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The map label for this gene is ptsI [H]

Identifier: 138894550

GI number: 138894550

Start: 953731

End: 955452

Strand: Direct

Name: ptsI [H]

Synonym: GTNG_0880

Alternate gene names: 138894550

Gene position: 953731-955452 (Clockwise)

Preceding gene: 138894549

Following gene: 138894554

Centisome position: 26.86

GC content: 52.26

Gene sequence:

>1722_bases
ATGGAAAAAACCATCCATGGAATTGCTGCATCGAGCGGTATCGCCATTGCCAAGGCATACCGCTTAGAAACCCCTCATTT
GGCGGCTGAAAAACAGCCGATCACTGACGCTGAGGCGGAAGTGGCGCGGCTCGAGGCGGCGGTCGCCAAGGCGAAAGAAG
AGCTTGAGATGATTAAACAGCACGCCTTAGAGAAGCTCGGTGAAGACAAAGCCGCCATTTTTGCTGCCCATTTGCTTGTG
CTTGATGATCCGGAACTGTTAAACCCGATTAAAGAAAAAATTAAAACGGAACAAGTGAATGCCGAGTATGCGCTTGACGA
GACCGCATCGTTTTTCATTTCCATGTTTGAAGGCATGGATAATGAATATATGAAAGAGAGAGCTGCTGACATCCGCGATG
TAACGAAGCGCGTTCTCGCCCATTTGCTCGGCGTTGCCATCTCCAACCCAAGCCTGATTTCCGAAGAGGTCGTCATCATT
GCTGAAGATTTGACACCATCCGATACGGCGCAGCTGAATCGACAGTATGTCAAAGGATTTGCGACCGACATCGGTGGACG
GACATCGCACTCGGCCATTATGGCCCGCTCGCTCGAAATTCCGGCTGTCGTCGGCACGAAGACGGTAACAGCGGAAGTGA
AAAACGGCGACATTGTCGTCATCGACGGGCTTGATGGACAAGTCGTTGTCAATCCGTCGCCGGAGCTGCTCGCTCATTAT
GAAGAAAAGCAAGCCCGCTATGAAGCGCAAAAAGCGGAATGGGCAAAACTCGTTCATGAGCCGACAGTGACGGCTGACGG
TGTACATGTCGAGCTGGCAGCCAACATTGGCACACCGGACGATGTAAAAGGGGTGCTTGCTAACGGGGCGGAAGGCATCG
GTTTGTACCGGACGGAGTTTTTATATATGGGCCGCCCGGAACTGCCGACAGAGGAAGAGCAGTTCGTCGCCTACAAAACG
GTGCTTGAGCAAATGAAGGGCAAGCCGGTCGTCGTCCGCACGCTTGACATCGGCGGTGATAAAGAGCTGCCATATTTAAA
CTTGCCGAAAGAAATGAACCCGTTTTTAGGGTTCCGTGCGATCCGTCTTTGCTTGGAAATGCAGGAGATGTTCCGCACTC
AGCTGCGTGCCCTGCTGCGGGCGAGCGTGCATGGCAATTTGAAAATCATGTTTCCGATGATTGCCACGCTCGACGAATTC
CGTCAGGCGAAAGCGATTTTGCTCGAAGAAAAAGAGGCGCTCCTGCGTCAAGGCACGCCAGTCGCCGATGACATTGAAGT
CGGCATGATGGTCGAGATTCCGGCTGCTGCCGTTATGGCGGATCAGTTTGCAAAAGAAGTGGATTTCTTCAGCATTGGCA
CGAACGACTTAATCCAATATACGATGGCCGCCGACCGAATGAATGAGCGTGTCTCGTATTTGTATCAACCGTACAATCCG
GCCATTTTGCGGCTTATCAGCCATGTGATTGATGCTGCCCACCGCGAAGGAAAATGGGTCGGTATGTGCGGGGAAATGGC
TGGCGACCCGATTGCAATTCCGATTTTGCTTGCCCTCGGACTTGACGAGTTCAGCATGAGCGCCACCTCGATTTTACCGG
CGCGCGCTCAGCTGAAACAGCTGTCAAAAGAAGAGGCAACTCGCATCAAAGAGAAGGTTCTGTCGCTCGGAACAGCCGAG
GACGTTGTGGCCTTTGTCAAACAAACATTTTCTCTTGCTTAA

Upstream 100 bases:

>100_bases
CGAAAGGGGCAACGATTAAAATTACGGCAGAAGGGGCCGATGCAGCAGAGGCGATGGCAGCGTTAACGGATACGTTGGCG
AAAGAAGGCCTTGCTCAGTA

Downstream 100 bases:

>100_bases
ATGAAGGCGTGGCAACGTTTGTGTTGCCGCGCTTTTTCGTGTTGCTTTATGCCAACAACGGGGCAGATGTTTCGTAGCCA
TAGCGTTGCCTGATGGTGAG

Product: phosphotransferase system enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MEKTIHGIAASSGIAIAKAYRLETPHLAAEKQPITDAEAEVARLEAAVAKAKEELEMIKQHALEKLGEDKAAIFAAHLLV
LDDPELLNPIKEKIKTEQVNAEYALDETASFFISMFEGMDNEYMKERAADIRDVTKRVLAHLLGVAISNPSLISEEVVII
AEDLTPSDTAQLNRQYVKGFATDIGGRTSHSAIMARSLEIPAVVGTKTVTAEVKNGDIVVIDGLDGQVVVNPSPELLAHY
EEKQARYEAQKAEWAKLVHEPTVTADGVHVELAANIGTPDDVKGVLANGAEGIGLYRTEFLYMGRPELPTEEEQFVAYKT
VLEQMKGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEMQEMFRTQLRALLRASVHGNLKIMFPMIATLDEF
RQAKAILLEEKEALLRQGTPVADDIEVGMMVEIPAAAVMADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
AILRLISHVIDAAHREGKWVGMCGEMAGDPIAIPILLALGLDEFSMSATSILPARAQLKQLSKEEATRIKEKVLSLGTAE
DVVAFVKQTFSLA

Sequences:

>Translated_573_residues
MEKTIHGIAASSGIAIAKAYRLETPHLAAEKQPITDAEAEVARLEAAVAKAKEELEMIKQHALEKLGEDKAAIFAAHLLV
LDDPELLNPIKEKIKTEQVNAEYALDETASFFISMFEGMDNEYMKERAADIRDVTKRVLAHLLGVAISNPSLISEEVVII
AEDLTPSDTAQLNRQYVKGFATDIGGRTSHSAIMARSLEIPAVVGTKTVTAEVKNGDIVVIDGLDGQVVVNPSPELLAHY
EEKQARYEAQKAEWAKLVHEPTVTADGVHVELAANIGTPDDVKGVLANGAEGIGLYRTEFLYMGRPELPTEEEQFVAYKT
VLEQMKGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEMQEMFRTQLRALLRASVHGNLKIMFPMIATLDEF
RQAKAILLEEKEALLRQGTPVADDIEVGMMVEIPAAAVMADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
AILRLISHVIDAAHREGKWVGMCGEMAGDPIAIPILLALGLDEFSMSATSILPARAQLKQLSKEEATRIKEKVLSLGTAE
DVVAFVKQTFSLA
>Mature_573_residues
MEKTIHGIAASSGIAIAKAYRLETPHLAAEKQPITDAEAEVARLEAAVAKAKEELEMIKQHALEKLGEDKAAIFAAHLLV
LDDPELLNPIKEKIKTEQVNAEYALDETASFFISMFEGMDNEYMKERAADIRDVTKRVLAHLLGVAISNPSLISEEVVII
AEDLTPSDTAQLNRQYVKGFATDIGGRTSHSAIMARSLEIPAVVGTKTVTAEVKNGDIVVIDGLDGQVVVNPSPELLAHY
EEKQARYEAQKAEWAKLVHEPTVTADGVHVELAANIGTPDDVKGVLANGAEGIGLYRTEFLYMGRPELPTEEEQFVAYKT
VLEQMKGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRAIRLCLEMQEMFRTQLRALLRASVHGNLKIMFPMIATLDEF
RQAKAILLEEKEALLRQGTPVADDIEVGMMVEIPAAAVMADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
AILRLISHVIDAAHREGKWVGMCGEMAGDPIAIPILLALGLDEFSMSATSILPARAQLKQLSKEEATRIKEKVLSLGTAE
DVVAFVKQTFSLA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=566, Percent_Identity=50.8833922261484, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1788726, Length=564, Percent_Identity=36.5248226950355, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI48994992, Length=500, Percent_Identity=39, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789193, Length=575, Percent_Identity=33.5652173913043, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1787994, Length=433, Percent_Identity=29.3302540415704, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI226510935, Length=133, Percent_Identity=36.8421052631579, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63037; Mature: 63037

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTIHGIAASSGIAIAKAYRLETPHLAAEKQPITDAEAEVARLEAAVAKAKEELEMIKQ
CCCCCCCHHCCCCCEEHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HALEKLGEDKAAIFAAHLLVLDDPELLNPIKEKIKTEQVNAEYALDETASFFISMFEGMD
HHHHHHCCCHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
NEYMKERAADIRDVTKRVLAHLLGVAISNPSLISEEVVIIAEDLTPSDTAQLNRQYVKGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
ATDIGGRTSHSAIMARSLEIPAVVGTKTVTAEVKNGDIVVIDGLDGQVVVNPSPELLAHY
HHHCCCCCCHHHHHHHCCCCCEEECCCEEEEEECCCCEEEEECCCCEEEECCCHHHHHHH
EEKQARYEAQKAEWAKLVHEPTVTADGVHVELAANIGTPDDVKGVLANGAEGIGLYRTEF
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEE
LYMGRPELPTEEEQFVAYKTVLEQMKGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRA
EEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCHHCCHHHHHHH
IRLCLEMQEMFRTQLRALLRASVHGNLKIMFPMIATLDEFRQAKAILLEEKEALLRQGTP
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VADDIEVGMMVEIPAAAVMADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
CCCCCCCCEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCH
AILRLISHVIDAAHREGKWVGMCGEMAGDPIAIPILLALGLDEFSMSATSILPARAQLKQ
HHHHHHHHHHHHHHHCCCEEEECHHHCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHH
LSKEEATRIKEKVLSLGTAEDVVAFVKQTFSLA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKTIHGIAASSGIAIAKAYRLETPHLAAEKQPITDAEAEVARLEAAVAKAKEELEMIKQ
CCCCCCCHHCCCCCEEHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HALEKLGEDKAAIFAAHLLVLDDPELLNPIKEKIKTEQVNAEYALDETASFFISMFEGMD
HHHHHHCCCHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
NEYMKERAADIRDVTKRVLAHLLGVAISNPSLISEEVVIIAEDLTPSDTAQLNRQYVKGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
ATDIGGRTSHSAIMARSLEIPAVVGTKTVTAEVKNGDIVVIDGLDGQVVVNPSPELLAHY
HHHCCCCCCHHHHHHHCCCCCEEECCCEEEEEECCCCEEEEECCCCEEEECCCHHHHHHH
EEKQARYEAQKAEWAKLVHEPTVTADGVHVELAANIGTPDDVKGVLANGAEGIGLYRTEF
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEE
LYMGRPELPTEEEQFVAYKTVLEQMKGKPVVVRTLDIGGDKELPYLNLPKEMNPFLGFRA
EEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEECCCHHCCHHHHHHH
IRLCLEMQEMFRTQLRALLRASVHGNLKIMFPMIATLDEFRQAKAILLEEKEALLRQGTP
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VADDIEVGMMVEIPAAAVMADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNP
CCCCCCCCEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCH
AILRLISHVIDAAHREGKWVGMCGEMAGDPIAIPILLALGLDEFSMSATSILPARAQLKQ
HHHHHHHHHHHHHHHCCCEEEECHHHCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHH
LSKEEATRIKEKVLSLGTAEDVVAFVKQTFSLA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA