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The map label for this gene is glcD [H]

Identifier: 138894045

GI number: 138894045

Start: 422125

End: 423537

Strand: Direct

Name: glcD [H]

Synonym: GTNG_0371

Alternate gene names: 138894045

Gene position: 422125-423537 (Clockwise)

Preceding gene: 138894044

Following gene: 138894046

Centisome position: 11.89

GC content: 50.04

Gene sequence:

>1413_bases
TTGATTCCAAACGAAGCGAAAAAACGGTTGATTCAAATTGTCGGAGCGGAAAATTACGATGATACGAAGGCTGGGCGGCT
CGTTTATTCCTACGATGCGACGCCACAGTTCCAATCGCTTCCCGATGCAGTTGTTGCACCCCGAAATACACAAGAGGTGG
CGGAAATTGCGAAAATTTGCAATGAATATCGAATTCCGATCGTACCGCGTGGATCGGGAACGAACCTTTGTGCGGGTACA
TGTCCAGTTGAAGGCGGTATCGTCCTGCTGTTTAAACATATGAACCGCATTTTAGAAATTGATGAAGAAAACTTGACGGC
GACTGTTCAACCAGGGGTGATCACGCTCGACCTCATGAGAGCCGTTGAGGAAAAAGGGTTGTTTTATCCACCAGATCCCG
GGTCGATGAAAATCTCGACGATCGGCGGCAACATTAGTGAAAATTCCGGCGGCTTGCGAGGTCTCAAATATGGAGTGACA
CGCGATTATGTACTCGGATTAGAAGTCGTCTTGGCAAATGGCGATGTCATCCGCACGGGCGGCAAGCTGGCAAAAGATGT
GGCTGGCTATGACTTAACTCGTCTGCTTGTCGGAGCGGAAGGGACGCTTGGCATTGTTACCGAAGCAACGTTGAAACTGA
TCCCCATGCCAGAGACGAAACAGACGATGCTTGCTCTTTACCAAGATTTAGAGGCAGCTGCCCGTTCTGTATCGAAAATC
ATTGCCAATCAAATCATCCCCGCAACGCTTGAGTTTTTAGACCAACCGACGTTGGAAGTTGTTGAGGACTACGCTCGCAT
CGGTTTGCCGACCGATGTGAAAGCCGTTTTGCTCATTGAACAGGATGGGCCAAAAGAAGTCGTGGAACGCGATATGAAAA
AGATGGCTGATATTTGCCGCTCAGAGCAAGCTGTGTCTGTTGAACTTGCCCGCTCAGAGGAAGAGGCTGAGGCGCTGCGG
ACAGCGAGACGAGCGGCGTTATCGGCACTGGCTCGCTTGAAACCGACGACGATTTTAGAAGATGCGACCGTGCCACGTTC
GGAAATTGCAAAAATGGTGAAAGCCATCAATGAGATCGCAGCGAAATATCAACTCAAAATTTGTACGTTCGGACATGCTG
GCGATGGCAACTTGCATCCAACCTGTCCGACCGATGCCCGCAACCGCGAGGAAATGGAGCGGGTAGAAAAAGCGTTTGAA
GAAATTTTCGCCAAAGCGATTGAATTAGGAGGAACGATCACTGGTGAACACGGTGTCGGAGCGATGAAAGCACCGTATCT
GGAATGGAAGCTTGGACCGGCTGGCATTGCTGCCATGAAAGCGATCAAACAGGCGCTCGACCCGAACAACATTATGAATC
CAGGCAAAGTGTTCGCTAAAAGCACGAGAAAACGGGTGGTGGTGACACGATGA

Upstream 100 bases:

>100_bases
CGGAGGGGGACGAGGCTTGGCGAAGGTGGGCAAGAGGCGCCAAGCCCGGCCCCTTTTCCGCTGTCCATGAGCATCAACCA
CTTTGGAAGAGGTGAGACAG

Downstream 100 bases:

>100_bases
CAACGAAGACAGAACAAGCGCACATTCAAAAAGCGTTTCAAGAACGGATGGATGAGGATGAATTAATGAACTGCATGCGC
TGTGGTTTTTGTTTGCCGTC

Product: glycolate oxidase subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MIPNEAKKRLIQIVGAENYDDTKAGRLVYSYDATPQFQSLPDAVVAPRNTQEVAEIAKICNEYRIPIVPRGSGTNLCAGT
CPVEGGIVLLFKHMNRILEIDEENLTATVQPGVITLDLMRAVEEKGLFYPPDPGSMKISTIGGNISENSGGLRGLKYGVT
RDYVLGLEVVLANGDVIRTGGKLAKDVAGYDLTRLLVGAEGTLGIVTEATLKLIPMPETKQTMLALYQDLEAAARSVSKI
IANQIIPATLEFLDQPTLEVVEDYARIGLPTDVKAVLLIEQDGPKEVVERDMKKMADICRSEQAVSVELARSEEEAEALR
TARRAALSALARLKPTTILEDATVPRSEIAKMVKAINEIAAKYQLKICTFGHAGDGNLHPTCPTDARNREEMERVEKAFE
EIFAKAIELGGTITGEHGVGAMKAPYLEWKLGPAGIAAMKAIKQALDPNNIMNPGKVFAKSTRKRVVVTR

Sequences:

>Translated_470_residues
MIPNEAKKRLIQIVGAENYDDTKAGRLVYSYDATPQFQSLPDAVVAPRNTQEVAEIAKICNEYRIPIVPRGSGTNLCAGT
CPVEGGIVLLFKHMNRILEIDEENLTATVQPGVITLDLMRAVEEKGLFYPPDPGSMKISTIGGNISENSGGLRGLKYGVT
RDYVLGLEVVLANGDVIRTGGKLAKDVAGYDLTRLLVGAEGTLGIVTEATLKLIPMPETKQTMLALYQDLEAAARSVSKI
IANQIIPATLEFLDQPTLEVVEDYARIGLPTDVKAVLLIEQDGPKEVVERDMKKMADICRSEQAVSVELARSEEEAEALR
TARRAALSALARLKPTTILEDATVPRSEIAKMVKAINEIAAKYQLKICTFGHAGDGNLHPTCPTDARNREEMERVEKAFE
EIFAKAIELGGTITGEHGVGAMKAPYLEWKLGPAGIAAMKAIKQALDPNNIMNPGKVFAKSTRKRVVVTR
>Mature_470_residues
MIPNEAKKRLIQIVGAENYDDTKAGRLVYSYDATPQFQSLPDAVVAPRNTQEVAEIAKICNEYRIPIVPRGSGTNLCAGT
CPVEGGIVLLFKHMNRILEIDEENLTATVQPGVITLDLMRAVEEKGLFYPPDPGSMKISTIGGNISENSGGLRGLKYGVT
RDYVLGLEVVLANGDVIRTGGKLAKDVAGYDLTRLLVGAEGTLGIVTEATLKLIPMPETKQTMLALYQDLEAAARSVSKI
IANQIIPATLEFLDQPTLEVVEDYARIGLPTDVKAVLLIEQDGPKEVVERDMKKMADICRSEQAVSVELARSEEEAEALR
TARRAALSALARLKPTTILEDATVPRSEIAKMVKAINEIAAKYQLKICTFGHAGDGNLHPTCPTDARNREEMERVEKAFE
EIFAKAIELGGTITGEHGVGAMKAPYLEWKLGPAGIAAMKAIKQALDPNNIMNPGKVFAKSTRKRVVVTR

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=454, Percent_Identity=33.920704845815, Blast_Score=268, Evalue=7e-72,
Organism=Homo sapiens, GI37595754, Length=477, Percent_Identity=32.2851153039832, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI119964728, Length=424, Percent_Identity=29.9528301886792, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI4501993, Length=228, Percent_Identity=31.140350877193, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1789351, Length=428, Percent_Identity=37.1495327102804, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1787977, Length=511, Percent_Identity=27.3972602739726, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI48994907, Length=484, Percent_Identity=25.8264462809917, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI48994914, Length=185, Percent_Identity=31.8918918918919, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71992373, Length=459, Percent_Identity=33.5511982570806, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI71992381, Length=459, Percent_Identity=33.1154684095861, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71992392, Length=367, Percent_Identity=34.3324250681199, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17534361, Length=445, Percent_Identity=29.8876404494382, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17556096, Length=502, Percent_Identity=25.2988047808765, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320027, Length=467, Percent_Identity=31.2633832976445, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6320764, Length=446, Percent_Identity=28.6995515695067, Blast_Score=186, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6320023, Length=453, Percent_Identity=27.1523178807947, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI18921117, Length=435, Percent_Identity=31.0344827586207, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24639277, Length=435, Percent_Identity=31.0344827586207, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24639275, Length=435, Percent_Identity=31.0344827586207, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24653753, Length=462, Percent_Identity=24.4588744588745, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 51019; Mature: 51019

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPNEAKKRLIQIVGAENYDDTKAGRLVYSYDATPQFQSLPDAVVAPRNTQEVAEIAKIC
CCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHCCCCHHCCCCCHHHHHHHHHHH
NEYRIPIVPRGSGTNLCAGTCPVEGGIVLLFKHMNRILEIDEENLTATVQPGVITLDLMR
HHCCCCEEECCCCCCEEEECCCCCCCEEHHHHHHHHHHEECCCCCEEEECCCEEHHHHHH
AVEEKGLFYPPDPGSMKISTIGGNISENSGGLRGLKYGVTRDYVLGLEVVLANGDVIRTG
HHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCHHHHEEEEEEEECCCEEECC
GKLAKDVAGYDLTRLLVGAEGTLGIVTEATLKLIPMPETKQTMLALYQDLEAAARSVSKI
CHHHHHHCCCHHHHHHCCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IANQIIPATLEFLDQPTLEVVEDYARIGLPTDVKAVLLIEQDGPKEVVERDMKKMADICR
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHC
SEQAVSVELARSEEEAEALRTARRAALSALARLKPTTILEDATVPRSEIAKMVKAINEIA
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHH
AKYQLKICTFGHAGDGNLHPTCPTDARNREEMERVEKAFEEIFAKAIELGGTITGEHGVG
HHEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
AMKAPYLEWKLGPAGIAAMKAIKQALDPNNIMNPGKVFAKSTRKRVVVTR
CCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MIPNEAKKRLIQIVGAENYDDTKAGRLVYSYDATPQFQSLPDAVVAPRNTQEVAEIAKIC
CCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHCCCCHHCCCCCHHHHHHHHHHH
NEYRIPIVPRGSGTNLCAGTCPVEGGIVLLFKHMNRILEIDEENLTATVQPGVITLDLMR
HHCCCCEEECCCCCCEEEECCCCCCCEEHHHHHHHHHHEECCCCCEEEECCCEEHHHHHH
AVEEKGLFYPPDPGSMKISTIGGNISENSGGLRGLKYGVTRDYVLGLEVVLANGDVIRTG
HHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCHHHHEEEEEEEECCCEEECC
GKLAKDVAGYDLTRLLVGAEGTLGIVTEATLKLIPMPETKQTMLALYQDLEAAARSVSKI
CHHHHHHCCCHHHHHHCCCCCCEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
IANQIIPATLEFLDQPTLEVVEDYARIGLPTDVKAVLLIEQDGPKEVVERDMKKMADICR
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHC
SEQAVSVELARSEEEAEALRTARRAALSALARLKPTTILEDATVPRSEIAKMVKAINEIA
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHH
AKYQLKICTFGHAGDGNLHPTCPTDARNREEMERVEKAFEEIFAKAIELGGTITGEHGVG
HHEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
AMKAPYLEWKLGPAGIAAMKAIKQALDPNNIMNPGKVFAKSTRKRVVVTR
CCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]