGCCGGTAGAGAA
AAAACAGACGGAAACGAAGAAGGCGGAACGGCGCGCGCTTGATTCGCTGATCGGTCAAACTGCCGTCTCAGCGGCGGAAC
AGCTGAAAGAAAAAGGATATGTGCCGGTTGTCATCGGCAACGGTAAAAATGTTGAACGGCAGCTTCCGAGCGGTGGGGAG
CGCGTTCTTCCAGGGGAGCGAGTCGTGCTGAAAACGGACGGATCAGCAACGATGCCGGATTTGCGCGGCTTTTCGCTGCG
GGACGCCATGAAAGTGGCCAACGTGCTCGAGTTGCGTCCGAGTACGAAAGGCACCGGTTATGTCGTCAGCCAAAGCATTC
GTCCGGGAGCGGAAGTGCGCAAAGGAGATTATTTGATTGTCGAGCTGGCTTCTCCACGCCAATGGGAAGAAACGATGGCG
AAGAAAGAAAAAGAGGCGGCTGAACGGAAGGACAACAAGCCGCACGAATGA

Upstream 100 bases:

>100_bases
GCAGCAGTTGAGCTCGTACGAGCGCATTTTGCAAAAGGCGAAGGAGCTTGGGCTGTCACTGAACGAAGACCATGTGAAAG
TTGTAAAGGAATGATGGAGA

Downstream 100 bases:

>100_bases
TTTTTGCCGTTCTATAACGGGGCGTACAAGCATATAGTGGAACGAGCCCATCACGAAGGAGGTTCCGCCATATGCGCGTG
TCGTACGTTACCGTGCGCAA

Product: high molecular weight penicillin binding protein

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MEMKKHSNTHRGAGILFIVFSLLFFLLFARFIQLQWTGKADGHVLAAKAEQQHKQKRVIEAKRGTIFDRNGTILAQDVPS
YTVVAILDPEMTTNPERPRHVVDPETTAKKLAPLLGMDVKDMERILTKKAKQVEFGSYGRNISFELKRKIEALDLPGIAF
IRDTNRFYPNGTFASHVIGYAQKNAENETVGKMGVEKWLDRYLRETDGYVSFAGDVNGFRLPEAKERIVKPDNGANVYLT
IDGKIQTFLEDAMNNVEKQYKPKKMIGIVADPKTGKILAMATRPSFDPNKRDITNFLNDAISYPYEPGSTMKVFTLAAAI
NEGVYNGKETFRSGAYKVGPNTVRDHNDVGWGTITFDEGVQRSSNVAFSILVKEKLGEDRFLQYLHRFRFDQKTGIDLPN
ESVGQIRYRYPIERITTGFGQGTSVTPIQQIQAATAIANDGKMMKPYIIEKVVDPDNGKVVLDHEPTVAGEPITAETARQ
VREILETVVSSEHGTGRPYQIDGYRVAGKTGTAQIPSAAGGYMTGRENYIFSFLGMAPADDPRLIMYVAVQQPRLDVTET
GAAPVSQIFTTVMKSSLQYLHIEPVEKKQTETKKAERRALDSLIGQTAVSAAEQLKEKGYVPVVIGNGKNVERQLPSGGE
RVLPGERVVLKTDGSATMPDLRGFSLRDAMKVANVLELRPSTKGTGYVVSQSIRPGAEVRKGDYLIVELASPRQWEETMA
KKEKEAAERKDNKPHE

Sequences:

>Translated_736_residues
MEMKKHSNTHRGAGILFIVFSLLFFLLFARFIQLQWTGKADGHVLAAKAEQQHKQKRVIEAKRGTIFDRNGTILAQDVPS
YTVVAILDPEMTTNPERPRHVVDPETTAKKLAPLLGMDVKDMERILTKKAKQVEFGSYGRNISFELKRKIEALDLPGIAF
IRDTNRFYPNGTFASHVIGYAQKNAENETVGKMGVEKWLDRYLRETDGYVSFAGDVNGFRLPEAKERIVKPDNGANVYLT
IDGKIQTFLEDAMNNVEKQYKPKKMIGIVADPKTGKILAMATRPSFDPNKRDITNFLNDAISYPYEPGSTMKVFTLAAAI
NEGVYNGKETFRSGAYKVGPNTVRDHNDVGWGTITFDEGVQRSSNVAFSILVKEKLGEDRFLQYLHRFRFDQKTGIDLPN
ESVGQIRYRYPIERITTGFGQGTSVTPIQQIQAATAIANDGKMMKPYIIEKVVDPDNGKVVLDHEPTVAGEPITAETARQ
VREILETVVSSEHGTGRPYQIDGYRVAGKTGTAQIPSAAGGYMTGRENYIFSFLGMAPADDPRLIMYVAVQQPRLDVTET
GAAPVSQIFTTVMKSSLQYLHIEPVEKKQTETKKAERRALDSLIGQTAVSAAEQLKEKGYVPVVIGNGKNVERQLPSGGE
RVLPGERVVLKTDGSATMPDLRGFSLRDAMKVANVLELRPSTKGTGYVVSQSIRPGAEVRKGDYLIVELASPRQWEETMA
KKEKEAAERKDNKPHE
>Mature_736_residues
MEMKKHSNTHRGAGILFIVFSLLFFLLFARFIQLQWTGKADGHVLAAKAEQQHKQKRVIEAKRGTIFDRNGTILAQDVPS
YTVVAILDPEMTTNPERPRHVVDPETTAKKLAPLLGMDVKDMERILTKKAKQVEFGSYGRNISFELKRKIEALDLPGIAF
IRDTNRFYPNGTFASHVIGYAQKNAENETVGKMGVEKWLDRYLRETDGYVSFAGDVNGFRLPEAKERIVKPDNGANVYLT
IDGKIQTFLEDAMNNVEKQYKPKKMIGIVADPKTGKILAMATRPSFDPNKRDITNFLNDAISYPYEPGSTMKVFTLAAAI
NEGVYNGKETFRSGAYKVGPNTVRDHNDVGWGTITFDEGVQRSSNVAFSILVKEKLGEDRFLQYLHRFRFDQKTGIDLPN
ESVGQIRYRYPIERITTGFGQGTSVTPIQQIQAATAIANDGKMMKPYIIEKVVDPDNGKVVLDHEPTVAGEPITAETARQ
VREILETVVSSEHGTGRPYQIDGYRVAGKTGTAQIPSAAGGYMTGRENYIFSFLGMAPADDPRLIMYVAVQQPRLDVTET
GAAPVSQIFTTVMKSSLQYLHIEPVEKKQTETKKAERRALDSLIGQTAVSAAEQLKEKGYVPVVIGNGKNVERQLPSGGE
RVLPGERVVLKTDGSATMPDLRGFSLRDAMKVANVLELRPSTKGTGYVVSQSIRPGAEVRKGDYLIVELASPRQWEETMA
KKEKEAAERKDNKPHE

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=597, Percent_Identity=27.4706867671692, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1786854, Length=559, Percent_Identity=25.9391771019678, Blast_Score=146, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 81903; Mature: 81903

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMKKHSNTHRGAGILFIVFSLLFFLLFARFIQLQWTGKADGHVLAAKAEQQHKQKRVIE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEECHHHHHHHHHHHH
AKRGTIFDRNGTILAQDVPSYTVVAILDPEMTTNPERPRHVVDPETTAKKLAPLLGMDVK
HHCCEEEECCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHH
DMERILTKKAKQVEFGSYGRNISFELKRKIEALDLPGIAFIRDTNRFYPNGTFASHVIGY
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHH
AQKNAENETVGKMGVEKWLDRYLRETDGYVSFAGDVNGFRLPEAKERIVKPDNGANVYLT
HHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCCCCCCCEEEEE
IDGKIQTFLEDAMNNVEKQYKPKKMIGIVADPKTGKILAMATRPSFDPNKRDITNFLNDA
ECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHH
ISYPYEPGSTMKVFTLAAAINEGVYNGKETFRSGAYKVGPNTVRDHNDVGWGTITFDEGV
HCCCCCCCCCEEHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCCCCCCCCCEEEEECCCC
QRSSNVAFSILVKEKLGEDRFLQYLHRFRFDQKTGIDLPNESVGQIRYRYPIERITTGFG
CCCCCCEEEEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCC
QGTSVTPIQQIQAATAIANDGKMMKPYIIEKVVDPDNGKVVLDHEPTVAGEPITAETARQ
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHH
VREILETVVSSEHGTGRPYQIDGYRVAGKTGTAQIPSAAGGYMTGRENYIFSFLGMAPAD
HHHHHHHHHCCCCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
DPRLIMYVAVQQPRLDVTETGAAPVSQIFTTVMKSSLQYLHIEPVEKKQTETKKAERRAL
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
DSLIGQTAVSAAEQLKEKGYVPVVIGNGKNVERQLPSGGERVLPGERVVLKTDGSATMPD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCCCC
LRGFSLRDAMKVANVLELRPSTKGTGYVVSQSIRPGAEVRKGDYLIVELASPRQWEETMA
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHH
KKEKEAAERKDNKPHE
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEMKKHSNTHRGAGILFIVFSLLFFLLFARFIQLQWTGKADGHVLAAKAEQQHKQKRVIE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEECHHHHHHHHHHHH
AKRGTIFDRNGTILAQDVPSYTVVAILDPEMTTNPERPRHVVDPETTAKKLAPLLGMDVK
HHCCEEEECCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHH
DMERILTKKAKQVEFGSYGRNISFELKRKIEALDLPGIAFIRDTNRFYPNGTFASHVIGY
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHH
AQKNAENETVGKMGVEKWLDRYLRETDGYVSFAGDVNGFRLPEAKERIVKPDNGANVYLT
HHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHCCCCCCCCEEEEE
IDGKIQTFLEDAMNNVEKQYKPKKMIGIVADPKTGKILAMATRPSFDPNKRDITNFLNDA
ECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHH
ISYPYEPGSTMKVFTLAAAINEGVYNGKETFRSGAYKVGPNTVRDHNDVGWGTITFDEGV
HCCCCCCCCCEEHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCCCCCCCCCEEEEECCCC
QRSSNVAFSILVKEKLGEDRFLQYLHRFRFDQKTGIDLPNESVGQIRYRYPIERITTGFG
CCCCCCEEEEEEHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCC
QGTSVTPIQQIQAATAIANDGKMMKPYIIEKVVDPDNGKVVLDHEPTVAGEPITAETARQ
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHH
VREILETVVSSEHGTGRPYQIDGYRVAGKTGTAQIPSAAGGYMTGRENYIFSFLGMAPAD
HHHHHHHHHCCCCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
DPRLIMYVAVQQPRLDVTETGAAPVSQIFTTVMKSSLQYLHIEPVEKKQTETKKAERRAL
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
DSLIGQTAVSAAEQLKEKGYVPVVIGNGKNVERQLPSGGERVLPGERVVLKTDGSATMPD
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCCCC
LRGFSLRDAMKVANVLELRPSTKGTGYVVSQSIRPGAEVRKGDYLIVELASPRQWEETMA
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHH
KKEKEAAERKDNKPHE
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]