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The map label for this gene is hsdM [H]

Identifier: 138894434

GI number: 138894434

Start: 837910

End: 839391

Strand: Direct

Name: hsdM [H]

Synonym: GTNG_0762

Alternate gene names: 138894434

Gene position: 837910-839391 (Clockwise)

Preceding gene: 138894433

Following gene: 138894435

Centisome position: 23.6

GC content: 40.82

Gene sequence:

>1482_bases
ATGAACAACAGAGAAATCGTCCAAAAGCTATGGAATTTATGTAACGTGCTTCGTGATGACGGCATTACCTATCACCAATA
CGTGACAGAGCTAACGTATTTGCTGTTCCTAAAAATGATGAAAGAAACCGGTCAAGAATATATTATTCCAGAAAAATATC
GCTGGGATTCGCTAGTAGAAAAAGACGGGATTGAACTAAAAGAGCATTATCAACAGTTGTTACTTGATTTAGGAAAAGAG
GAAAATGAATTATTAAAACAAATTTATACAGACGCGACATCGAATATTAGAGAACCAAAGAACCTAGAAAAAATTATTCA
ATCCATCAATAACTTAGATTGGTATAACGCGAAGCAAGAAGGTTTAGGCGATTTGTACGAAGGACTGTTAGAAAAAAACG
CGAGTGAAGTCAAATCAGGAGCGGGGCAATATTTTACGCCTCGCGTCCTCATTGATGTCATTGTAGAACTTGTGAATCCA
CAGCCGGGAGAACGTTGTCACGACCCGGCTGCTGGAACCTTTGGTTTTATGATTGCGGCGGACCGTCATGTTCGGGAGCA
AACGGATGATTATTTTGATTTGTCACAAGAAGAAATTGAGTTCCAAAAATATAAAGCTTTCTCAGGAGTGGAGCTTGTCA
GAGATACGCATCGCTTAGCGGTCATGAATGCGTTGCTTCATGATATTCATGGAGAAATCTTGTTAGGGGATACCCTCTCA
TCACTTGGTGAAAGTTTGAAAAATTATGATGTGATTCTAACGAATCCGCCTTTTGGAACAAAAAAAGGTGGCGAGCGGGC
AACACGAACAGACTTTACCTTTACAACATCAAACAAACAGCTGAATTTCTTGCAACATATTTACAGAGCTTTAAAACCGA
ATGGAAAAGCACGTGCCGCTGTTATTGTACCTGACAACGTATTGTTTGAAGGCGGAGTCGGCGCCGACATTCGTCGAGAC
TTAATGGACAAATGTAATTTGCACACGATTTTACGTTTGCCGACAGGGATTTTCTATGCCCAAGGGGTGAAAACGAACGT
TTTATTCTTCACCCGTGGGAAAACAGATGTTGGCAATACGAAAGAAGTATGGGTGTACGATTTACGGACGAACATGCCTT
CCTTTGGTAAGCGGAATCCGTTGACGAAAGAACATTTTGAAGGATTTATTAAAGCTTACACGGCAGAAGATCGCCGTAAA
GTCAAAGATGAGCGCTGGAATGTTTTTACTCGTGAAGAAATTGCGAAAAACGGCGATAGCTTAGATATTGGCTTAATTGC
CGATGAATCCCTGTCCGTCTACGAAAACTTACCAGACCCCATCGACTCCGCCGAAGAAGCTGTTGAAAAATTAGAATTGG
CGATTTCGTTACTAAACGAAGTCATTGCCGAGTTAAAAGCGGTTGAACAACCTAATAGCTACCAAGCGACTTCTAAAGAA
ATTCTGAAAGTGGCAGAAACATCTGAGGTGCTTAAAAAATGA

Upstream 100 bases:

>100_bases
TAAACAATTAAATAAAATTTTCGACGGACAATTACCTCACATTGTTCGGGAAATTAATTACAACTTATACAACTATCATT
CTAAGAAGGAGCAAGCGTAG

Downstream 100 bases:

>100_bases
GCAAAAAGAAACAAAAGTCTATCGAAGAACTACTCGAAGAAGCGTTAGTACCTGAAGAAGAGCAACCGTATATAGTTCCA
AAAAATTGGGTTTGGACTAG

Product: Type I restriction enzyme StySPI M protein

Products: NA

Alternate protein names: M.EcoKI [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MNNREIVQKLWNLCNVLRDDGITYHQYVTELTYLLFLKMMKETGQEYIIPEKYRWDSLVEKDGIELKEHYQQLLLDLGKE
ENELLKQIYTDATSNIREPKNLEKIIQSINNLDWYNAKQEGLGDLYEGLLEKNASEVKSGAGQYFTPRVLIDVIVELVNP
QPGERCHDPAAGTFGFMIAADRHVREQTDDYFDLSQEEIEFQKYKAFSGVELVRDTHRLAVMNALLHDIHGEILLGDTLS
SLGESLKNYDVILTNPPFGTKKGGERATRTDFTFTTSNKQLNFLQHIYRALKPNGKARAAVIVPDNVLFEGGVGADIRRD
LMDKCNLHTILRLPTGIFYAQGVKTNVLFFTRGKTDVGNTKEVWVYDLRTNMPSFGKRNPLTKEHFEGFIKAYTAEDRRK
VKDERWNVFTREEIAKNGDSLDIGLIADESLSVYENLPDPIDSAEEAVEKLELAISLLNEVIAELKAVEQPNSYQATSKE
ILKVAETSEVLKK

Sequences:

>Translated_493_residues
MNNREIVQKLWNLCNVLRDDGITYHQYVTELTYLLFLKMMKETGQEYIIPEKYRWDSLVEKDGIELKEHYQQLLLDLGKE
ENELLKQIYTDATSNIREPKNLEKIIQSINNLDWYNAKQEGLGDLYEGLLEKNASEVKSGAGQYFTPRVLIDVIVELVNP
QPGERCHDPAAGTFGFMIAADRHVREQTDDYFDLSQEEIEFQKYKAFSGVELVRDTHRLAVMNALLHDIHGEILLGDTLS
SLGESLKNYDVILTNPPFGTKKGGERATRTDFTFTTSNKQLNFLQHIYRALKPNGKARAAVIVPDNVLFEGGVGADIRRD
LMDKCNLHTILRLPTGIFYAQGVKTNVLFFTRGKTDVGNTKEVWVYDLRTNMPSFGKRNPLTKEHFEGFIKAYTAEDRRK
VKDERWNVFTREEIAKNGDSLDIGLIADESLSVYENLPDPIDSAEEAVEKLELAISLLNEVIAELKAVEQPNSYQATSKE
ILKVAETSEVLKK
>Mature_493_residues
MNNREIVQKLWNLCNVLRDDGITYHQYVTELTYLLFLKMMKETGQEYIIPEKYRWDSLVEKDGIELKEHYQQLLLDLGKE
ENELLKQIYTDATSNIREPKNLEKIIQSINNLDWYNAKQEGLGDLYEGLLEKNASEVKSGAGQYFTPRVLIDVIVELVNP
QPGERCHDPAAGTFGFMIAADRHVREQTDDYFDLSQEEIEFQKYKAFSGVELVRDTHRLAVMNALLHDIHGEILLGDTLS
SLGESLKNYDVILTNPPFGTKKGGERATRTDFTFTTSNKQLNFLQHIYRALKPNGKARAAVIVPDNVLFEGGVGADIRRD
LMDKCNLHTILRLPTGIFYAQGVKTNVLFFTRGKTDVGNTKEVWVYDLRTNMPSFGKRNPLTKEHFEGFIKAYTAEDRRK
VKDERWNVFTREEIAKNGDSLDIGLIADESLSVYENLPDPIDSAEEAVEKLELAISLLNEVIAELKAVEQPNSYQATSKE
ILKVAETSEVLKK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=518, Percent_Identity=49.6138996138996, Blast_Score=474, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 56266; Mature: 56266

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNREIVQKLWNLCNVLRDDGITYHQYVTELTYLLFLKMMKETGQEYIIPEKYRWDSLVE
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
KDGIELKEHYQQLLLDLGKEENELLKQIYTDATSNIREPKNLEKIIQSINNLDWYNAKQE
HCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHH
GLGDLYEGLLEKNASEVKSGAGQYFTPRVLIDVIVELVNPQPGERCHDPAAGTFGFMIAA
HHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEC
DRHVREQTDDYFDLSQEEIEFQKYKAFSGVELVRDTHRLAVMNALLHDIHGEILLGDTLS
CHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
SLGESLKNYDVILTNPPFGTKKGGERATRTDFTFTTSNKQLNFLQHIYRALKPNGKARAA
HHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEE
VIVPDNVLFEGGVGADIRRDLMDKCNLHTILRLPTGIFYAQGVKTNVLFFTRGKTDVGNT
EEECCCEEECCCCCHHHHHHHHHHCCCHHHHHCCCHHHEECCCCEEEEEEECCCCCCCCC
KEVWVYDLRTNMPSFGKRNPLTKEHFEGFIKAYTAEDRRKVKDERWNVFTREEIAKNGDS
CEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
LDIGLIADESLSVYENLPDPIDSAEEAVEKLELAISLLNEVIAELKAVEQPNSYQATSKE
EEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ILKVAETSEVLKK
HHHHHHHHHHHCC
>Mature Secondary Structure
MNNREIVQKLWNLCNVLRDDGITYHQYVTELTYLLFLKMMKETGQEYIIPEKYRWDSLVE
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
KDGIELKEHYQQLLLDLGKEENELLKQIYTDATSNIREPKNLEKIIQSINNLDWYNAKQE
HCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHH
GLGDLYEGLLEKNASEVKSGAGQYFTPRVLIDVIVELVNPQPGERCHDPAAGTFGFMIAA
HHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEC
DRHVREQTDDYFDLSQEEIEFQKYKAFSGVELVRDTHRLAVMNALLHDIHGEILLGDTLS
CHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
SLGESLKNYDVILTNPPFGTKKGGERATRTDFTFTTSNKQLNFLQHIYRALKPNGKARAA
HHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEE
VIVPDNVLFEGGVGADIRRDLMDKCNLHTILRLPTGIFYAQGVKTNVLFFTRGKTDVGNT
EEECCCEEECCCCCHHHHHHHHHHCCCHHHHHCCCHHHEECCCCEEEEEEECCCCCCCCC
KEVWVYDLRTNMPSFGKRNPLTKEHFEGFIKAYTAEDRRKVKDERWNVFTREEIAKNGDS
CEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCC
LDIGLIADESLSVYENLPDPIDSAEEAVEKLELAISLLNEVIAELKAVEQPNSYQATSKE
EEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
ILKVAETSEVLKK
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3323532; 7610040; 9278503 [H]