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The map label for this gene is hsdS [H]

Identifier: 138894435

GI number: 138894435

Start: 839388

End: 840917

Strand: Direct

Name: hsdS [H]

Synonym: GTNG_0763

Alternate gene names: 138894435

Gene position: 839388-840917 (Clockwise)

Preceding gene: 138894434

Following gene: 138894437

Centisome position: 23.64

GC content: 33.66

Gene sequence:

>1530_bases
ATGAGCAAAAAGAAACAAAAGTCTATCGAAGAACTACTCGAAGAAGCGTTAGTACCTGAAGAAGAGCAACCGTATATAGT
TCCAAAAAATTGGGTTTGGACTAGAACTGGAATTACACATGAAATAGTAACTGGATCTACTCCATCTAAAAAAAATAATG
AGTATTATGGTGGAAACTTTCCATTTGTTAAACCCGGAGATTTAGATCAGAAAGATTCGGTGACAGTAGCTTCTGAATAT
CTTACTGATAAAGGTAAAGAAGTAAGTAGAGTAATTCCAAAACATTCAACCTTGGTTTGTTGTATTGGTTCGATAGGAAA
GGTTGGTTTTAATTTAGTAGAATGTACTACAAATCAACAAATAAATTCTCTAATTCCAAATAAAAAAGTAATTTATCCCA
AATACACATACTATTTTTCATTAAGTTCTGTTTACCAAAATTTATTATCAAAATCATCTTCATCTACAACTGTGTCCATA
ATAAACAAAAGTAAAATGTCTAAGCTTCCATTTGCACTTCCACCTTTAAACGAACAAAAACACATTGCTGAAAAAGTCGA
TCGGCTTTTTGCTAAAATTGATGAAGCGAAGCGGTTGATTGAAGAAGTGAAAGAGTCGTTTGAACTTCGACGGGCAGCGA
TTTTGGATAAGGCGTTTCGAGGGGAACTAACACGATCATGGCGTAAAAAGAATGAACATTTAGTTTCTGCTTCCCTTATG
TTGCAAGAAATTGCTTCAGAAAGAAAAAGAAAATATTCAGATTTATGTAGATTAGCTAAAATAAATGGAGAAAAGAAACC
AAGAAAGCTTTATCTAGATGAGGTGCCTGTTATAGAAGAAAAACCTAGACACTCTTTACCAGATACTTGGACGATTACTA
ATATTGGCTTTTTAGCTCATGTGACAAAGTTAGCAGGATTTGAGTATACAAAGTATTTTAACTTAACAGAAACTGGGGAT
GTTCCTGTAATAAGGGCGCAAAATGTTCAGATGGGAGAGTTTATAGAATCTAATATCAAATATATAACTAAAGAAGTATC
TGACTTACTCGAGAGATCACAAGTGCATGGCGGAGAAGTTTTAATGGTATTTATAGGTGCAGGAACAGGAAATGTTTGTA
TGGCACCTAGAGATAACAGACGCTGGCATTTAGCTCCTAATGTAGCAAAGATAACTGTCGATGAAATACTGGCTGAGTAT
CTCAATTTGTATTTACAATCACCAATCGGGCAGTCTTATATAAAGTCAAAAATGAAAGCAACCGCTCAACAAAGTTTGTC
TATGGAAACAATTAGAGATGTATTAGTTTATGTTCCACCGTTGGAGGAACAATATGAAATAGTAAGAATAGTTGAAAGAC
TTCTTGATAATTTGAAAAATGAATATTTAATTTTGAATGATATACACATGAAGATAGACAATATAAAACAATCTATTCTC
TCCAAAGCCTTCCGCGGCGAGCTTGGTACAAACGATCCAACCGATGAACATGCTATTGAGTTGTTAAAAGAAGTACTAAA
GTATAAATAA

Upstream 100 bases:

>100_bases
AAGTCATTGCCGAGTTAAAAGCGGTTGAACAACCTAATAGCTACCAAGCGACTTCTAAAGAAATTCTGAAAGTGGCAGAA
ACATCTGAGGTGCTTAAAAA

Downstream 100 bases:

>100_bases
ACCATTTATGTAAGAGCTCACTAAAATAGAGTGAGCTCTATTTTATCTCCTTCATATTGAAAAAATAGTTGTAAATATAG
ATCTATACTCATTCAACGTT

Product: putative type I specificity subunit HsdS

Products: NA

Alternate protein names: S.StySJI; Type I restriction enzyme StySJI specificity protein; S protein [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSKKKQKSIEELLEEALVPEEEQPYIVPKNWVWTRTGITHEIVTGSTPSKKNNEYYGGNFPFVKPGDLDQKDSVTVASEY
LTDKGKEVSRVIPKHSTLVCCIGSIGKVGFNLVECTTNQQINSLIPNKKVIYPKYTYYFSLSSVYQNLLSKSSSSTTVSI
INKSKMSKLPFALPPLNEQKHIAEKVDRLFAKIDEAKRLIEEVKESFELRRAAILDKAFRGELTRSWRKKNEHLVSASLM
LQEIASERKRKYSDLCRLAKINGEKKPRKLYLDEVPVIEEKPRHSLPDTWTITNIGFLAHVTKLAGFEYTKYFNLTETGD
VPVIRAQNVQMGEFIESNIKYITKEVSDLLERSQVHGGEVLMVFIGAGTGNVCMAPRDNRRWHLAPNVAKITVDEILAEY
LNLYLQSPIGQSYIKSKMKATAQQSLSMETIRDVLVYVPPLEEQYEIVRIVERLLDNLKNEYLILNDIHMKIDNIKQSIL
SKAFRGELGTNDPTDEHAIELLKEVLKYK

Sequences:

>Translated_509_residues
MSKKKQKSIEELLEEALVPEEEQPYIVPKNWVWTRTGITHEIVTGSTPSKKNNEYYGGNFPFVKPGDLDQKDSVTVASEY
LTDKGKEVSRVIPKHSTLVCCIGSIGKVGFNLVECTTNQQINSLIPNKKVIYPKYTYYFSLSSVYQNLLSKSSSSTTVSI
INKSKMSKLPFALPPLNEQKHIAEKVDRLFAKIDEAKRLIEEVKESFELRRAAILDKAFRGELTRSWRKKNEHLVSASLM
LQEIASERKRKYSDLCRLAKINGEKKPRKLYLDEVPVIEEKPRHSLPDTWTITNIGFLAHVTKLAGFEYTKYFNLTETGD
VPVIRAQNVQMGEFIESNIKYITKEVSDLLERSQVHGGEVLMVFIGAGTGNVCMAPRDNRRWHLAPNVAKITVDEILAEY
LNLYLQSPIGQSYIKSKMKATAQQSLSMETIRDVLVYVPPLEEQYEIVRIVERLLDNLKNEYLILNDIHMKIDNIKQSIL
SKAFRGELGTNDPTDEHAIELLKEVLKYK
>Mature_508_residues
SKKKQKSIEELLEEALVPEEEQPYIVPKNWVWTRTGITHEIVTGSTPSKKNNEYYGGNFPFVKPGDLDQKDSVTVASEYL
TDKGKEVSRVIPKHSTLVCCIGSIGKVGFNLVECTTNQQINSLIPNKKVIYPKYTYYFSLSSVYQNLLSKSSSSTTVSII
NKSKMSKLPFALPPLNEQKHIAEKVDRLFAKIDEAKRLIEEVKESFELRRAAILDKAFRGELTRSWRKKNEHLVSASLML
QEIASERKRKYSDLCRLAKINGEKKPRKLYLDEVPVIEEKPRHSLPDTWTITNIGFLAHVTKLAGFEYTKYFNLTETGDV
PVIRAQNVQMGEFIESNIKYITKEVSDLLERSQVHGGEVLMVFIGAGTGNVCMAPRDNRRWHLAPNVAKITVDEILAEYL
NLYLQSPIGQSYIKSKMKATAQQSLSMETIRDVLVYVPPLEEQYEIVRIVERLLDNLKNEYLILNDIHMKIDNIKQSILS
KAFRGELGTNDPTDEHAIELLKEVLKYK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=486, Percent_Identity=24.8971193415638, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 58136; Mature: 58005

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKKQKSIEELLEEALVPEEEQPYIVPKNWVWTRTGITHEIVTGSTPSKKNNEYYGGNF
CCCHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCC
PFVKPGDLDQKDSVTVASEYLTDKGKEVSRVIPKHSTLVCCIGSIGKVGFNLVECTTNQQ
CCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCEEEEEHHHHHHHCCEEEEECCCHH
INSLIPNKKVIYPKYTYYFSLSSVYQNLLSKSSSSTTVSIINKSKMSKLPFALPPLNEQK
HHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCCCCCHH
HIAEKVDRLFAKIDEAKRLIEEVKESFELRRAAILDKAFRGELTRSWRKKNEHLVSASLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQEIASERKRKYSDLCRLAKINGEKKPRKLYLDEVPVIEEKPRHSLPDTWTITNIGFLAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEHHHHHHHH
VTKLAGFEYTKYFNLTETGDVPVIRAQNVQMGEFIESNIKYITKEVSDLLERSQVHGGEV
HHHHHCCCHHEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LMVFIGAGTGNVCMAPRDNRRWHLAPNVAKITVDEILAEYLNLYLQSPIGQSYIKSKMKA
EEEEEECCCCCEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TAQQSLSMETIRDVLVYVPPLEEQYEIVRIVERLLDNLKNEYLILNDIHMKIDNIKQSIL
HHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
SKAFRGELGTNDPTDEHAIELLKEVLKYK
HHHHCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKKKQKSIEELLEEALVPEEEQPYIVPKNWVWTRTGITHEIVTGSTPSKKNNEYYGGNF
CCHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCC
PFVKPGDLDQKDSVTVASEYLTDKGKEVSRVIPKHSTLVCCIGSIGKVGFNLVECTTNQQ
CCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCEEEEEHHHHHHHCCEEEEECCCHH
INSLIPNKKVIYPKYTYYFSLSSVYQNLLSKSSSSTTVSIINKSKMSKLPFALPPLNEQK
HHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCCCCCHH
HIAEKVDRLFAKIDEAKRLIEEVKESFELRRAAILDKAFRGELTRSWRKKNEHLVSASLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQEIASERKRKYSDLCRLAKINGEKKPRKLYLDEVPVIEEKPRHSLPDTWTITNIGFLAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEHHHHHHHH
VTKLAGFEYTKYFNLTETGDVPVIRAQNVQMGEFIESNIKYITKEVSDLLERSQVHGGEV
HHHHHCCCHHEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LMVFIGAGTGNVCMAPRDNRRWHLAPNVAKITVDEILAEYLNLYLQSPIGQSYIKSKMKA
EEEEEECCCCCEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TAQQSLSMETIRDVLVYVPPLEEQYEIVRIVERLLDNLKNEYLILNDIHMKIDNIKQSIL
HHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
SKAFRGELGTNDPTDEHAIELLKEVLKYK
HHHHCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2838725; 11677609; 3025838 [H]