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The map label for this gene is glcF [H]

Identifier: 138894046

GI number: 138894046

Start: 423534

End: 424898

Strand: Direct

Name: glcF [H]

Synonym: GTNG_0372

Alternate gene names: 138894046

Gene position: 423534-424898 (Clockwise)

Preceding gene: 138894045

Following gene: 138894048

Centisome position: 11.93

GC content: 51.94

Gene sequence:

>1365_bases
ATGACAACGAAGACAGAACAAGCGCACATTCAAAAAGCGTTTCAAGAACGGATGGATGAGGATGAATTAATGAACTGCAT
GCGCTGTGGTTTTTGTTTGCCGTCGTGTCCAACATACATTGAATCCGGACAGCAAGAAACCCAGTCGCCGCGCGGCCGGA
TCGCCTTAATGAAAGCAGTTGTGGATGGGCTGATCGAGCCGGATGAGGAAGTCGAGCGGTCGCTTAGCTTATGTTTAGGC
TGCCGGGCATGTGAACAAGTTTGTCCGTCTGGCGTCCGCTATGGCCATTTGCTTGAAGAGGCGCGCGATATTATCGCGCA
GCACAAGCGAATGCCGCTTCACGTCCGTCTTGTGCGCAAGCTTGTATTTGCCGGGCTGTTTCCATATCAAGAGCGAATGC
GAAAGGCGACCGCATTTCTCGGTTGGTATCAGCGTTCCGGCTTGCGGGCAATTGTCCGCAAAACTGGTCTGCTTCGCCTG
TTGCCGGCATCGTTCCGGCAAATGGAGCGCGTGCTGCCGGACGTGCCGACGGCAAAAGAGATGAAAAATCGGCCACATCG
CCTGAATCCGGATGAGCCAGCAAAAAAACGAGTCGCTTTTTTCACCGGCTGTTTGATGGATACGATGTTTATGACAACAA
ACGATGCGACGATGCGTCTATTGCAGCTCGCCGGCTGTGAAGTCATCATTCCGCCATCTCAAGGTTGCTGTGGAGCGTTG
CACGGTCATGGGGGGGAAAAAGAGATGGCGAAAGAGTTAGCGAAACGAAATATTGCTGCTTTTGAACAGTTGGACGTCGA
CTATATTGTGACAAACGCAGGCGGTTGTGGTGCGTTTTTACTTGAATACGATCACTTGCTGAAGGACGATCCAGAATGGT
GCGATCGGGCGGCGGCGTTTGCTGCGAAGATCAAAGATGTCTCTGAAGTGCTTGTCGAACTCGAGTTTCATCAACTGCCG
CTTCGCGTTGCGCCGCAAACGATCACCTACCAAGATTCGTGCCATTTGCGAAACGTGATGAAAACAGCATCAGCTCCGCG
CCGCCTGCTGCGAGCGATTGAAGGGGCTGAGTTTCGTGAAATGAAAGATGCCGATCGCTGCTGTGGTTCGGCCGGCATTT
ACAATCTCGTTGAGCCAGAAATGTCGATGCAAATTTTAGATTATAAAATGGAAATGGCGAAACAAACGAAAGCCACGACG
ATCGTCACCGCGAATCCAGGGTGTTTGCTGCAAATGAAGCTTGGCATCGAGCGAGCCGGGTTGACAGAAAGCGTCCGTGC
GGTCCATATTGTGGATTTGTTGCTTGAGGCGGCTGAAGGAGCACAAGCAGTAAGAGAGGAAGAACGCATCCGGCAAACGT
CGTAA

Upstream 100 bases:

>100_bases
CTGCCATGAAAGCGATCAAACAGGCGCTCGACCCGAACAACATTATGAATCCAGGCAAAGTGTTCGCTAAAAGCACGAGA
AAACGGGTGGTGGTGACACG

Downstream 100 bases:

>100_bases
TGCGAGGTCGGTGTAAACCTTTCCGTGTGGATCGGCGGCTCCAACGCGACGAAAGCGCGAAGGCAACTGTTCATTCATAT
GAAAATGAAAACCCCCGCGT

Product: glycolate oxidase, iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTTKTEQAHIQKAFQERMDEDELMNCMRCGFCLPSCPTYIESGQQETQSPRGRIALMKAVVDGLIEPDEEVERSLSLCLG
CRACEQVCPSGVRYGHLLEEARDIIAQHKRMPLHVRLVRKLVFAGLFPYQERMRKATAFLGWYQRSGLRAIVRKTGLLRL
LPASFRQMERVLPDVPTAKEMKNRPHRLNPDEPAKKRVAFFTGCLMDTMFMTTNDATMRLLQLAGCEVIIPPSQGCCGAL
HGHGGEKEMAKELAKRNIAAFEQLDVDYIVTNAGGCGAFLLEYDHLLKDDPEWCDRAAAFAAKIKDVSEVLVELEFHQLP
LRVAPQTITYQDSCHLRNVMKTASAPRRLLRAIEGAEFREMKDADRCCGSAGIYNLVEPEMSMQILDYKMEMAKQTKATT
IVTANPGCLLQMKLGIERAGLTESVRAVHIVDLLLEAAEGAQAVREEERIRQTS

Sequences:

>Translated_454_residues
MTTKTEQAHIQKAFQERMDEDELMNCMRCGFCLPSCPTYIESGQQETQSPRGRIALMKAVVDGLIEPDEEVERSLSLCLG
CRACEQVCPSGVRYGHLLEEARDIIAQHKRMPLHVRLVRKLVFAGLFPYQERMRKATAFLGWYQRSGLRAIVRKTGLLRL
LPASFRQMERVLPDVPTAKEMKNRPHRLNPDEPAKKRVAFFTGCLMDTMFMTTNDATMRLLQLAGCEVIIPPSQGCCGAL
HGHGGEKEMAKELAKRNIAAFEQLDVDYIVTNAGGCGAFLLEYDHLLKDDPEWCDRAAAFAAKIKDVSEVLVELEFHQLP
LRVAPQTITYQDSCHLRNVMKTASAPRRLLRAIEGAEFREMKDADRCCGSAGIYNLVEPEMSMQILDYKMEMAKQTKATT
IVTANPGCLLQMKLGIERAGLTESVRAVHIVDLLLEAAEGAQAVREEERIRQTS
>Mature_453_residues
TTKTEQAHIQKAFQERMDEDELMNCMRCGFCLPSCPTYIESGQQETQSPRGRIALMKAVVDGLIEPDEEVERSLSLCLGC
RACEQVCPSGVRYGHLLEEARDIIAQHKRMPLHVRLVRKLVFAGLFPYQERMRKATAFLGWYQRSGLRAIVRKTGLLRLL
PASFRQMERVLPDVPTAKEMKNRPHRLNPDEPAKKRVAFFTGCLMDTMFMTTNDATMRLLQLAGCEVIIPPSQGCCGALH
GHGGEKEMAKELAKRNIAAFEQLDVDYIVTNAGGCGAFLLEYDHLLKDDPEWCDRAAAFAAKIKDVSEVLVELEFHQLPL
RVAPQTITYQDSCHLRNVMKTASAPRRLLRAIEGAEFREMKDADRCCGSAGIYNLVEPEMSMQILDYKMEMAKQTKATTI
VTANPGCLLQMKLGIERAGLTESVRAVHIVDLLLEAAEGAQAVREEERIRQTS

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=421, Percent_Identity=32.7790973871734, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1786497, Length=241, Percent_Identity=29.045643153527, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 51021; Mature: 50890

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTKTEQAHIQKAFQERMDEDELMNCMRCGFCLPSCPTYIESGQQETQSPRGRIALMKAV
CCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHCCCHHHCCCCHHHHHHHHH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CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]