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The map label for this gene is yjbJ [H]

Identifier: 138894378

GI number: 138894378

Start: 785613

End: 786224

Strand: Reverse

Name: yjbJ [H]

Synonym: GTNG_0706

Alternate gene names: 138894378

Gene position: 786224-785613 (Counterclockwise)

Preceding gene: 138894379

Following gene: 138894377

Centisome position: 22.15

GC content: 55.07

Gene sequence:

>612_bases
ATGATGACCGTTTCACCGTTAAAACTGCTTGTCGAATGGCAATCACTCCAAACGCTCTCATCCAGGAGCTCAAGCTTATT
GTCATCACCTGTTCCATCATTGTTTTCCAACTTGCTTGCCGAGTACCTCGAAAGCCAAGCGGAATCGTCAGCTCATGGGT
TGCAGAATTCCACGCCTGCTAGCAGCAGCGATTCCACTAAACGGGACAGTCAGGCGACATCATCCTCGGTCGCGCCGTCT
TCGATTGATGCGCTCATTGACGCTGCGGCCAAAAAATATGATGTCGACCCGCAGCTCGTCCGTGCTGTCATTCGCCACGA
ATCCAACTTCCGCCCGGACGCAAAAAGCCGAGCCGGGGCGCTTGGGTTAATGCAGCTCATGCCGAGCACGGCGAAAATGC
TTGGGGTTGACAATCCGCTTGATCCTGCGCAAAACATCGACGGCGGGGTAAAATATTTGCGTCAGCTTCTTGACCGATAC
GACGGGAATACCACCCTTGCCTTGGCTGCTTATAACGCTGGGCCAGGCCGCGTCGACCGTTACGGCGGGGTCCCACCGTT
TGCCGAAACGAAGGCGTACGTTGAGCGCGTACTGCGAAGCTATCGTGCGTAA

Upstream 100 bases:

>100_bases
AGCGATTTTTGCGCTTGCTCGGGACGCTCGGCATTCCACGCCGCCCGGCACCAAATAAAATCGCCCGCTTTTATGCACGC
AAGTACGAAATGGAGGAAAG

Downstream 100 bases:

>100_bases
ATCGCCGTGTTCAAGTCACTTTGTTTGAGATAAACGTCTGCTGTTGATAAAATAGCAATCAAACGACGACGAATGACTTT
CAAACAAAAGGAGCATCCGC

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MMTVSPLKLLVEWQSLQTLSSRSSSLLSSPVPSLFSNLLAEYLESQAESSAHGLQNSTPASSSDSTKRDSQATSSSVAPS
SIDALIDAAAKKYDVDPQLVRAVIRHESNFRPDAKSRAGALGLMQLMPSTAKMLGVDNPLDPAQNIDGGVKYLRQLLDRY
DGNTTLALAAYNAGPGRVDRYGGVPPFAETKAYVERVLRSYRA

Sequences:

>Translated_203_residues
MMTVSPLKLLVEWQSLQTLSSRSSSLLSSPVPSLFSNLLAEYLESQAESSAHGLQNSTPASSSDSTKRDSQATSSSVAPS
SIDALIDAAAKKYDVDPQLVRAVIRHESNFRPDAKSRAGALGLMQLMPSTAKMLGVDNPLDPAQNIDGGVKYLRQLLDRY
DGNTTLALAAYNAGPGRVDRYGGVPPFAETKAYVERVLRSYRA
>Mature_203_residues
MMTVSPLKLLVEWQSLQTLSSRSSSLLSSPVPSLFSNLLAEYLESQAESSAHGLQNSTPASSSDSTKRDSQATSSSVAPS
SIDALIDAAAKKYDVDPQLVRAVIRHESNFRPDAKSRAGALGLMQLMPSTAKMLGVDNPLDPAQNIDGGVKYLRQLLDRY
DGNTTLALAAYNAGPGRVDRYGGVPPFAETKAYVERVLRSYRA

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=120, Percent_Identity=40.8333333333333, Blast_Score=78, Evalue=3e-16,
Organism=Escherichia coli, GI87082191, Length=118, Percent_Identity=37.2881355932203, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 21820; Mature: 21820

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTVSPLKLLVEWQSLQTLSSRSSSLLSSPVPSLFSNLLAEYLESQAESSAHGLQNSTPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SSSDSTKRDSQATSSSVAPSSIDALIDAAAKKYDVDPQLVRAVIRHESNFRPDAKSRAGA
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHH
LGLMQLMPSTAKMLGVDNPLDPAQNIDGGVKYLRQLLDRYDGNTTLALAAYNAGPGRVDR
HHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
YGGVPPFAETKAYVERVLRSYRA
CCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMTVSPLKLLVEWQSLQTLSSRSSSLLSSPVPSLFSNLLAEYLESQAESSAHGLQNSTPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SSSDSTKRDSQATSSSVAPSSIDALIDAAAKKYDVDPQLVRAVIRHESNFRPDAKSRAGA
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHH
LGLMQLMPSTAKMLGVDNPLDPAQNIDGGVKYLRQLLDRYDGNTTLALAAYNAGPGRVDR
HHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
YGGVPPFAETKAYVERVLRSYRA
CCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]