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The map label for this gene is pleD [H]

Identifier: 138894571

GI number: 138894571

Start: 977054

End: 978235

Strand: Direct

Name: pleD [H]

Synonym: GTNG_0901

Alternate gene names: 138894571

Gene position: 977054-978235 (Clockwise)

Preceding gene: 138894570

Following gene: 138894574

Centisome position: 27.52

GC content: 52.54

Gene sequence:

>1182_bases
TTGGATTCCTATATCGTCTCGTTACTAGACCGGATGAAAGAGCAGTATGAACAATGGGAGTCTTCATCTTCGCTCGACGG
GGCGGAGCTGACTCAGTTTTTCGATGCCATCGGCCGAGCGGCGAAGGTGACCGGCCGCGATGATCTTGCTGCTGAAGTAG
AACGGATTCTCCGCCGGATGAACAGGCAAAAGGAACGGCAATGGACGGTGGAGGAGGTGCTAGCGGAGATGTTTCCACTC
CTGCGCCGCTGCTATGAAGCCGAGGAAACGGATTCTACAATCGCTTCCTTCTTGCAACAACAAGGGGCGGAAGCTGACAT
TCTTCTCTGCGGAAACGACCCGTTATTTTTTGCTTATATCCGTCGATCATGGCAAACCGTACCTTGGCGCTTCATCACGC
TTCCTTCCCTCGAACAAGCAGCCGCATCCATGTTTCGCCTCAACCCGGACTGCATCATCGTCAACATCGGGGAAGACGAT
TTAGAAACTCCTCCTTTCGTTGCTCTGCTGAAGACCGCCAGTCAGCCTTCCTACATTCCAGTAGTGATCATTAGCAGAGA
CGGACGAAAAGCTGTACATCTGAAATGTTATGGGCTTGGCGCCGACGATGTCATCGCGCAGCCGGTGGCAGCTAGTGAGC
TATACATTCGTGTTCACCGTCTCGTAGAAAAAAAGCGGAAAATTGATAAATTGGTGCTTATTGATGAGTTAACCGGCGTC
TATAACCGTAAATATTTACCGCGAGTGTATGTCCGCCTGCGCAGCGATCTTGAGCGTTTCGGGACACCGAGCTGTTTGGC
ACTGCTTGATTTGGACCATTTTAAGCAAGTCAACGACCGCTTCGGACACTTAGTGGGGGATGCGGTGTTAAAAAAACTGG
CCGCCTTTTTGCTTCAACATACGCGCGGAATGGACACGGTCATTCGTTTCGGGGGTGAAGAGTTTATCGTTTGGCTGGCA
AAAACCTCGACATGCGAGGCGCACCGTGTGCTTGGGCGGCTGCAGCGTCAGTTCGCCTCAGAGGAAATCGAAGCAGCTGG
CGTACGCCTATCGTGCACGTTTTCCGCCGGTTTTGTCGAGTGCTCCGACCCGAACGAATCGCTTGATCATTGGCTCAAAC
TGGCCGATAAAGCGCTATATGCAGCCAAAAAGAGCGGCGGCAGCCGGGTTGAGGGAGTTTAG

Upstream 100 bases:

>100_bases
TTAATAAATATATAGGAAAAGATGAGTACGGAAATGAAAGCAGTTCGAGGGCTTTTTATCGGGATTACGGAATGGCTAAC
ATAAAGAAAGGAGAGGGAAG

Downstream 100 bases:

>100_bases
AAGCAAGCCACTTCCGTCAATGGCAGTCCGGCGGAGGAACAGCATCGCCGAGCGGGCAAACAGAGATAGTCTGTTGCCTT
TGTCGACGGCGGTGCACTGG

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MDSYIVSLLDRMKEQYEQWESSSSLDGAELTQFFDAIGRAAKVTGRDDLAAEVERILRRMNRQKERQWTVEEVLAEMFPL
LRRCYEAEETDSTIASFLQQQGAEADILLCGNDPLFFAYIRRSWQTVPWRFITLPSLEQAAASMFRLNPDCIIVNIGEDD
LETPPFVALLKTASQPSYIPVVIISRDGRKAVHLKCYGLGADDVIAQPVAASELYIRVHRLVEKKRKIDKLVLIDELTGV
YNRKYLPRVYVRLRSDLERFGTPSCLALLDLDHFKQVNDRFGHLVGDAVLKKLAAFLLQHTRGMDTVIRFGGEEFIVWLA
KTSTCEAHRVLGRLQRQFASEEIEAAGVRLSCTFSAGFVECSDPNESLDHWLKLADKALYAAKKSGGSRVEGV

Sequences:

>Translated_393_residues
MDSYIVSLLDRMKEQYEQWESSSSLDGAELTQFFDAIGRAAKVTGRDDLAAEVERILRRMNRQKERQWTVEEVLAEMFPL
LRRCYEAEETDSTIASFLQQQGAEADILLCGNDPLFFAYIRRSWQTVPWRFITLPSLEQAAASMFRLNPDCIIVNIGEDD
LETPPFVALLKTASQPSYIPVVIISRDGRKAVHLKCYGLGADDVIAQPVAASELYIRVHRLVEKKRKIDKLVLIDELTGV
YNRKYLPRVYVRLRSDLERFGTPSCLALLDLDHFKQVNDRFGHLVGDAVLKKLAAFLLQHTRGMDTVIRFGGEEFIVWLA
KTSTCEAHRVLGRLQRQFASEEIEAAGVRLSCTFSAGFVECSDPNESLDHWLKLADKALYAAKKSGGSRVEGV
>Mature_393_residues
MDSYIVSLLDRMKEQYEQWESSSSLDGAELTQFFDAIGRAAKVTGRDDLAAEVERILRRMNRQKERQWTVEEVLAEMFPL
LRRCYEAEETDSTIASFLQQQGAEADILLCGNDPLFFAYIRRSWQTVPWRFITLPSLEQAAASMFRLNPDCIIVNIGEDD
LETPPFVALLKTASQPSYIPVVIISRDGRKAVHLKCYGLGADDVIAQPVAASELYIRVHRLVEKKRKIDKLVLIDELTGV
YNRKYLPRVYVRLRSDLERFGTPSCLALLDLDHFKQVNDRFGHLVGDAVLKKLAAFLLQHTRGMDTVIRFGGEEFIVWLA
KTSTCEAHRVLGRLQRQFASEEIEAAGVRLSCTFSAGFVECSDPNESLDHWLKLADKALYAAKKSGGSRVEGV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=163, Percent_Identity=34.9693251533742, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=32.2033898305085, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=37.1794871794872, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI87082007, Length=159, Percent_Identity=34.5911949685535, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787262, Length=159, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081881, Length=176, Percent_Identity=33.5227272727273, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87081974, Length=160, Percent_Identity=33.75, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788381, Length=156, Percent_Identity=31.4102564102564, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=174, Percent_Identity=29.8850574712644, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787541, Length=175, Percent_Identity=30.8571428571429, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788085, Length=189, Percent_Identity=27.5132275132275, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44515; Mature: 44515

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSYIVSLLDRMKEQYEQWESSSSLDGAELTQFFDAIGRAAKVTGRDDLAAEVERILRRM
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHEECCCCHHHHHHHHHHHHHH
NRQKERQWTVEEVLAEMFPLLRRCYEAEETDSTIASFLQQQGAEADILLCGNDPLFFAYI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEHHHH
RRSWQTVPWRFITLPSLEQAAASMFRLNPDCIIVNIGEDDLETPPFVALLKTASQPSYIP
HHCCCCCCEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEEE
VVIISRDGRKAVHLKCYGLGADDVIAQPVAASELYIRVHRLVEKKRKIDKLVLIDELTGV
EEEEECCCCEEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNRKYLPRVYVRLRSDLERFGTPSCLALLDLDHFKQVNDRFGHLVGDAVLKKLAAFLLQH
HCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRGMDTVIRFGGEEFIVWLAKTSTCEAHRVLGRLQRQFASEEIEAAGVRLSCTFSAGFVE
HCCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCEEE
CSDPNESLDHWLKLADKALYAAKKSGGSRVEGV
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDSYIVSLLDRMKEQYEQWESSSSLDGAELTQFFDAIGRAAKVTGRDDLAAEVERILRRM
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHEECCCCHHHHHHHHHHHHHH
NRQKERQWTVEEVLAEMFPLLRRCYEAEETDSTIASFLQQQGAEADILLCGNDPLFFAYI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEHHHH
RRSWQTVPWRFITLPSLEQAAASMFRLNPDCIIVNIGEDDLETPPFVALLKTASQPSYIP
HHCCCCCCEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCEEE
VVIISRDGRKAVHLKCYGLGADDVIAQPVAASELYIRVHRLVEKKRKIDKLVLIDELTGV
EEEEECCCCEEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNRKYLPRVYVRLRSDLERFGTPSCLALLDLDHFKQVNDRFGHLVGDAVLKKLAAFLLQH
HCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRGMDTVIRFGGEEFIVWLAKTSTCEAHRVLGRLQRQFASEEIEAAGVRLSCTFSAGFVE
HCCHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCEEE
CSDPNESLDHWLKLADKALYAAKKSGGSRVEGV
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA