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The map label for this gene is yfkE [H]

Identifier: 138894069

GI number: 138894069

Start: 444783

End: 445835

Strand: Direct

Name: yfkE [H]

Synonym: GTNG_0395

Alternate gene names: 138894069

Gene position: 444783-445835 (Clockwise)

Preceding gene: 138894066

Following gene: 138894070

Centisome position: 12.53

GC content: 49.76

Gene sequence:

>1053_bases
ATGAACAAGTTGTTTGCGTGGATGACATGGATCGGCGTGCCAGTATCCGTCATCGGTGGCTTTCTCCACTGGCCGACCGT
TTTGATGTTTGTGTTGTACTGTTTAACGATTGTCGCTCTGGCCAGCTATATGGGGAGAGCGACAGAGAGTTTAGCGATCG
TCGCTGGACCGCGCATCGGCGGACTTTTGAACGCTACGTTTGGCAACGCAGTGGAGCTTATTATCTCCATTTTTGCCTTG
CGGGCCGGGCTCGTTGATGTGGTGCTTGCTTCCTTGACTGGTTCAGTGCTCGGGAACTTGCTTTTAGTGGCGGGGTTATC
GTTTTTTGTCGGCGGCCTGAAATACAAACGGCAAGAGTTCAACATCTATGATGCTCGTCATAACGCGGGCCTGCTCACCT
TTGCCATTTTTGTTGCGTTCGTTATCCCCGAAGTGTTCACGGCGGAGATGGCACCTGACGAACGGCTTGTATTAAGCGTC
GGCATTTCAATCATTATGATTTTGCTTTATCTAGCCGCTCTTTACTTCCGGCTCGTCACTCACCGCGGTGTTTATCAGCA
TAAATCGGATGAAGTGGAAGAACATGAGGAACCGGAATGGGGAAGAGGAAAAGCGACTATTATTTTAGCGCTGGCGACGG
CGGCAGTTGCTTACTTATCGGAGCGGCTTGTCCATACATTTGAAACAGTTGCTGAATCGTTCGGCTGGAGCGAGTTGTTT
ATTGGGGTGATTATCGTCGCGATCGTCGGTAATGCGGCTGAGCACGCTTCCGCCATTATTATGGCGTATAAAAACAAAAT
GAATGTCGCTGTGGAAATCGCGGTCGGATCGACATTGCAAATCGCCATGTTCGTCGCTCCTGTGCTTGTGCTTGTCTCAC
TTTTGTTTCCGGAAAAAATGCCGCTTGTCTTTTCGCTTCCTGAGCTTATCGCGATGGGGGCTGCAGTCCTTTTGACGATC
GTGATCTCTAATGATGGGGATACGAACTGGTTTGAGGGAGCAACACTGTTAGCGGCATATACGATTATGGGAATCGGCTT
TTACTTGCTGTAG

Upstream 100 bases:

>100_bases
CGCTTTTTTACGGACCAATGTATCGTTTCGTATTCATCGTACATATGAATGATGGCCAATGCAACGAAAAACGTTTCGAA
GAAAGGTGGAATGAAAACGA

Downstream 100 bases:

>100_bases
TCATTGCATCCGGGCATTCGCTGCCCGGGTGTTTTTTTGGCGGATGCGGGGAAATAAGGTGCCAAAGTAGGATGAAATTT
TCACCGCGACGGATAAAGGA

Product: calcium/proton exchanger

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNKLFAWMTWIGVPVSVIGGFLHWPTVLMFVLYCLTIVALASYMGRATESLAIVAGPRIGGLLNATFGNAVELIISIFAL
RAGLVDVVLASLTGSVLGNLLLVAGLSFFVGGLKYKRQEFNIYDARHNAGLLTFAIFVAFVIPEVFTAEMAPDERLVLSV
GISIIMILLYLAALYFRLVTHRGVYQHKSDEVEEHEEPEWGRGKATIILALATAAVAYLSERLVHTFETVAESFGWSELF
IGVIIVAIVGNAAEHASAIIMAYKNKMNVAVEIAVGSTLQIAMFVAPVLVLVSLLFPEKMPLVFSLPELIAMGAAVLLTI
VISNDGDTNWFEGATLLAAYTIMGIGFYLL

Sequences:

>Translated_350_residues
MNKLFAWMTWIGVPVSVIGGFLHWPTVLMFVLYCLTIVALASYMGRATESLAIVAGPRIGGLLNATFGNAVELIISIFAL
RAGLVDVVLASLTGSVLGNLLLVAGLSFFVGGLKYKRQEFNIYDARHNAGLLTFAIFVAFVIPEVFTAEMAPDERLVLSV
GISIIMILLYLAALYFRLVTHRGVYQHKSDEVEEHEEPEWGRGKATIILALATAAVAYLSERLVHTFETVAESFGWSELF
IGVIIVAIVGNAAEHASAIIMAYKNKMNVAVEIAVGSTLQIAMFVAPVLVLVSLLFPEKMPLVFSLPELIAMGAAVLLTI
VISNDGDTNWFEGATLLAAYTIMGIGFYLL
>Mature_350_residues
MNKLFAWMTWIGVPVSVIGGFLHWPTVLMFVLYCLTIVALASYMGRATESLAIVAGPRIGGLLNATFGNAVELIISIFAL
RAGLVDVVLASLTGSVLGNLLLVAGLSFFVGGLKYKRQEFNIYDARHNAGLLTFAIFVAFVIPEVFTAEMAPDERLVLSV
GISIIMILLYLAALYFRLVTHRGVYQHKSDEVEEHEEPEWGRGKATIILALATAAVAYLSERLVHTFETVAESFGWSELF
IGVIIVAIVGNAAEHASAIIMAYKNKMNVAVEIAVGSTLQIAMFVAPVLVLVSLLFPEKMPLVFSLPELIAMGAAVLLTI
VISNDGDTNWFEGATLLAAYTIMGIGFYLL

Specific function: According To Ref.1 The Natural Activity Of This Protein Is As A Calcium/Proton Antiporter, But It Can Act As A Weak Sodium/Proton Antiporter. The Presence Of A Preferred Substrate And Normal Ph Cytoplasmic Values Might Prevent Significant Sodium/Proton An

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320075, Length=351, Percent_Identity=39.6011396011396, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6324008, Length=389, Percent_Identity=27.2493573264781, Blast_Score=133, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004798
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 37893; Mature: 37893

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLFAWMTWIGVPVSVIGGFLHWPTVLMFVLYCLTIVALASYMGRATESLAIVAGPRIG
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCEEEEECCCCC
GLLNATFGNAVELIISIFALRAGLVDVVLASLTGSVLGNLLLVAGLSFFVGGLKYKRQEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NIYDARHNAGLLTFAIFVAFVIPEVFTAEMAPDERLVLSVGISIIMILLYLAALYFRLVT
CEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HRGVYQHKSDEVEEHEEPEWGRGKATIILALATAAVAYLSERLVHTFETVAESFGWSELF
HCCHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IGVIIVAIVGNAAEHASAIIMAYKNKMNVAVEIAVGSTLQIAMFVAPVLVLVSLLFPEKM
HHHHHHHHHCCCHHHHHHHHHEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
PLVFSLPELIAMGAAVLLTIVISNDGDTNWFEGATLLAAYTIMGIGFYLL
CHHCCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKLFAWMTWIGVPVSVIGGFLHWPTVLMFVLYCLTIVALASYMGRATESLAIVAGPRIG
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCEEEEECCCCC
GLLNATFGNAVELIISIFALRAGLVDVVLASLTGSVLGNLLLVAGLSFFVGGLKYKRQEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NIYDARHNAGLLTFAIFVAFVIPEVFTAEMAPDERLVLSVGISIIMILLYLAALYFRLVT
CEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HRGVYQHKSDEVEEHEEPEWGRGKATIILALATAAVAYLSERLVHTFETVAESFGWSELF
HCCHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IGVIIVAIVGNAAEHASAIIMAYKNKMNVAVEIAVGSTLQIAMFVAPVLVLVSLLFPEKM
HHHHHHHHHCCCHHHHHHHHHEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
PLVFSLPELIAMGAAVLLTIVISNDGDTNWFEGATLLAAYTIMGIGFYLL
CHHCCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]