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The map label for this gene is pdhC [H]

Identifier: 138894293

GI number: 138894293

Start: 688556

End: 689866

Strand: Direct

Name: pdhC [H]

Synonym: GTNG_0619

Alternate gene names: 138894293

Gene position: 688556-689866 (Clockwise)

Preceding gene: 138894292

Following gene: 138894297

Centisome position: 19.39

GC content: 48.05

Gene sequence:

>1311_bases
ATGTTTGTCGAAGTGACATTGCCCAAACTATCCGATTCCCATGATGAAAGTTTAATTACGTTTTGGCACGTTTCCGAAGG
AGATGCGGTGGAAAAAGGAGCCACGCTAGTGGAAGTGCAAACCGAAAAGGCCGTTTCAGAAATTCACGCTCCTGAGTCGG
GAACAGTAAAAGAGATCAAAAAGAAACGAGGGGATACGGCAAAGGTGGGAGAAGTGCTGGCTGTCCTTGCCGTTGAAGCG
TTTGCTCCGGACAGCGGGGGTCCGCAGATAGAGATCAAGATCACCCCGCGCGTAAAAAAATTGGCCAAGGAGCTTGGTGT
CGATTGGCGTGCGATCACACCGACAGGAGCGAATGGCAAAATCACGGAAGACGATATTCGTCGTTCGGTGAGCACGAGAA
GACAAGAGGTTTCACAAAAAAAAATTGTGGCTGCCCCATCAGTGCGAAAGTTTGCTCGCGAGCAGAACGTGCCGTTAGAG
GAAGTCACTCCGAGCGGAAAGAACGGGAGAATATTGAAAAGCGATATTGAAGCGGTATTATCGGTTCAGCAACGAAAAGC
AACAGATGAAGCTGCTGCCAGTGTTGAGATTGTCAAGAAGCAGGAAAGCCAAGAAAAAGTGCGTCGAGTGCCGTTGACAG
GCATCCGTAAGGCTATCACCCAAGCGATGGTGCGGTCAACGCGGACTATTCCACAAGTCACCCATTTTAGCGAGGCCAAT
GCGACCCGACTTGTTCAACATCGTCAACGGATCAAACCTCTAGCAGAGCAACAAGGGATGAAACTGACCTATTTAGTCTA
TGTGATAAAAGCGCTGGCAGCTGTGTTAAAAAAGTATCCAATGCTCAATGCTTCCCTTGATGAGGAACAAGAGGAAATTG
TCATTCATGAATCGATTCATATTGGGTTTGCGGTTGACACTGATCGAGGATTGCTCGTTCCAGTGATTCGTGACGCGGAT
CAAAAATCGTTGTTTCAGATTGCCAAAGAAATCGAAGAATTGTCAGCGAAGGCAAGAACGGGGGCGATTCAAGCAGCGGA
GATGTCCGGAGGGACGTGCACGGTTTCAAACATCGGCTCGGCCAACGGCTCGTGGTTCACGCCGATTATCCATTATCCGC
AGTCTTGCATTTTAGGAATCGGTAAAATTGATAAAAAGCCAATAGTCATCAATGACTCGATTGAGATCTCCTTCGTGATG
CCGCTATCCCTTACTTATGATCATCGTCTGATCGATGGGGTCATGGCGCAACATGCCCTAAATGAATGTCAAACCTACTT
GTCTGAACCAGATTGGCTGTTCGTTATGTGA

Upstream 100 bases:

>100_bases
CGCTTGAACAATTTGTTTTGCCGAATGCCGATAAAATTTTCCATGAGGCAATTAAGCTTATCAATGAATAAGGAAAAGTT
TGTTCCAAGGGAGGCAGCTT

Downstream 100 bases:

>100_bases
CAAAAAACCACCCGCCCCATCCGGGACGGGCTGAGAGAAGAGGGGCACCTTGTCAAGAAGTAGAGGAACATCATAGCTTT
ATCGTACGCGTTTTTTTACC

Product: pyruvate dehydrogenase E2 (dihydrolipoamideacetyltransferase)

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MFVEVTLPKLSDSHDESLITFWHVSEGDAVEKGATLVEVQTEKAVSEIHAPESGTVKEIKKKRGDTAKVGEVLAVLAVEA
FAPDSGGPQIEIKITPRVKKLAKELGVDWRAITPTGANGKITEDDIRRSVSTRRQEVSQKKIVAAPSVRKFAREQNVPLE
EVTPSGKNGRILKSDIEAVLSVQQRKATDEAAASVEIVKKQESQEKVRRVPLTGIRKAITQAMVRSTRTIPQVTHFSEAN
ATRLVQHRQRIKPLAEQQGMKLTYLVYVIKALAAVLKKYPMLNASLDEEQEEIVIHESIHIGFAVDTDRGLLVPVIRDAD
QKSLFQIAKEIEELSAKARTGAIQAAEMSGGTCTVSNIGSANGSWFTPIIHYPQSCILGIGKIDKKPIVINDSIEISFVM
PLSLTYDHRLIDGVMAQHALNECQTYLSEPDWLFVM

Sequences:

>Translated_436_residues
MFVEVTLPKLSDSHDESLITFWHVSEGDAVEKGATLVEVQTEKAVSEIHAPESGTVKEIKKKRGDTAKVGEVLAVLAVEA
FAPDSGGPQIEIKITPRVKKLAKELGVDWRAITPTGANGKITEDDIRRSVSTRRQEVSQKKIVAAPSVRKFAREQNVPLE
EVTPSGKNGRILKSDIEAVLSVQQRKATDEAAASVEIVKKQESQEKVRRVPLTGIRKAITQAMVRSTRTIPQVTHFSEAN
ATRLVQHRQRIKPLAEQQGMKLTYLVYVIKALAAVLKKYPMLNASLDEEQEEIVIHESIHIGFAVDTDRGLLVPVIRDAD
QKSLFQIAKEIEELSAKARTGAIQAAEMSGGTCTVSNIGSANGSWFTPIIHYPQSCILGIGKIDKKPIVINDSIEISFVM
PLSLTYDHRLIDGVMAQHALNECQTYLSEPDWLFVM
>Mature_436_residues
MFVEVTLPKLSDSHDESLITFWHVSEGDAVEKGATLVEVQTEKAVSEIHAPESGTVKEIKKKRGDTAKVGEVLAVLAVEA
FAPDSGGPQIEIKITPRVKKLAKELGVDWRAITPTGANGKITEDDIRRSVSTRRQEVSQKKIVAAPSVRKFAREQNVPLE
EVTPSGKNGRILKSDIEAVLSVQQRKATDEAAASVEIVKKQESQEKVRRVPLTGIRKAITQAMVRSTRTIPQVTHFSEAN
ATRLVQHRQRIKPLAEQQGMKLTYLVYVIKALAAVLKKYPMLNASLDEEQEEIVIHESIHIGFAVDTDRGLLVPVIRDAD
QKSLFQIAKEIEELSAKARTGAIQAAEMSGGTCTVSNIGSANGSWFTPIIHYPQSCILGIGKIDKKPIVINDSIEISFVM
PLSLTYDHRLIDGVMAQHALNECQTYLSEPDWLFVM

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=451, Percent_Identity=30.1552106430155, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI19923748, Length=238, Percent_Identity=36.1344537815126, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI203098816, Length=228, Percent_Identity=29.3859649122807, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI203098753, Length=228, Percent_Identity=29.3859649122807, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=31.1377245508982, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786946, Length=437, Percent_Identity=28.8329519450801, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1786305, Length=300, Percent_Identity=33.3333333333333, Blast_Score=173, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=448, Percent_Identity=29.0178571428571, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17560088, Length=453, Percent_Identity=29.5805739514349, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=229, Percent_Identity=37.117903930131, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17538894, Length=313, Percent_Identity=25.5591054313099, Blast_Score=96, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=433, Percent_Identity=28.8683602771363, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=26.2582056892779, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI18859875, Length=452, Percent_Identity=30.5309734513274, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24582497, Length=294, Percent_Identity=28.2312925170068, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI20129315, Length=294, Percent_Identity=28.2312925170068, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=33.9285714285714, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 48066; Mature: 48066

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVEVTLPKLSDSHDESLITFWHVSEGDAVEKGATLVEVQTEKAVSEIHAPESGTVKEIK
CEEEEECCCCCCCCCCCEEEEEECCCCCCHHCCCEEEEEHHHHHHHHHCCCCCCCHHHHH
KKRGDTAKVGEVLAVLAVEAFAPDSGGPQIEIKITPRVKKLAKELGVDWRAITPTGANGK
HHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHCCCEEEECCCCCCCC
ITEDDIRRSVSTRRQEVSQKKIVAAPSVRKFAREQNVPLEEVTPSGKNGRILKSDIEAVL
CCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCHHHCCCCCCCCCEEHHHHHHHH
SVQQRKATDEAAASVEIVKKQESQEKVRRVPLTGIRKAITQAMVRSTRTIPQVTHFSEAN
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
ATRLVQHRQRIKPLAEQQGMKLTYLVYVIKALAAVLKKYPMLNASLDEEQEEIVIHESIH
HHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEE
IGFAVDTDRGLLVPVIRDADQKSLFQIAKEIEELSAKARTGAIQAAEMSGGTCTVSNIGS
EEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCC
ANGSWFTPIIHYPQSCILGIGKIDKKPIVINDSIEISFVMPLSLTYDHRLIDGVMAQHAL
CCCCEECHHHHCCHHHHEECCCCCCCCEEEECCEEEEEEEEEEECCCHHHHHHHHHHHHH
NECQTYLSEPDWLFVM
HHHHHHHCCCCEEEEC
>Mature Secondary Structure
MFVEVTLPKLSDSHDESLITFWHVSEGDAVEKGATLVEVQTEKAVSEIHAPESGTVKEIK
CEEEEECCCCCCCCCCCEEEEEECCCCCCHHCCCEEEEEHHHHHHHHHCCCCCCCHHHHH
KKRGDTAKVGEVLAVLAVEAFAPDSGGPQIEIKITPRVKKLAKELGVDWRAITPTGANGK
HHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHCCCEEEECCCCCCCC
ITEDDIRRSVSTRRQEVSQKKIVAAPSVRKFAREQNVPLEEVTPSGKNGRILKSDIEAVL
CCHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCHHHCCCCCCCCCEEHHHHHHHH
SVQQRKATDEAAASVEIVKKQESQEKVRRVPLTGIRKAITQAMVRSTRTIPQVTHFSEAN
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
ATRLVQHRQRIKPLAEQQGMKLTYLVYVIKALAAVLKKYPMLNASLDEEQEEIVIHESIH
HHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEE
IGFAVDTDRGLLVPVIRDADQKSLFQIAKEIEELSAKARTGAIQAAEMSGGTCTVSNIGS
EEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCC
ANGSWFTPIIHYPQSCILGIGKIDKKPIVINDSIEISFVMPLSLTYDHRLIDGVMAQHAL
CCCCEECHHHHCCHHHHEECCCCCCCCEEEECCEEEEEEEEEEECCCHHHHHHHHHHHHH
NECQTYLSEPDWLFVM
HHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]