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The map label for this gene is potD-A [H]

Identifier: 138894300

GI number: 138894300

Start: 699961

End: 701034

Strand: Direct

Name: potD-A [H]

Synonym: GTNG_0626

Alternate gene names: 138894300

Gene position: 699961-701034 (Clockwise)

Preceding gene: 138894299

Following gene: 138894307

Centisome position: 19.72

GC content: 48.88

Gene sequence:

>1074_bases
GTGAAACGGCTTGTTCAAGGATTTGCTGCTATATTCATCGCCGCGTTCGCCCTCATGTTCATGACCCAGCAGCTGAACGA
AGCGGAAGGATATGGAGGCGACAAGACGGTCAGTGTGTACAACTGGGGCGATTACATCGACCCTGCGCTTATCCGGAAGT
TTGAAAAAGAAACCGGCTGGAAAGTGATTTACGAAACGTTCGATTCGAACGAGGCGATGATGGCGAAAATTGCTCAAGGC
GGCGCGACGTTTGATGTTGCCGTTCCGTCCGATTACGCCATCAGCAAAATGATTGAGGAAAACTTGCTCTTGCCGCTTGA
CCATAACAAGCTGCCGAATTTGAAATATATCGAACCGCGGTTTCTCGACTTGTCGTTTGATCCGCATAACCGGTATTCGG
TGCCGTATTTTTGGGGGACGGTTGGCATTGTCTACAACCGCGAGATGCTTGGCGGCAAAACGATCACCGACTGGAACGAT
TTATGGGATCCAAGCTTGCGTAATCAAATTTTGCTTGTCGATGGGGCGCGTGAAGTGATGGGGATGGCATTAAACAGCCT
CGGCTATTCGCTCAATGATACGAACAAGCATCATCTGCGAGAGGCAAAACAAAAGCTCGACCGGCTCATGCCGAATGTGA
AGGCCATTGTTGGCGACGAAATCAAGCTGCTTCTCGTCAATGAAGAGGCGGCCATCGGTGTCGTCTGGTCGGGCGATGCG
GCGGAAATCATTGCCGAAAACGACAAGCTCGATTATGTCGTGCCCGAAGAAGGATCCAACTTATGGTTTGACAATATGGT
AATTCCAAAAACGGCGAAAAACATCGAGGGAGCGCACGCGTTTATCAATTTTATGCTTGATCCAAAACATGCGGCACAAA
ACGCTGAGTATGTCGGCTATTCGACGCCGAATAAAGCGGCGCTTCGCTATTTACCGAAAGACGTCGCCAACGACCAGCGT
TTTTATCCGGATTTAGATTCGGTCGGACGTTTGGAAGTGTATGAAAACTTAGGAAAGCGCATGCTTGCCTACTACAATGA
GCTGTTTTTGGAGTTTAAGATGCATCAAAAGTAA

Upstream 100 bases:

>100_bases
GTCGACATTGCTGTTTTTGTTTACGATGTTGCTTGTCGTCGGCTATTACGTGCTCAGCCAAAAAGGCGGGAGAATGTATG
GGATAGGGGGGCGGAAGTAG

Downstream 100 bases:

>100_bases
ACAATCACCGGATGAGCGAGTGCGTGTCTAGAAAAACTCGACACGCGCTCGCTTTTTGTTGAAAAAGTTAGTGCTTTCTT
TTTCTCGGGATCGTCAGTGT

Product: spermidine/putrescine ABC transporter spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKRLVQGFAAIFIAAFALMFMTQQLNEAEGYGGDKTVSVYNWGDYIDPALIRKFEKETGWKVIYETFDSNEAMMAKIAQG
GATFDVAVPSDYAISKMIEENLLLPLDHNKLPNLKYIEPRFLDLSFDPHNRYSVPYFWGTVGIVYNREMLGGKTITDWND
LWDPSLRNQILLVDGAREVMGMALNSLGYSLNDTNKHHLREAKQKLDRLMPNVKAIVGDEIKLLLVNEEAAIGVVWSGDA
AEIIAENDKLDYVVPEEGSNLWFDNMVIPKTAKNIEGAHAFINFMLDPKHAAQNAEYVGYSTPNKAALRYLPKDVANDQR
FYPDLDSVGRLEVYENLGKRMLAYYNELFLEFKMHQK

Sequences:

>Translated_357_residues
MKRLVQGFAAIFIAAFALMFMTQQLNEAEGYGGDKTVSVYNWGDYIDPALIRKFEKETGWKVIYETFDSNEAMMAKIAQG
GATFDVAVPSDYAISKMIEENLLLPLDHNKLPNLKYIEPRFLDLSFDPHNRYSVPYFWGTVGIVYNREMLGGKTITDWND
LWDPSLRNQILLVDGAREVMGMALNSLGYSLNDTNKHHLREAKQKLDRLMPNVKAIVGDEIKLLLVNEEAAIGVVWSGDA
AEIIAENDKLDYVVPEEGSNLWFDNMVIPKTAKNIEGAHAFINFMLDPKHAAQNAEYVGYSTPNKAALRYLPKDVANDQR
FYPDLDSVGRLEVYENLGKRMLAYYNELFLEFKMHQK
>Mature_357_residues
MKRLVQGFAAIFIAAFALMFMTQQLNEAEGYGGDKTVSVYNWGDYIDPALIRKFEKETGWKVIYETFDSNEAMMAKIAQG
GATFDVAVPSDYAISKMIEENLLLPLDHNKLPNLKYIEPRFLDLSFDPHNRYSVPYFWGTVGIVYNREMLGGKTITDWND
LWDPSLRNQILLVDGAREVMGMALNSLGYSLNDTNKHHLREAKQKLDRLMPNVKAIVGDEIKLLLVNEEAAIGVVWSGDA
AEIIAENDKLDYVVPEEGSNLWFDNMVIPKTAKNIEGAHAFINFMLDPKHAAQNAEYVGYSTPNKAALRYLPKDVANDQR
FYPDLDSVGRLEVYENLGKRMLAYYNELFLEFKMHQK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=322, Percent_Identity=40.9937888198758, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1787078, Length=338, Percent_Identity=32.2485207100592, Blast_Score=168, Evalue=5e-43,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40646; Mature: 40646

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLVQGFAAIFIAAFALMFMTQQLNEAEGYGGDKTVSVYNWGDYIDPALIRKFEKETGW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
KVIYETFDSNEAMMAKIAQGGATFDVAVPSDYAISKMIEENLLLPLDHNKLPNLKYIEPR
EEEEEECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCEEEECCCCCCCCEECCCE
FLDLSFDPHNRYSVPYFWGTVGIVYNREMLGGKTITDWNDLWDPSLRNQILLVDGAREVM
EEEEEECCCCCEECCEEECCEEEEECCHHCCCCEECCCHHHCCCCCCCEEEEEECHHHHH
GMALNSLGYSLNDTNKHHLREAKQKLDRLMPNVKAIVGDEIKLLLVNEEAAIGVVWSGDA
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHEECCCCEEEEEEECCCEEEEEECCCC
AEIIAENDKLDYVVPEEGSNLWFDNMVIPKTAKNIEGAHAFINFMLDPKHAAQNAEYVGY
CEEEECCCCEEEEECCCCCCEEECCEECCCCCCCCCHHEEEEEEEECCHHHHCCCCEECC
STPNKAALRYLPKDVANDQRFYPDLDSVGRLEVYENLGKRMLAYYNELFLEFKMHQK
CCCCHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MKRLVQGFAAIFIAAFALMFMTQQLNEAEGYGGDKTVSVYNWGDYIDPALIRKFEKETGW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
KVIYETFDSNEAMMAKIAQGGATFDVAVPSDYAISKMIEENLLLPLDHNKLPNLKYIEPR
EEEEEECCCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCEEEECCCCCCCCEECCCE
FLDLSFDPHNRYSVPYFWGTVGIVYNREMLGGKTITDWNDLWDPSLRNQILLVDGAREVM
EEEEEECCCCCEECCEEECCEEEEECCHHCCCCEECCCHHHCCCCCCCEEEEEECHHHHH
GMALNSLGYSLNDTNKHHLREAKQKLDRLMPNVKAIVGDEIKLLLVNEEAAIGVVWSGDA
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHEECCCCEEEEEEECCCEEEEEECCCC
AEIIAENDKLDYVVPEEGSNLWFDNMVIPKTAKNIEGAHAFINFMLDPKHAAQNAEYVGY
CEEEECCCCEEEEECCCCCCEEECCEECCCCCCCCCHHEEEEEEEECCHHHHCCCCEECC
STPNKAALRYLPKDVANDQRFYPDLDSVGRLEVYENLGKRMLAYYNELFLEFKMHQK
CCCCHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]