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The map label for this gene is tenI [H]

Identifier: 138894209

GI number: 138894209

Start: 598018

End: 598629

Strand: Direct

Name: tenI [H]

Synonym: GTNG_0535

Alternate gene names: 138894209

Gene position: 598018-598629 (Clockwise)

Preceding gene: 138894208

Following gene: 138894210

Centisome position: 16.84

GC content: 57.84

Gene sequence:

>612_bases
ATGGGAACGCTTCATTTCGTTTCGACCGGTCGGCAGACGGCCGATGAGTTCGCCGCCATTTGTCAGCACATCCATCCGTA
CGCCGATTGCATTCATATTCGTGAAAAAGAGAAAACAGCGCGCGAGGTTGCCGCGTTTGTCACTGCGCTGCTTCGTGTCG
GGGTGCCACCCCAAAAAATCATCGTCAATGACCGCGTCGATGTGGCTGCAGTGTATGGCGTCCATGGAGTGCAACTCGCC
TACCATAGCCTACCGGTGCGGGCTGTCCGCCGCTCATTTCCGAGTTTGACAGTCGGATGCTCGGTGCATGGGCTGGCGGA
GGCGAAGCAGGCGGAAGAGGACGGTGCCCACTTTTGTTTATTCGGCCATATTTTTCCGACCGCCAGCAAACCGGGGGTGC
TTCCGCGCGGCGTAGACTTGCTGAAGGAAATAGCCGCTGCTGTACATATTCCCGTCATTGCCATCGGCGGCATCCATGCC
GGCAATGCCCGCCGAGTGCTAGAAGCGGGGGCAGCGGGAGTGGCGGTGTTGTCGGCGATTTTTTTCGCAGCTGATCCGGT
CAGTGAAGCGAAACGGCTAGCTGAAATTGTCAAGGGGGAGAGGATCAAGTGA

Upstream 100 bases:

>100_bases
GTTATTTTGTTTGTCGTTTTGTTATTTGTTTCCTATCATGTTGGCATTCATTATCCGTACATTTCTATAAACGATGTACG
GTAGAAAGAAAGGGATCTAG

Downstream 100 bases:

>100_bases
CTATACATTATTATGACGTTGCCATTGTTGGCGGTGGGGTGATCGGGGCGGCGGTGGCCTTCGAGCTTGCCAAACGGAAG
TATCGCGTCGCGATTTTAGA

Product: transcriptional regulator TenI

Products: diphosphate; thiamine monophosphate

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MGTLHFVSTGRQTADEFAAICQHIHPYADCIHIREKEKTAREVAAFVTALLRVGVPPQKIIVNDRVDVAAVYGVHGVQLA
YHSLPVRAVRRSFPSLTVGCSVHGLAEAKQAEEDGAHFCLFGHIFPTASKPGVLPRGVDLLKEIAAAVHIPVIAIGGIHA
GNARRVLEAGAAGVAVLSAIFFAADPVSEAKRLAEIVKGERIK

Sequences:

>Translated_203_residues
MGTLHFVSTGRQTADEFAAICQHIHPYADCIHIREKEKTAREVAAFVTALLRVGVPPQKIIVNDRVDVAAVYGVHGVQLA
YHSLPVRAVRRSFPSLTVGCSVHGLAEAKQAEEDGAHFCLFGHIFPTASKPGVLPRGVDLLKEIAAAVHIPVIAIGGIHA
GNARRVLEAGAAGVAVLSAIFFAADPVSEAKRLAEIVKGERIK
>Mature_202_residues
GTLHFVSTGRQTADEFAAICQHIHPYADCIHIREKEKTAREVAAFVTALLRVGVPPQKIIVNDRVDVAAVYGVHGVQLAY
HSLPVRAVRRSFPSLTVGCSVHGLAEAKQAEEDGAHFCLFGHIFPTASKPGVLPRGVDLLKEIAAAVHIPVIAIGGIHAG
NARRVLEAGAAGVAVLSAIFFAADPVSEAKRLAEIVKGERIK

Specific function: Reduces the stimulatory effect of the tenA transcriptional activator on production of certain extracellular enzymes. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: 2.5.1.3

Molecular weight: Translated: 21573; Mature: 21442

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLHFVSTGRQTADEFAAICQHIHPYADCIHIREKEKTAREVAAFVTALLRVGVPPQKI
CCCEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHE
IVNDRVDVAAVYGVHGVQLAYHSLPVRAVRRSFPSLTVGCSVHGLAEAKQAEEDGAHFCL
EEECCCCEEHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCEEEE
FGHIFPTASKPGVLPRGVDLLKEIAAAVHIPVIAIGGIHAGNARRVLEAGAAGVAVLSAI
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCHHHHHHHHH
FFAADPVSEAKRLAEIVKGERIK
HHHCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GTLHFVSTGRQTADEFAAICQHIHPYADCIHIREKEKTAREVAAFVTALLRVGVPPQKI
CCEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHE
IVNDRVDVAAVYGVHGVQLAYHSLPVRAVRRSFPSLTVGCSVHGLAEAKQAEEDGAHFCL
EEECCCCEEHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCEEEE
FGHIFPTASKPGVLPRGVDLLKEIAAAVHIPVIAIGGIHAGNARRVLEAGAAGVAVLSAI
EEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCHHHHHHHHH
FFAADPVSEAKRLAEIVKGERIK
HHHCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0004 {thiazole} 0.00085 {hydroxymethylpyrimidine} [C]

Substrates: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphono-oxyethyl)-thiazole

Specific reaction: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine monophosphate

General reaction: Hydroxymethylpyrimidine group transfer [C]

Inhibitor: Acetyl phosphate substrates; ADP; ATP; CTP; Diphosphate; GTP; Phosphocreatine; Phosphoenolpyruvate; UTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]